Tremella fuciformis Berk(TF)is an edible fungus with multiple nutritional values,renowned for its antiinflammatory and immunomodulatory properties.This research aimed to elucidate the mechanism by which TF regulates d...Tremella fuciformis Berk(TF)is an edible fungus with multiple nutritional values,renowned for its antiinflammatory and immunomodulatory properties.This research aimed to elucidate the mechanism by which TF regulates depressive symptoms in chronic unpredictable mild stress(CUMS)mice.CUMS modeling was conducted on 6-week-old C57BL/6 male mice,which were subsequently evaluated for depression-like behavior.Immunofluorescence(IF)staining,intestinal microbiota analysis,metabolomics analysis,and Western blot were utilized to investigate the underlying mechanisms.TF significantly mitigated depression-like behaviors and reversed the reduction of synaptophysin(SYN)and postsynaptic density-95(PSD-95)in the brain hippocampus.Furthermore,the expression level of brain-derived neurotrophic factor(BDNF)was significantly upregulated.The antidepressant effect of TF may be ascribed to its anti-inflammatory properties.Further investigation disclosed that TF significantly repressed microglia and astrocyte activation,as well as the phosphorylation of nuclear transcription factor-κB(NF-κB)and inhibitor of NF-κB(IκB)in the brain,while reducing the production of interleukin-6(IL-6),interleukin-1 beta(IL-1β)and tumor necrosis factor-alpha(TNF-α).TF may modulate neuroinflammation by inhibiting NF-κB signaling,thereby exerting an antidepressant effect,suggesting TF as a potential candidate for the prevention and treatment of depression.展开更多
文摘目的探讨水杨酸钠诱导耳鸣大鼠海马中肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)和N-甲基-D-天冬氨酸受体2B亚基(N-methyl-D-aspartate receptor 2B,NMDAR2B)的表达变化与大鼠耳鸣之间的联系。方法将18只健康成年SD大鼠随机分为三组:对照组(腹腔注射生理盐水)、腹腔注射水杨酸钠14天组(S14组)和腹腔注射水杨酸钠14天后停药恢复7天组(R7组),每组6只。检测各组大鼠造模前和造模结束后的惊跳反射前脉冲抑制(prepulse inhibition,PPI)和听性脑干反应(auditory brainstem response,ABR);检测后处死大鼠并迅速分离出海马组织,采用荧光定量PCR(quantitative PCR,qPCR)和Western Blot检测各组大鼠海马中TNF-α、IL-1β和NMDAR2B的表达。结果①S14组大鼠前脉冲抑制率较对照组显著增加(P<0.001),提示耳鸣模型建立成功;②S14组大鼠的ABR反应阈为51±1.87 dB SPL,较对照组(36.67±1.05 dB SPL)显著升高(P<0.01);R7组大鼠的ABR反应阈为35±1.29 dB SPL,与对照组差异无统计学意义(P>0.05);③S14组大鼠海马TNF-α、IL-1β和NMDAR2B的表达水平较对照组显著升高(P<0.01);与对照组相比,R7组大鼠海马IL-1β的表达水平显著升高(P<0.05),TNF-α和NMDAR2B的表达水平与对照组相比差异无统计学意义(P>0.05)。结论腹腔注射水杨酸钠可诱导大鼠产生耳鸣,并可能通过上调大鼠海马区TNF-α、IL-1β和NMDAR2B的表达水平参与耳鸣的发生发展。
基金funded by the earmarked fund for Edible Mushroom(CARS-20).
文摘Tremella fuciformis Berk(TF)is an edible fungus with multiple nutritional values,renowned for its antiinflammatory and immunomodulatory properties.This research aimed to elucidate the mechanism by which TF regulates depressive symptoms in chronic unpredictable mild stress(CUMS)mice.CUMS modeling was conducted on 6-week-old C57BL/6 male mice,which were subsequently evaluated for depression-like behavior.Immunofluorescence(IF)staining,intestinal microbiota analysis,metabolomics analysis,and Western blot were utilized to investigate the underlying mechanisms.TF significantly mitigated depression-like behaviors and reversed the reduction of synaptophysin(SYN)and postsynaptic density-95(PSD-95)in the brain hippocampus.Furthermore,the expression level of brain-derived neurotrophic factor(BDNF)was significantly upregulated.The antidepressant effect of TF may be ascribed to its anti-inflammatory properties.Further investigation disclosed that TF significantly repressed microglia and astrocyte activation,as well as the phosphorylation of nuclear transcription factor-κB(NF-κB)and inhibitor of NF-κB(IκB)in the brain,while reducing the production of interleukin-6(IL-6),interleukin-1 beta(IL-1β)and tumor necrosis factor-alpha(TNF-α).TF may modulate neuroinflammation by inhibiting NF-κB signaling,thereby exerting an antidepressant effect,suggesting TF as a potential candidate for the prevention and treatment of depression.
