[Objectives]This study was conducted to investigate the effect of lipopolysaccharide(LPS)on the gene expression levels of neuromedin B(NMB)and its receptor(NMBR)in the hypothalamic-pituitary-testis(HPT)axis of male ra...[Objectives]This study was conducted to investigate the effect of lipopolysaccharide(LPS)on the gene expression levels of neuromedin B(NMB)and its receptor(NMBR)in the hypothalamic-pituitary-testis(HPT)axis of male rabbits.[Methods]Forty male New Zealand white rabbits were randomly divided into an experimental group injected with LPS and a control group.At 0,1.5,3,6 and 12 h after LPS injection,four rabbits in each group were sacrificed,and the hypothalamus,pituitary and testicular tissues were collected.RT-PCR was carried out to detect the expression of rabbit NMB and NMBR mRNA in the HPT axis.[Results]NMB and NMBR mRNA were expressed in the hypothalamus,pituitary gland and testis of rabbits,and there was a certain time effect,indicating that the NMB/NMBR system is involved in the activities of the hypothalamus-pituitary-testis(HPT)axis.At 1.5 h after LPS stimulation,the expression of NMB mRNA in the hypothalamus increased significantly,and showed an extremely significant difference from the control group(P<0.01),while the expression of NMBR mRNA in the hypothalamus increased,but was not significantly different from the control group(P>0.05).The expression levels of NMB and NMBR mRNA in the pituitary and testis tissues in the HPT axis at different time after the LPS treatment were not significantly different from those in the control group(P>0.05).Therefore,LPS stimulation still has a certain effect on the expression of NMB and its receptor mRNA in the HPT axis.[Conclusions]This study is of great significance for the in-depth study on the mechanism of damage caused by immune stress to livestock and poultry reproduction.展开更多
为了研究注射外源性神经介素B(NMB)对小鼠睾丸中NMB及其受体和睾酮合成关键酶(St AR、CYP11A1和3β-HSD)表达及血清中睾酮浓度的影响,试验将40只雄性ICR小鼠随机分成12 h对照组(12 h Control组)、12 h试验组(12 h NMB组)、24 h对照组(24...为了研究注射外源性神经介素B(NMB)对小鼠睾丸中NMB及其受体和睾酮合成关键酶(St AR、CYP11A1和3β-HSD)表达及血清中睾酮浓度的影响,试验将40只雄性ICR小鼠随机分成12 h对照组(12 h Control组)、12 h试验组(12 h NMB组)、24 h对照组(24 h Control组)和24 h试验组(24 h NMB组),每组10只,2个对照组和2个试验组分别按体重10μL/g腹腔注射生理盐水和10 nmol/L NMB溶液;称量注射前后小鼠体重,计算饲料用量,并观察小鼠行为特征;各组小鼠分别于注射后12小时、24小时摘眼球采血并处死取睾丸,通过ELISA法检测小鼠血清中睾酮的浓度;通过实时荧光定量PCR(q PCR)和蛋白印迹法(Western-blot)检测小鼠睾丸中NMB、NMBR、St AR、CYP11A1和3β-HSD m RNA和蛋白表达量。结果表明:与12 h Control组相比,12 h NMB组小鼠行为无明显异常,体重增量、总摄食量略有减少,料重比略有增高,但差异不显著(P>0.05);NMB、NMBR、St AR、CYP11A1和3β-HSD m RNA及蛋白表达量均差异不显著(P>0.05);血清中睾酮浓度差异不显著(P>0.05)。与24 h Control组相比,24 h NMB组小鼠被毛粗乱、脾气暴躁,体重增量显著减少(P<0.05);总摄食量减少,料重比略有增高,但均差异不显著(P>0.05);NMB m RNA和蛋白表达量极显著降低(P<0.01),NMBR、St AR、CYP11A1和3β-HSD m RNA及蛋白表达量均差异不显著(P>0.05);血清中睾酮浓度差异不显著(P>0.05)。与12 h NMB组相比,24 h NMB组NMBR m RNA表达量极显著降低(P<0.01),24 h NMB组NMBR蛋白表达量显著增加(P<0.05)。说明小鼠腹腔注射NMB能够抑制小鼠摄食,但对小鼠睾丸中睾酮的合成和分泌影响不大。展开更多
基金National Natural Science Foundation of China(31802149,31372388)Natural Science Foundation of Jiangsu Province(BK20180919)Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。
文摘[Objectives]This study was conducted to investigate the effect of lipopolysaccharide(LPS)on the gene expression levels of neuromedin B(NMB)and its receptor(NMBR)in the hypothalamic-pituitary-testis(HPT)axis of male rabbits.[Methods]Forty male New Zealand white rabbits were randomly divided into an experimental group injected with LPS and a control group.At 0,1.5,3,6 and 12 h after LPS injection,four rabbits in each group were sacrificed,and the hypothalamus,pituitary and testicular tissues were collected.RT-PCR was carried out to detect the expression of rabbit NMB and NMBR mRNA in the HPT axis.[Results]NMB and NMBR mRNA were expressed in the hypothalamus,pituitary gland and testis of rabbits,and there was a certain time effect,indicating that the NMB/NMBR system is involved in the activities of the hypothalamus-pituitary-testis(HPT)axis.At 1.5 h after LPS stimulation,the expression of NMB mRNA in the hypothalamus increased significantly,and showed an extremely significant difference from the control group(P<0.01),while the expression of NMBR mRNA in the hypothalamus increased,but was not significantly different from the control group(P>0.05).The expression levels of NMB and NMBR mRNA in the pituitary and testis tissues in the HPT axis at different time after the LPS treatment were not significantly different from those in the control group(P>0.05).Therefore,LPS stimulation still has a certain effect on the expression of NMB and its receptor mRNA in the HPT axis.[Conclusions]This study is of great significance for the in-depth study on the mechanism of damage caused by immune stress to livestock and poultry reproduction.
文摘为了研究注射外源性神经介素B(NMB)对小鼠睾丸中NMB及其受体和睾酮合成关键酶(St AR、CYP11A1和3β-HSD)表达及血清中睾酮浓度的影响,试验将40只雄性ICR小鼠随机分成12 h对照组(12 h Control组)、12 h试验组(12 h NMB组)、24 h对照组(24 h Control组)和24 h试验组(24 h NMB组),每组10只,2个对照组和2个试验组分别按体重10μL/g腹腔注射生理盐水和10 nmol/L NMB溶液;称量注射前后小鼠体重,计算饲料用量,并观察小鼠行为特征;各组小鼠分别于注射后12小时、24小时摘眼球采血并处死取睾丸,通过ELISA法检测小鼠血清中睾酮的浓度;通过实时荧光定量PCR(q PCR)和蛋白印迹法(Western-blot)检测小鼠睾丸中NMB、NMBR、St AR、CYP11A1和3β-HSD m RNA和蛋白表达量。结果表明:与12 h Control组相比,12 h NMB组小鼠行为无明显异常,体重增量、总摄食量略有减少,料重比略有增高,但差异不显著(P>0.05);NMB、NMBR、St AR、CYP11A1和3β-HSD m RNA及蛋白表达量均差异不显著(P>0.05);血清中睾酮浓度差异不显著(P>0.05)。与24 h Control组相比,24 h NMB组小鼠被毛粗乱、脾气暴躁,体重增量显著减少(P<0.05);总摄食量减少,料重比略有增高,但均差异不显著(P>0.05);NMB m RNA和蛋白表达量极显著降低(P<0.01),NMBR、St AR、CYP11A1和3β-HSD m RNA及蛋白表达量均差异不显著(P>0.05);血清中睾酮浓度差异不显著(P>0.05)。与12 h NMB组相比,24 h NMB组NMBR m RNA表达量极显著降低(P<0.01),24 h NMB组NMBR蛋白表达量显著增加(P<0.05)。说明小鼠腹腔注射NMB能够抑制小鼠摄食,但对小鼠睾丸中睾酮的合成和分泌影响不大。
