As an acidic glycoprotein,carcinoembryonic antigen(CEA)is of great value as a broad-spectrum tumor marker in the differential diagnosis and surveillance of malignant tumors.In this work,we report an electrochemical ap...As an acidic glycoprotein,carcinoembryonic antigen(CEA)is of great value as a broad-spectrum tumor marker in the differential diagnosis and surveillance of malignant tumors.In this work,we report an electrochemical aptasensor for the ultrasensitive and highly selective detection of CEA,taking advantage of the dual amplification by the boronate affinity-assisted electrochemically controlled atom transfer radical polymerization(BA-eATRP).Specifically,the BAeATRP-based electrochemical aptasensing of CEA involves the capture of target antigens by nucleic acid aptamers,the covalent crosslinking of ATRP initiators to CEA antigens via the selective interactions between the phenylboronic acid(PBA)group and the cis-diol group of the monosaccharide residues,and the collection of the ferrocene(Fc)reporters via the eATRP of ferrocenylmethyl methacrylate(FcMMA).As CEA is decorated with hundreds of cis-diol groups,the BA-based crosslinking can result in the labeling of each CEA with hundreds of ATRP initiators;furthermore,the eATRP of FcMMA results in the surface-initiated growth of long-chain ferrocenyl polymers,leading to the tethering of each ATRP initiator-conjugated site with hundreds to thousands of Fc reporters.Such that,the BAeATRP can result in the efficient labeling of each CEA with a plenty of Fc reporters.Under the optimized conditions,the BA-eATRP-based strategy enables the highly selective aptasensing of CEA at a concentration as low as 0.34 pg·mL^(-1),with a linear range of 1.01000 pg·mL^(-1).Besides,this aptasensor has been successfully applied to the quantitative analysis of CEA in human serum.The BA-eATRP-based electrochemical aptasensor is cost-effective and simple in operation,holding broad application prospect in the ultrasensitive and highly selective detection of CEA.展开更多
Reactive oxygen signaling regulates numerous biological processes,including stress responses in plants.Redox sensors transduce reactive oxygen signals into cellular responses.Here,we present biochemical evidence that ...Reactive oxygen signaling regulates numerous biological processes,including stress responses in plants.Redox sensors transduce reactive oxygen signals into cellular responses.Here,we present biochemical evidence that a plant quiescin sulfhydryl oxidase homolog(QSOX1)is a redox sensor that negatively regulates plant immunity against a bacterial pathogen.The expression level of QSOX1 is inversely correlated with pathogen-induced reactive oxygen species(ROS)accumulation.Interestingly,QSOX1 both senses and regulates ROS levels by interactingn with and mediating redox regulation of S-nitrosoglutathione reductase,which,consistent with previous findings,influences reactive nitrogen-mediated regulation of ROS generation.Collectively,our data indicate that QSOX1 is a redox sensorthat negatively regulates plant immunity by linking reactive oxygen and reactive nitrogen signaling to limit ROS production.展开更多
基金This work was co-supported by the Guangdong Basic and Applied Basic Research Foundation(No.2022A1515010618)the Young Talent Support Project of Guangzhou Association for Science and Technology(No.QT-2023-009)+2 种基金the National Natural Science Foundation of China(No.21904026,21974031)the Guangzhou Science and Technology Project(No.202201010600,202201020170,202201000002)the Innovation Training Program for College Students of Guangzhou University(No.202211078113,S202111078031).
文摘As an acidic glycoprotein,carcinoembryonic antigen(CEA)is of great value as a broad-spectrum tumor marker in the differential diagnosis and surveillance of malignant tumors.In this work,we report an electrochemical aptasensor for the ultrasensitive and highly selective detection of CEA,taking advantage of the dual amplification by the boronate affinity-assisted electrochemically controlled atom transfer radical polymerization(BA-eATRP).Specifically,the BAeATRP-based electrochemical aptasensing of CEA involves the capture of target antigens by nucleic acid aptamers,the covalent crosslinking of ATRP initiators to CEA antigens via the selective interactions between the phenylboronic acid(PBA)group and the cis-diol group of the monosaccharide residues,and the collection of the ferrocene(Fc)reporters via the eATRP of ferrocenylmethyl methacrylate(FcMMA).As CEA is decorated with hundreds of cis-diol groups,the BA-based crosslinking can result in the labeling of each CEA with hundreds of ATRP initiators;furthermore,the eATRP of FcMMA results in the surface-initiated growth of long-chain ferrocenyl polymers,leading to the tethering of each ATRP initiator-conjugated site with hundreds to thousands of Fc reporters.Such that,the BAeATRP can result in the efficient labeling of each CEA with a plenty of Fc reporters.Under the optimized conditions,the BA-eATRP-based strategy enables the highly selective aptasensing of CEA at a concentration as low as 0.34 pg·mL^(-1),with a linear range of 1.01000 pg·mL^(-1).Besides,this aptasensor has been successfully applied to the quantitative analysis of CEA in human serum.The BA-eATRP-based electrochemical aptasensor is cost-effective and simple in operation,holding broad application prospect in the ultrasensitive and highly selective detection of CEA.
基金supported by grants from the"BioGreen21 Agri-Tech Inovation Program(project no.PJ015824 to S.Y.L.and PJ0159992021 to M.G.K.)",Rural Development Administration(RDA),South Koreaby the Basic Science Research Program through the National Research Foundation(NRF)of South Korea funded by the Ministry of Education(NRF-2018R1A6A3A11049525 to H.B.C.).
文摘Reactive oxygen signaling regulates numerous biological processes,including stress responses in plants.Redox sensors transduce reactive oxygen signals into cellular responses.Here,we present biochemical evidence that a plant quiescin sulfhydryl oxidase homolog(QSOX1)is a redox sensor that negatively regulates plant immunity against a bacterial pathogen.The expression level of QSOX1 is inversely correlated with pathogen-induced reactive oxygen species(ROS)accumulation.Interestingly,QSOX1 both senses and regulates ROS levels by interactingn with and mediating redox regulation of S-nitrosoglutathione reductase,which,consistent with previous findings,influences reactive nitrogen-mediated regulation of ROS generation.Collectively,our data indicate that QSOX1 is a redox sensorthat negatively regulates plant immunity by linking reactive oxygen and reactive nitrogen signaling to limit ROS production.
