In dryland ecosystems,nitrogen(N)is the primary limiting factor after water availability,constraining both plant productivity and organic matter decomposition while also regulating ecosystem function and service provi...In dryland ecosystems,nitrogen(N)is the primary limiting factor after water availability,constraining both plant productivity and organic matter decomposition while also regulating ecosystem function and service provision.However,the distributions of different soil N fraction stocks in drylands and the factors that influence them remain poorly understood.In this study,we collected 2076 soil samples from 173 sites across the drylands of northern China during the summers of 2021 and 2022.Using the best-performing eXtreme Gradient Boosting(XGBoost)model,we mapped the spatial distributions of the soil N fraction stocks and identified the key drivers of their variability.Our findings revealed that the stocks of total nitrogen(TN),inorganic nitrogen(IN),and microbial biomass nitrogen(MBN)in the top 30 cm soil layer were 1020.4,92.2,and 40.8 Tg,respectively,with corresponding mean densities of 164.6,14.9,and 6.6 g/m2.Climate variables-particularly mean annual temperature and aridity-along with human impacts emerged as the dominant drivers of soil N stock distribution.Notably,increased aridity and intensified human impacts exerted mutually counteracting effects on soil N fractions:aridity-driven moisture limitation generally suppressed N accumulation,whereas anthropogenic activities(e.g.,fertilization and grazing)promoted N enrichment.By identifying the key environmental and anthropogenic factors shaping the soil N distribution,this study improves the accuracy of regional and global N stock estimates.These insights provide a scientific foundation for developing more effective soil N management strategies in dryland ecosystems,contributing to sustainable land use and long-term ecosystem resilience in drylands.展开更多
Soil nitrogen(N)is the main limiting nutrient for plant growth,which is sensitive to variations in the soil oxygen environment.To provide insights into plant N accumulation and yield under aerated and drip irrigation,...Soil nitrogen(N)is the main limiting nutrient for plant growth,which is sensitive to variations in the soil oxygen environment.To provide insights into plant N accumulation and yield under aerated and drip irrigation,a greenhouse tomato experiment was conducted with six treatments,including three fertilization types:inorganic fertilizer(NPK);organic fertilizer(OM);chemical(75%of applied N)+organic fertilizer(25%)(NPK+OM)under drip irrigation(DI)and aerated irrigation(AI)methods.Under Al,total soil carbon mineralization(C_(min))was significantly higher(by 5.7-7.0%)than under DI irrigation.C_(min)in the fertilizer treatments followed the order NPK+OM>OM>NPK under both AI and DI.Potentially mineralizable C(C_(0))and N(N_(0))was greater under AI than under DI.Gross N mineralization,gross nitrification,and NH_(4)^(+)immobilization rates were significantly higher under the AINPK treatment than the DINPK treatment by 2.58-3.27-,1.25-1.44-,and 1-1.26-fold,respectively.These findings demonstrated that AI and the addition of organic fertilizer accelerated the turnover of soil organic matter and N transformation processes,thereby enhancing N availability.Moreover,the combination of AI and organic fertilizer application was found to promote root growth(8.4-10.6%),increase the duration of the period of rapid N accumulation(ΔT),and increase the maximum N accumulation rate(V_(max)),subsequently encouraging aboveground dry matter accumulation.Consequently,the AI treatment yield was significantly greater(by 6.3-12.4%)than under the DI treatment.Further,N partial factor productivity(NPFP)and N harvest index(NHI)were greater under AI than under DI,by 6.3 to 12.4%,and 4.6 to 8.1%,respectively.The rankings of yield and NPFP remained consistent,with NPK+OM>OM>NPK under both AI and DI treatments.These results highlighted the positive impacts of AI and organic fertilizer application on soil N availability,N uptake,and overall crop yield in tomato.The optimal management measure was identified as the AINPK+OM treatment,which led to more efficient N management,better crop growth,higher yield,and more sustainable agricultural practices.展开更多
Nitrogen(N)and phosphorus(P)are mineral nutrients essential for plant growth and development,playing a crucial role throughout the plant life cycle.Cotton,a globally significant textile crop,has a particularly high de...Nitrogen(N)and phosphorus(P)are mineral nutrients essential for plant growth and development,playing a crucial role throughout the plant life cycle.Cotton,a globally significant textile crop,has a particularly high demand for N fertilizer across its developmental stages.This review explores the effects of adequate or deficient N and P levels on cotton growth phases,focusing on their influence on physiological processes and molecular mechanisms.Key topics include the regulation of N-and P-related enzymes,hormones,and genes,as well as the complex interplay of N-and P-related signaling pathways from the aspects of N-P signaling integration to regulate root development,N-P signaling integration to regulate nutrient uptake,and regulation of N-P interactions—a frontier in current research.Strategies for improving N and P use efficiency are also discussed,including developing high-efficiency cotton cultivars and identifying functional genes to enhance productivity.Generally speaking,we take model plants as a reference in the hope of coming up with new strategies for the efficient utilization of N and P in cotton.展开更多
Nitrogen is one of the most important elements that can limit plant growth in forest ecosystems. Studies of nitrogen mineralization, nitrogen saturation and nitrogen cycle in forest ecosystems is very necessary for un...Nitrogen is one of the most important elements that can limit plant growth in forest ecosystems. Studies of nitrogen mineralization, nitrogen saturation and nitrogen cycle in forest ecosystems is very necessary for understanding the productivity of stand, nutrient cycle and turnover of nitrogen of forest ecosystems. Based on comparison and analysis of domestic and in-ternational academic references related to studies on nitrogen mineralization, nitrogen saturation and nitrogen cycle in recent 10 years, the current situation and development of the study on these aspects, and the problems existed in current researches were reviewed. At last, some advices were given for future researches.展开更多
The increase in soil temperature associated with climate change has introduced considerable challenges to crop production.Split nitrogen application(SN)represents a potential strategy for improving crop nitrogen use e...The increase in soil temperature associated with climate change has introduced considerable challenges to crop production.Split nitrogen application(SN)represents a potential strategy for improving crop nitrogen use efficiency and enhancing crop stress resistance.Nevertheless,the precise interaction between soil warming(SW)and SN remains unclear.In order to ascertain the impact of SW on maize growth and whether SN can improve the tolerance of maize to SW,a two-year field experiment was conducted(2022-2023).The aim was to examine the influence of two SW ranges(MT,warming 1.40℃;HT,warming 2.75℃)and two nitrogen application methods(N1,one-time basal application of nitrogen fertilizer;N2,one third of base nitrogen fertilizer+two thirds of jointing stage supplemental nitrogen fertilizer)on maize root growth,photosynthetic characteristics,nitrogen use efficiency,and yield.The results demonstrated that SW impeded root growth and precipitated the premature aging of maize leaves following anthesis,particularly in the HT,which led to a notable reduction in maize yield.In comparison to N1,SN has been shown to increase root length density by 8.54%,root bleeding rate by 8.57%,and enhance root distribution ratio in the middle soil layers(20-60 cm).The interaction between SW and SN had a notable impact on maize growth and yield.The SN improved the absorption and utilization efficiency of nitrogen by promoting root development and downward canopy growth,thus improving the tolerance of maize to SW at the later stage of growth.In particular,the N2HT resulted in a 14.51%increase in the photosynthetic rate,a 18.58%increase in nitrogen absorption efficiency,and a 18.32%increase in maize yield compared with N1HT.It can be posited that the SN represents a viable nitrogen management measure with the potential to enhance maize tolerance to soil high-temperature stress.展开更多
The soybean, cotton, maize and sorghum were planted in pot under low nitrogen, high nitrogen treatments, the soil available nitrogen constitution and con- version and utilization of nitrogen fertilizer were determined...The soybean, cotton, maize and sorghum were planted in pot under low nitrogen, high nitrogen treatments, the soil available nitrogen constitution and con- version and utilization of nitrogen fertilizer were determined, so as to provide techni- cal guidance for reasonable use and improving use efficiency of nitrogen fertilizer for different types of crops. Compared with the control with nitrogen but unplanted crop, growing soybean, cotton, maize, sorghum significantly decreased the soil available N contents by 53. 48%, 51.54%, 33.10%, 55.03%,and influenced the constitution of soil available N. Thereinto, growing soybean, cotton, maize and sorghum significantly decreased soil inorganic N contents by 85.41%, 83.09%, 70.89% and 83.35%,but increased soil hydrolysable organic N contents by 1.41, 1.53, 2.11 and 1.28 times, respectively; growing soybean, cotton, maize and sorghum significantly decreased the rate of soil inorganic N to available N by 68.61%, 65.09%, 56.47% and 63.00%, but increased the rate of soil hydrolysable organic N to available N by 4.18, 4.21, 3.66 and 4.08 times, respectively. Compared with the control, growing soybean, cotton, maize and sorghum significantly increased the transform rate of ammonium nitrogen fertilizer by 93.66%, 38.19%, 32.58% and 38.31% respectively, and growing soybean treatment had the highest increasing range; the nitrification rates of ammo- nium nitrogen fertilizer of growing soybean, cotton, maize and sorghum treatments were negative values, and growing soybean treatment had the highest decreasing amplitude. The ammonium nitrogen fertilizer use efficiency of growing soybean, cot- ton, maize and sorghum treatments were 52.01%, 28.31%, 24.16% and 28.40% re- spectively and growing soybean treatment had the highest value. In conclusion, growing crops suppressed the soil nitrification and accelerated the development of soil hydrolysable organic nitrogen by the utilization of soil available nitrogen and the alteration of soil environment, and hence impacted the constitution of soil available nitrogen and the transform and use of ammonium nitrogen applied in soil. Legumi- nous crops had stronger ability of suppressing nitrification, making use of ammonium compared with non-Leguminous crops.展开更多
Nitrogen(N)uptake is regulated by water availability,and a water deficit can limit crop N responses by reducing N uptake and utilization.The complex and multifaceted interplay between water availability and the crop N...Nitrogen(N)uptake is regulated by water availability,and a water deficit can limit crop N responses by reducing N uptake and utilization.The complex and multifaceted interplay between water availability and the crop N response makes it difficult to predict and quantify the effect of water deficit on crop N status.The nitrogen nutrition index(NNI)has been widely used to accurately diagnose crop N status and to evaluate the effectiveness of N application.The decline of NNI under water-limiting conditions has been documented,although the underlying mechanism governing this decline is not fully understood.This study aimed to elucidate the reason for the decline of NNI under waterlimiting conditions and to provide insights into the accurate utilization of NNI for assessing crop N status under different water-N interaction treatments.Rainout shelter experiments were conducted over three growing seasons from 2018 to 2021 under different N(75 and 225 kg N ha^(-1),low N and high N)and water(120 to 510 mm,W0 to W3)co-limitation treatments.Plant N accumulation,shoot biomass(SB),plant N concentration(%N),soil nitrate-N content,actual evapotranspiration(ET_a),and yield were recorded at the stem elongation,booting,anthesis and grain filling stages.Compared to W0,W1 to W3 treatments exhibited NNI values that were greater by 10.2 to 20.5%,12.6to 24.8%,14 to 24.8%,and 16.8 to 24.8%at stem elongation,booting,anthesis,and grain filling,respectively,across the 2018-2021 seasons.This decline in NNI under water-limiting conditions stemmed from two main factors.First,reduced ET_(a) and SB led to a greater critical N concentration(%N_(c))under water-limiting conditions,which contributed to the decline in NNI primarily under high N conditions.Second,changes in plant%N played a more significant role under low N conditions.Plant N accumulation exhibited a positive allometric relationship with SB and a negative relationship with soil nitrate-N content under water-limiting conditions,indicating co-regulation by SB and the soil nitrate-N content.However,this regulation was influenced by water availability.Plant N accumulation sourced from the soil nitrate-N content reflects soil N availability.Greater soil water availability facilitated greater absorption of soil nitrate-N into the plants,leading to a positive correlation between plant N accumulation and ET_(a)across the different water-N interaction treatments.Therefore,considering the impact of soil water availability is crucial when assessing soil N availability under water-limiting conditions.The findings of this study provide valuable insights into the factors contributing to the decline in NNI among different water-N interaction treatments and can contribute to the more accurate utilization of NNI for assessing winter wheat N status.展开更多
[Objective]The paper was to study the effect of different nitrogen application levels and modes on contents of different forms of nitrogen in rice plants.[Method] Using Guichao 2 and Diejiazhan as test materials,with ...[Objective]The paper was to study the effect of different nitrogen application levels and modes on contents of different forms of nitrogen in rice plants.[Method] Using Guichao 2 and Diejiazhan as test materials,with total nitrogen,protein nitrogen,chlorophyll nitrogen,soluble protein nitrogen,Rubisco nitrogen,free amino acid nitrogen as indicators,the effect of nitrogen application on contents of different forms of nitrogen in rice plants were studied.[Result] The contents of total nitrogen,protein nitrogen,chlorophyll nitrogen,soluble protein nitrogen,Rubisco-N,free amino nitrogen of rice plant during heading and maturity stage increased with the increasing application amount of nitrogen,and their contents would be high under heavy application of spike fertilizer when the application amount of nitrogen was the same.However,application of nitrogen had different impact on different forms of nitrogen,which showed obvious impact on the contents of Rubisco-N and free amino nitrogen.The differences of Rubisco-N and free amino nitrogen content of two varieties during heading and maturity stage under different nitrogen application modes all reached significant or extremely significant level.Correlation analysis showed that total nitrogen content of rice plant had extremely significant correlation with protein nitrogen content during heading and maturity stage in both early and late seasons.[Conclusion] The study provided a theoretical basis for clarifying the regulatory role of nitrogen application on nitrogen absorption and utilization.展开更多
Light and nitrogen(N)are two critically environmental factors essential for plant survival,as they constitute the fundamental molecular framework of plant cells and significantly influence patterns of plant growth and...Light and nitrogen(N)are two critically environmental factors essential for plant survival,as they constitute the fundamental molecular framework of plant cells and significantly influence patterns of plant growth and development.Light is the driving force behind photosynthesis,a process that converts light energy into chemical energy stored as sugars.Additionally,light acts as a direct signal that can modulate plant morphogenesis and structural development.Nitrogen,as the most crucial mineral nutrient for plants,is a component of numerous biomolecules.It also functions as a signaling molecule,regulating plant growth and development.Moreover,light and nitrogen directly regulate the balance of carbon(C)and N within plants,affecting numerous biochemical reactions and various physiological processes.This review focuses on the interactions between light and nitrogen in physiological,metabolic,and molecular levels.We will also discuss the regulatory networks and mechanisms through which light and nitrogen influence C and N absorption and metabolism in plants.展开更多
Synchronizing the nitrogen(N)supply of slow-and controlled-release N fertilizers(SCRNFs)with rice N demand is essential in replacing multiple urea applications with a single basal application of SCRNFs.Traditional ass...Synchronizing the nitrogen(N)supply of slow-and controlled-release N fertilizers(SCRNFs)with rice N demand is essential in replacing multiple urea applications with a single basal application of SCRNFs.Traditional assessment of N supply characteristics primarily examines N release patterns,which are limited to coated SCRNFs and disregard N transformation mechanisms,necessitating a more universal and reliable index.Based on the capacity of crop N status to detect N deficiency or excess,we hypothesized that utilizing leaf N balance index(NBI)as a measure of N status could offer novel insights into assessing N supply characteristics of SCRNFs.Field experiments were conducted with four individual SCRNFs-humic acid urea(HAU),sulfur-coated urea(SCU),urease inhibitor urea(UIU),and polymer-coated urea(PCU)and their four combined forms,alongside high-yield urea split application as control(CK).The results revealed that NBI dynamics relative to CK reflected the N supply potential of different SCRNFs while categorizing them as short-,medium-,and long-acting fertilizers.Combinations incorporating the long-acting SCRNF(PCU)consistently demonstrated superior performance in yield(by 5.5%)and N use efficiency(by 42.8%)through providing more consistent and efficient N supply throughout the rice growth cycle.Grain yield exhibited negative correlation with the difference in NBI dynamics between SCRNFs and CK,suggesting that synchronizing N supply between one-time application of SCRNFs and conventional high-yield fertilization is crucial for high yield.These findings demonstrate the potential of N status diagnosed by leaf NBI to evaluate N supply characteristics of SCRNFs and highlight the importance of synchronized N supply for a one-time SCRNF application.展开更多
The responses of drip-irrigated rice physiological traits to water and fertilizers have been widely studied.However,the responses of yield,root traits and their plasticity to the nitrogen environment in different nitr...The responses of drip-irrigated rice physiological traits to water and fertilizers have been widely studied.However,the responses of yield,root traits and their plasticity to the nitrogen environment in different nitrogen-efficient cultivars are not fully understood.An experiment was conducted from 2020-2022 with a high nitrogen use efficiency(high-NUE)cultivar(T-43)and a low-NUE cultivar(LX-3),and four nitrogen levels(0,150,300,and 450 kg ha^(-1))under drip irrigation in large fields.The aim was to study the relationships between root morphology,conformation,biomass,and endogenous hormone contents,yield and NUE.The results showed three main points:1)Under the same N application rate,compared with LX-3,the yield,N partial factor productivity(PFP),fine root length density(FRLD),shoot dry weight(SDW),root indole-3-acetic acid(IAA),and root zeatin and zeatin riboside(Z+ZR)of T-43 were significantly greater by11.4-18.9,11.3-13.5,11.6-15.7,9.9-31.1,6.1-48.1,and 22.8-73.6%,respectively,while the root-shoot ratio(RSR)and root abscisic acid(ABA)were significantly lower(P<0.05);2)nitrogen treatment significantly increased the rice root morphological indexes and endogenous hormone contents(P<0.05).Compared to N0,the yield,RLD,surface area density(SAD),root volume density(RVD),and root endogenous hormones(IAA,Z+ZR)were significantly increased in both cultivars under N2 by 61.6-71.6,64.2-74.0,69.9-105.6,6.67-9.91,54.0-67.8,and 51.4-58.9%,respectively.Compared with N3,the PFP and N agronomic efficiency(NAE)of nitrogen fertilizer under N2 increased by 52.3-62.4 and39.2-63.0%,respectively;3)the responses of root trait plasticity to the N environment significantly differed between the cultivars(P<0.05).Compared with LX-3,T-43 showed a longer root length and larger specific surface area,which is a strategy for adapting to changes in the nutrient environment.For the rice cultivar with high-NUE,the RSR was optimized by increasing the FRLD,root distribution in upper soil layers,and root endogenous hormones(IAA,Z+ZR)under suitable nitrogen conditions(N2).An efficient nutrient acquisition strategy can occur through root plasticity,leading to greater yield and NUE.展开更多
Long-term excessive nitrogen(N)application neither increases nor enhances grain yield and N use efficiency(NUE)of maize,yet the mechanisms involving root morphological and physiological characteristics remain unclear....Long-term excessive nitrogen(N)application neither increases nor enhances grain yield and N use efficiency(NUE)of maize,yet the mechanisms involving root morphological and physiological characteristics remain unclear.This study aimed to elucidate the mechanisms underlying stagnant grain yield under excessive N application by examining root morphological and physiological characteristics.A 10-year N fertilizer trial was conducted in Jilin Province,Northeast China,cultivating maize at three N fertilizer levels(zero N,N0;recommended N,N2;and high N level,N4)from 2019 to 2021.Two widely cultivated maize genotypes,‘Xianyu 335’(XY335)and‘Zhengdan 958’(ZD958),were evaluated.Grain yield,N content,root morphology,and physiological characteristics were analyzed to assess the relationships between N uptake,N utilization,plant growth,and root systems under different N treatments.Compared to N0,root biomass,post-silking N uptake,and grain yield improved significantly with increased N input,while no significant differences emerged between recommended N and high N.High N application enhanced root length and root surface area but decreased root activity(measured by TTC(2,3,5-triphenyltetrazolium chloride)method),nitrate reductase activity,and root activity absorbing area across genotypes.Root length and root to shoot ratio negatively affected N uptake(by-1.2 and-24.6%),while root surface area,root activity,nitrate reductase activity,and root activity absorbing area contributed positively.The interaction between cultivar and N application significantly influenced NUE.XY335 achieved the highest NUE(11.6%)and N recovery efficiency(18.4%)through superior root surface area(23.6%),root activity(12.5%),nitrate reductase activity(8.3%),and root activity absorbing area(6.9%)compared to other treatments.Recommended N application enhanced Post N uptake,NUE,and grain yield through improved root characteristics,while high N application failed to increase or decreased NUE by reducing these parameters.This study demonstrates that root surface area,root activity,nitrate reductase activity,and root activity absorbing area limit NUE increase under high N application.展开更多
Symbiotic nitrogen fixation in members of the Fabaceae family is highly efficient and beneficial for global agriculture,but not all species in this family form root nodules with rhizobial bacteria.Nodulation mainly oc...Symbiotic nitrogen fixation in members of the Fabaceae family is highly efficient and beneficial for global agriculture,but not all species in this family form root nodules with rhizobial bacteria.Nodulation mainly occurs in plants belonging to the Papilionoideae and Caesalpinioideae subfamilies(Tederso0 et al.,2018;van Velzen et al.,2019).Nodulation mechanisms in Fabaceae are well studied(Yang et al.,2022),and genomic comparisons of nodulating and non-nodulating host species can provide valuable insights into the evolutionary and genetic basis of this key process.展开更多
Nitrogen(N)is the most important nutrient for plants;however,microbe-mediated N transformation under different N forms is unclear.This experiment investigated the effects of four treatments fertilized with various N f...Nitrogen(N)is the most important nutrient for plants;however,microbe-mediated N transformation under different N forms is unclear.This experiment investigated the effects of four treatments fertilized with various N forms,no N(control,CK),100%ammonium N(AN),100%nitrate N(NN),and 50%ammonium N+50%nitrate N(ANNN),on soil chemical properties,rhizosphere bacterial network,and rice growth.The ANNN treatment enhanced soil pH by 6.9%,soil organic carbon by 12%,and microbial biomass N(MBN)by 60%compared to CK.The linear discriminant effect size(LEfSe)analysis indicated four highly abundant biomarkers of bacterial communities each in the CK,NN,and AN treatments,while the ANNN treatment showed six highly abundant biomarkers with maximum effect size and linear discriminant analysis(LDA)score>4.The 16S rRNA gene-predicted functions under PICRUST indicated glutathione metabolism and proteasome and Tax4Fun recorded amino acid metabolism in the ANNN treatment.The combination of ammonium and nitrate N(i.e.,the ANNN treatment)significantly increased the expression levels of the genes encoding N metabolism,including AMT1,NRT2.1,GS1,and GOGAT1,and induced 39%,27%,35%,and 38%increase in nitrate reductase,nitrite reductase,glutamine synthetase,and glutamate synthase,respectively,in comparison to CK.In addition,the ANNN treatment promoted rice leaf photosynthetic rate by 37%,transpiration rate by 41%,CO_(2) exchange rate by 11%,and stomatal conductance by 18%compared to CK,while increased N use efficiency(NUE)by 10%and 19%,respectively,compared to the AN and NN treatments.These findings suggest that the combination of ammonium and nitrate N can promote bacterial community abundance,composition,and functional pathways by improving soil properties and can increase NUE and rice growth.This study provides a theoretical basis for the rational application of N fertilizers and the implications of this approach for future sustainable crop production.展开更多
Polymeric nitrogen is a potential high-energy-density material with the advantages of high energy density, easy availability of raw materials, and non-pollution. The design and synthesis of polymeric nitrogen are impo...Polymeric nitrogen is a potential high-energy-density material with the advantages of high energy density, easy availability of raw materials, and non-pollution. The design and synthesis of polymeric nitrogen are important in the research field of energetic materials. The cubic gauche nitrogen was successfully synthesized at high pressure in the diamond anvil cell, which stimulated the theoretical and experimental investigations. To date, several hundred kinds of polymeric nitrogen have been reported. This review introduces the progressive development of polymeric nitrogen with high energy density, the challenges faced by the synthesized polymeric nitrogen under high-pressure,and the importance to improve the stability of polymeric nitrogen at ambient pressure. Furthermore, alternative methods for synthesizing polymeric nitrogen under moderate conditions are also presented. In this field, more efforts are needed to develop strategies for stabilizing more polymeric nitrogen to ambient conditions, especially the stability of free surfaces.展开更多
Recent studies have shown that mucilage secretion from aerial roots is an essential feature of modern maize inbred lines,with some retaining the nitrogen-fixing capabilities of ancient landraces.To explore the genetic...Recent studies have shown that mucilage secretion from aerial roots is an essential feature of modern maize inbred lines,with some retaining the nitrogen-fixing capabilities of ancient landraces.To explore the genetic basis of nitrogen fixation in mucilage and its evolution from teosinte(Zea mays ssp.mexicana)to modern maize,we developed a recombinant inbred line(RIL)population from teosinte and cultivated it under low-nitrogen conditions.Large-scale,multi-year,and multi-environment analyses of RIL-Teo,Doubled Haploid-A(DH-A),Doubled Haploid-B(DH-B),and association populations led to the identification of 15 quantitative trait loci(QTL),68 quantitative trait nucleotides(QTN),and 59 candidate genes linked to mucilage secretion from aerial roots.Functional verification of the candidate gene ZmAco3,which is associated with mucilage secretion in aerial roots,demonstrated that deletion of this gene resulted in a reduction in mucilage secretion in aerial roots.In addition,most maize inbred lines exhibited stronger mucilage secretion from aerial roots under low-nitrogen conditions than under normal-nitrogen conditions.We categorized mucilage secretion into constitutive and low-nitrogen-inducible types.Through genotype-by-environment interaction studies,8 QTL,16 QTN,and 19 candidate genes were identified,revealing the genetic mechanisms underlying mucilage secretion under low-nitrogen conditions.These findings provide a comprehensive genetic analysis of the mucilage-secreting ability of maize aerial roots,contributing to our understanding of nitrogen fixation and offering potential avenues for enhancing nitrogen fixation in modern maize lines.This research advances knowledge of plant nutrient acquisition strategies and has implications for sustainable agricultural practices.展开更多
Various forms of nitrogen(N)discharged by high-intensity human activities in the Yangtze River Delta are transported into the lake along the river channel,accelerating the lake’s N cycle and increasing the eutrophica...Various forms of nitrogen(N)discharged by high-intensity human activities in the Yangtze River Delta are transported into the lake along the river channel,accelerating the lake’s N cycle and increasing the eutrophication ecological risk.Taihu Lake is a typical eutrophic shallowlake,suffering fromcyanobacteria blooms for decades due to excessive exogenous nutrient load.In this study,the coupling relationship between basin N loss and lake responsewas established by combining N flow and exogenous nutrient load.The results showed striking spatiotemporal differences and the large tributaries input themajority of N.Three evolution stages of the lake ecosystem were classified,i.e.,Stage A(1980–1997)with slow increasing N load;Stage B(1998–2006)with high-level N load despite some controlling methods;Stage C(2007 to present)with the strengthening of N management in lake basin after the Water Crisis,the N load has gradually decreased,while the water flow is increasing by the year.Environmental N export in the basin was 581.46 kg/ha N in 2021,and a total of 32.06 Gg N was finally drawn into the lake.Over the recent two decades,the noticeable expansion of built-up land from 8.21%to 21.04%associated with its environmental impacts i.e.,urban heat island effect,hard pavement,and ecological fragility deserves attention.Accordingly,the rapid climate change of the basin became the key factor driving the tributaries’hydrologic conditions(r_(∂)=0.945).The developed social economy dominated the sewage discharge(r_(∂)=0.857).The N inputs and losses to the environment in the basin can be further exacerbated without control.Meanwhile,the lake would respond to the exogenous input.In addition to the self-cleaning part of the lake,the N accumulation rate of the surface sediment ranged from 3.29 to 10.77 g N/(m^(2)·yr)of Taihu Lake.To meet the pollutant control target,around 66.28 Gg anthropogenic N needs to be reduced in the upper stream area yearly.Clarifying the N flow and its environmental burden can mitigate its damage to the ecosystem and take on the refined management on the watershed scale.展开更多
The yield of maize(Zea mays L.)is highly influenced by nitrogen fertilization.This study investigated the impact of nitrogen fertilization on morphophysiological traits in maize(Zea mays L.)and developed algorithms to...The yield of maize(Zea mays L.)is highly influenced by nitrogen fertilization.This study investigated the impact of nitrogen fertilization on morphophysiological traits in maize(Zea mays L.)and developed algorithms to relate manual phenotyping and digital phenotyping of maize with leaf nitrogen and digital field image traits.The experiment included three hybrid maize varieties,V1(Hybrid 981),V2(BARI Hybrid maize-9),and V3(Hybrid P3396),which were evaluated across three nitrogen levels(N1=100 kg N ha^(−1),N2=200 kg N ha^(−1),N3=300 kg N ha^(−1))in a split-plot design with three replications.The results revealed that nitrogen levels(N),varieties(V),and their interactions(V×N)significantly influenced traits such as plant height(PH),leaf area index(LAI),normalized difference vegetation index(NDVI),canopy cover(CC),chlorophyll content(Chl a and Chl b),leaf nitrogen content(LNC),total dry matter(TDM),and grain yield.The hybrid P3396 with 300 kg N ha^(−1)(V3N3)achieved the highest grain yield of 14.45 t ha^(−1),which was statistically similar to that of Hybrid 981 and 300 kg N ha^(−1)(V1N3).Nitrogen significantly improved dry matter accumulation,leaf area,and physiological parameters,with maximum values recorded during flowering.The NDVI,CC,and SPAD were strongly correlated with LNC and TDM,highlighting their potential as indicators for nitrogen management.The digital imaging traits analysed via software effectively differentiated the nitrogen treatments,demonstrating their utility for precise nitrogen application.In conclusion,nitrogen fertilization at 300 kg N ha^(−1) optimized the growth and yield of hybrid maize,with Hybrid P3396 performing best.This study underscores the role of advanced phenotyping tools in improving nitrogen use efficiency and sustainable maize production.展开更多
Soil mineralized nitrogen(N)is a vital component of soil N supply capacity and an important N source for rice growth.Unveiling N mineralization(Nm)process characteristics and developing a simple and effective approach...Soil mineralized nitrogen(N)is a vital component of soil N supply capacity and an important N source for rice growth.Unveiling N mineralization(Nm)process characteristics and developing a simple and effective approach to evaluate soil Nm are imperative to guide N fertilizer application and enhance its efficiency in various paddy soils with different physicochemical properties.Soil properties are important driving factors contributing to soil Nm differences and must be considered to achieve effective N management.Nevertheless,discrepancies in Nm capacity and other key influencing factors remain uncertain.To address this knowledge gap,this study collected 52 paddy soil samples from Taihu Lake Basin,China,which possess vastly different physicochemical properties.The samples were subjected to a 112-d submerged anaerobic incubation experiment at a constant temperature to obtain the soil Nm characteristics.Reaction kinetics models,including one-pool exponential model,two-pool exponential model,and effective cumulative temperature model,were employed to compare characteristic differences between Nm potential(Nmp)and short-term accumulated mineralized N(Amn)processes in relation to soil physicochemical properties.Based on these relationships,simplified Nmp prediction methods for paddy soils were established.The results revealed that the Nmp values were 145.18,88.64,and 21.03 mg kg-1 in paddy soils with pH<6.50,6.50≤pH≤7.50,and pH>7.50,respectively.Significantly,short-term Amn at day 14 showed a good correlation(P<0.01)with Nmp(R2=0.94),indicating that the prevailing short-term incubation experiment is an acceptable marker for Nmp.Moreover,Nmp correlated well with the ultraviolet absorbance value at 260 nm based on NaHCO3 extraction(Na260),further streamlining the Nmp estimation method.The incorporation of easily obtainable soil properties,including pH,total N(TN),and the ratio of total organic carbon to TN(C/N),alongside Na260 for Nmp evaluation allowed the multiple regression model,Nmp=58.62×TN-23.18×pH+13.08×C/N+86.96×Na260,to achieve a high prediction accuracy(R2=0.95).The reliability of this prediction was further validated with published data of paddy soils in the same region and other rice regions,demonstrating the regional applicability and prospects of this model.This study underscored the roles of soil properties in Nm characteristics and mechanisms and established a site-specific prediction model based on rapid extractions and edaphic properties of paddy soils,paving the way for developing rapid and precise Nm prediction models.展开更多
The unreasonable application of nitrogen fertilizer poses a threat to agricultural productivity and the environment protection in Northeast China.Therefore,accurately assessing crop nitrogen requirements and optimizin...The unreasonable application of nitrogen fertilizer poses a threat to agricultural productivity and the environment protection in Northeast China.Therefore,accurately assessing crop nitrogen requirements and optimizing fertilization are crucial for sustainable agricultural production.A three-year field experiment was conducted to evaluate the effects of planting density on the critical nitrogen concentration dilution curve(CNDC)for spring maize under drip irrigation and fertilization integration,incorporating two planting densities:D1(60,000 plants ha^(-1))and D2(90,000 plants ha^(-1))and six nitrogen levels:no nitrogen(N0),90(N90),180(N180),270(N270),360(N360),and 450(N450)kg ha^(-1).A Bayesian hierarchical model was used to develop CNDC models based on dry matter(DM)and leaf area index(LAI).The results revealed that the critical nitrogen concentration exhibited a power function relationship with both DM and LAI,while planting density had no significant impact on the CNDC parameters.Based on these findings,we propose unified CNDC equations for maize under drip irrigation and fertilization integration:Nc=4.505DM-0.384(based on DM)and Nc=3.793LAI-0.327(based on LAI).Additionally,the nitrogen nutrition index(NNI),derived from the CNDC,increased with higher nitrogen application rates.The nitrogen nutrition index(NNI)approached 1 with a nitrogen application rate of 180 kg ha^(-1)under the D1 planting density,while it reached 1 at 270 kg ha^(-1)under the D2 planting density.The relationship between NNI and relative yield(RY)followed a“linear+plateau”model,with maximum RY observed when the NNI approached 1.Thus,under the condition of drip irrigation and fertilization integration in Northeast China’s spring maize production,the optimal nitrogen application rates for achieving the highest yields were 180 kg ha^(-1)at a planting density of 60,000 plants ha^(-1),and 270 kg ha^(-1)at a density of 90,000 plants ha^(-1).The CNDC and NNI models developed in this study are valuable tools for diagnosing nitrogen nutrition and guiding precise fertilization practices in maize production under integrated drip irrigation and fertilization systems in Northeast China.展开更多
基金supported by the Xinjiang Outstanding Youth Fund(2021D01E03)the Natural Science Foundation of Xinjiang Uygur Autonomous Region(2022D01D083)the National Natural Science Foundation of China(U2003214,41977099).
文摘In dryland ecosystems,nitrogen(N)is the primary limiting factor after water availability,constraining both plant productivity and organic matter decomposition while also regulating ecosystem function and service provision.However,the distributions of different soil N fraction stocks in drylands and the factors that influence them remain poorly understood.In this study,we collected 2076 soil samples from 173 sites across the drylands of northern China during the summers of 2021 and 2022.Using the best-performing eXtreme Gradient Boosting(XGBoost)model,we mapped the spatial distributions of the soil N fraction stocks and identified the key drivers of their variability.Our findings revealed that the stocks of total nitrogen(TN),inorganic nitrogen(IN),and microbial biomass nitrogen(MBN)in the top 30 cm soil layer were 1020.4,92.2,and 40.8 Tg,respectively,with corresponding mean densities of 164.6,14.9,and 6.6 g/m2.Climate variables-particularly mean annual temperature and aridity-along with human impacts emerged as the dominant drivers of soil N stock distribution.Notably,increased aridity and intensified human impacts exerted mutually counteracting effects on soil N fractions:aridity-driven moisture limitation generally suppressed N accumulation,whereas anthropogenic activities(e.g.,fertilization and grazing)promoted N enrichment.By identifying the key environmental and anthropogenic factors shaping the soil N distribution,this study improves the accuracy of regional and global N stock estimates.These insights provide a scientific foundation for developing more effective soil N management strategies in dryland ecosystems,contributing to sustainable land use and long-term ecosystem resilience in drylands.
基金supported by the National Natural Science Foundation of China for Young Scholars(52109066)the Postdoctoral Science Foundation of Shaanxi Province,China(2023BSHTBZZ29)the China Postdoctoral Science Foundation(2022M712604 and 2023T160534).
文摘Soil nitrogen(N)is the main limiting nutrient for plant growth,which is sensitive to variations in the soil oxygen environment.To provide insights into plant N accumulation and yield under aerated and drip irrigation,a greenhouse tomato experiment was conducted with six treatments,including three fertilization types:inorganic fertilizer(NPK);organic fertilizer(OM);chemical(75%of applied N)+organic fertilizer(25%)(NPK+OM)under drip irrigation(DI)and aerated irrigation(AI)methods.Under Al,total soil carbon mineralization(C_(min))was significantly higher(by 5.7-7.0%)than under DI irrigation.C_(min)in the fertilizer treatments followed the order NPK+OM>OM>NPK under both AI and DI.Potentially mineralizable C(C_(0))and N(N_(0))was greater under AI than under DI.Gross N mineralization,gross nitrification,and NH_(4)^(+)immobilization rates were significantly higher under the AINPK treatment than the DINPK treatment by 2.58-3.27-,1.25-1.44-,and 1-1.26-fold,respectively.These findings demonstrated that AI and the addition of organic fertilizer accelerated the turnover of soil organic matter and N transformation processes,thereby enhancing N availability.Moreover,the combination of AI and organic fertilizer application was found to promote root growth(8.4-10.6%),increase the duration of the period of rapid N accumulation(ΔT),and increase the maximum N accumulation rate(V_(max)),subsequently encouraging aboveground dry matter accumulation.Consequently,the AI treatment yield was significantly greater(by 6.3-12.4%)than under the DI treatment.Further,N partial factor productivity(NPFP)and N harvest index(NHI)were greater under AI than under DI,by 6.3 to 12.4%,and 4.6 to 8.1%,respectively.The rankings of yield and NPFP remained consistent,with NPK+OM>OM>NPK under both AI and DI treatments.These results highlighted the positive impacts of AI and organic fertilizer application on soil N availability,N uptake,and overall crop yield in tomato.The optimal management measure was identified as the AINPK+OM treatment,which led to more efficient N management,better crop growth,higher yield,and more sustainable agricultural practices.
基金supported by Supported by National Key Laboratory of Cotton Bio-breeding and Integrated Utilization(CB2023C07)Xinjiang Autonomous Region"Three Agricultural"Backbone Talent Training Program(2022SNGGNT024)Xinjiang Huyanghe City Science and Technology Program(2023C08).
文摘Nitrogen(N)and phosphorus(P)are mineral nutrients essential for plant growth and development,playing a crucial role throughout the plant life cycle.Cotton,a globally significant textile crop,has a particularly high demand for N fertilizer across its developmental stages.This review explores the effects of adequate or deficient N and P levels on cotton growth phases,focusing on their influence on physiological processes and molecular mechanisms.Key topics include the regulation of N-and P-related enzymes,hormones,and genes,as well as the complex interplay of N-and P-related signaling pathways from the aspects of N-P signaling integration to regulate root development,N-P signaling integration to regulate nutrient uptake,and regulation of N-P interactions—a frontier in current research.Strategies for improving N and P use efficiency are also discussed,including developing high-efficiency cotton cultivars and identifying functional genes to enhance productivity.Generally speaking,we take model plants as a reference in the hope of coming up with new strategies for the efficient utilization of N and P in cotton.
基金Forest Ecosystem Research of Liangshui & Maorshan Station of Heilongjiang Province (CFERN, No. 2001-02).
文摘Nitrogen is one of the most important elements that can limit plant growth in forest ecosystems. Studies of nitrogen mineralization, nitrogen saturation and nitrogen cycle in forest ecosystems is very necessary for understanding the productivity of stand, nutrient cycle and turnover of nitrogen of forest ecosystems. Based on comparison and analysis of domestic and in-ternational academic references related to studies on nitrogen mineralization, nitrogen saturation and nitrogen cycle in recent 10 years, the current situation and development of the study on these aspects, and the problems existed in current researches were reviewed. At last, some advices were given for future researches.
基金supported by the Natural Science Fund of China(31771724)the Key Research and Development Project of Shaanxi Province(2024NC-ZDCYL-01-10).
文摘The increase in soil temperature associated with climate change has introduced considerable challenges to crop production.Split nitrogen application(SN)represents a potential strategy for improving crop nitrogen use efficiency and enhancing crop stress resistance.Nevertheless,the precise interaction between soil warming(SW)and SN remains unclear.In order to ascertain the impact of SW on maize growth and whether SN can improve the tolerance of maize to SW,a two-year field experiment was conducted(2022-2023).The aim was to examine the influence of two SW ranges(MT,warming 1.40℃;HT,warming 2.75℃)and two nitrogen application methods(N1,one-time basal application of nitrogen fertilizer;N2,one third of base nitrogen fertilizer+two thirds of jointing stage supplemental nitrogen fertilizer)on maize root growth,photosynthetic characteristics,nitrogen use efficiency,and yield.The results demonstrated that SW impeded root growth and precipitated the premature aging of maize leaves following anthesis,particularly in the HT,which led to a notable reduction in maize yield.In comparison to N1,SN has been shown to increase root length density by 8.54%,root bleeding rate by 8.57%,and enhance root distribution ratio in the middle soil layers(20-60 cm).The interaction between SW and SN had a notable impact on maize growth and yield.The SN improved the absorption and utilization efficiency of nitrogen by promoting root development and downward canopy growth,thus improving the tolerance of maize to SW at the later stage of growth.In particular,the N2HT resulted in a 14.51%increase in the photosynthetic rate,a 18.58%increase in nitrogen absorption efficiency,and a 18.32%increase in maize yield compared with N1HT.It can be posited that the SN represents a viable nitrogen management measure with the potential to enhance maize tolerance to soil high-temperature stress.
基金Supported by National Natural Science Foundation of China(41371259)Hubei Natural Science Foundation(2014CFB545)~~
文摘The soybean, cotton, maize and sorghum were planted in pot under low nitrogen, high nitrogen treatments, the soil available nitrogen constitution and con- version and utilization of nitrogen fertilizer were determined, so as to provide techni- cal guidance for reasonable use and improving use efficiency of nitrogen fertilizer for different types of crops. Compared with the control with nitrogen but unplanted crop, growing soybean, cotton, maize, sorghum significantly decreased the soil available N contents by 53. 48%, 51.54%, 33.10%, 55.03%,and influenced the constitution of soil available N. Thereinto, growing soybean, cotton, maize and sorghum significantly decreased soil inorganic N contents by 85.41%, 83.09%, 70.89% and 83.35%,but increased soil hydrolysable organic N contents by 1.41, 1.53, 2.11 and 1.28 times, respectively; growing soybean, cotton, maize and sorghum significantly decreased the rate of soil inorganic N to available N by 68.61%, 65.09%, 56.47% and 63.00%, but increased the rate of soil hydrolysable organic N to available N by 4.18, 4.21, 3.66 and 4.08 times, respectively. Compared with the control, growing soybean, cotton, maize and sorghum significantly increased the transform rate of ammonium nitrogen fertilizer by 93.66%, 38.19%, 32.58% and 38.31% respectively, and growing soybean treatment had the highest increasing range; the nitrification rates of ammo- nium nitrogen fertilizer of growing soybean, cotton, maize and sorghum treatments were negative values, and growing soybean treatment had the highest decreasing amplitude. The ammonium nitrogen fertilizer use efficiency of growing soybean, cot- ton, maize and sorghum treatments were 52.01%, 28.31%, 24.16% and 28.40% re- spectively and growing soybean treatment had the highest value. In conclusion, growing crops suppressed the soil nitrification and accelerated the development of soil hydrolysable organic nitrogen by the utilization of soil available nitrogen and the alteration of soil environment, and hence impacted the constitution of soil available nitrogen and the transform and use of ammonium nitrogen applied in soil. Legumi- nous crops had stronger ability of suppressing nitrification, making use of ammonium compared with non-Leguminous crops.
基金supported by the National Natural Science Foundation of China(51609247)the Henan Provincial Natural Science Foundation,China(222300420589,202300410553)+4 种基金the Central Public-interest Scientific Institution Basal Research Fund,China(FIRI2022-22)the Science&Technology Fundamental Resources Investigation Program,China(2022FY101601)the Science and Technology Project of Xinxiang City,Henan Province,China(GG2021024)the Major Special Science and Technology Project of Henan Province,China(221100110700)the Joint Fund of Science and Technology Research and Development Plan of Henan Province,China(Superior Discipline Cultivation)(222301420104)。
文摘Nitrogen(N)uptake is regulated by water availability,and a water deficit can limit crop N responses by reducing N uptake and utilization.The complex and multifaceted interplay between water availability and the crop N response makes it difficult to predict and quantify the effect of water deficit on crop N status.The nitrogen nutrition index(NNI)has been widely used to accurately diagnose crop N status and to evaluate the effectiveness of N application.The decline of NNI under water-limiting conditions has been documented,although the underlying mechanism governing this decline is not fully understood.This study aimed to elucidate the reason for the decline of NNI under waterlimiting conditions and to provide insights into the accurate utilization of NNI for assessing crop N status under different water-N interaction treatments.Rainout shelter experiments were conducted over three growing seasons from 2018 to 2021 under different N(75 and 225 kg N ha^(-1),low N and high N)and water(120 to 510 mm,W0 to W3)co-limitation treatments.Plant N accumulation,shoot biomass(SB),plant N concentration(%N),soil nitrate-N content,actual evapotranspiration(ET_a),and yield were recorded at the stem elongation,booting,anthesis and grain filling stages.Compared to W0,W1 to W3 treatments exhibited NNI values that were greater by 10.2 to 20.5%,12.6to 24.8%,14 to 24.8%,and 16.8 to 24.8%at stem elongation,booting,anthesis,and grain filling,respectively,across the 2018-2021 seasons.This decline in NNI under water-limiting conditions stemmed from two main factors.First,reduced ET_(a) and SB led to a greater critical N concentration(%N_(c))under water-limiting conditions,which contributed to the decline in NNI primarily under high N conditions.Second,changes in plant%N played a more significant role under low N conditions.Plant N accumulation exhibited a positive allometric relationship with SB and a negative relationship with soil nitrate-N content under water-limiting conditions,indicating co-regulation by SB and the soil nitrate-N content.However,this regulation was influenced by water availability.Plant N accumulation sourced from the soil nitrate-N content reflects soil N availability.Greater soil water availability facilitated greater absorption of soil nitrate-N into the plants,leading to a positive correlation between plant N accumulation and ET_(a)across the different water-N interaction treatments.Therefore,considering the impact of soil water availability is crucial when assessing soil N availability under water-limiting conditions.The findings of this study provide valuable insights into the factors contributing to the decline in NNI among different water-N interaction treatments and can contribute to the more accurate utilization of NNI for assessing winter wheat N status.
基金Supported by Natural Science Foundation of Guangxi Province(0728062)Natural Science Foundation of Guangxi Province(0832008Z)~~
文摘[Objective]The paper was to study the effect of different nitrogen application levels and modes on contents of different forms of nitrogen in rice plants.[Method] Using Guichao 2 and Diejiazhan as test materials,with total nitrogen,protein nitrogen,chlorophyll nitrogen,soluble protein nitrogen,Rubisco nitrogen,free amino acid nitrogen as indicators,the effect of nitrogen application on contents of different forms of nitrogen in rice plants were studied.[Result] The contents of total nitrogen,protein nitrogen,chlorophyll nitrogen,soluble protein nitrogen,Rubisco-N,free amino nitrogen of rice plant during heading and maturity stage increased with the increasing application amount of nitrogen,and their contents would be high under heavy application of spike fertilizer when the application amount of nitrogen was the same.However,application of nitrogen had different impact on different forms of nitrogen,which showed obvious impact on the contents of Rubisco-N and free amino nitrogen.The differences of Rubisco-N and free amino nitrogen content of two varieties during heading and maturity stage under different nitrogen application modes all reached significant or extremely significant level.Correlation analysis showed that total nitrogen content of rice plant had extremely significant correlation with protein nitrogen content during heading and maturity stage in both early and late seasons.[Conclusion] The study provided a theoretical basis for clarifying the regulatory role of nitrogen application on nitrogen absorption and utilization.
基金supported by grants from Hainan Seed Laboratory(B21HJ0003)the National Natural Science Foundation of China(U23A20185)the Hainan Excellent Talent Team。
文摘Light and nitrogen(N)are two critically environmental factors essential for plant survival,as they constitute the fundamental molecular framework of plant cells and significantly influence patterns of plant growth and development.Light is the driving force behind photosynthesis,a process that converts light energy into chemical energy stored as sugars.Additionally,light acts as a direct signal that can modulate plant morphogenesis and structural development.Nitrogen,as the most crucial mineral nutrient for plants,is a component of numerous biomolecules.It also functions as a signaling molecule,regulating plant growth and development.Moreover,light and nitrogen directly regulate the balance of carbon(C)and N within plants,affecting numerous biochemical reactions and various physiological processes.This review focuses on the interactions between light and nitrogen in physiological,metabolic,and molecular levels.We will also discuss the regulatory networks and mechanisms through which light and nitrogen influence C and N absorption and metabolism in plants.
基金the Natural Science Foundation of Jiangsu Province,China(BK20200539)the Jiangsu Provincial Key Research and Development Program,China(BE2019377 and BE2019343)the Science and Technology Planning Program of Suzhou,China(ST202228).
文摘Synchronizing the nitrogen(N)supply of slow-and controlled-release N fertilizers(SCRNFs)with rice N demand is essential in replacing multiple urea applications with a single basal application of SCRNFs.Traditional assessment of N supply characteristics primarily examines N release patterns,which are limited to coated SCRNFs and disregard N transformation mechanisms,necessitating a more universal and reliable index.Based on the capacity of crop N status to detect N deficiency or excess,we hypothesized that utilizing leaf N balance index(NBI)as a measure of N status could offer novel insights into assessing N supply characteristics of SCRNFs.Field experiments were conducted with four individual SCRNFs-humic acid urea(HAU),sulfur-coated urea(SCU),urease inhibitor urea(UIU),and polymer-coated urea(PCU)and their four combined forms,alongside high-yield urea split application as control(CK).The results revealed that NBI dynamics relative to CK reflected the N supply potential of different SCRNFs while categorizing them as short-,medium-,and long-acting fertilizers.Combinations incorporating the long-acting SCRNF(PCU)consistently demonstrated superior performance in yield(by 5.5%)and N use efficiency(by 42.8%)through providing more consistent and efficient N supply throughout the rice growth cycle.Grain yield exhibited negative correlation with the difference in NBI dynamics between SCRNFs and CK,suggesting that synchronizing N supply between one-time application of SCRNFs and conventional high-yield fertilization is crucial for high yield.These findings demonstrate the potential of N status diagnosed by leaf NBI to evaluate N supply characteristics of SCRNFs and highlight the importance of synchronized N supply for a one-time SCRNF application.
基金supported by the National Natural Science Foundation of China(31860345 and 31460541)the Youth Innovative Top Talents Project of Shihezi University,China(CXBJ202003)the Third Division of Xinjiang Production and Construction Corps Scientific and Technological Achievements Transfer and Transformation Project,China(KJ2023CG03)。
文摘The responses of drip-irrigated rice physiological traits to water and fertilizers have been widely studied.However,the responses of yield,root traits and their plasticity to the nitrogen environment in different nitrogen-efficient cultivars are not fully understood.An experiment was conducted from 2020-2022 with a high nitrogen use efficiency(high-NUE)cultivar(T-43)and a low-NUE cultivar(LX-3),and four nitrogen levels(0,150,300,and 450 kg ha^(-1))under drip irrigation in large fields.The aim was to study the relationships between root morphology,conformation,biomass,and endogenous hormone contents,yield and NUE.The results showed three main points:1)Under the same N application rate,compared with LX-3,the yield,N partial factor productivity(PFP),fine root length density(FRLD),shoot dry weight(SDW),root indole-3-acetic acid(IAA),and root zeatin and zeatin riboside(Z+ZR)of T-43 were significantly greater by11.4-18.9,11.3-13.5,11.6-15.7,9.9-31.1,6.1-48.1,and 22.8-73.6%,respectively,while the root-shoot ratio(RSR)and root abscisic acid(ABA)were significantly lower(P<0.05);2)nitrogen treatment significantly increased the rice root morphological indexes and endogenous hormone contents(P<0.05).Compared to N0,the yield,RLD,surface area density(SAD),root volume density(RVD),and root endogenous hormones(IAA,Z+ZR)were significantly increased in both cultivars under N2 by 61.6-71.6,64.2-74.0,69.9-105.6,6.67-9.91,54.0-67.8,and 51.4-58.9%,respectively.Compared with N3,the PFP and N agronomic efficiency(NAE)of nitrogen fertilizer under N2 increased by 52.3-62.4 and39.2-63.0%,respectively;3)the responses of root trait plasticity to the N environment significantly differed between the cultivars(P<0.05).Compared with LX-3,T-43 showed a longer root length and larger specific surface area,which is a strategy for adapting to changes in the nutrient environment.For the rice cultivar with high-NUE,the RSR was optimized by increasing the FRLD,root distribution in upper soil layers,and root endogenous hormones(IAA,Z+ZR)under suitable nitrogen conditions(N2).An efficient nutrient acquisition strategy can occur through root plasticity,leading to greater yield and NUE.
基金supported by the National Key Research and Development Program of China(2023YFD2301702)the earmarked Fund for China Agriculture Research System(CARS-02)the National Natural Science Foundation of China(31971852).
文摘Long-term excessive nitrogen(N)application neither increases nor enhances grain yield and N use efficiency(NUE)of maize,yet the mechanisms involving root morphological and physiological characteristics remain unclear.This study aimed to elucidate the mechanisms underlying stagnant grain yield under excessive N application by examining root morphological and physiological characteristics.A 10-year N fertilizer trial was conducted in Jilin Province,Northeast China,cultivating maize at three N fertilizer levels(zero N,N0;recommended N,N2;and high N level,N4)from 2019 to 2021.Two widely cultivated maize genotypes,‘Xianyu 335’(XY335)and‘Zhengdan 958’(ZD958),were evaluated.Grain yield,N content,root morphology,and physiological characteristics were analyzed to assess the relationships between N uptake,N utilization,plant growth,and root systems under different N treatments.Compared to N0,root biomass,post-silking N uptake,and grain yield improved significantly with increased N input,while no significant differences emerged between recommended N and high N.High N application enhanced root length and root surface area but decreased root activity(measured by TTC(2,3,5-triphenyltetrazolium chloride)method),nitrate reductase activity,and root activity absorbing area across genotypes.Root length and root to shoot ratio negatively affected N uptake(by-1.2 and-24.6%),while root surface area,root activity,nitrate reductase activity,and root activity absorbing area contributed positively.The interaction between cultivar and N application significantly influenced NUE.XY335 achieved the highest NUE(11.6%)and N recovery efficiency(18.4%)through superior root surface area(23.6%),root activity(12.5%),nitrate reductase activity(8.3%),and root activity absorbing area(6.9%)compared to other treatments.Recommended N application enhanced Post N uptake,NUE,and grain yield through improved root characteristics,while high N application failed to increase or decreased NUE by reducing these parameters.This study demonstrates that root surface area,root activity,nitrate reductase activity,and root activity absorbing area limit NUE increase under high N application.
基金supported by the National Natural Science Foundation of China(No.32160142)Guangxi Natural Science Foundation(No.2023GXNSFDA026034)+3 种基金State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources(SKLCUSAb202302)to H.W.,the National Natural Science Foundation of China(No.32460062)to Y.L.,and 1+9 Leading the Charge with Open Competition'project of Sichuan Academy of Agricultural Sciences(1+9KJGG010)Fruit tree breeding project in Sichuan Province(2021YFYZ0023)to H.X.
文摘Symbiotic nitrogen fixation in members of the Fabaceae family is highly efficient and beneficial for global agriculture,but not all species in this family form root nodules with rhizobial bacteria.Nodulation mainly occurs in plants belonging to the Papilionoideae and Caesalpinioideae subfamilies(Tederso0 et al.,2018;van Velzen et al.,2019).Nodulation mechanisms in Fabaceae are well studied(Yang et al.,2022),and genomic comparisons of nodulating and non-nodulating host species can provide valuable insights into the evolutionary and genetic basis of this key process.
基金financially supported by the National Natural Science Foundation of China(No.32172109)the Natural Science Foundation of Guangdong Province,China(No.2021A1515010566).
文摘Nitrogen(N)is the most important nutrient for plants;however,microbe-mediated N transformation under different N forms is unclear.This experiment investigated the effects of four treatments fertilized with various N forms,no N(control,CK),100%ammonium N(AN),100%nitrate N(NN),and 50%ammonium N+50%nitrate N(ANNN),on soil chemical properties,rhizosphere bacterial network,and rice growth.The ANNN treatment enhanced soil pH by 6.9%,soil organic carbon by 12%,and microbial biomass N(MBN)by 60%compared to CK.The linear discriminant effect size(LEfSe)analysis indicated four highly abundant biomarkers of bacterial communities each in the CK,NN,and AN treatments,while the ANNN treatment showed six highly abundant biomarkers with maximum effect size and linear discriminant analysis(LDA)score>4.The 16S rRNA gene-predicted functions under PICRUST indicated glutathione metabolism and proteasome and Tax4Fun recorded amino acid metabolism in the ANNN treatment.The combination of ammonium and nitrate N(i.e.,the ANNN treatment)significantly increased the expression levels of the genes encoding N metabolism,including AMT1,NRT2.1,GS1,and GOGAT1,and induced 39%,27%,35%,and 38%increase in nitrate reductase,nitrite reductase,glutamine synthetase,and glutamate synthase,respectively,in comparison to CK.In addition,the ANNN treatment promoted rice leaf photosynthetic rate by 37%,transpiration rate by 41%,CO_(2) exchange rate by 11%,and stomatal conductance by 18%compared to CK,while increased N use efficiency(NUE)by 10%and 19%,respectively,compared to the AN and NN treatments.These findings suggest that the combination of ammonium and nitrate N can promote bacterial community abundance,composition,and functional pathways by improving soil properties and can increase NUE and rice growth.This study provides a theoretical basis for the rational application of N fertilizers and the implications of this approach for future sustainable crop production.
基金supported by the CASHIPS Director’s Fund (Grant No. YZJJ202207-CX)。
文摘Polymeric nitrogen is a potential high-energy-density material with the advantages of high energy density, easy availability of raw materials, and non-pollution. The design and synthesis of polymeric nitrogen are important in the research field of energetic materials. The cubic gauche nitrogen was successfully synthesized at high pressure in the diamond anvil cell, which stimulated the theoretical and experimental investigations. To date, several hundred kinds of polymeric nitrogen have been reported. This review introduces the progressive development of polymeric nitrogen with high energy density, the challenges faced by the synthesized polymeric nitrogen under high-pressure,and the importance to improve the stability of polymeric nitrogen at ambient pressure. Furthermore, alternative methods for synthesizing polymeric nitrogen under moderate conditions are also presented. In this field, more efforts are needed to develop strategies for stabilizing more polymeric nitrogen to ambient conditions, especially the stability of free surfaces.
基金supported by the National Natural Science Foundation of China(32401919)the Department of Science and Technology of Henan Province(242102111126).
文摘Recent studies have shown that mucilage secretion from aerial roots is an essential feature of modern maize inbred lines,with some retaining the nitrogen-fixing capabilities of ancient landraces.To explore the genetic basis of nitrogen fixation in mucilage and its evolution from teosinte(Zea mays ssp.mexicana)to modern maize,we developed a recombinant inbred line(RIL)population from teosinte and cultivated it under low-nitrogen conditions.Large-scale,multi-year,and multi-environment analyses of RIL-Teo,Doubled Haploid-A(DH-A),Doubled Haploid-B(DH-B),and association populations led to the identification of 15 quantitative trait loci(QTL),68 quantitative trait nucleotides(QTN),and 59 candidate genes linked to mucilage secretion from aerial roots.Functional verification of the candidate gene ZmAco3,which is associated with mucilage secretion in aerial roots,demonstrated that deletion of this gene resulted in a reduction in mucilage secretion in aerial roots.In addition,most maize inbred lines exhibited stronger mucilage secretion from aerial roots under low-nitrogen conditions than under normal-nitrogen conditions.We categorized mucilage secretion into constitutive and low-nitrogen-inducible types.Through genotype-by-environment interaction studies,8 QTL,16 QTN,and 19 candidate genes were identified,revealing the genetic mechanisms underlying mucilage secretion under low-nitrogen conditions.These findings provide a comprehensive genetic analysis of the mucilage-secreting ability of maize aerial roots,contributing to our understanding of nitrogen fixation and offering potential avenues for enhancing nitrogen fixation in modern maize lines.This research advances knowledge of plant nutrient acquisition strategies and has implications for sustainable agricultural practices.
基金supported by the National Key Research and Development Program of China(No.2021YFC3201502)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.KYCX24_1830).
文摘Various forms of nitrogen(N)discharged by high-intensity human activities in the Yangtze River Delta are transported into the lake along the river channel,accelerating the lake’s N cycle and increasing the eutrophication ecological risk.Taihu Lake is a typical eutrophic shallowlake,suffering fromcyanobacteria blooms for decades due to excessive exogenous nutrient load.In this study,the coupling relationship between basin N loss and lake responsewas established by combining N flow and exogenous nutrient load.The results showed striking spatiotemporal differences and the large tributaries input themajority of N.Three evolution stages of the lake ecosystem were classified,i.e.,Stage A(1980–1997)with slow increasing N load;Stage B(1998–2006)with high-level N load despite some controlling methods;Stage C(2007 to present)with the strengthening of N management in lake basin after the Water Crisis,the N load has gradually decreased,while the water flow is increasing by the year.Environmental N export in the basin was 581.46 kg/ha N in 2021,and a total of 32.06 Gg N was finally drawn into the lake.Over the recent two decades,the noticeable expansion of built-up land from 8.21%to 21.04%associated with its environmental impacts i.e.,urban heat island effect,hard pavement,and ecological fragility deserves attention.Accordingly,the rapid climate change of the basin became the key factor driving the tributaries’hydrologic conditions(r_(∂)=0.945).The developed social economy dominated the sewage discharge(r_(∂)=0.857).The N inputs and losses to the environment in the basin can be further exacerbated without control.Meanwhile,the lake would respond to the exogenous input.In addition to the self-cleaning part of the lake,the N accumulation rate of the surface sediment ranged from 3.29 to 10.77 g N/(m^(2)·yr)of Taihu Lake.To meet the pollutant control target,around 66.28 Gg anthropogenic N needs to be reduced in the upper stream area yearly.Clarifying the N flow and its environmental burden can mitigate its damage to the ecosystem and take on the refined management on the watershed scale.
基金supported by the Bangladesh Agricultural Research Insti-tute,Gazipur-1701,Bangladesh.This research was also funded by Taif University,Saudi Arabia,Project No.(TU-DSPP-2024-07).
文摘The yield of maize(Zea mays L.)is highly influenced by nitrogen fertilization.This study investigated the impact of nitrogen fertilization on morphophysiological traits in maize(Zea mays L.)and developed algorithms to relate manual phenotyping and digital phenotyping of maize with leaf nitrogen and digital field image traits.The experiment included three hybrid maize varieties,V1(Hybrid 981),V2(BARI Hybrid maize-9),and V3(Hybrid P3396),which were evaluated across three nitrogen levels(N1=100 kg N ha^(−1),N2=200 kg N ha^(−1),N3=300 kg N ha^(−1))in a split-plot design with three replications.The results revealed that nitrogen levels(N),varieties(V),and their interactions(V×N)significantly influenced traits such as plant height(PH),leaf area index(LAI),normalized difference vegetation index(NDVI),canopy cover(CC),chlorophyll content(Chl a and Chl b),leaf nitrogen content(LNC),total dry matter(TDM),and grain yield.The hybrid P3396 with 300 kg N ha^(−1)(V3N3)achieved the highest grain yield of 14.45 t ha^(−1),which was statistically similar to that of Hybrid 981 and 300 kg N ha^(−1)(V1N3).Nitrogen significantly improved dry matter accumulation,leaf area,and physiological parameters,with maximum values recorded during flowering.The NDVI,CC,and SPAD were strongly correlated with LNC and TDM,highlighting their potential as indicators for nitrogen management.The digital imaging traits analysed via software effectively differentiated the nitrogen treatments,demonstrating their utility for precise nitrogen application.In conclusion,nitrogen fertilization at 300 kg N ha^(−1) optimized the growth and yield of hybrid maize,with Hybrid P3396 performing best.This study underscores the role of advanced phenotyping tools in improving nitrogen use efficiency and sustainable maize production.
基金supported by the Youth Innovation Promotion Association of Chinese Academy of Sciences(No.Y201956)the Young Elite Scientists Sponsorship Program by China Association for Science and Technology(No.2023QNRC001)the National Key Research and Development Program of China(No.2017YFD200104).
文摘Soil mineralized nitrogen(N)is a vital component of soil N supply capacity and an important N source for rice growth.Unveiling N mineralization(Nm)process characteristics and developing a simple and effective approach to evaluate soil Nm are imperative to guide N fertilizer application and enhance its efficiency in various paddy soils with different physicochemical properties.Soil properties are important driving factors contributing to soil Nm differences and must be considered to achieve effective N management.Nevertheless,discrepancies in Nm capacity and other key influencing factors remain uncertain.To address this knowledge gap,this study collected 52 paddy soil samples from Taihu Lake Basin,China,which possess vastly different physicochemical properties.The samples were subjected to a 112-d submerged anaerobic incubation experiment at a constant temperature to obtain the soil Nm characteristics.Reaction kinetics models,including one-pool exponential model,two-pool exponential model,and effective cumulative temperature model,were employed to compare characteristic differences between Nm potential(Nmp)and short-term accumulated mineralized N(Amn)processes in relation to soil physicochemical properties.Based on these relationships,simplified Nmp prediction methods for paddy soils were established.The results revealed that the Nmp values were 145.18,88.64,and 21.03 mg kg-1 in paddy soils with pH<6.50,6.50≤pH≤7.50,and pH>7.50,respectively.Significantly,short-term Amn at day 14 showed a good correlation(P<0.01)with Nmp(R2=0.94),indicating that the prevailing short-term incubation experiment is an acceptable marker for Nmp.Moreover,Nmp correlated well with the ultraviolet absorbance value at 260 nm based on NaHCO3 extraction(Na260),further streamlining the Nmp estimation method.The incorporation of easily obtainable soil properties,including pH,total N(TN),and the ratio of total organic carbon to TN(C/N),alongside Na260 for Nmp evaluation allowed the multiple regression model,Nmp=58.62×TN-23.18×pH+13.08×C/N+86.96×Na260,to achieve a high prediction accuracy(R2=0.95).The reliability of this prediction was further validated with published data of paddy soils in the same region and other rice regions,demonstrating the regional applicability and prospects of this model.This study underscored the roles of soil properties in Nm characteristics and mechanisms and established a site-specific prediction model based on rapid extractions and edaphic properties of paddy soils,paving the way for developing rapid and precise Nm prediction models.
基金supported by the grants from National Key Research and Development Program of China(2023YFD2303300)China Agriculture Research System(CARS-02-15)the Agricultural Science and Technology Innovation Program(CAAS-ZDRW202004).
文摘The unreasonable application of nitrogen fertilizer poses a threat to agricultural productivity and the environment protection in Northeast China.Therefore,accurately assessing crop nitrogen requirements and optimizing fertilization are crucial for sustainable agricultural production.A three-year field experiment was conducted to evaluate the effects of planting density on the critical nitrogen concentration dilution curve(CNDC)for spring maize under drip irrigation and fertilization integration,incorporating two planting densities:D1(60,000 plants ha^(-1))and D2(90,000 plants ha^(-1))and six nitrogen levels:no nitrogen(N0),90(N90),180(N180),270(N270),360(N360),and 450(N450)kg ha^(-1).A Bayesian hierarchical model was used to develop CNDC models based on dry matter(DM)and leaf area index(LAI).The results revealed that the critical nitrogen concentration exhibited a power function relationship with both DM and LAI,while planting density had no significant impact on the CNDC parameters.Based on these findings,we propose unified CNDC equations for maize under drip irrigation and fertilization integration:Nc=4.505DM-0.384(based on DM)and Nc=3.793LAI-0.327(based on LAI).Additionally,the nitrogen nutrition index(NNI),derived from the CNDC,increased with higher nitrogen application rates.The nitrogen nutrition index(NNI)approached 1 with a nitrogen application rate of 180 kg ha^(-1)under the D1 planting density,while it reached 1 at 270 kg ha^(-1)under the D2 planting density.The relationship between NNI and relative yield(RY)followed a“linear+plateau”model,with maximum RY observed when the NNI approached 1.Thus,under the condition of drip irrigation and fertilization integration in Northeast China’s spring maize production,the optimal nitrogen application rates for achieving the highest yields were 180 kg ha^(-1)at a planting density of 60,000 plants ha^(-1),and 270 kg ha^(-1)at a density of 90,000 plants ha^(-1).The CNDC and NNI models developed in this study are valuable tools for diagnosing nitrogen nutrition and guiding precise fertilization practices in maize production under integrated drip irrigation and fertilization systems in Northeast China.
