OBJECTIVE To explore hypoglycemic effect of 95%ethanol fraction of Nitraria roborowskii Kom(NRK-C)and its possible mechanism evaluated in the type 2 diabetes mellitus(T2DM)mice.METHODS The body weight,organ indices,bl...OBJECTIVE To explore hypoglycemic effect of 95%ethanol fraction of Nitraria roborowskii Kom(NRK-C)and its possible mechanism evaluated in the type 2 diabetes mellitus(T2DM)mice.METHODS The body weight,organ indices,blood glucose levels,serum biochemical indexes,as well as HE/PAS histopathological section were all analyzed to assess the hypoglycemic effect of NRK-C in T2DM mice induced by a high-fat diet(HFD)combined with six intraperitoneal injections of 35 mg·kg^(-1)of streptozotocin(STZ).The Western blotting and immunofluorescence were further applied to determine the regulatory effect of NRK-C on key signaling proteins.RESULTS The fasting blood glucose levels were significantly reduced after 7 weeks of administration of NRK-C.In addition,NRK-C could also significantly improve glucose tolerance,hepatic glycogen levels,and lipid levels(total cholesterol,triglyceride,low density lipoprotein and high density lipoprotein),and significantly reduced insulin resistance of diabetic mice,which played an important role in the antidiabetic effects.Further mechanism research demonstrated that phosphorylated PI3K expression was up-regulated and p-GSK3βexpression was up-regulated after NRK-C intervention,indicating that NRK-C might exert a potential antidiabetic effect by modulating the PI3K/AKT signaling pathway.CONCLUSION All these results suggested that NRK-C might improve T2DM and had the potential to be used as an adjunctive therapy.展开更多
Salt stress is a major abiotic stress limiting plant growth and yield. In the present study, the effects of exogenous H_(2)O_(2) on the reactive oxygen species(ROS) metabolism and the antioxidant system in leaves of N...Salt stress is a major abiotic stress limiting plant growth and yield. In the present study, the effects of exogenous H_(2)O_(2) on the reactive oxygen species(ROS) metabolism and the antioxidant system in leaves of Nitralia tangutorum Bobr. under salt stress were studied. N. tangutorum seedlings were subjected to 200 mmol·L^(-1) NaCl treatment with or without the exogenous application of H_(2)O_(2) for 7 days. The results showed that NaCl stress significantly increased the relative conductivity, the contents of thiobarbituric acid reactive substances(TBARS) and ROS(H_(2)O_(2) and O_(2)^(·-)), as well as promoted the activities of antioxidant enzymes including superoxide dismutase(SOD), peroxidase(POD), catalase(CAT), and ascorbate peroxidase(APX) in N. tangutorum leaves. In addition, exogenous H_(2)O_(2) decreased the relative conductivity, the contents of TBARS, H_(2)O_(2) and O_(2)^(·-), while further enhanced the activities of antioxidant enzymes. These results indicated that H_(2)O_(2) effectively alleviated the adverse effects of NaCl stress on N. tangutorum through the regulation of ROS metabolism.展开更多
In this paper,six important distribution areas of Nitraria tangutorum Bobr.in Tsaidam Basin were selected as research objects,to study spatial variability and distribution of soil nutrients in different N.tangutorum p...In this paper,six important distribution areas of Nitraria tangutorum Bobr.in Tsaidam Basin were selected as research objects,to study spatial variability and distribution of soil nutrients in different N.tangutorum populations and analyze the relationship between soil nutrient contents and geographical location,by measuring soil pH and the contents of organic matter（OM）,total nitrogen（N）,total phosphorus（P）,total potassium（K）,hydrolysis N,available P and available K in soils.Results showed that：（1） soil nutrient contents among different populations showed significant spatial variability,and soil depth had a significant effect on soil nutrients contents,but the variation rules were not obvious.（2）Average pH and average contents of OM,total N,total P,total K,hydrolysis N,available P and available K in soils with different depths（0-15,15-30,30-45 cm） varied in the range of 8.37-9.21,3.34-20.68,0.18-1.21,0.35-0.75,16.12-22.04,5.13-553.28,1.10-52.54 and 103.83-562.28 mg/kg,respectively.（3） The analysis results of correlation between average values of pH and contents of nutrient indexes in soils with different depths（0-15,15-30,30-45 cm） showed that the correlation of these indexes were different.（4）OM and total N contents in soils with different depths（0-15,15-30,30-45 cm） all had a significant positive correlation with latitude and negative correlation with longitude and altitude,and the correlation of available P and available K contents in surface soils（0-15 cm） with latitude,longitude and altitude were significant positive,significant negative and significant negative,respectively;moreover,longitude and latitude also showed a significant impact on soil available K contents with the depth of 30-45 cm.In addition,comprehensive analysis result of nutrient contents showed that N.tangutorum populations in Huaitou Tala Town had the highest fertility,and the fertility levels of N.tangutorum populations in Chaka Town and Wulan Keke Town were relatively lower.展开更多
Abstract： Soil water repellency （WR） is an important physical characteristic of soil surface. It is capable of largely influencing the hydrological and geomorphological processes of soil, as well as affecting the e...Abstract： Soil water repellency （WR） is an important physical characteristic of soil surface. It is capable of largely influencing the hydrological and geomorphological processes of soil, as well as affecting the ecological processes of plants, such as growth and seed germination, and has thus been a hot topic in recent research around the world. In this paper, the capillary rise method was used to study the soil WR characteristics of Nitraria tangutorun nebkhas. Soil water repellencies at different succession stages of Nitraria tangutorun were investigated, and the relationships between soil WR and soil organic matter, total N, and total P, soil texture, pH, and concentrations of CO32, HCO3-, CI, SO42-, Na~, K~, Ca2~ and Mg2＋ were discussed. Soil WR may be demonstrated at the following nebkhas dune evolvement stages： extremely degraded〉degraded〉stabilized〉well developed〉newly developed〉quick sand. Apart from some soil at the bottom, the WR of other soils （crest and slope of dune） was found to be largest at the topsoil, and decreased as the soil depth increased. The results showed that multiple factors affected soil WR characteristics e.g. WR increased significantly as the contents of soil organic matter and total N increased, but did not change as the total P content increased. Soil texture was a key factor affecting soil WR; soil WR increased significantly as clay content increased, and decreased significantly as sand content increased. Low pH was shown to be more suitable for the occurrence of soil WR. Four cations （Ca2＋, Mg2＋, K＋ and Na＋） and two anions （CI and SO42） enhanced soil WR, while CO32-decreased it. HCO3- did not show any observable effect. Finally, we established a best-fit general linear model （GLM） between soil-air-water contact angle （CA） and influencing factors （CA=5.606 sand＋6.496 （clay and silt）-2.353 pH＋470.089 CQ2＋11.346 Na＋-407.707 Cl--14.245 SO42-＋0.734 total N-519.521 ）. It was concluded that all soils contain subcritical WR （0°〈CA〈90°）. The development and succession of Nitraria tangutorun nebkhas may improve the formation of soil subcritical WR. There exist significant relationships between soils subcritical WR and soil physical or chemical properties.展开更多
In traditional Chinese medicine, Nitraria sibirica Pall.(Nitrariaceae) is used to treat hypertension. This study determined the effects of the total alkaloids of the leaves of Nitraria sibirica(NSTA) on blood pressure...In traditional Chinese medicine, Nitraria sibirica Pall.(Nitrariaceae) is used to treat hypertension. This study determined the effects of the total alkaloids of the leaves of Nitraria sibirica(NSTA) on blood pressure and albuminuria in mice treated with angiotensin II and a high-salt diet(ANG/HS). Adult mice were divided into three groups: control; infused with angiotensin II and fed a diet containing 4% NaCl(ANG/HS; and ANG/HS plus injection of NSTA(1 mg·kg-1·d-1, i.p.). After treatment of these regimens, daily water and food intake, kidney weight, blood pressure, urinary albumin excretion, renal concentrations of inflammatory markers, including soluble intercellular adhesion molecule-1(sICAM-1) and monocyte chemoattractant protein-1(MCP-1), and the expression of renal fibrosis markers were determined. Compared to the control group, the ANG/HS group had higher blood pressure and urinary albumin excretion. Treatment with NSTA in ANG/HS mice for three weeks significantly reduced blood pressure and urinary albumin excretion. ANG/HS treatment caused elevated levels of sICAM-1 and MCP-1, as well as increased fibrosis markers. Concurrent treatment with ANG/HS and NSTA attenuated the levels and expression of renal inflammatory and fibrosis markers. Treatment with NSTA effectively reduces hypertension-induced albuminuria through the reduction of renal inflammatory and fibrosis markers.展开更多
This study addresses the adaptation of Nitraria sphaerocarpa to blown sand at the edge of a desert oasis with regard to the aspects of soil seed banks, seedlings, and population. Horizontally, the total number of seed...This study addresses the adaptation of Nitraria sphaerocarpa to blown sand at the edge of a desert oasis with regard to the aspects of soil seed banks, seedlings, and population. Horizontally, the total number of seeds per unit area decreased from the shrub canopy center to intershrub areas, and most seeds were found under shrub canopies. Vertically, the highest proportion of seeds was found at depths of 5-10 cm. The emergence percentage, seedling mass, and seedling height, which were significantly affected by both burial depth and seed size, were highest at the optimal burial depth of 2 cm, and decreased with increasing burial depth in each seed size-class. Although seedling mass was usually greatest for large seeds and least for small seeds at each burial depth, little difference was observed in seedling height at shallow burial depths of 0-3 cm. The population shows a patchy and discontinuous distribution pattern. Population height increases with increasing sand depth. Also the density increases with increasing depth of sand in the desert; however, there is a steady decrease when the depth of sand is more than 100 cm. This result indicates that the depth of sand that is most suitable for the growth of Nitraria sphaerocarpa is 100 cm. The size of the population is significantly correlated with the sand depth, which increases with increasing depth in the desert.展开更多
文摘OBJECTIVE To explore hypoglycemic effect of 95%ethanol fraction of Nitraria roborowskii Kom(NRK-C)and its possible mechanism evaluated in the type 2 diabetes mellitus(T2DM)mice.METHODS The body weight,organ indices,blood glucose levels,serum biochemical indexes,as well as HE/PAS histopathological section were all analyzed to assess the hypoglycemic effect of NRK-C in T2DM mice induced by a high-fat diet(HFD)combined with six intraperitoneal injections of 35 mg·kg^(-1)of streptozotocin(STZ).The Western blotting and immunofluorescence were further applied to determine the regulatory effect of NRK-C on key signaling proteins.RESULTS The fasting blood glucose levels were significantly reduced after 7 weeks of administration of NRK-C.In addition,NRK-C could also significantly improve glucose tolerance,hepatic glycogen levels,and lipid levels(total cholesterol,triglyceride,low density lipoprotein and high density lipoprotein),and significantly reduced insulin resistance of diabetic mice,which played an important role in the antidiabetic effects.Further mechanism research demonstrated that phosphorylated PI3K expression was up-regulated and p-GSK3βexpression was up-regulated after NRK-C intervention,indicating that NRK-C might exert a potential antidiabetic effect by modulating the PI3K/AKT signaling pathway.CONCLUSION All these results suggested that NRK-C might improve T2DM and had the potential to be used as an adjunctive therapy.
基金Supported by the Natural Science Foundation of Heilongjiang Province(LH2019C021)。
文摘Salt stress is a major abiotic stress limiting plant growth and yield. In the present study, the effects of exogenous H_(2)O_(2) on the reactive oxygen species(ROS) metabolism and the antioxidant system in leaves of Nitralia tangutorum Bobr. under salt stress were studied. N. tangutorum seedlings were subjected to 200 mmol·L^(-1) NaCl treatment with or without the exogenous application of H_(2)O_(2) for 7 days. The results showed that NaCl stress significantly increased the relative conductivity, the contents of thiobarbituric acid reactive substances(TBARS) and ROS(H_(2)O_(2) and O_(2)^(·-)), as well as promoted the activities of antioxidant enzymes including superoxide dismutase(SOD), peroxidase(POD), catalase(CAT), and ascorbate peroxidase(APX) in N. tangutorum leaves. In addition, exogenous H_(2)O_(2) decreased the relative conductivity, the contents of TBARS, H_(2)O_(2) and O_(2)^(·-), while further enhanced the activities of antioxidant enzymes. These results indicated that H_(2)O_(2) effectively alleviated the adverse effects of NaCl stress on N. tangutorum through the regulation of ROS metabolism.
基金Supported by the Special Scientific Research Project of Forestry Public Welfare Profession(200904033)The Project of Agricultural Science and Technology Achievements Transformation Fund(2011GB24320010)~~
文摘In this paper,six important distribution areas of Nitraria tangutorum Bobr.in Tsaidam Basin were selected as research objects,to study spatial variability and distribution of soil nutrients in different N.tangutorum populations and analyze the relationship between soil nutrient contents and geographical location,by measuring soil pH and the contents of organic matter（OM）,total nitrogen（N）,total phosphorus（P）,total potassium（K）,hydrolysis N,available P and available K in soils.Results showed that：（1） soil nutrient contents among different populations showed significant spatial variability,and soil depth had a significant effect on soil nutrients contents,but the variation rules were not obvious.（2）Average pH and average contents of OM,total N,total P,total K,hydrolysis N,available P and available K in soils with different depths（0-15,15-30,30-45 cm） varied in the range of 8.37-9.21,3.34-20.68,0.18-1.21,0.35-0.75,16.12-22.04,5.13-553.28,1.10-52.54 and 103.83-562.28 mg/kg,respectively.（3） The analysis results of correlation between average values of pH and contents of nutrient indexes in soils with different depths（0-15,15-30,30-45 cm） showed that the correlation of these indexes were different.（4）OM and total N contents in soils with different depths（0-15,15-30,30-45 cm） all had a significant positive correlation with latitude and negative correlation with longitude and altitude,and the correlation of available P and available K contents in surface soils（0-15 cm） with latitude,longitude and altitude were significant positive,significant negative and significant negative,respectively;moreover,longitude and latitude also showed a significant impact on soil available K contents with the depth of 30-45 cm.In addition,comprehensive analysis result of nutrient contents showed that N.tangutorum populations in Huaitou Tala Town had the highest fertility,and the fertility levels of N.tangutorum populations in Chaka Town and Wulan Keke Town were relatively lower.
基金supported by the National Basic Research Program of China (2009CB421303)the Strategic Priority Re- search Program–Climate Change: Carbon Budget and Relevant Issues of the Chinese Academy of Sciences (XDA05050406-1)the National Natural Science Foundation of China (40930636, 41240003, 41271061)
文摘Abstract： Soil water repellency （WR） is an important physical characteristic of soil surface. It is capable of largely influencing the hydrological and geomorphological processes of soil, as well as affecting the ecological processes of plants, such as growth and seed germination, and has thus been a hot topic in recent research around the world. In this paper, the capillary rise method was used to study the soil WR characteristics of Nitraria tangutorun nebkhas. Soil water repellencies at different succession stages of Nitraria tangutorun were investigated, and the relationships between soil WR and soil organic matter, total N, and total P, soil texture, pH, and concentrations of CO32, HCO3-, CI, SO42-, Na~, K~, Ca2~ and Mg2＋ were discussed. Soil WR may be demonstrated at the following nebkhas dune evolvement stages： extremely degraded〉degraded〉stabilized〉well developed〉newly developed〉quick sand. Apart from some soil at the bottom, the WR of other soils （crest and slope of dune） was found to be largest at the topsoil, and decreased as the soil depth increased. The results showed that multiple factors affected soil WR characteristics e.g. WR increased significantly as the contents of soil organic matter and total N increased, but did not change as the total P content increased. Soil texture was a key factor affecting soil WR; soil WR increased significantly as clay content increased, and decreased significantly as sand content increased. Low pH was shown to be more suitable for the occurrence of soil WR. Four cations （Ca2＋, Mg2＋, K＋ and Na＋） and two anions （CI and SO42） enhanced soil WR, while CO32-decreased it. HCO3- did not show any observable effect. Finally, we established a best-fit general linear model （GLM） between soil-air-water contact angle （CA） and influencing factors （CA=5.606 sand＋6.496 （clay and silt）-2.353 pH＋470.089 CQ2＋11.346 Na＋-407.707 Cl--14.245 SO42-＋0.734 total N-519.521 ）. It was concluded that all soils contain subcritical WR （0°〈CA〈90°）. The development and succession of Nitraria tangutorun nebkhas may improve the formation of soil subcritical WR. There exist significant relationships between soils subcritical WR and soil physical or chemical properties.
基金supported by the West Light Foundation of the Chinese Academy of Science(No.XBBS201011)AHA Grant-in-Aid(AHASE 0054)DODI grant from Georgia Health Sciences University to M.H.Wang
文摘In traditional Chinese medicine, Nitraria sibirica Pall.(Nitrariaceae) is used to treat hypertension. This study determined the effects of the total alkaloids of the leaves of Nitraria sibirica(NSTA) on blood pressure and albuminuria in mice treated with angiotensin II and a high-salt diet(ANG/HS). Adult mice were divided into three groups: control; infused with angiotensin II and fed a diet containing 4% NaCl(ANG/HS; and ANG/HS plus injection of NSTA(1 mg·kg-1·d-1, i.p.). After treatment of these regimens, daily water and food intake, kidney weight, blood pressure, urinary albumin excretion, renal concentrations of inflammatory markers, including soluble intercellular adhesion molecule-1(sICAM-1) and monocyte chemoattractant protein-1(MCP-1), and the expression of renal fibrosis markers were determined. Compared to the control group, the ANG/HS group had higher blood pressure and urinary albumin excretion. Treatment with NSTA in ANG/HS mice for three weeks significantly reduced blood pressure and urinary albumin excretion. ANG/HS treatment caused elevated levels of sICAM-1 and MCP-1, as well as increased fibrosis markers. Concurrent treatment with ANG/HS and NSTA attenuated the levels and expression of renal inflammatory and fibrosis markers. Treatment with NSTA effectively reduces hypertension-induced albuminuria through the reduction of renal inflammatory and fibrosis markers.
基金Project supported by the National Natural Science Foundation of China (No. 40571026).
文摘This study addresses the adaptation of Nitraria sphaerocarpa to blown sand at the edge of a desert oasis with regard to the aspects of soil seed banks, seedlings, and population. Horizontally, the total number of seeds per unit area decreased from the shrub canopy center to intershrub areas, and most seeds were found under shrub canopies. Vertically, the highest proportion of seeds was found at depths of 5-10 cm. The emergence percentage, seedling mass, and seedling height, which were significantly affected by both burial depth and seed size, were highest at the optimal burial depth of 2 cm, and decreased with increasing burial depth in each seed size-class. Although seedling mass was usually greatest for large seeds and least for small seeds at each burial depth, little difference was observed in seedling height at shallow burial depths of 0-3 cm. The population shows a patchy and discontinuous distribution pattern. Population height increases with increasing sand depth. Also the density increases with increasing depth of sand in the desert; however, there is a steady decrease when the depth of sand is more than 100 cm. This result indicates that the depth of sand that is most suitable for the growth of Nitraria sphaerocarpa is 100 cm. The size of the population is significantly correlated with the sand depth, which increases with increasing depth in the desert.
