Chemo-photothermal treatment is one of the most efficient strategies for cancer therapy.However,traditional drug carriers without near-infrared absorption capacity need to be loaded with materials behaving phototherma...Chemo-photothermal treatment is one of the most efficient strategies for cancer therapy.However,traditional drug carriers without near-infrared absorption capacity need to be loaded with materials behaving photothermal properties,as it results in complicated synthesis process,inefficient photothermal effects and hindered NIR-mediated drug release.Herein we report a facile synthesis of a polyethylene glycol(PEG)linked liposome(PEG-liposomes)coated doxorubicin(DOX)-loaded ordered mesoporous carbon(OMC)nanocomponents(PEG-LIP@OMC/DOX)by simply sonicating DOX and OMC in PEG-liposomes suspensions.The as-obtained PEG-LIP@OMC/DOX exhibits a nanoscale size(600±15 nm),a negative surface potential(-36.70 mV),high drug loading(131.590 mg/g OMC),and excellent photothermal properties.The PEG-LIP@OMC/DOX can deliver loaded DOX to human MCF-7 breast cancer cells(MCF-7)and the cell toxicity viability shows that DOX unloaded PEG-LIP@OMC has no cytotoxicity,confirming the PEG-LIP@OMC itself has excellent biocompatibility.The NIR-triggered release studies demonstrate that this NIR-responsive drug delivery system enables on-demand drug release.Furthermore,cell viability results using human MCF-7 cells demonstrated that the combination of NIR-based hyperthermal therapy and triggered chemothe rapy can provide higher therapeutic efficacy than re spective monothe rapies.With these excellent features,we believe that this phospholipid coating based multifunctional delivery system strategy should promote the application of OMC in nanomedical applications.展开更多
It is of great importance to treat a bacterial-infected wound by a smart dressing capable of delivering antibiotics in a smart manner without causing drug resistance.The construction of smart release nanocontainers re...It is of great importance to treat a bacterial-infected wound by a smart dressing capable of delivering antibiotics in a smart manner without causing drug resistance.The construction of smart release nanocontainers responsive to near-infrared(NIR)laser irradiation in an on-demand and stepwise way is a promising strategy for avoiding the emergence of multidrug-resistant bacteria.Here,we develop a hydrogel composite made of alginate and nanotubes with an efficient NIR-triggered release of rifampicin and outstanding antibacterial ability.This composite hydrogel is prepared through co-encapsulating antibacterial drug(rifampicin),NIR-absorbing dye(indocyanine green),and phase-change materials(a eutectic mixture of fatty acids)into halloysite nanotubes,followed by incorporation into alginate hydrogels,allowing the in-situ gelation at room temperature and maintaining the integrity of drug-loaded nanotubes.Among them,the eutectic mixture with a melting point of 39℃ serves as the biocompatible phase-change material to facilitate the NIR-triggered drug release.The resultant phase-change material gated-nanotubes exhibit a prominent photothermal efficiency with multistep drug release under laser irradiation.In an in vitro assay,composite hydrogel provides good antibacterial potency against Staphylococcus aureus,one of the most prevalent microorganisms of dangerous gas gangrene.A bacterial-infected rat full-thickness wound model demonstrates that the NIR-responsive composite hydrogel inhibits the bacteria colonization and suppresses the inflammatory response caused by bacteria,promoting angiogenesis and collagen deposition to accelerate wound regeneration.The NIR-responsive composite hydrogel has a great po-tential as an antibacterial wound dressing functionalized with controlled multistep treatment of the infected sites.展开更多
Gaseous therapy based on nitric oxide(NO),as a potential anti-tumor treatment strategy,has attracted great attention,but the targeted and controlled gas release in the tumor site still remains a challenge.In addressin...Gaseous therapy based on nitric oxide(NO),as a potential anti-tumor treatment strategy,has attracted great attention,but the targeted and controlled gas release in the tumor site still remains a challenge.In addressing these difficulties,a near-infrared(NIR)light-triggered NO release nanogenerator with a“linkage mechanism”was designed on the basis of sodium nitroprussidedoped mesoporous Prussian blue nanoparticles,in which the outer structure was modified with p H-sensitive gatekeeper chitosan and tumor-targeting agent folic acid.The“linkage mechanism”can achieve precise release of NO under the control of photothermal effect at tumor site,which can couple photothermal therapy and gas therapy to address the premature release of gas during transportation.Meanwhile,the amount of released gas can be controlled by adjusting the irradiation time and laser intensity.Furthermore,as-fabricated nanocomposites hold high photothermal conversion efficiency under NIR laser irradiation,resulting in the on-demand release of NO and chemotherapy drugs.The released NO can inhibit the expression of hypoxiainducible factorα(HIF-1α)and alleviate the hypoxic tumor microenvironment,thereby enhancing the efficacy of chemotherapy.Moreover,in vitro and in vivo experiments exhibited remarkable antitumor efficiency,and the synergistic gas/chemo/photothermal therapy of deep tumors was achieved.These findings indicate an effective strategy to stimulate further the development of deep tumor therapy,which may provide new insights into other NO-related medical applications.展开更多
基金the National Natural Science Foundation of China(Nos.21735002,21521063,21675046,21874035,21806186 and 21775036)the Natural Science Foundation of Hunan Province,China(No.2018JJ2033)the Key Point Research and Invention Program of Hunan Province,China(No.2017DK2011)。
文摘Chemo-photothermal treatment is one of the most efficient strategies for cancer therapy.However,traditional drug carriers without near-infrared absorption capacity need to be loaded with materials behaving photothermal properties,as it results in complicated synthesis process,inefficient photothermal effects and hindered NIR-mediated drug release.Herein we report a facile synthesis of a polyethylene glycol(PEG)linked liposome(PEG-liposomes)coated doxorubicin(DOX)-loaded ordered mesoporous carbon(OMC)nanocomponents(PEG-LIP@OMC/DOX)by simply sonicating DOX and OMC in PEG-liposomes suspensions.The as-obtained PEG-LIP@OMC/DOX exhibits a nanoscale size(600±15 nm),a negative surface potential(-36.70 mV),high drug loading(131.590 mg/g OMC),and excellent photothermal properties.The PEG-LIP@OMC/DOX can deliver loaded DOX to human MCF-7 breast cancer cells(MCF-7)and the cell toxicity viability shows that DOX unloaded PEG-LIP@OMC has no cytotoxicity,confirming the PEG-LIP@OMC itself has excellent biocompatibility.The NIR-triggered release studies demonstrate that this NIR-responsive drug delivery system enables on-demand drug release.Furthermore,cell viability results using human MCF-7 cells demonstrated that the combination of NIR-based hyperthermal therapy and triggered chemothe rapy can provide higher therapeutic efficacy than re spective monothe rapies.With these excellent features,we believe that this phospholipid coating based multifunctional delivery system strategy should promote the application of OMC in nanomedical applications.
基金National Natural Science Foundation of China(Grant No.82002049 and 52073014,to J.X.)Key Program of Beijing Natural Science Foundation(Z200025)+1 种基金Fundamental Research Funds for the Central Universities(buctrc202020)YL thanks support by T.Pipes Nano Microsystem Endowment,Louisiana Tech University,USA.
文摘It is of great importance to treat a bacterial-infected wound by a smart dressing capable of delivering antibiotics in a smart manner without causing drug resistance.The construction of smart release nanocontainers responsive to near-infrared(NIR)laser irradiation in an on-demand and stepwise way is a promising strategy for avoiding the emergence of multidrug-resistant bacteria.Here,we develop a hydrogel composite made of alginate and nanotubes with an efficient NIR-triggered release of rifampicin and outstanding antibacterial ability.This composite hydrogel is prepared through co-encapsulating antibacterial drug(rifampicin),NIR-absorbing dye(indocyanine green),and phase-change materials(a eutectic mixture of fatty acids)into halloysite nanotubes,followed by incorporation into alginate hydrogels,allowing the in-situ gelation at room temperature and maintaining the integrity of drug-loaded nanotubes.Among them,the eutectic mixture with a melting point of 39℃ serves as the biocompatible phase-change material to facilitate the NIR-triggered drug release.The resultant phase-change material gated-nanotubes exhibit a prominent photothermal efficiency with multistep drug release under laser irradiation.In an in vitro assay,composite hydrogel provides good antibacterial potency against Staphylococcus aureus,one of the most prevalent microorganisms of dangerous gas gangrene.A bacterial-infected rat full-thickness wound model demonstrates that the NIR-responsive composite hydrogel inhibits the bacteria colonization and suppresses the inflammatory response caused by bacteria,promoting angiogenesis and collagen deposition to accelerate wound regeneration.The NIR-responsive composite hydrogel has a great po-tential as an antibacterial wound dressing functionalized with controlled multistep treatment of the infected sites.
基金supported by the National Natural Science Foundation of China(21908059)the China Postdoctoral Science Foundation(2019M651419)+5 种基金the Shanghai Sailing Program(19YF1410900,21YF1451700)the Natural Science Foundation of Shanghai(22ZR1415400)the Fundamental Research Funds for the Central Universities(22221818014)the Shanghai Post-doctoral Excellence Program(2018011)the Foundation of State Key Laboratory of Biobased Material and Green Papermaking,Qilu University of Technology,Shandong Academy of Sciences(GZKF202031)the Open Funding Project of the State Key Laboratory of Bioreactor Engineering。
文摘Gaseous therapy based on nitric oxide(NO),as a potential anti-tumor treatment strategy,has attracted great attention,but the targeted and controlled gas release in the tumor site still remains a challenge.In addressing these difficulties,a near-infrared(NIR)light-triggered NO release nanogenerator with a“linkage mechanism”was designed on the basis of sodium nitroprussidedoped mesoporous Prussian blue nanoparticles,in which the outer structure was modified with p H-sensitive gatekeeper chitosan and tumor-targeting agent folic acid.The“linkage mechanism”can achieve precise release of NO under the control of photothermal effect at tumor site,which can couple photothermal therapy and gas therapy to address the premature release of gas during transportation.Meanwhile,the amount of released gas can be controlled by adjusting the irradiation time and laser intensity.Furthermore,as-fabricated nanocomposites hold high photothermal conversion efficiency under NIR laser irradiation,resulting in the on-demand release of NO and chemotherapy drugs.The released NO can inhibit the expression of hypoxiainducible factorα(HIF-1α)and alleviate the hypoxic tumor microenvironment,thereby enhancing the efficacy of chemotherapy.Moreover,in vitro and in vivo experiments exhibited remarkable antitumor efficiency,and the synergistic gas/chemo/photothermal therapy of deep tumors was achieved.These findings indicate an effective strategy to stimulate further the development of deep tumor therapy,which may provide new insights into other NO-related medical applications.
