This work shows that novel virus-like mesopore silica-zinc oxide/Ag nanoparticles (SZnOAg) synthesized and professionally collected on NIR laser irradiation with quercetin to improve the elimination the mutated virus ...This work shows that novel virus-like mesopore silica-zinc oxide/Ag nanoparticles (SZnOAg) synthesized and professionally collected on NIR laser irradiation with quercetin to improve the elimination the mutated virus as a biomedical application. A unique type of silica nanoparticles with a self-in- flating tubular surface has been successfully synthesized using a novel single-micelle epitaxial growth process. The properties of the nanoparticles can be tuned with respect to their core diameter, tubular length, and outer diameter. Due to their biomimetic appearance, they can rapidly transform living cells into virus-like particles, this SZnOAg nanomaterial has specific elimination effect on bacteriophage and Covid-19. Using epitaxial growth, we can construct virus-like structures that can be used for biomedicine applications. These nanomaterials and NIR laser could open the way to a new range of antiviral materials, due to the low-efficiency cellular uptake of current nanoparticles, their applications in the biomedical field are limited. Herein, it clearly shows that novel mesoporous silica nanoparticles can be easily exhibited superior cellular uptake property.展开更多
Photothermal therapy(PTT)using near-infrared(NIR)light for tumor treatment has triggered extensive attentions because of its advantages of noninvasion and convenience.The current research on PTT usually uses lasers in...Photothermal therapy(PTT)using near-infrared(NIR)light for tumor treatment has triggered extensive attentions because of its advantages of noninvasion and convenience.The current research on PTT usually uses lasers in the first NIR window(NIR-I;700–900 nm)as irradiation source.However,the second NIR window(NIR-II;1000–1700 nm)especially NIRIIa window(1300–1400 nm)is considered much more promising in diagnosis and treatment as its superiority in penetration depth and maximum permissible exposure over NIR-I window.Hereby,we propose the use of laser excitation at 1275 nm,which is approved by Food and Drug Administration for physical therapy,as an attractive technique for PTT to balance of tissue absorption and scattering with water absorption.Specifically,CuS-PEG nanoparticles with similar absorption values at 1275 and 808 nm,a conventional NIR-I window for PTT,were synthesized as PTT agents and a comparison platform,to explore the potential of 1275 and 808 nm lasers for PTT,especially in deep-tissue settings.The results showed that 1275 nm laser was practicable in PTT.It exhibited much more desirable outcomes in cell ablation in vitro and deep-tissue antitumor capabilities in vivo compared to that of 808 nm laser.NIR-IIa laser illumination is superior to NIR-I laser for deep-tissue PTT,and shows high potential to improve the PTT outcome.展开更多
In this work, a 532 nm diode CW laser is used to heat samples used as building materials at a 1 meter standoff distance while using an FLIR (Forward-Looking Infrared) thermal camera to monitor and record the heating a...In this work, a 532 nm diode CW laser is used to heat samples used as building materials at a 1 meter standoff distance while using an FLIR (Forward-Looking Infrared) thermal camera to monitor and record the heating and then cooling of each sample after lasers are switched off. The data is then analyzed using FLIR proprietary software. Since the absorption spectra of materials are unique, using multiple lasers of different wavelengths to simultaneously shine onto the sample at different locations would give enough thermal data to successfully characterize the samples within a reasonable amount of time. The results are very promising for applications involving non-destructive detection and classification of materials.展开更多
Direct analysis of copper-base alloys using laser ablation techniques is an increasingly common procedure in cultural heritage studies. However, main discussions remain focused on the possibility of using non-matrix m...Direct analysis of copper-base alloys using laser ablation techniques is an increasingly common procedure in cultural heritage studies. However, main discussions remain focused on the possibility of using non-matrix matched external reference materials. To evaluate the occurrence of matrix effects during in situ microanalysis of copper-base materials, using near infrared femtosecond laser ablation techniques (NIR fs-LA-ICP-MS), two bronzes, i.e., (Sn-Zn)-ternary and (Sn)-binary copper-matrix reference materials, as well as a reference synthetic glass (NIST-SRM-610) have been analyzed. The results have been compared to data obtained on a sulfide-matrix reference material. Similar values in relative sensitivity averages of 63Cu, 118Sn and 66Zn, as well as in 118Sn/63Cu and 66Zn/63Cu ratios were obtained, for all analyzed matrix types, i.e., copper-base-, silicate-, and sulfide-reference materials. Consequently, it is possible to determinate major and minor element concentrations in copper alloys, i.e., Cu, Sn and Zn, using silicate and sulfide reference materials as external calibrators, without any matrix effect and over a wide range of concentrations (from wt.% to ppm). Equally, Cu, Sn and Zn concentrations can be precisely determined in sulfides using homogeneous alloys (reference) materials as an external calibrator. Thus, it is possible to determine Cu, Sn and Zn in copper-base materials and their ore minerals, mostly sulfides, in a single analytical session, without requiring specific external calibrators for each matrix type. In contrast, immiscible elements in copper matrix, such as Pb and Fe show notable differences in their relative sensitivity values and ratios for different matrix-materials analyzed, implying that matrix-matched external calibrations remain to be applied for their trace quantification.展开更多
Photothermal therapy is emerging as a very promising way for minimally invasive cancer treatment.To enhance thermal energy deposition of laser in target malignant tissues,liquid metal nanoparticles(LMNPs)have been rec...Photothermal therapy is emerging as a very promising way for minimally invasive cancer treatment.To enhance thermal energy deposition of laser in target malignant tissues,liquid metal nanoparticles(LMNPs)have been recently identified as completely unprecedented photothermal sensitizers due to their unique physicochemical properties and superior photothermal conversion rate under near-infrared(NIR)laser irradiation.However,there is currently a strong lack of understanding of the laser energy distribution and the transient temperature field within the biological tissues,which would seriously hinder the development of LMNPs assisted photothermal therapy.Therefore,this paper focused on the distinctive photothermal effect of LMNPs embedded in biological tissues under NIR laser irradiation.The mathematical model coupling the Monte-Carlo photon transport model with Penne's bioheat transfer model has been established.Simulation studies have shown that LMNPs play an important role in enhancing the absorption of NIR laser,which contributes to local temperature rise and improves the temperature distribution.Comparing with the control case without LMNPs,the maximum temperature increases by nearly 1.0 time,the local temperature rise reaches 30℃ in 1.0 second.When the diameter and concentration of LMNPs are 40 nm and 1012/mm3,the resulting temperature variation and distribution is best for the effective killing of tumors without damaging normal tissues.In addition,the simulation results are meaningful for guiding the selection of laser irradiation time in conjunction with the cooling time,ensuring the controllable accuracy of treatment.To the best of our knowledge,the present study is one of the first attempts to quantify the influence of transformable LMNPs on the temperature distributions inside the biological tissues,showing important academic significance for guiding LMNPs assisted photothermal treatment.展开更多
Infected wounds pose a significant clinical challenge due to bacterial resistance, recurrent infections, and impaired healing. Reactive oxygen species (ROS)-based strategies have shown promise in eradicating bacterial...Infected wounds pose a significant clinical challenge due to bacterial resistance, recurrent infections, and impaired healing. Reactive oxygen species (ROS)-based strategies have shown promise in eradicating bacterial infections. However, the excess ROS in the infection site after treatments may cause irreversible damage to healthy tissues. To address this issue, we developed bovine serum albumin-iridium oxide nanoclusters (BSA-IrOx NCs) which enable photo-regulated ROS generation and scavenging using near infrared (NIR) laser. Upon NIR laser irradiation, BSA-IrOx NCs exhibit enhanced photodynamic therapy, destroying biofilms and killing bacteria. When the NIR laser is off, the nanoclusters' antioxidant enzyme-like activities prevent inflammation and repair damaged tissue through ROS clearance. Transcriptomic and metabolomic analyses revealed that BSA-IrOx NCs inhibit bacterial nitric oxide synthase, blocking bacterial growth and biofilm formation. Furthermore, the nanoclusters repair impaired skin by strengthening cell junctions and reducing mitochondrial damage in a fibroblast model. In vivo studies using rat infected wound models confirmed the efficacy of BSA-IrOx NCs. This study presents a promising strategy for treating biofilm-induced infected wounds by regulating the ROS microenvironment, addressing the challenges associated with current ROS-based antibacterial approaches.展开更多
文摘This work shows that novel virus-like mesopore silica-zinc oxide/Ag nanoparticles (SZnOAg) synthesized and professionally collected on NIR laser irradiation with quercetin to improve the elimination the mutated virus as a biomedical application. A unique type of silica nanoparticles with a self-in- flating tubular surface has been successfully synthesized using a novel single-micelle epitaxial growth process. The properties of the nanoparticles can be tuned with respect to their core diameter, tubular length, and outer diameter. Due to their biomimetic appearance, they can rapidly transform living cells into virus-like particles, this SZnOAg nanomaterial has specific elimination effect on bacteriophage and Covid-19. Using epitaxial growth, we can construct virus-like structures that can be used for biomedicine applications. These nanomaterials and NIR laser could open the way to a new range of antiviral materials, due to the low-efficiency cellular uptake of current nanoparticles, their applications in the biomedical field are limited. Herein, it clearly shows that novel mesoporous silica nanoparticles can be easily exhibited superior cellular uptake property.
基金supported,in part,by the Natural Science Foundation of China (Nos.81402043 and 81201141)the Clinical Capability Construction Project for Liaoning Provincial Hospitals (LNCCC-D50-2015+1 种基金LNCCC-C09-2015)the China postdoctoral science foundation Grant (2016T90233)
文摘Photothermal therapy(PTT)using near-infrared(NIR)light for tumor treatment has triggered extensive attentions because of its advantages of noninvasion and convenience.The current research on PTT usually uses lasers in the first NIR window(NIR-I;700–900 nm)as irradiation source.However,the second NIR window(NIR-II;1000–1700 nm)especially NIRIIa window(1300–1400 nm)is considered much more promising in diagnosis and treatment as its superiority in penetration depth and maximum permissible exposure over NIR-I window.Hereby,we propose the use of laser excitation at 1275 nm,which is approved by Food and Drug Administration for physical therapy,as an attractive technique for PTT to balance of tissue absorption and scattering with water absorption.Specifically,CuS-PEG nanoparticles with similar absorption values at 1275 and 808 nm,a conventional NIR-I window for PTT,were synthesized as PTT agents and a comparison platform,to explore the potential of 1275 and 808 nm lasers for PTT,especially in deep-tissue settings.The results showed that 1275 nm laser was practicable in PTT.It exhibited much more desirable outcomes in cell ablation in vitro and deep-tissue antitumor capabilities in vivo compared to that of 808 nm laser.NIR-IIa laser illumination is superior to NIR-I laser for deep-tissue PTT,and shows high potential to improve the PTT outcome.
文摘In this work, a 532 nm diode CW laser is used to heat samples used as building materials at a 1 meter standoff distance while using an FLIR (Forward-Looking Infrared) thermal camera to monitor and record the heating and then cooling of each sample after lasers are switched off. The data is then analyzed using FLIR proprietary software. Since the absorption spectra of materials are unique, using multiple lasers of different wavelengths to simultaneously shine onto the sample at different locations would give enough thermal data to successfully characterize the samples within a reasonable amount of time. The results are very promising for applications involving non-destructive detection and classification of materials.
文摘Direct analysis of copper-base alloys using laser ablation techniques is an increasingly common procedure in cultural heritage studies. However, main discussions remain focused on the possibility of using non-matrix matched external reference materials. To evaluate the occurrence of matrix effects during in situ microanalysis of copper-base materials, using near infrared femtosecond laser ablation techniques (NIR fs-LA-ICP-MS), two bronzes, i.e., (Sn-Zn)-ternary and (Sn)-binary copper-matrix reference materials, as well as a reference synthetic glass (NIST-SRM-610) have been analyzed. The results have been compared to data obtained on a sulfide-matrix reference material. Similar values in relative sensitivity averages of 63Cu, 118Sn and 66Zn, as well as in 118Sn/63Cu and 66Zn/63Cu ratios were obtained, for all analyzed matrix types, i.e., copper-base-, silicate-, and sulfide-reference materials. Consequently, it is possible to determinate major and minor element concentrations in copper alloys, i.e., Cu, Sn and Zn, using silicate and sulfide reference materials as external calibrators, without any matrix effect and over a wide range of concentrations (from wt.% to ppm). Equally, Cu, Sn and Zn concentrations can be precisely determined in sulfides using homogeneous alloys (reference) materials as an external calibrator. Thus, it is possible to determine Cu, Sn and Zn in copper-base materials and their ore minerals, mostly sulfides, in a single analytical session, without requiring specific external calibrators for each matrix type. In contrast, immiscible elements in copper matrix, such as Pb and Fe show notable differences in their relative sensitivity values and ratios for different matrix-materials analyzed, implying that matrix-matched external calibrations remain to be applied for their trace quantification.
基金the National Key R&D Program of China(No.2018YFC1705106)National Natural Science Foundation of China(No.51890893)。
文摘Photothermal therapy is emerging as a very promising way for minimally invasive cancer treatment.To enhance thermal energy deposition of laser in target malignant tissues,liquid metal nanoparticles(LMNPs)have been recently identified as completely unprecedented photothermal sensitizers due to their unique physicochemical properties and superior photothermal conversion rate under near-infrared(NIR)laser irradiation.However,there is currently a strong lack of understanding of the laser energy distribution and the transient temperature field within the biological tissues,which would seriously hinder the development of LMNPs assisted photothermal therapy.Therefore,this paper focused on the distinctive photothermal effect of LMNPs embedded in biological tissues under NIR laser irradiation.The mathematical model coupling the Monte-Carlo photon transport model with Penne's bioheat transfer model has been established.Simulation studies have shown that LMNPs play an important role in enhancing the absorption of NIR laser,which contributes to local temperature rise and improves the temperature distribution.Comparing with the control case without LMNPs,the maximum temperature increases by nearly 1.0 time,the local temperature rise reaches 30℃ in 1.0 second.When the diameter and concentration of LMNPs are 40 nm and 1012/mm3,the resulting temperature variation and distribution is best for the effective killing of tumors without damaging normal tissues.In addition,the simulation results are meaningful for guiding the selection of laser irradiation time in conjunction with the cooling time,ensuring the controllable accuracy of treatment.To the best of our knowledge,the present study is one of the first attempts to quantify the influence of transformable LMNPs on the temperature distributions inside the biological tissues,showing important academic significance for guiding LMNPs assisted photothermal treatment.
基金Institutional Research Project of Shanghai Sixth People's Hospital(LY33.X-4020).
文摘Infected wounds pose a significant clinical challenge due to bacterial resistance, recurrent infections, and impaired healing. Reactive oxygen species (ROS)-based strategies have shown promise in eradicating bacterial infections. However, the excess ROS in the infection site after treatments may cause irreversible damage to healthy tissues. To address this issue, we developed bovine serum albumin-iridium oxide nanoclusters (BSA-IrOx NCs) which enable photo-regulated ROS generation and scavenging using near infrared (NIR) laser. Upon NIR laser irradiation, BSA-IrOx NCs exhibit enhanced photodynamic therapy, destroying biofilms and killing bacteria. When the NIR laser is off, the nanoclusters' antioxidant enzyme-like activities prevent inflammation and repair damaged tissue through ROS clearance. Transcriptomic and metabolomic analyses revealed that BSA-IrOx NCs inhibit bacterial nitric oxide synthase, blocking bacterial growth and biofilm formation. Furthermore, the nanoclusters repair impaired skin by strengthening cell junctions and reducing mitochondrial damage in a fibroblast model. In vivo studies using rat infected wound models confirmed the efficacy of BSA-IrOx NCs. This study presents a promising strategy for treating biofilm-induced infected wounds by regulating the ROS microenvironment, addressing the challenges associated with current ROS-based antibacterial approaches.
