The principle of genomic selection(GS) entails estimating breeding values(BVs) by summing all the SNP polygenic effects. The visible/near-infrared spectroscopy(VIS/NIRS) wavelength and abundance values can directly re...The principle of genomic selection(GS) entails estimating breeding values(BVs) by summing all the SNP polygenic effects. The visible/near-infrared spectroscopy(VIS/NIRS) wavelength and abundance values can directly reflect the concentrations of chemical substances, and the measurement of meat traits by VIS/NIRS is similar to the processing of genomic selection data by summing all ‘polygenic effects' associated with spectral feature peaks. Therefore, it is meaningful to investigate the incorporation of VIS/NIRS information into GS models to establish an efficient and low-cost breeding model. In this study, we measured 6 meat quality traits in 359Duroc×Landrace×Yorkshire pigs from Guangxi Zhuang Autonomous Region, China, and genotyped them with high-density SNP chips. According to the completeness of the information for the target population, we proposed 4breeding strategies applied to different scenarios: Ⅰ, only spectral and genotypic data exist for the target population;Ⅱ, only spectral data exist for the target population;Ⅲ, only spectral and genotypic data but with different prediction processes exist for the target population;and Ⅳ, only spectral and phenotypic data exist for the target population.The 4 scenarios were used to evaluate the genomic estimated breeding value(GEBV) accuracy by increasing the VIS/NIR spectral information. In the results of the 5-fold cross-validation, the genetic algorithm showed remarkable potential for preselection of feature wavelengths. The breeding efficiency of Strategies Ⅱ, Ⅲ, and Ⅳ was superior to that of traditional GS for most traits, and the GEBV prediction accuracy was improved by 32.2, 40.8 and 15.5%, respectively on average. Among them, the prediction accuracy of Strategy Ⅱ for fat(%) even improved by 50.7% compared to traditional GS. The GEBV prediction accuracy of Strategy Ⅰ was nearly identical to that of traditional GS, and the fluctuation range was less than 7%. Moreover, the breeding cost of the 4 strategies was lower than that of traditional GS methods, with Strategy Ⅳ being the lowest as it did not require genotyping.Our findings demonstrate that GS methods based on VIS/NIRS data have significant predictive potential and are worthy of further research to provide a valuable reference for the development of effective and affordable breeding strategies.展开更多
Plants play a crucial role in maintaining ecological balance and biodiversity.However,plant health is easily affected by environmental stresses.Hence,the rapid and precise monitoring of plant health is crucial for glo...Plants play a crucial role in maintaining ecological balance and biodiversity.However,plant health is easily affected by environmental stresses.Hence,the rapid and precise monitoring of plant health is crucial for global food security and ecological balance.Currently,traditional detection strategies for monitoring plant health mainly rely on expensive equipment and complex operational procedures,which limit their widespread application.Fortunately,near-infrared(NIR)fluorescence and surface-enhanced Raman scattering(SERS)techniques have been recently highlighted in plants.NIR fluorescence imaging holds the advantages of being non-invasive,high-resolution and real-time,which is suitable for rapid screening in large-scale scenarios.While SERS enables highly sensitive and specific detection of trace chemical substances within plant tissues.Therefore,the complementarity of NIR fluorescence and SERS modalities can provide more comprehensive and accurate information for plant disease diagnosis and growth status monitoring.This article summarizes these two modalities in plant applications,and discusses the advantages of multimodal NIR fluorescence/SERS for a better understanding of a plant’s response to stress,thereby improving the accuracy and sensitivity of detection.展开更多
Acute lung injury(ALI)was characterized by excessive reactive oxygen species(ROS)levels and inflammatory response in the lung.Scavenging ROS could inhibit the excessive inflammatory response,further treating ALI.Herei...Acute lung injury(ALI)was characterized by excessive reactive oxygen species(ROS)levels and inflammatory response in the lung.Scavenging ROS could inhibit the excessive inflammatory response,further treating ALI.Herein,we designed a novel nanozyme(P@Co)comprised of polydopamine(PDA)nanoparticles(NPs)loading with ultra-small Co,combining with near infrared(NIR)irradiation,which could efficiently scavenge intracellular ROS and suppress inflammatory responses against ALI.For lipopolysaccharide(LPS)induced macrophages,P@Co+NIR presented excellent antioxidant and anti-inflammatory capacities through lowering intracellular ROS levels,decreasing the expression levels of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)as well as inducing macrophage M2 directional polarization.Significantly,it displayed the outstanding activities of lowering acute lung inflammation,relieving diffuse alveolar damage,and up-regulating heat shock protein 70(HSP70)expression,resulting in synergistic enhanced ALI therapy effect.It offers a novel strategy for the clinical treatment of ROS related diseases.展开更多
Near-infrared(NIR)theranostics have received considerable attention because of their advantages in precise diagnostic imaging and efficient simultaneous treatment and have achieved tremendous advancements in the last ...Near-infrared(NIR)theranostics have received considerable attention because of their advantages in precise diagnostic imaging and efficient simultaneous treatment and have achieved tremendous advancements in the last few years.However,their progress is severely restricted by the rarity of efficient second NIR(NIR-Ⅱ)responsive phototheranostic materials,especially in the NIR-Ⅱb region.Moreover,these materials often embarrass the quenching puzzle in the aggregative state,thus greatly reducing their theranostic performance.To overcome this limitation,we developed anti-quenching donor-acceptor-donor(D-AD)-conjugated oligomers with NIR-Ⅱb emission for high-performance NIR-Ⅱangiography and phototheranostics.Through multi-acceptor engineering,a series of multi-acceptor conjugated oligomer SU-n(n=1,2,and 5)with tunable acceptor ratios were synthesized,and their efficiency in anti-quenching NIR-Ⅱemission was demonstrated.When prepared into water-dispersed nanoparticles(NPs),SU-5 NPs exhibit bright NIR-Ⅱemission and dual phototherapy for photothermal therapy and photodynamic therapy simultaneously upon 808 nm light excitation.With these benefits,high-resolution whole-body and local angiography in vivo of SU-5 NPs were successfully realized in the NIR-Ⅱb window.Moreover,in vivo,theranostics experiments demonstrated the efficiency of SU-5 NPs in NIR-Ⅱimaging-guided complete tumor photoablation without any relapses with high biosafety.This work explores a practical multi-acceptor engineering strategy for developing anti-quenching theranostic materials,providing an efficient theranostic agent for efficient NIR-Ⅱb bioimaging and phototheranostics.展开更多
Rare earth luminescent materials have attracted extensive attention in the biomedical field as noncontact temperature monitoring devices with microscopic resolution due to their properties in the visible and near-infr...Rare earth luminescent materials have attracted extensive attention in the biomedical field as noncontact temperature monitoring devices with microscopic resolution due to their properties in the visible and near-infrared regions.At the application level,it is required to have a certain temperature monitoring capability in the near-infrared region II window to enhance the tissue penetration depth.Here,two kinds of YOFs:Er^(3+),Yb^(3+)were prepared by co-precipitation and hydrothermal method,and the luminescence was enhanced by ion doping.An Er^(3+)-based ratiometric nanothermometer of ^(4)F_(9∕2)→4 I_(15∕2)(672nm,upconversion luminescence)to ^(4)I _(13∕2)→^(4) I_(15∕2)(1580nm/1566nm,NIR II downshifting luminescence)were designed with the Stark energy level.When doped with 2%Zn^(2+),the relative temperature sensitivity of YOF prepared by co-precipitation method was improved from 0.30%℃^(-1)(30℃)to 0.59%℃^(-1)(30℃),expanding its use as a temperature monitoring device possibility.The temperature sensitivity of YOF prepared by hydrothermal method was 1.01%℃^(-1)(30℃).Finally,the NIR II luminescence of the prepared nanothermometer was used as a control for temperature monitoring of heating sites in mice.The results showed that it can distinguish heating site from control site and no significant cytotoxicity or damage to the tissues was revealed,indicating its broad prospects in the biomedical field and other temperature monitoring scenarios in the future.展开更多
基金the National Key R&D Program of China(2022YFB3206000)the National Natural Science Foundation of China(U23A20487)+1 种基金Dr.Li Dak Sum&Yip Yio Chin Development Fund for Regenerative Medicine,Zhejiang Universitythe National Natural Science Foundation of China(61975172).
文摘近红外二区(the second near-infrared window,NIR-II,900~1880 nm)荧光成像具有信号背景比高、穿透深度大的优势,在生物医学领域具有广泛的应用前景。对NIR-II成像窗口的划分有利于优化成像过程,其中,NIRIIx(1400~1500 nm)成像窗口得益于其独特的水吸收,可以有效抑制散射背景,实现高对比度成像。文章通过仿真模拟和活体实验,系统评估了NIR-IIx窗口的成像潜力。为了更好地推进NIR-IIx荧光成像的临床转化,研究采用美国食品及药物管理局(FDA)批准的有机小分子染料吲哚菁绿(Indocyanine Green,ICG)作为荧光探针,利用其延伸至NIR-II的荧光发射拖尾,实现了高对比度和清晰度的小鼠血管和肠道的NIR-IIx成像。此外,研究还结合同样通过FDA认证的亚甲基蓝(Methylene Blue,MB),成功实现了高质量的NIR-II双通道成像,精准定位小鼠的血管和淋巴结。文章进一步挖掘了NIR-IIx在生物成像上的独特优势和临床应用的潜力,并为NIR-II荧光成像的临床转化提供了重要参考。
基金supported by the National Natural Science Foundation of China(32160782 and 32060737).
文摘The principle of genomic selection(GS) entails estimating breeding values(BVs) by summing all the SNP polygenic effects. The visible/near-infrared spectroscopy(VIS/NIRS) wavelength and abundance values can directly reflect the concentrations of chemical substances, and the measurement of meat traits by VIS/NIRS is similar to the processing of genomic selection data by summing all ‘polygenic effects' associated with spectral feature peaks. Therefore, it is meaningful to investigate the incorporation of VIS/NIRS information into GS models to establish an efficient and low-cost breeding model. In this study, we measured 6 meat quality traits in 359Duroc×Landrace×Yorkshire pigs from Guangxi Zhuang Autonomous Region, China, and genotyped them with high-density SNP chips. According to the completeness of the information for the target population, we proposed 4breeding strategies applied to different scenarios: Ⅰ, only spectral and genotypic data exist for the target population;Ⅱ, only spectral data exist for the target population;Ⅲ, only spectral and genotypic data but with different prediction processes exist for the target population;and Ⅳ, only spectral and phenotypic data exist for the target population.The 4 scenarios were used to evaluate the genomic estimated breeding value(GEBV) accuracy by increasing the VIS/NIR spectral information. In the results of the 5-fold cross-validation, the genetic algorithm showed remarkable potential for preselection of feature wavelengths. The breeding efficiency of Strategies Ⅱ, Ⅲ, and Ⅳ was superior to that of traditional GS for most traits, and the GEBV prediction accuracy was improved by 32.2, 40.8 and 15.5%, respectively on average. Among them, the prediction accuracy of Strategy Ⅱ for fat(%) even improved by 50.7% compared to traditional GS. The GEBV prediction accuracy of Strategy Ⅰ was nearly identical to that of traditional GS, and the fluctuation range was less than 7%. Moreover, the breeding cost of the 4 strategies was lower than that of traditional GS methods, with Strategy Ⅳ being the lowest as it did not require genotyping.Our findings demonstrate that GS methods based on VIS/NIRS data have significant predictive potential and are worthy of further research to provide a valuable reference for the development of effective and affordable breeding strategies.
基金funded by the National Natural Science Foundation of China(Nos.22374055,22022404,22074050,82172055)the National Natural Science Foundation of Hubei Province(No.22022CFA033)the Fundamental Research Funds for the Central Universities(Nos.CCNU24JCPT001,CCNU24JCPT020)。
文摘Plants play a crucial role in maintaining ecological balance and biodiversity.However,plant health is easily affected by environmental stresses.Hence,the rapid and precise monitoring of plant health is crucial for global food security and ecological balance.Currently,traditional detection strategies for monitoring plant health mainly rely on expensive equipment and complex operational procedures,which limit their widespread application.Fortunately,near-infrared(NIR)fluorescence and surface-enhanced Raman scattering(SERS)techniques have been recently highlighted in plants.NIR fluorescence imaging holds the advantages of being non-invasive,high-resolution and real-time,which is suitable for rapid screening in large-scale scenarios.While SERS enables highly sensitive and specific detection of trace chemical substances within plant tissues.Therefore,the complementarity of NIR fluorescence and SERS modalities can provide more comprehensive and accurate information for plant disease diagnosis and growth status monitoring.This article summarizes these two modalities in plant applications,and discusses the advantages of multimodal NIR fluorescence/SERS for a better understanding of a plant’s response to stress,thereby improving the accuracy and sensitivity of detection.
基金financially supported by the Key Research&Development Program of Guangxi(No.GuiKeAB22080088)the Joint Project on Regional High-Incidence Diseases Research of Guangxi Natural Science Foundation(No.2023GXNSFDA026023)+3 种基金the Natural Science Foundation of Guangxi(No.2023JJA140322)the National Natural Science Foundation of China(No.82360372)the High-level Medical Expert Training Program of Guangxi“139 Plan Funding(No.G202003010)the Medical Appropriate Technology Development and Popularization and Application Project of Guangxi(No.S2020099)。
文摘Acute lung injury(ALI)was characterized by excessive reactive oxygen species(ROS)levels and inflammatory response in the lung.Scavenging ROS could inhibit the excessive inflammatory response,further treating ALI.Herein,we designed a novel nanozyme(P@Co)comprised of polydopamine(PDA)nanoparticles(NPs)loading with ultra-small Co,combining with near infrared(NIR)irradiation,which could efficiently scavenge intracellular ROS and suppress inflammatory responses against ALI.For lipopolysaccharide(LPS)induced macrophages,P@Co+NIR presented excellent antioxidant and anti-inflammatory capacities through lowering intracellular ROS levels,decreasing the expression levels of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)as well as inducing macrophage M2 directional polarization.Significantly,it displayed the outstanding activities of lowering acute lung inflammation,relieving diffuse alveolar damage,and up-regulating heat shock protein 70(HSP70)expression,resulting in synergistic enhanced ALI therapy effect.It offers a novel strategy for the clinical treatment of ROS related diseases.
基金supported by the National Natural Science Foundation of China(Nos.52173135,22207024)the Natural Science Foundation of Jiangsu Province(No.BK20231523)+5 种基金Jiangsu Specially Appointed Professorship,Leading Talents of Innovation and Entrepreneurship of Gusu(No.ZXL2022496)the Suzhou Science and Technology Program(No.SKY2022039)funded by the China Postdoctoral Science Foundation(Nos.2022M712305,2023M742536)the Jiangsu Funding Program for Excellent Postdoctoral Talent(No.2023ZB011)the project funded by the Priority Academic Program Development(PAPD)of Jiangsu Higher Education InstitutionsSuzhou International Joint Laboratory for Diagnosis and Treatment of Brain Diseases。
文摘Near-infrared(NIR)theranostics have received considerable attention because of their advantages in precise diagnostic imaging and efficient simultaneous treatment and have achieved tremendous advancements in the last few years.However,their progress is severely restricted by the rarity of efficient second NIR(NIR-Ⅱ)responsive phototheranostic materials,especially in the NIR-Ⅱb region.Moreover,these materials often embarrass the quenching puzzle in the aggregative state,thus greatly reducing their theranostic performance.To overcome this limitation,we developed anti-quenching donor-acceptor-donor(D-AD)-conjugated oligomers with NIR-Ⅱb emission for high-performance NIR-Ⅱangiography and phototheranostics.Through multi-acceptor engineering,a series of multi-acceptor conjugated oligomer SU-n(n=1,2,and 5)with tunable acceptor ratios were synthesized,and their efficiency in anti-quenching NIR-Ⅱemission was demonstrated.When prepared into water-dispersed nanoparticles(NPs),SU-5 NPs exhibit bright NIR-Ⅱemission and dual phototherapy for photothermal therapy and photodynamic therapy simultaneously upon 808 nm light excitation.With these benefits,high-resolution whole-body and local angiography in vivo of SU-5 NPs were successfully realized in the NIR-Ⅱb window.Moreover,in vivo,theranostics experiments demonstrated the efficiency of SU-5 NPs in NIR-Ⅱimaging-guided complete tumor photoablation without any relapses with high biosafety.This work explores a practical multi-acceptor engineering strategy for developing anti-quenching theranostic materials,providing an efficient theranostic agent for efficient NIR-Ⅱb bioimaging and phototheranostics.
基金supported by the Key Research and Development Program of Shaanxi(Program No.2023-YBSF-479)the National Natural Science Foundation of China(NSFC 22075249)the Fundamental Research Funds for the Central Universities.
文摘Rare earth luminescent materials have attracted extensive attention in the biomedical field as noncontact temperature monitoring devices with microscopic resolution due to their properties in the visible and near-infrared regions.At the application level,it is required to have a certain temperature monitoring capability in the near-infrared region II window to enhance the tissue penetration depth.Here,two kinds of YOFs:Er^(3+),Yb^(3+)were prepared by co-precipitation and hydrothermal method,and the luminescence was enhanced by ion doping.An Er^(3+)-based ratiometric nanothermometer of ^(4)F_(9∕2)→4 I_(15∕2)(672nm,upconversion luminescence)to ^(4)I _(13∕2)→^(4) I_(15∕2)(1580nm/1566nm,NIR II downshifting luminescence)were designed with the Stark energy level.When doped with 2%Zn^(2+),the relative temperature sensitivity of YOF prepared by co-precipitation method was improved from 0.30%℃^(-1)(30℃)to 0.59%℃^(-1)(30℃),expanding its use as a temperature monitoring device possibility.The temperature sensitivity of YOF prepared by hydrothermal method was 1.01%℃^(-1)(30℃).Finally,the NIR II luminescence of the prepared nanothermometer was used as a control for temperature monitoring of heating sites in mice.The results showed that it can distinguish heating site from control site and no significant cytotoxicity or damage to the tissues was revealed,indicating its broad prospects in the biomedical field and other temperature monitoring scenarios in the future.
