Objeclive The aim of this study was to investigate R-band of Cervus nippon hortulorum chromosomes and to provide references for genetic variation and gene location of Cervus nippon hortulorum. [Metbod] Cell division w...Objeclive The aim of this study was to investigate R-band of Cervus nippon hortulorum chromosomes and to provide references for genetic variation and gene location of Cervus nippon hortulorum. [Metbod] Cell division was synchronized by the pepripheral blood lymphocyte culture and the excessive dosage of thymine deoxyribonucleoside, and R-band of Cervus nippon hortulorum chromosomes was also analyzed by RBG-banding technique. Result The number of haploid chromosome banding increased to 400. The R-band of No. 1, No. 2, No. 3, No. 4, chromosome X and Y were almost just opposite to the high-resolution G band of them. The terminal of chromosomes except No. 21, No. 24 and No. 28 were all pos- itive deeply stained. E Conclusion] R-band of Cervus nippon hortulorum chromosomes can be manifested by RBG-binding technique.展开更多
The genetic diversity of 27 different Cervus nippon hortulorum was studied to provide theoretical evidence for their identification and utilization. The genomie DNA of 27 different Cereus nippon hortulorum were analyz...The genetic diversity of 27 different Cervus nippon hortulorum was studied to provide theoretical evidence for their identification and utilization. The genomie DNA of 27 different Cereus nippon hortulorum were analyzed by amplified fragment length polymorphism （AFLP）. 11,443 bands associated with genetic polymorphism among total 15,169 bands were obtained with 9 kinds of primer pairs and restriction endonuclease EcoRI/Msel, percentage of polymorphie band was 78.43%, 1,271 polymorphic locus were shown per primer pair. The AFLP data showed that average genetic similarity was 0.7841 （0.6809-0.8648）. 27 samples were classified into Group I and Group II with cluster analysis, and Group II was divided into five subgroups. The result of AFLP and cluster analysis concluded that there was high genetic variation, which associated with orientated artificial breed selection and breeding in the population. Genetic similarity of Group II-4 was the highest, more than 0.82, while genetic distance in this group was the shortest, from 0.1354 to 0.1563, which was coordinated with breeding record. All these showed that there was great genetic polymorphism among the deer population. The results laid the foundation for main quantitative trait locus （QTLs） of Cervus nippon hortulorum.展开更多
The Crested Ibis (Nipponia nippon) is a critical endangered species of the world. At present, a reintroduction program will be conducted to save this species essentially. However, because the Crested Ibise is a sexual...The Crested Ibis (Nipponia nippon) is a critical endangered species of the world. At present, a reintroduction program will be conducted to save this species essentially. However, because the Crested Ibise is a sexual alike bird, it is very difficult to identify the sex from the morphological character. In order to identify the sex easily and select the right individuals for captive breeding and reintroduction, the sex related gene on W chromosome was amplified and the sex of three Crested Ibises were also identified in the present study. The 262bp fragment was also sequenced, and we found that there were 13 different nucleotide sites with 2.25 of transition/transversion based on the comparison with that of the Oriental White Stork. The sequence will also provide a theoretic base for further designing specific primer for sex related gene in the Crested Ibis.展开更多
Bacterial infections of avian embryos can lead to an increase in embryo mortality,and the proliferation of antimicrobial-resistant bacteria aggravates the situation.A low hatching rate also poses a challenge to the po...Bacterial infections of avian embryos can lead to an increase in embryo mortality,and the proliferation of antimicrobial-resistant bacteria aggravates the situation.A low hatching rate also poses a challenge to the population of artificially bred Crested Ibises(Nipponia nippon).This study aims to determine the potential association between bacterial infection and the death of Crested Ibis embryos,and whether there is convergence between antimicrobial resistance and virulence in strain.In this study,13 Escherichia coli and 12 Proteus mirabilis isolates were recovered from dead Crested Ibis embryos.The pathogenicity examination confirmed the pathogenicity of all isolates,and multiple virulence genes detected by PCR-sequencing demonstrated the presence of irp2 and iuc D(100%),fim C and iss(92.31%)in E.coli,and uca A(58.33%)in P.mirabilis.Antimicrobial susceptibility test demonstrated that isolates were mainly resistant to amoxicillin(E.coli:76.92%,P.mirabilis:91.67%),cefazolin(E.coli:76.92%,P.mirabilis:91.67%),oxytetracycline(E.coli:92.31%,P.mirabilis:75.00%)and sulfamethoxazole-trimethoprim(E.coli:53.85%,P.mirabilis:33.33%),and more than 30%of isolates showed multidrug-resistance(MDR).Further analyses detected extended-spectrumβ-lactamase(ESBL)genes,of which blaTEM-1(E.coli:100%,P.mirabilis:100%)had the highest frequency,followed by the blaCTX-M-55(E.coli:92.31%,P.mirabilis:50%),blaCTX-M-14(E.coli:76.92%,P.mirabilis:33.33%),blaCTX-M-65(E.coli:15.38%,P.mirabilis:16.67%),and all isolates were negative for blaSHV and blaOXA.Pearson's correlation analysis showed a positive correlation between the presence ofβ-lactam resistance and ESBL genes,while mainly negative correlations were observed between the presence of ESBL genes and virulence genes.Furthermore,the conjugation experiment and PFGE revealed that the isolates were primarily polyclonal,and there was horizontal transfer of resistance or virulence genes by plasmids.Based on the results,E.coli and P.mirabilis were responsible for embryonic mortality of the ibises in this study.The co-presence and co-transfer of ESBL genes and virulence genes can pose a potential threat to the health of the Crested Ibis,and measures such as prudent use of antimicrobials,and constant surveillance of resistance and pathogenicity,must be implemented at the Crested Ibis breeding base.展开更多
基金supported by Chongqing Normal University Fund (XLY012)Natural Science Foundation of Chongqing Science and Technology Commission (CSTC 2006BB1260)
文摘Objeclive The aim of this study was to investigate R-band of Cervus nippon hortulorum chromosomes and to provide references for genetic variation and gene location of Cervus nippon hortulorum. [Metbod] Cell division was synchronized by the pepripheral blood lymphocyte culture and the excessive dosage of thymine deoxyribonucleoside, and R-band of Cervus nippon hortulorum chromosomes was also analyzed by RBG-banding technique. Result The number of haploid chromosome banding increased to 400. The R-band of No. 1, No. 2, No. 3, No. 4, chromosome X and Y were almost just opposite to the high-resolution G band of them. The terminal of chromosomes except No. 21, No. 24 and No. 28 were all pos- itive deeply stained. E Conclusion] R-band of Cervus nippon hortulorum chromosomes can be manifested by RBG-binding technique.
文摘The genetic diversity of 27 different Cervus nippon hortulorum was studied to provide theoretical evidence for their identification and utilization. The genomie DNA of 27 different Cereus nippon hortulorum were analyzed by amplified fragment length polymorphism （AFLP）. 11,443 bands associated with genetic polymorphism among total 15,169 bands were obtained with 9 kinds of primer pairs and restriction endonuclease EcoRI/Msel, percentage of polymorphie band was 78.43%, 1,271 polymorphic locus were shown per primer pair. The AFLP data showed that average genetic similarity was 0.7841 （0.6809-0.8648）. 27 samples were classified into Group I and Group II with cluster analysis, and Group II was divided into five subgroups. The result of AFLP and cluster analysis concluded that there was high genetic variation, which associated with orientated artificial breed selection and breeding in the population. Genetic similarity of Group II-4 was the highest, more than 0.82, while genetic distance in this group was the shortest, from 0.1354 to 0.1563, which was coordinated with breeding record. All these showed that there was great genetic polymorphism among the deer population. The results laid the foundation for main quantitative trait locus （QTLs） of Cervus nippon hortulorum.
基金the National Natural ScienceFoundation of China (Grant No. 39870098), the Science and Technology Innovation Program of the Chinese Academy of Sciences (CAS) (Grant No. KSCX2-1-03) and the Young Scientist Group of the CAS.
文摘The Crested Ibis (Nipponia nippon) is a critical endangered species of the world. At present, a reintroduction program will be conducted to save this species essentially. However, because the Crested Ibise is a sexual alike bird, it is very difficult to identify the sex from the morphological character. In order to identify the sex easily and select the right individuals for captive breeding and reintroduction, the sex related gene on W chromosome was amplified and the sex of three Crested Ibises were also identified in the present study. The 262bp fragment was also sequenced, and we found that there were 13 different nucleotide sites with 2.25 of transition/transversion based on the comparison with that of the Oriental White Stork. The sequence will also provide a theoretic base for further designing specific primer for sex related gene in the Crested Ibis.
基金supported by Research on Breeding and Healthy Breeding Technology of Xueyu White Chicken(mating line)in Tibet Science and Technology Program(XZ202101ZY0002N)the National Key R&D Program Project(2022YFD1600902-4)Sichuan Province Regional Innovation Cooperation Project(2023YFQ0050)。
文摘Bacterial infections of avian embryos can lead to an increase in embryo mortality,and the proliferation of antimicrobial-resistant bacteria aggravates the situation.A low hatching rate also poses a challenge to the population of artificially bred Crested Ibises(Nipponia nippon).This study aims to determine the potential association between bacterial infection and the death of Crested Ibis embryos,and whether there is convergence between antimicrobial resistance and virulence in strain.In this study,13 Escherichia coli and 12 Proteus mirabilis isolates were recovered from dead Crested Ibis embryos.The pathogenicity examination confirmed the pathogenicity of all isolates,and multiple virulence genes detected by PCR-sequencing demonstrated the presence of irp2 and iuc D(100%),fim C and iss(92.31%)in E.coli,and uca A(58.33%)in P.mirabilis.Antimicrobial susceptibility test demonstrated that isolates were mainly resistant to amoxicillin(E.coli:76.92%,P.mirabilis:91.67%),cefazolin(E.coli:76.92%,P.mirabilis:91.67%),oxytetracycline(E.coli:92.31%,P.mirabilis:75.00%)and sulfamethoxazole-trimethoprim(E.coli:53.85%,P.mirabilis:33.33%),and more than 30%of isolates showed multidrug-resistance(MDR).Further analyses detected extended-spectrumβ-lactamase(ESBL)genes,of which blaTEM-1(E.coli:100%,P.mirabilis:100%)had the highest frequency,followed by the blaCTX-M-55(E.coli:92.31%,P.mirabilis:50%),blaCTX-M-14(E.coli:76.92%,P.mirabilis:33.33%),blaCTX-M-65(E.coli:15.38%,P.mirabilis:16.67%),and all isolates were negative for blaSHV and blaOXA.Pearson's correlation analysis showed a positive correlation between the presence ofβ-lactam resistance and ESBL genes,while mainly negative correlations were observed between the presence of ESBL genes and virulence genes.Furthermore,the conjugation experiment and PFGE revealed that the isolates were primarily polyclonal,and there was horizontal transfer of resistance or virulence genes by plasmids.Based on the results,E.coli and P.mirabilis were responsible for embryonic mortality of the ibises in this study.The co-presence and co-transfer of ESBL genes and virulence genes can pose a potential threat to the health of the Crested Ibis,and measures such as prudent use of antimicrobials,and constant surveillance of resistance and pathogenicity,must be implemented at the Crested Ibis breeding base.
