BACKGROUND NIMA related kinase 2(NEK2) is closely related to mitosis, and it is currently considered to be over-expressed frequently in many poorly prognostic cancers.However, the effect of the up-regulated NEK2 on ce...BACKGROUND NIMA related kinase 2(NEK2) is closely related to mitosis, and it is currently considered to be over-expressed frequently in many poorly prognostic cancers.However, the effect of the up-regulated NEK2 on cellular signaling in tumors,such as gastric cancer(GC), is con-fusing.AIM To determine the role of the up-regulation of NEK2 in GC.METHODS To investigate the pathological significance of NEK2 in GC, the expression pattern of NEK2 in GC was investigated based on the 'Oncomain' database and compared between 30 pairs of cancer samples and adjacent tissues. The coexpression of NEK2 and ERK in GC was analyzed using The Cancer Genome Atlas(TCGA) database and confirmed in clinical samples by quantitative realtime PCR(qRT-PCR), and the survival curve was also plotted. Western blot or qRT-PCR was used to analyze the effect of NEK2 on the phosphorylation levels of ERK and c-JUN in two GC cell lines(BGC823 and SGC7901) with NEK2 overexpression, and the expression of the downstream effector cyclin D1.Furthermore, CCK8, EdU incorporation assay, and flow cytometry were used to detect the proliferative ability of BGC823 and SGC7901 cells with stably silenced ERK.RESULTS NEK2 was significantly up-regulated in human GC tissues. ERK was significantly associated with NEK2 expression in human clinical specimens, and combined overexpression of NEK2 and ERK potentially forecasted a poor prognosis andsurvival in GC patients. NEK2 knockdown in GC cells inhibited ERK and c-JUN phosphory-lation and reduced the transcription of cyclin D1. More interestingly,NEK2 can rescue the inhibition of cellular viability, proliferation, and cell cycle progression due to ERK knockdown.CONCLUSION Our results indicate that NEK2 plays a carcinogenic role in the malignant proliferation of GC cells via the ERK/MAPK signaling, which may be important for treatment and improving patient survival.展开更多
Lobsang Nima Soghatsang, a Tibetan who lives at the foot of the Alps in Switzerland, has lived overseas for more than 40 years. In the early Spring of 2001, he founded the "Lhonyima Nursing Home" in Garze of...Lobsang Nima Soghatsang, a Tibetan who lives at the foot of the Alps in Switzerland, has lived overseas for more than 40 years. In the early Spring of 2001, he founded the "Lhonyima Nursing Home" in Garze of Sichuan Province to adopt orphans, support poor children, and deliver medical care and medicines to villagers in remote areas. For several years, Lobsang Nima has managed to raise millions of Yuan for his cause. At the beginning of 2006, at the age of 65, he came back to China from Switzerland, to su...展开更多
Paleoenvironmental reconstruction plays a pivotal role in providing insights into the uplift history of the Xizang Plateau during the Cenozoic.The Nima Basin,situated in the central Xizang Plateau,is crucial for study...Paleoenvironmental reconstruction plays a pivotal role in providing insights into the uplift history of the Xizang Plateau during the Cenozoic.The Nima Basin,situated in the central Xizang Plateau,is crucial for studying the tectonic and geomorphic evolution of this region.The clastic composition and geochemical characteristics of the Niubao Formation hold considerable potential for unravelling the geological history and reconstructing depositional environments of central Xizang in the early Cenozoic.In this study,we present detailed geochemical characteristics to determine their provenance,paleoenvironmental conditions,and tectonic origins.The index of compositional variability(ICV>1)of mudstones indicates that low compositional maturity sediments underwent weak sedimentary recycling.The chemical index of alteration(CIA:59.8−72.9)reveals that parental rocks experienced a moderate chemical weathering degree.The paleoclimate indicators of the mudstones suggest an oxidizing and arid depositional environment,with a mean annual temperature(MAT)of 11.64℃±4.19℃.The geochemical evidence also demonstrates that the mudstones were derived from mixed felsic and intermediate igneous rocks that formed in a dominantly continental island arc tectonic setting.Similarities in the geochemical characteristics among the Niubao Formation and surrounding igneous rocks indicate that a continental-scale drainage system once drained westward in central Xizang.It is concluded that the central plateau experienced a cooler and drier climate coinciding with the presence of a large-scale drainage system during the late Eocene.展开更多
文摘BACKGROUND NIMA related kinase 2(NEK2) is closely related to mitosis, and it is currently considered to be over-expressed frequently in many poorly prognostic cancers.However, the effect of the up-regulated NEK2 on cellular signaling in tumors,such as gastric cancer(GC), is con-fusing.AIM To determine the role of the up-regulation of NEK2 in GC.METHODS To investigate the pathological significance of NEK2 in GC, the expression pattern of NEK2 in GC was investigated based on the 'Oncomain' database and compared between 30 pairs of cancer samples and adjacent tissues. The coexpression of NEK2 and ERK in GC was analyzed using The Cancer Genome Atlas(TCGA) database and confirmed in clinical samples by quantitative realtime PCR(qRT-PCR), and the survival curve was also plotted. Western blot or qRT-PCR was used to analyze the effect of NEK2 on the phosphorylation levels of ERK and c-JUN in two GC cell lines(BGC823 and SGC7901) with NEK2 overexpression, and the expression of the downstream effector cyclin D1.Furthermore, CCK8, EdU incorporation assay, and flow cytometry were used to detect the proliferative ability of BGC823 and SGC7901 cells with stably silenced ERK.RESULTS NEK2 was significantly up-regulated in human GC tissues. ERK was significantly associated with NEK2 expression in human clinical specimens, and combined overexpression of NEK2 and ERK potentially forecasted a poor prognosis andsurvival in GC patients. NEK2 knockdown in GC cells inhibited ERK and c-JUN phosphory-lation and reduced the transcription of cyclin D1. More interestingly,NEK2 can rescue the inhibition of cellular viability, proliferation, and cell cycle progression due to ERK knockdown.CONCLUSION Our results indicate that NEK2 plays a carcinogenic role in the malignant proliferation of GC cells via the ERK/MAPK signaling, which may be important for treatment and improving patient survival.
文摘Lobsang Nima Soghatsang, a Tibetan who lives at the foot of the Alps in Switzerland, has lived overseas for more than 40 years. In the early Spring of 2001, he founded the "Lhonyima Nursing Home" in Garze of Sichuan Province to adopt orphans, support poor children, and deliver medical care and medicines to villagers in remote areas. For several years, Lobsang Nima has managed to raise millions of Yuan for his cause. At the beginning of 2006, at the age of 65, he came back to China from Switzerland, to su...
基金supported by the Second Xizang Plateau Scientific Expedition Program(No.2019QZKK0803)National Natural Science Foundation of China(Grant Nos.42172113 and 41802124)+2 种基金Fundamental Research Funds for the Central Universities(No.265202138)“Deep-time Digital Earth”Science and Technology Leading Talents Team Funds for the Central Universities for the Frontiers Science Center for Deep-time Digital Earth,China University of Geosciences(Beijing)(No.2652023001)Key Laboratory of Sedimentary Basin and Oil and Gas Resources,Ministry of Natural Resources of the PRC(No.cdcgs2018006).
文摘Paleoenvironmental reconstruction plays a pivotal role in providing insights into the uplift history of the Xizang Plateau during the Cenozoic.The Nima Basin,situated in the central Xizang Plateau,is crucial for studying the tectonic and geomorphic evolution of this region.The clastic composition and geochemical characteristics of the Niubao Formation hold considerable potential for unravelling the geological history and reconstructing depositional environments of central Xizang in the early Cenozoic.In this study,we present detailed geochemical characteristics to determine their provenance,paleoenvironmental conditions,and tectonic origins.The index of compositional variability(ICV>1)of mudstones indicates that low compositional maturity sediments underwent weak sedimentary recycling.The chemical index of alteration(CIA:59.8−72.9)reveals that parental rocks experienced a moderate chemical weathering degree.The paleoclimate indicators of the mudstones suggest an oxidizing and arid depositional environment,with a mean annual temperature(MAT)of 11.64℃±4.19℃.The geochemical evidence also demonstrates that the mudstones were derived from mixed felsic and intermediate igneous rocks that formed in a dominantly continental island arc tectonic setting.Similarities in the geochemical characteristics among the Niubao Formation and surrounding igneous rocks indicate that a continental-scale drainage system once drained westward in central Xizang.It is concluded that the central plateau experienced a cooler and drier climate coinciding with the presence of a large-scale drainage system during the late Eocene.
