Objectives:Intravesical Bacillus Calmette-Guérin(BCG)therapy is a gold standard for patients with high-risk non-muscle invasive bladder cancer(NMIBC).Although a long-lasting therapeutic response is observed in mo...Objectives:Intravesical Bacillus Calmette-Guérin(BCG)therapy is a gold standard for patients with high-risk non-muscle invasive bladder cancer(NMIBC).Although a long-lasting therapeutic response is observed in most patients,BCG failure occurs in 30%–50%of patients and a progression to muscle-invasive disease is found in 10%–15%.Therefore,predicting high-risk patients who might not benefit from BCG treatment is critical.The purpose of this study was to identify,whether the presence of specific oncogenic mutations might be indicative of BCG treatment response.Methods:Nineteen high-grade NMIBC patients who received intravesical BCG were retrospectively enrolled and divided into“responders”and“non-responders”groups.Tissue samples from transurethral resection of bladder cancer were performed before starting therapy and were examined using a multigene sequencing panel.Results:Mutations in TP53,FGFR3,PIK3CA,KRAS,CTNNB1,ALK and DDR2 genes were detected.TP53 and FGFR3 were found to be the most frequently mutated genes in our cohort(31.6%and 26.3%,respectively),followed by PIK3CA(15.8%).In the BCG-responsive patient group,90%of samples were found to have mutated genes,with almost 50%of them showing mutations in tyrosine kinase receptors and CTNNB1 genes.On the other hand,in the BCG-unresponsive group,we found mutations in 44.4%of samples,mainly in TP53 gene.Conclusions:Our findings suggest that a Next-Generation Sequencing(NGS)multigene panel is useful in predicting BCG response in patients with NMIBC.展开更多
Objective To introduce the experience of professor YIN Ke-jing,a national senior and famous doctor of traditional Chinese medicine,in treatment of abdominal distension after abdominal surgery with warming-needle moxib...Objective To introduce the experience of professor YIN Ke-jing,a national senior and famous doctor of traditional Chinese medicine,in treatment of abdominal distension after abdominal surgery with warming-needle moxibustion at Gōngsūn（公孙 SP 4）.Methods Bilateral SP 4 were selected,and warming-needle moxibustion was adopted.The treatment was conducted for twice a day,and treatment for 3 days was considered as 1 course of treatment.The efficacy was evaluated after 2 courses.Results The total effective rate was 96.9% of the 32 patients after treatment and observation for 2 courses.Conclusion This therapeutic method was easy and simple with less acupoints selected and rapid onset of effect.With definite clinical efficacy,this method was worthy of being popularized.展开更多
文摘Objectives:Intravesical Bacillus Calmette-Guérin(BCG)therapy is a gold standard for patients with high-risk non-muscle invasive bladder cancer(NMIBC).Although a long-lasting therapeutic response is observed in most patients,BCG failure occurs in 30%–50%of patients and a progression to muscle-invasive disease is found in 10%–15%.Therefore,predicting high-risk patients who might not benefit from BCG treatment is critical.The purpose of this study was to identify,whether the presence of specific oncogenic mutations might be indicative of BCG treatment response.Methods:Nineteen high-grade NMIBC patients who received intravesical BCG were retrospectively enrolled and divided into“responders”and“non-responders”groups.Tissue samples from transurethral resection of bladder cancer were performed before starting therapy and were examined using a multigene sequencing panel.Results:Mutations in TP53,FGFR3,PIK3CA,KRAS,CTNNB1,ALK and DDR2 genes were detected.TP53 and FGFR3 were found to be the most frequently mutated genes in our cohort(31.6%and 26.3%,respectively),followed by PIK3CA(15.8%).In the BCG-responsive patient group,90%of samples were found to have mutated genes,with almost 50%of them showing mutations in tyrosine kinase receptors and CTNNB1 genes.On the other hand,in the BCG-unresponsive group,we found mutations in 44.4%of samples,mainly in TP53 gene.Conclusions:Our findings suggest that a Next-Generation Sequencing(NGS)multigene panel is useful in predicting BCG response in patients with NMIBC.
文摘Objective To introduce the experience of professor YIN Ke-jing,a national senior and famous doctor of traditional Chinese medicine,in treatment of abdominal distension after abdominal surgery with warming-needle moxibustion at Gōngsūn（公孙 SP 4）.Methods Bilateral SP 4 were selected,and warming-needle moxibustion was adopted.The treatment was conducted for twice a day,and treatment for 3 days was considered as 1 course of treatment.The efficacy was evaluated after 2 courses.Results The total effective rate was 96.9% of the 32 patients after treatment and observation for 2 courses.Conclusion This therapeutic method was easy and simple with less acupoints selected and rapid onset of effect.With definite clinical efficacy,this method was worthy of being popularized.
