目的研究NG2细胞在大鼠脊髓损伤白质内源性增殖及形态特征。方法成年SD雄性大鼠42只,随机平均分为模型组和假手术组。按课题组自行设计的方法制作脊髓压迫模型,假手术组仅暴露脊髓。分别于术后1、3、7d运用免疫组化检测脊髓内NG2细胞的...目的研究NG2细胞在大鼠脊髓损伤白质内源性增殖及形态特征。方法成年SD雄性大鼠42只,随机平均分为模型组和假手术组。按课题组自行设计的方法制作脊髓压迫模型,假手术组仅暴露脊髓。分别于术后1、3、7d运用免疫组化检测脊髓内NG2细胞的表达。采用Image Pro Plus6.0软件对NG2阳性细胞计数并测量其胞体面积和突起长度。结果伤后1d,NG2+细胞增多(30.17±11.08)/视野,至3d达到高峰(90.75±9.40)/视野,7d后下降(78.38±8.91)/视野,但仍多于假手术组(19.92±6.68)/视野(P<0.05)。在假手术组,NG2+细胞平均胞体面积为(205.67±10.80)μm2、平均突起长度为(22.92±1.24)μm,伤后1d,NG2+细胞胞体变小(128.25±32.06)μm2、突起变短(10.98±4.25)μm,3d后胞体变大(225.26±16.64)μm2、突起增长(18.63±2.26)μm(P<0.05),至7d变化不明显(P>0.05)。在脊髓压迫损伤后,可见许多胞体较小呈圆形、突起少或无的NG2+细胞集落。结论在脊髓压迫损伤一周内,NG2细胞增殖活跃,胞体渐大,突起变长,但仍短于正常。展开更多
NG2 cells are a novel distinct class of central nervous system（CNS） glial cells,characterized by the expression of the chondroitin sulfate proteoglycan NG2.They have been detected in a variety of human CNS diseases....NG2 cells are a novel distinct class of central nervous system（CNS） glial cells,characterized by the expression of the chondroitin sulfate proteoglycan NG2.They have been detected in a variety of human CNS diseases.As morphological,physiological and biomolecular studies of NG2 cells have been conducted,their roles have been gradually revealed.Research on cellular and molecular mechanisms in the pathophysiological state was built on the preliminary findings of their physiological functions;and in turn,this helps to clarify their physiological roles and leads to the identification of novel therapeutic targets.This review summarizes recent findings regarding the potential roles of NG2 cells in traumatic brain injury,multiple sclerosis,glioma,epilepsy,Alzheimer＇s disease and electroconvulsive therapy for depression.展开更多
文摘目的研究NG2细胞在大鼠脊髓损伤白质内源性增殖及形态特征。方法成年SD雄性大鼠42只,随机平均分为模型组和假手术组。按课题组自行设计的方法制作脊髓压迫模型,假手术组仅暴露脊髓。分别于术后1、3、7d运用免疫组化检测脊髓内NG2细胞的表达。采用Image Pro Plus6.0软件对NG2阳性细胞计数并测量其胞体面积和突起长度。结果伤后1d,NG2+细胞增多(30.17±11.08)/视野,至3d达到高峰(90.75±9.40)/视野,7d后下降(78.38±8.91)/视野,但仍多于假手术组(19.92±6.68)/视野(P<0.05)。在假手术组,NG2+细胞平均胞体面积为(205.67±10.80)μm2、平均突起长度为(22.92±1.24)μm,伤后1d,NG2+细胞胞体变小(128.25±32.06)μm2、突起变短(10.98±4.25)μm,3d后胞体变大(225.26±16.64)μm2、突起增长(18.63±2.26)μm(P<0.05),至7d变化不明显(P>0.05)。在脊髓压迫损伤后,可见许多胞体较小呈圆形、突起少或无的NG2+细胞集落。结论在脊髓压迫损伤一周内,NG2细胞增殖活跃,胞体渐大,突起变长,但仍短于正常。
基金supported by the National Natural Science Foundation of China(30871006)the Liaoning Educational Committee Foundation of China(2008168)the Scientific Research Foundation for Returned Overseas Chinese Scholars,Ministry of Education of China(2009)
文摘NG2 cells are a novel distinct class of central nervous system（CNS） glial cells,characterized by the expression of the chondroitin sulfate proteoglycan NG2.They have been detected in a variety of human CNS diseases.As morphological,physiological and biomolecular studies of NG2 cells have been conducted,their roles have been gradually revealed.Research on cellular and molecular mechanisms in the pathophysiological state was built on the preliminary findings of their physiological functions;and in turn,this helps to clarify their physiological roles and leads to the identification of novel therapeutic targets.This review summarizes recent findings regarding the potential roles of NG2 cells in traumatic brain injury,multiple sclerosis,glioma,epilepsy,Alzheimer＇s disease and electroconvulsive therapy for depression.
