Practitioners and researchers in the field of software engineering have realized that Non Functional requirement have not received due attention and second grade (or no) treatment has been meted out to Non Functional ...Practitioners and researchers in the field of software engineering have realized that Non Functional requirement have not received due attention and second grade (or no) treatment has been meted out to Non Functional Requirements. Many software products/systems are finally not acceptable because of such an approach. This casual approach of treating NFR has moved on to Testing also. Testing of NFR has never been taken seriously. Here in this work, we attempt to under- stand what needs to be done for proper consideration of NFR, so that they are treated as seriously as the Functional Requirements. In an attempt to treat NFR as seriously as FR we work on the testability of NFR by refining an abstract quality concern into concrete NFR statements. We show that quality concerns needs to be analyzed, for identifying and finally converting them into appropriate and unambiguous NFR. Once a high quality of NFR is ensured then the consequent testing of these NFRs will become as effective as that of Functional Requirement. We finally propose a revised model of Problem Analysis and Requirement Specification. A step wise refinement model for quality concern into test- able Non Functional Requirement is also proposed.展开更多
Chitin and its deacetylated derivative chitosan are the major components of fungal cell walls and are recognized by plant pattern-recognition receptors(PRRs)as pathogen-associated molecular patterns that induce innate...Chitin and its deacetylated derivative chitosan are the major components of fungal cell walls and are recognized by plant pattern-recognition receptors(PRRs)as pathogen-associated molecular patterns that induce innate immunity.Recognition of chitin oligosaccharide(CTOS)in Arabidopsis(Arabidopsis thaliana)and rice(Oryza sativa)requires the membrane-localized lysin-motif(LysM)-domain-containing receptors AtLYK5and OsCEBiP,respectively.However,the mechanism underlying chitosan oligosaccharide(CSOS)-induced plant immunity remains unclear.In this study,we determined that CTOS and CSOS trigger immune responses and boost disease resistance in soybean(Glycine max)through the LysM-domain-containing protein GmNRF5a and its co-receptor GmCERK1.Surprisingly,both GmNFR5a and GmCERK1 bind directly to CTOS and CSOS,with distinct binding sites.The receptor-like kinase GmCAK1 acts downstream of GmCERK1 and is essential for CTOS/CSOSmediated immune activation.Overall,these findings uncovered how soybean plants respond to CSOS and initiate immune signaling,demonstrating that soybean exploits shared immune sectors to transduce immune signals triggered by CTOS/CSOS,paving the way for the development of disease-resistant crops with broad-spectrum resistance.展开更多
文摘Practitioners and researchers in the field of software engineering have realized that Non Functional requirement have not received due attention and second grade (or no) treatment has been meted out to Non Functional Requirements. Many software products/systems are finally not acceptable because of such an approach. This casual approach of treating NFR has moved on to Testing also. Testing of NFR has never been taken seriously. Here in this work, we attempt to under- stand what needs to be done for proper consideration of NFR, so that they are treated as seriously as the Functional Requirements. In an attempt to treat NFR as seriously as FR we work on the testability of NFR by refining an abstract quality concern into concrete NFR statements. We show that quality concerns needs to be analyzed, for identifying and finally converting them into appropriate and unambiguous NFR. Once a high quality of NFR is ensured then the consequent testing of these NFRs will become as effective as that of Functional Requirement. We finally propose a revised model of Problem Analysis and Requirement Specification. A step wise refinement model for quality concern into test- able Non Functional Requirement is also proposed.
基金supported by the National Key Research and Development Program of China(Grant No.2022YFF1001500)the National Natural Science Foundation of China(Grant No.32102233 to G.S.,Grant No.31971217 to H.Y.)+3 种基金the China Postdoctoral Science Foundation(Grant No.2024T170421,G.S.)the Jiangsu Funding Program for Excellent Postdoctoral Talent(Grant No.2023ZB236,G.S.)the Postdoctoral Fellowship Program of CPSF(Grant No.GZC20231129,G.S.)the Key Research and Development Program of Guangdong Province(Grant No.2022B0202080004)。
文摘Chitin and its deacetylated derivative chitosan are the major components of fungal cell walls and are recognized by plant pattern-recognition receptors(PRRs)as pathogen-associated molecular patterns that induce innate immunity.Recognition of chitin oligosaccharide(CTOS)in Arabidopsis(Arabidopsis thaliana)and rice(Oryza sativa)requires the membrane-localized lysin-motif(LysM)-domain-containing receptors AtLYK5and OsCEBiP,respectively.However,the mechanism underlying chitosan oligosaccharide(CSOS)-induced plant immunity remains unclear.In this study,we determined that CTOS and CSOS trigger immune responses and boost disease resistance in soybean(Glycine max)through the LysM-domain-containing protein GmNRF5a and its co-receptor GmCERK1.Surprisingly,both GmNFR5a and GmCERK1 bind directly to CTOS and CSOS,with distinct binding sites.The receptor-like kinase GmCAK1 acts downstream of GmCERK1 and is essential for CTOS/CSOSmediated immune activation.Overall,these findings uncovered how soybean plants respond to CSOS and initiate immune signaling,demonstrating that soybean exploits shared immune sectors to transduce immune signals triggered by CTOS/CSOS,paving the way for the development of disease-resistant crops with broad-spectrum resistance.
