目的探索转录因子(NF-E2)相关因子3(NFE2L3)在直肠癌(RC)患者中的表达水平及其与临床预后指标的相关性。方法应用癌症相关数据库GEPIA和Oncomine 中NFE2L3基因的表达水平和预后行生物信息预测;回顾性分析66对RC与配对癌旁组织,行免疫组...目的探索转录因子(NF-E2)相关因子3(NFE2L3)在直肠癌(RC)患者中的表达水平及其与临床预后指标的相关性。方法应用癌症相关数据库GEPIA和Oncomine 中NFE2L3基因的表达水平和预后行生物信息预测;回顾性分析66对RC与配对癌旁组织,行免疫组化的方法检测NFE2L3基因蛋白表达;而后通过Kaplan-Meier单因素和Cox多因素分析研究NFE2L3表达水平与RC患者预后的关系。结果 GEPIA和Oncomine数据库均预测到NFE2L3基因mRNA水平在RC组织中显著高于正常组织( P <0.05),GEPIA数据库显示NFE2L3基因高表达的RC患者临床预后优于低表达者( P =0.041),且该结果与免疫组化结果相一致,RC组织中NFE2L3蛋白高表达( P <0.001),且临床预后优于低表达者( P =0.009)。多因素及单因素生存分析结果提示:NFE2L3蛋白表达水平是RC患者预后的独立预测因子。结论 NFE2L3基因在RC中高表达,可以作为预测RC患者预后良好的独立预测因子,有望成为未来RC患者治疗的新靶点。展开更多
Objective:To evaluate the antidiabetic effects of manool in streptozotocin-induced diabetic rats and HepG2 cells.Methods:Diabetes was induced in Wistar rats using streptozotocin and nicotinamide,and animals were treat...Objective:To evaluate the antidiabetic effects of manool in streptozotocin-induced diabetic rats and HepG2 cells.Methods:Diabetes was induced in Wistar rats using streptozotocin and nicotinamide,and animals were treated with two doses of manool(1 and 2 mg/kg).Biochemical,oxidative stress,apoptotic,and inflammatory parameters were assessed,followed by histopathology of the pancreas.Expression of key marker genes in the liver and pancreas was analyzed via qRT-PCR.Catalase and superoxide dismutase activities,malondialdehyde level,and glucose consumption were examined in vitro.Results:Manool significantly reduced fasting glucose level,improved insulin levels,and restored glucokinase and Ki67 expression in rats with diabetes.It also enhanced antioxidant defense,upregulated insulin signaling,activated the mTOR pathway,and promoted β-cell regeneration via increased expression of Pdx1,MAFA,Ngn3,and Ins1 in a dose-dependent manner.However,manool suppressed JAK/STAT pathway only at a higher dose of 2 mg/kg.Histopathological study showed near-normal islet architecture in manool-treated diabetic rats.Conclusions:Manool exerts antidiabetic effects by modulating oxidative stress,inflammation,β-cell regeneration,and insulin sensitivity in diabetic rats.However,further pharmacological and clinical investigations are required before confirming its therapeutic applicability.展开更多
Occupatio nal n oise is among the most comm on risks associated with the wellbeing of employees. Occupational exposure to noise causes disabling hearing loss in 16% of adults worldwide.It has bee n ack no wledged that...Occupatio nal n oise is among the most comm on risks associated with the wellbeing of employees. Occupational exposure to noise causes disabling hearing loss in 16% of adults worldwide.It has bee n ack no wledged that no ise-induced heari ng loss (NIHL) is a multifactorial disease, having both genetic and environ mental factors. NIHL continues to be permanent as well as irreversible, but NIHL can be preve nted. As dem on strated by the latest research, excessive oxidative stress in the cochlea has a close link with the pathogenesis of NIHL. which highlights the fact that appropriate control of oxidative stress is a productive strategy for preve nting the in crease in prevale nee and progression of NIHL.展开更多
文摘目的探索转录因子(NF-E2)相关因子3(NFE2L3)在直肠癌(RC)患者中的表达水平及其与临床预后指标的相关性。方法应用癌症相关数据库GEPIA和Oncomine 中NFE2L3基因的表达水平和预后行生物信息预测;回顾性分析66对RC与配对癌旁组织,行免疫组化的方法检测NFE2L3基因蛋白表达;而后通过Kaplan-Meier单因素和Cox多因素分析研究NFE2L3表达水平与RC患者预后的关系。结果 GEPIA和Oncomine数据库均预测到NFE2L3基因mRNA水平在RC组织中显著高于正常组织( P <0.05),GEPIA数据库显示NFE2L3基因高表达的RC患者临床预后优于低表达者( P =0.041),且该结果与免疫组化结果相一致,RC组织中NFE2L3蛋白高表达( P <0.001),且临床预后优于低表达者( P =0.009)。多因素及单因素生存分析结果提示:NFE2L3蛋白表达水平是RC患者预后的独立预测因子。结论 NFE2L3基因在RC中高表达,可以作为预测RC患者预后良好的独立预测因子,有望成为未来RC患者治疗的新靶点。
文摘Objective:To evaluate the antidiabetic effects of manool in streptozotocin-induced diabetic rats and HepG2 cells.Methods:Diabetes was induced in Wistar rats using streptozotocin and nicotinamide,and animals were treated with two doses of manool(1 and 2 mg/kg).Biochemical,oxidative stress,apoptotic,and inflammatory parameters were assessed,followed by histopathology of the pancreas.Expression of key marker genes in the liver and pancreas was analyzed via qRT-PCR.Catalase and superoxide dismutase activities,malondialdehyde level,and glucose consumption were examined in vitro.Results:Manool significantly reduced fasting glucose level,improved insulin levels,and restored glucokinase and Ki67 expression in rats with diabetes.It also enhanced antioxidant defense,upregulated insulin signaling,activated the mTOR pathway,and promoted β-cell regeneration via increased expression of Pdx1,MAFA,Ngn3,and Ins1 in a dose-dependent manner.However,manool suppressed JAK/STAT pathway only at a higher dose of 2 mg/kg.Histopathological study showed near-normal islet architecture in manool-treated diabetic rats.Conclusions:Manool exerts antidiabetic effects by modulating oxidative stress,inflammation,β-cell regeneration,and insulin sensitivity in diabetic rats.However,further pharmacological and clinical investigations are required before confirming its therapeutic applicability.
基金supported by Outstanding Medical Academic Leaders program of Jiangsu Province [LJ201130]Jiangsu Provincial Youth Medical Talent program [QNRC2016536]+2 种基金Six Talent Peaks Project in Jiangsu Province [WSW-017]Preventive Medicine Foundation of Jiangsu [Y2015049]The Fundamental Research Funds for the Central Universities [3225009404]
文摘Occupatio nal n oise is among the most comm on risks associated with the wellbeing of employees. Occupational exposure to noise causes disabling hearing loss in 16% of adults worldwide.It has bee n ack no wledged that no ise-induced heari ng loss (NIHL) is a multifactorial disease, having both genetic and environ mental factors. NIHL continues to be permanent as well as irreversible, but NIHL can be preve nted. As dem on strated by the latest research, excessive oxidative stress in the cochlea has a close link with the pathogenesis of NIHL. which highlights the fact that appropriate control of oxidative stress is a productive strategy for preve nting the in crease in prevale nee and progression of NIHL.
