Elucidating crops'physiological and molecular mechanisms to adapt to low nitrogen environment and promoting nitrogen transfer from senescent leaves to new leaves is crucial in improving Brassica's nitrogen use...Elucidating crops'physiological and molecular mechanisms to adapt to low nitrogen environment and promoting nitrogen transfer from senescent leaves to new leaves is crucial in improving Brassica's nitrogen use efficiency(NUE).Glutamine synthetase gene(GS)plays a vital role in helping plants reassimilate ammonium released from protein degradation in leaves,and it was the focus of our research on this topic.In this study,we identified high(H141)and low(L65)NUE genotypes of Brassica juncea with different responses to low-nitrogen stress.We found that H141 has a lower nitrate content but higher ammonium and free amino acid contents as well as higher nitrate reductase and GS activities in the shoots.These physiological indicators are responsible for the high NUE of H141.Wholegenome resequencing data revealed that 5,880 genes associated with NUE are polymorphic between H141 and L65.These genes participate in various amino acid,carbohydrate,and energy metabolic pathways.Haplotype analysis revealed two haplotypes for BjuB05.GS1.4,Hap1 and Hap2,which have multiple single nucleotide polymorphisms or insertions/deletions in the regulatory regions of the 5′and 3′untranslated regions and introns.Furthermore,the shoot NUE of Hap1 is significantly lower than that of Hap2.These two haplotypes of BjuB05.GS1.4 lead to differences in the shoot NUEs of different genetic populations of mustard and are associated with the local soil nitrogen content,suggesting that they might help mustard to adapt to different geographic localities.In conclusion,the results of our study shed light on the physiological and molecular mechanisms underlying different mustard NUE genotypes and demonstrate the enormous potential of NUE breeding in B.juncea.展开更多
BACKGROUND Hepatic stellate cell(HSC)activation is key to liver fibrosis.Targeting DNA methylation shows promise.Zebularine,a methylation inhibitor,may suppress HSC activation via the calcineurin(CaN)/NFAT3 pathway.Ma...BACKGROUND Hepatic stellate cell(HSC)activation is key to liver fibrosis.Targeting DNA methylation shows promise.Zebularine,a methylation inhibitor,may suppress HSC activation via the calcineurin(CaN)/NFAT3 pathway.Magnetic resonance imaging(MRI)is a noninvasive tool for evaluating liver fibrosis evaluation tool,but multiparametric MRI for zebularine’s effects in liver fibrosis mouse models has not been studied.AIM To clarify the anti-fibrosis mechanism and MRI-evaluated efficacy of zebularine.METHODS In vitro,transforming growth factor(TGF)-β1-stimulated human HSCs(LX-2)were treated with zebularine.α-smooth muscle actin,fibrotic and anti-fibrotic gene levels,and regulator of calcineurin1(RCAN1)regulation were measured.In vivo,carbon tetrachloride(CCl_(4))-induced liver fibrosis in mice was treated with zebularine,and fibrosis was evaluated using various biochemical,histopathological,and MRI methods.RESULTS Zebularine upregulated RCAN1.4 protein(P<0.01)and inhibited the CaN/NFAT3 pathway(P<0.05).In HSCs,TGF-β1 reduced anti-fibrotic gene massage RNA(mRNA)and increased fibrotic mRNA(P<0.05),whereas zebularine had the opposite effects(P<0.01,P<0.05).CCl4-treated mice exhibited increases in various fibrosis-related indices,all of which were reversed by zebularine treatment(P<0.05).CONCLUSION Zebularine may reduce LX-2 activation and extracellular matrix deposition via RCAN1.4 and CaN/NFAT3 path-ways.Multiparametric MRI can assess its efficacy,suggesting zebularine’s potential as a liver fibrosis treatment.展开更多
Composite solid electrolytes(CSEs)have emerged as promising candidates for safe and high-energy–density solid-state lithium metal batteries(SSLMBs).However,concurrently achieving exceptional ionic conductivity and in...Composite solid electrolytes(CSEs)have emerged as promising candidates for safe and high-energy–density solid-state lithium metal batteries(SSLMBs).However,concurrently achieving exceptional ionic conductivity and interface compatibility between the electrolyte and electrode presents a significant challenge in the development of high-performance CSEs for SSLMBs.To overcome these challenges,we present a method involving the in-situ polymerization of a monomer within a self-supported porous Li_(6.4)La_(3)Zr_(1.4)Ta_(0.6)O_(12)(LLZT)to produce the CSE.The synergy of the continuous conductive LLZT network,well-organized polymer,and their interface can enhance the ionic conductivity of the CSE at room temperature.Furthermore,the in-situ polymerization process can also con-struct the integration and compatibility of the solid electrolyte–solid electrode interface.The synthesized CSE exhibited a high ionic conductivity of 1.117 mS cm^(-1),a significant lithium transference number of 0.627,and exhibited electrochemical stability up to 5.06 V vs.Li/Li+at 30℃.Moreover,the Li|CSE|LiNi_(0.8)Co_(0.1)Mn_(0.1)O_(2) cell delivered a discharge capacity of 105.1 mAh g^(-1) after 400 cycles at 0.5 C and 30℃,corresponding to a capacity retention of 61%.This methodology could be extended to a variety of ceramic,polymer electrolytes,or battery systems,thereby offering a viable strategy to improve the electrochemical properties of CSEs for high-energy–density SSLMBs.展开更多
Garnet-type solid-state batteries(SSBs)are considered to be one of the most promising candidates to realize next-generation lithium metal batteries with high energy density and safety.However,the dendrite-induced shor...Garnet-type solid-state batteries(SSBs)are considered to be one of the most promising candidates to realize next-generation lithium metal batteries with high energy density and safety.However,the dendrite-induced short-circuit and the poor interfacial contact impeded the practical application.Herein,interface engineering to achieve low interfacial resistance without high temperature calcination was developed,which Li_(6.4)La_(3)Zr_(1.4)Ta_(0.6)O_(12)(LLZTO)was simply coated with complex hydride(Li_(4)(BH_(4))_(3)I(3L1L))in various mass ratios n(Li_(4)(BH_(4))_(3)I)-(100−n)LLZTO(10≤n≤40).The interfacial conductivity increases by more than three orders of magnitude from 8.29×10^(−6)S·cm^(−1)to 1.10×10^(−2)S·cm^(−1).Symmetric Li cells exhibit a high critical current density(CCD)of 4.0 mA·cm^(−2) and an excellent cycling stability for 200 h at 4.0 mA·cm^(−2).SSBs with polymeric sulfur-polyacrylonitrile(SPAN)cathode achieve a high discharge capacity of 1149 mAh·g^(−1) with a capacity retention of 91%after 100 cycles(0.2 C).This attempt guides a simple yet efficient strategy for obtaining a stable Li/LLZTO interface,which would promote the development of solid-state batteries.展开更多
Satisfactory ionic conductivity,excellent mechanical stability,and high-temperature resistance are the prerequisites for the safe application of solid polymer electrolytes(SPEs)in all-solid-state lithium metal batteri...Satisfactory ionic conductivity,excellent mechanical stability,and high-temperature resistance are the prerequisites for the safe application of solid polymer electrolytes(SPEs)in all-solid-state lithium metal batteries(ASSLMBs).In this study,a novel poly(m-phenylene isophthalamide)(PMIA)-core/poly(ethylene oxide)(PEO)-shell nanofiber membrane and the functional Li_(6.4)La_(3)Zr_(1.4)Ta_(0.6)O_(12)(LLZTO)ceramic nanopar-ticle are simultaneously introduced into the PEO-based SPEs to prepare composite polymer electrolytes(CPEs).The core PMIA layer of composite nanofibers can greatly improve the mechanical strength and thermal stability of the CPEs,while the shell PEO layer can provide the 3D continuous transport channels for lithium ions.In addition,the introduction of functional LLZTO nanoparticle not only reduces the crys-tallinity of PEO,but also promotes the dissociation of lithium salts and releases more Li^(+)ions through its interaction with the Lewis acid-base of anions,thereby overall improving the transport of lithium ions.Consequently,the optimized CPEs present high ionic conductivity of 1.38×10^(−4)S/cm at 30℃,signifi-cantly improved mechanical strength(8.5 MPa),remarkable thermal stability(without obvious shrinkage at 150℃),and conspicuous Li dendrites blocking ability(>1800 h).The CPEs also both have good com-patibility and cyclic stability with LiFePO_(4)(>2000 cycles)and high-voltage LiNi_(0.8)Mn_(0.1)Co_(0.1)O_(2)(NMC811)(>500 cycles)cathodes.In addition,even at low temperature(40℃),the assembled LiFePO4/CPEs/Li bat-tery still can cycle stably.The novel design can provide an effective way to exploit high-performance solid-state electrolytes.展开更多
基金supported by the National Natural Science Foundation of China(U21A20236,32072664)the Natural Science Foundation of Hunan Province,China(2022RC3053,2021JC0001,2021RC3086,2022NK2009)+1 种基金the China Agriculture Research System(CARS-01-30)the Innovation Foundation for Graduate of Hunan Agricultural University,China(2023XC116)。
文摘Elucidating crops'physiological and molecular mechanisms to adapt to low nitrogen environment and promoting nitrogen transfer from senescent leaves to new leaves is crucial in improving Brassica's nitrogen use efficiency(NUE).Glutamine synthetase gene(GS)plays a vital role in helping plants reassimilate ammonium released from protein degradation in leaves,and it was the focus of our research on this topic.In this study,we identified high(H141)and low(L65)NUE genotypes of Brassica juncea with different responses to low-nitrogen stress.We found that H141 has a lower nitrate content but higher ammonium and free amino acid contents as well as higher nitrate reductase and GS activities in the shoots.These physiological indicators are responsible for the high NUE of H141.Wholegenome resequencing data revealed that 5,880 genes associated with NUE are polymorphic between H141 and L65.These genes participate in various amino acid,carbohydrate,and energy metabolic pathways.Haplotype analysis revealed two haplotypes for BjuB05.GS1.4,Hap1 and Hap2,which have multiple single nucleotide polymorphisms or insertions/deletions in the regulatory regions of the 5′and 3′untranslated regions and introns.Furthermore,the shoot NUE of Hap1 is significantly lower than that of Hap2.These two haplotypes of BjuB05.GS1.4 lead to differences in the shoot NUEs of different genetic populations of mustard and are associated with the local soil nitrogen content,suggesting that they might help mustard to adapt to different geographic localities.In conclusion,the results of our study shed light on the physiological and molecular mechanisms underlying different mustard NUE genotypes and demonstrate the enormous potential of NUE breeding in B.juncea.
基金Supported by the Health Research Foundation of Hunan Provincial Health Commission,No.W20243192Natural Science Foundation of Changsha,No.kq2403086+1 种基金National Natural Science Foundation of China,No.81571784Hunan Provincial Health Commission Hunan Provincial High-level Health Talent Major Scientific Research Project,No.R2023022.
文摘BACKGROUND Hepatic stellate cell(HSC)activation is key to liver fibrosis.Targeting DNA methylation shows promise.Zebularine,a methylation inhibitor,may suppress HSC activation via the calcineurin(CaN)/NFAT3 pathway.Magnetic resonance imaging(MRI)is a noninvasive tool for evaluating liver fibrosis evaluation tool,but multiparametric MRI for zebularine’s effects in liver fibrosis mouse models has not been studied.AIM To clarify the anti-fibrosis mechanism and MRI-evaluated efficacy of zebularine.METHODS In vitro,transforming growth factor(TGF)-β1-stimulated human HSCs(LX-2)were treated with zebularine.α-smooth muscle actin,fibrotic and anti-fibrotic gene levels,and regulator of calcineurin1(RCAN1)regulation were measured.In vivo,carbon tetrachloride(CCl_(4))-induced liver fibrosis in mice was treated with zebularine,and fibrosis was evaluated using various biochemical,histopathological,and MRI methods.RESULTS Zebularine upregulated RCAN1.4 protein(P<0.01)and inhibited the CaN/NFAT3 pathway(P<0.05).In HSCs,TGF-β1 reduced anti-fibrotic gene massage RNA(mRNA)and increased fibrotic mRNA(P<0.05),whereas zebularine had the opposite effects(P<0.01,P<0.05).CCl4-treated mice exhibited increases in various fibrosis-related indices,all of which were reversed by zebularine treatment(P<0.05).CONCLUSION Zebularine may reduce LX-2 activation and extracellular matrix deposition via RCAN1.4 and CaN/NFAT3 path-ways.Multiparametric MRI can assess its efficacy,suggesting zebularine’s potential as a liver fibrosis treatment.
基金supported by the National Research Foundation of Korea (NRF) grant funded by the MSIT,Korea (No. 2018R1A5A1025224 and No. 2019R1A2C1084020)this research received funding support from a grant from the Korea Planning&Evaluation Institute of Industrial Technology (KEIT),funded by the MOTIE of Korea (No. 10077287)。
文摘Composite solid electrolytes(CSEs)have emerged as promising candidates for safe and high-energy–density solid-state lithium metal batteries(SSLMBs).However,concurrently achieving exceptional ionic conductivity and interface compatibility between the electrolyte and electrode presents a significant challenge in the development of high-performance CSEs for SSLMBs.To overcome these challenges,we present a method involving the in-situ polymerization of a monomer within a self-supported porous Li_(6.4)La_(3)Zr_(1.4)Ta_(0.6)O_(12)(LLZT)to produce the CSE.The synergy of the continuous conductive LLZT network,well-organized polymer,and their interface can enhance the ionic conductivity of the CSE at room temperature.Furthermore,the in-situ polymerization process can also con-struct the integration and compatibility of the solid electrolyte–solid electrode interface.The synthesized CSE exhibited a high ionic conductivity of 1.117 mS cm^(-1),a significant lithium transference number of 0.627,and exhibited electrochemical stability up to 5.06 V vs.Li/Li+at 30℃.Moreover,the Li|CSE|LiNi_(0.8)Co_(0.1)Mn_(0.1)O_(2) cell delivered a discharge capacity of 105.1 mAh g^(-1) after 400 cycles at 0.5 C and 30℃,corresponding to a capacity retention of 61%.This methodology could be extended to a variety of ceramic,polymer electrolytes,or battery systems,thereby offering a viable strategy to improve the electrochemical properties of CSEs for high-energy–density SSLMBs.
基金This study was financially supported by the National Natural Science Foundation of China(Nos.52171180,51802154,and 51971065)the National Science Fund for Distinguished Young Scholars(No.51625102)+3 种基金the Innovation Program of Shanghai Municipal Education Commission(No.2019-01-07-00-07-E00028)the Fundamental Research Funds for the Central Universities(No.NG2022005)the Open Fund for Graduate Innovation Base in Nanjing University of Aeronautics and Astronautics(No.xcxjh20210612)partially supported by the Fundamental Research Funds for the Central Universities,NS2021043.
文摘Garnet-type solid-state batteries(SSBs)are considered to be one of the most promising candidates to realize next-generation lithium metal batteries with high energy density and safety.However,the dendrite-induced short-circuit and the poor interfacial contact impeded the practical application.Herein,interface engineering to achieve low interfacial resistance without high temperature calcination was developed,which Li_(6.4)La_(3)Zr_(1.4)Ta_(0.6)O_(12)(LLZTO)was simply coated with complex hydride(Li_(4)(BH_(4))_(3)I(3L1L))in various mass ratios n(Li_(4)(BH_(4))_(3)I)-(100−n)LLZTO(10≤n≤40).The interfacial conductivity increases by more than three orders of magnitude from 8.29×10^(−6)S·cm^(−1)to 1.10×10^(−2)S·cm^(−1).Symmetric Li cells exhibit a high critical current density(CCD)of 4.0 mA·cm^(−2) and an excellent cycling stability for 200 h at 4.0 mA·cm^(−2).SSBs with polymeric sulfur-polyacrylonitrile(SPAN)cathode achieve a high discharge capacity of 1149 mAh·g^(−1) with a capacity retention of 91%after 100 cycles(0.2 C).This attempt guides a simple yet efficient strategy for obtaining a stable Li/LLZTO interface,which would promote the development of solid-state batteries.
基金supported by the National Natural Science Foundation of China (Nos.52203066,51973157,61904123)the Tianjin Natural Science Foundation (No.18JCQNJC02900)+3 种基金National Innovation and Entrepreneurship Training Program for College students (No.202310058007)Tianjin Municipal College Students’ Innovation and Entrepreneurship Training Program (No.202310058088)Science & Technology Development Fund of Tianjin Education Commission for Higher Education (No.2018KJ196)State Key Laboratory of Membrane and Membrane Separation,Tiangong University
文摘Satisfactory ionic conductivity,excellent mechanical stability,and high-temperature resistance are the prerequisites for the safe application of solid polymer electrolytes(SPEs)in all-solid-state lithium metal batteries(ASSLMBs).In this study,a novel poly(m-phenylene isophthalamide)(PMIA)-core/poly(ethylene oxide)(PEO)-shell nanofiber membrane and the functional Li_(6.4)La_(3)Zr_(1.4)Ta_(0.6)O_(12)(LLZTO)ceramic nanopar-ticle are simultaneously introduced into the PEO-based SPEs to prepare composite polymer electrolytes(CPEs).The core PMIA layer of composite nanofibers can greatly improve the mechanical strength and thermal stability of the CPEs,while the shell PEO layer can provide the 3D continuous transport channels for lithium ions.In addition,the introduction of functional LLZTO nanoparticle not only reduces the crys-tallinity of PEO,but also promotes the dissociation of lithium salts and releases more Li^(+)ions through its interaction with the Lewis acid-base of anions,thereby overall improving the transport of lithium ions.Consequently,the optimized CPEs present high ionic conductivity of 1.38×10^(−4)S/cm at 30℃,signifi-cantly improved mechanical strength(8.5 MPa),remarkable thermal stability(without obvious shrinkage at 150℃),and conspicuous Li dendrites blocking ability(>1800 h).The CPEs also both have good com-patibility and cyclic stability with LiFePO_(4)(>2000 cycles)and high-voltage LiNi_(0.8)Mn_(0.1)Co_(0.1)O_(2)(NMC811)(>500 cycles)cathodes.In addition,even at low temperature(40℃),the assembled LiFePO4/CPEs/Li bat-tery still can cycle stably.The novel design can provide an effective way to exploit high-performance solid-state electrolytes.
