目的探讨NAT10在肝癌中的临床意义与潜在作用机制。方法基于癌症基因组图谱(The Cancer Genome Atlas,TCGA)获得的50例正常和374例肿瘤样本,采用R 4.2.1处理所获数据。结果NAT10在肿瘤样本中的表达显著高于正常样本(P<0.001)。与NAT1...目的探讨NAT10在肝癌中的临床意义与潜在作用机制。方法基于癌症基因组图谱(The Cancer Genome Atlas,TCGA)获得的50例正常和374例肿瘤样本,采用R 4.2.1处理所获数据。结果NAT10在肿瘤样本中的表达显著高于正常样本(P<0.001)。与NAT10低表达患者比较,NAT10高表达患者预后较差(P<0.001)。Logistic回归分析结果表明,NAT10高表达与肝癌患者临床预后不良因素相关,NAT10高表达患者更易于进展到晚期。基因集富集分析(gene set enrichment analysis,GSEA)结果显示,NAT10高表达样本中,异生物质代谢、凝血、脂肪酸代谢等相关基因特征均有差异富集。蛋白互作分析结果显示,NAT10可能与IGF2、SST、MUC2、CHGA、AGR2等基因存在相互作用。结论NAT10在肝癌中高表达,且表达水平与肝癌的临床特征及患者生存率存在相关性。NAT10可能是一种潜在的肝癌预后生物学标志物。展开更多
Sodium metal batteries(SMBs)are promising candidates for next-generation energy storage devices owing to their excellent safety performance and natural abunda nce of sodium.However,the insurmountable obstacles of dend...Sodium metal batteries(SMBs)are promising candidates for next-generation energy storage devices owing to their excellent safety performance and natural abunda nce of sodium.However,the insurmountable obstacles of dendrite formation and quick capacity decay are caused by an unstable and inhomogeneous solid electrolyte interphase that resulted from the immediate interactions between the Na metal anode and organic liquid electrolyte.Herein,a customised glass fibre separator coupled with chitosan(CS@GF)was developed to modulate the sodium ion(Na^(+))flux.The CS@GF separator facilitates the Na+homogeneous deposition on the anode side through redistribution at the chitosan polyactive sites and by inhibiting the decomposition of the electrolyte to robust solid electrolyte interphase(SEI)formation.Multiphysics simulations show that chitosan incorporated into SMBs through the separator can make the local electric field around the anode uniform,thus facilitating the transfer of cations.Na|Na symmetric cells utilising a CS@GF separator exhibited an outstanding cycle stability of over 600 h(0.5 mA cm^(-2)).Meanwhile,the Na|Na_(3)V_(5)(PO_(4))_(3)full cell exhibited excellent fast-charging performance(93.47%capacity retention after 1500 cycles at 5C).This study presents a promising strategy for inhibiting dendrite growth and realizes stable Na metal batteries,which significantly boosts the development of high-performance SMBs.展开更多
Background:Neoadjuvant/preoperative therapy(NAT)involves the administration of chemotherapy,with or without radiation,prior to surgical resection.This approach is commonly used for locally advanced tumors to reduce tu...Background:Neoadjuvant/preoperative therapy(NAT)involves the administration of chemotherapy,with or without radiation,prior to surgical resection.This approach is commonly used for locally advanced tumors to reduce tumor volume,improve resectability,and minimize the need for extensive surgical procedures.While NAT has been shown to be effective in inducing local anti-tumor immunity in potentially resectable solid tumors,the underlying molecular mechanisms remain poorly understood.Methods:Cohort samples from pancreatic cancer patients who underwent NAT(n=26)and those who did not(n=20)were analyzed.Changes in the immune microenvironment induced by NAT were assessed using stratified bioinformatic approaches,including heatmap analysis of immunerelated genes selected via Gene Ontology,Gene Set Enrichment Analysis(GSEA)with the immunologic signature database,and Ingenuity Pathway Analysis(IPA).Findings were further validated through immunohistochemical analysis.Results:A comprehensive,stratified evaluation integrating pathological and bioinformatic approaches revealed that NAT induced the upregulation of 212 genes,including DC-SIGN(CD209),and activated 13 immuneassociated pathways,such as T-cell receptor(TCR)signaling.Additionally,NAT promoted an increased shift toward CD8(+)T-cell populations through the upregulation of MAL(T-cell differentiation protein).Immunohistochemical analysis further confirmed a significant accumulation of DC-SIGN(+)dendritic cells and MAL(+)lymphocytes in NAT-treated patients.Conclusions:NAT enhances anti-tumor immunity by promoting CD8(+)T-cell generation through the activation of DC-SIGN(+)dendritic cells and MAL(+)lymphocytes.This study is the first to report an increase in MAL(+)lymphocytes following NAT.Given its potential significance,further investigation in other solid tumors treated with NAT is warranted.展开更多
Lead(Pb)plays a significant role in the nuclear industry and is extensively used in radiation shielding,radiation protection,neutron moderation,radiation measurements,and various other critical functions.Consequently,...Lead(Pb)plays a significant role in the nuclear industry and is extensively used in radiation shielding,radiation protection,neutron moderation,radiation measurements,and various other critical functions.Consequently,the measurement and evaluation of Pb nuclear data are highly regarded in nuclear scientific research,emphasizing its crucial role in the field.Using the time-of-flight(ToF)method,the neutron leakage spectra from three^(nat)Pb samples were measured at 60°and 120°based on the neutronics integral experimental facility at the China Institute of Atomic Energy(CIAE).The^(nat)Pb sample sizes were30 cm×30 cm×5 cm,30 cm×30 cm×10 cm,and 30 cm×30 cm×15 cm.Neutron sources were generated by the Cockcroft-Walton accelerator,producing approximately 14.5 MeV and 3.5 MeV neutrons through the T(d,n)^(4)He and D(d,n)^(3)He reactions,respectively.Leakage neutron spectra were also calculated by employing the Monte Carlo code of MCNP-4C,and the nuclear data of Pb isotopes from four libraries:CENDL-3.2,JEFF-3.3,JENDL-5,and ENDF/B-Ⅷ.0 were used individually.By comparing the simulation and experimental results,improvements and deficiencies in the evaluated nuclear data of the Pb isotopes were analyzed.Most of the calculated results were consistent with the experimental results;however,a few areas did not fit well.In the(n,el)energy range,the simulated results from CENDL-3.2 were significantly overestimated;in the(n,inl)D and the(n,inl)C energy regions,the results from CENDL-3.2 and ENDF/B-Ⅷ.0 were significantly overestimated at 120°,and the results from JENDL-5 and JEFF-3.3 are underestimated at 60°in the(n,inl)D energy region.The calculated spectra were analyzed by comparing them with the experimental spectra in terms of the neutron spectrum shape and C/E values.The results indicate that the theoretical simulations,using different data libraries,overestimated or underestimated the measured values in certain energy ranges.Secondary neutron energies and angular distributions in the data files have been presented to explain these discrepancies.展开更多
Introduction The accuracy of sentinel lymph node biopsy(SLNB)after neoadjuvant therapy(NAT)has been confirmed in clinical nodal stage 1(c N1)patients,and more patients could benefit from axillary surgery de-escalation...Introduction The accuracy of sentinel lymph node biopsy(SLNB)after neoadjuvant therapy(NAT)has been confirmed in clinical nodal stage 1(c N1)patients,and more patients could benefit from axillary surgery de-escalation after NAT(1,2).展开更多
文摘目的探讨NAT10在肝癌中的临床意义与潜在作用机制。方法基于癌症基因组图谱(The Cancer Genome Atlas,TCGA)获得的50例正常和374例肿瘤样本,采用R 4.2.1处理所获数据。结果NAT10在肿瘤样本中的表达显著高于正常样本(P<0.001)。与NAT10低表达患者比较,NAT10高表达患者预后较差(P<0.001)。Logistic回归分析结果表明,NAT10高表达与肝癌患者临床预后不良因素相关,NAT10高表达患者更易于进展到晚期。基因集富集分析(gene set enrichment analysis,GSEA)结果显示,NAT10高表达样本中,异生物质代谢、凝血、脂肪酸代谢等相关基因特征均有差异富集。蛋白互作分析结果显示,NAT10可能与IGF2、SST、MUC2、CHGA、AGR2等基因存在相互作用。结论NAT10在肝癌中高表达,且表达水平与肝癌的临床特征及患者生存率存在相关性。NAT10可能是一种潜在的肝癌预后生物学标志物。
基金funded by the Key Research and Development Program of Shandong Province(2023CXPT069)Opening Funds of the State Key Laboratory of Building Safety and Built Environment(BSBE2022-EET-06)Innovation Project of Guangwei Group Academician Workstation(GWYS-2022-04)。
文摘Sodium metal batteries(SMBs)are promising candidates for next-generation energy storage devices owing to their excellent safety performance and natural abunda nce of sodium.However,the insurmountable obstacles of dendrite formation and quick capacity decay are caused by an unstable and inhomogeneous solid electrolyte interphase that resulted from the immediate interactions between the Na metal anode and organic liquid electrolyte.Herein,a customised glass fibre separator coupled with chitosan(CS@GF)was developed to modulate the sodium ion(Na^(+))flux.The CS@GF separator facilitates the Na+homogeneous deposition on the anode side through redistribution at the chitosan polyactive sites and by inhibiting the decomposition of the electrolyte to robust solid electrolyte interphase(SEI)formation.Multiphysics simulations show that chitosan incorporated into SMBs through the separator can make the local electric field around the anode uniform,thus facilitating the transfer of cations.Na|Na symmetric cells utilising a CS@GF separator exhibited an outstanding cycle stability of over 600 h(0.5 mA cm^(-2)).Meanwhile,the Na|Na_(3)V_(5)(PO_(4))_(3)full cell exhibited excellent fast-charging performance(93.47%capacity retention after 1500 cycles at 5C).This study presents a promising strategy for inhibiting dendrite growth and realizes stable Na metal batteries,which significantly boosts the development of high-performance SMBs.
基金supported by JSPS KAKENHI,grant number 18K16377(KN).
文摘Background:Neoadjuvant/preoperative therapy(NAT)involves the administration of chemotherapy,with or without radiation,prior to surgical resection.This approach is commonly used for locally advanced tumors to reduce tumor volume,improve resectability,and minimize the need for extensive surgical procedures.While NAT has been shown to be effective in inducing local anti-tumor immunity in potentially resectable solid tumors,the underlying molecular mechanisms remain poorly understood.Methods:Cohort samples from pancreatic cancer patients who underwent NAT(n=26)and those who did not(n=20)were analyzed.Changes in the immune microenvironment induced by NAT were assessed using stratified bioinformatic approaches,including heatmap analysis of immunerelated genes selected via Gene Ontology,Gene Set Enrichment Analysis(GSEA)with the immunologic signature database,and Ingenuity Pathway Analysis(IPA).Findings were further validated through immunohistochemical analysis.Results:A comprehensive,stratified evaluation integrating pathological and bioinformatic approaches revealed that NAT induced the upregulation of 212 genes,including DC-SIGN(CD209),and activated 13 immuneassociated pathways,such as T-cell receptor(TCR)signaling.Additionally,NAT promoted an increased shift toward CD8(+)T-cell populations through the upregulation of MAL(T-cell differentiation protein).Immunohistochemical analysis further confirmed a significant accumulation of DC-SIGN(+)dendritic cells and MAL(+)lymphocytes in NAT-treated patients.Conclusions:NAT enhances anti-tumor immunity by promoting CD8(+)T-cell generation through the activation of DC-SIGN(+)dendritic cells and MAL(+)lymphocytes.This study is the first to report an increase in MAL(+)lymphocytes following NAT.Given its potential significance,further investigation in other solid tumors treated with NAT is warranted.
基金supported by the National Natural Science Foundation of China(Nos.11775311 and U2067205)the Stable Support Basic Research Program Grant(BJ010261223282)the Research and Development Project of China National Nuclear Corporation。
文摘Lead(Pb)plays a significant role in the nuclear industry and is extensively used in radiation shielding,radiation protection,neutron moderation,radiation measurements,and various other critical functions.Consequently,the measurement and evaluation of Pb nuclear data are highly regarded in nuclear scientific research,emphasizing its crucial role in the field.Using the time-of-flight(ToF)method,the neutron leakage spectra from three^(nat)Pb samples were measured at 60°and 120°based on the neutronics integral experimental facility at the China Institute of Atomic Energy(CIAE).The^(nat)Pb sample sizes were30 cm×30 cm×5 cm,30 cm×30 cm×10 cm,and 30 cm×30 cm×15 cm.Neutron sources were generated by the Cockcroft-Walton accelerator,producing approximately 14.5 MeV and 3.5 MeV neutrons through the T(d,n)^(4)He and D(d,n)^(3)He reactions,respectively.Leakage neutron spectra were also calculated by employing the Monte Carlo code of MCNP-4C,and the nuclear data of Pb isotopes from four libraries:CENDL-3.2,JEFF-3.3,JENDL-5,and ENDF/B-Ⅷ.0 were used individually.By comparing the simulation and experimental results,improvements and deficiencies in the evaluated nuclear data of the Pb isotopes were analyzed.Most of the calculated results were consistent with the experimental results;however,a few areas did not fit well.In the(n,el)energy range,the simulated results from CENDL-3.2 were significantly overestimated;in the(n,inl)D and the(n,inl)C energy regions,the results from CENDL-3.2 and ENDF/B-Ⅷ.0 were significantly overestimated at 120°,and the results from JENDL-5 and JEFF-3.3 are underestimated at 60°in the(n,inl)D energy region.The calculated spectra were analyzed by comparing them with the experimental spectra in terms of the neutron spectrum shape and C/E values.The results indicate that the theoretical simulations,using different data libraries,overestimated or underestimated the measured values in certain energy ranges.Secondary neutron energies and angular distributions in the data files have been presented to explain these discrepancies.
文摘Introduction The accuracy of sentinel lymph node biopsy(SLNB)after neoadjuvant therapy(NAT)has been confirmed in clinical nodal stage 1(c N1)patients,and more patients could benefit from axillary surgery de-escalation after NAT(1,2).
