目的探讨NAT10在肝癌中的临床意义与潜在作用机制。方法基于癌症基因组图谱(The Cancer Genome Atlas,TCGA)获得的50例正常和374例肿瘤样本,采用R 4.2.1处理所获数据。结果NAT10在肿瘤样本中的表达显著高于正常样本(P<0.001)。与NAT1...目的探讨NAT10在肝癌中的临床意义与潜在作用机制。方法基于癌症基因组图谱(The Cancer Genome Atlas,TCGA)获得的50例正常和374例肿瘤样本,采用R 4.2.1处理所获数据。结果NAT10在肿瘤样本中的表达显著高于正常样本(P<0.001)。与NAT10低表达患者比较,NAT10高表达患者预后较差(P<0.001)。Logistic回归分析结果表明,NAT10高表达与肝癌患者临床预后不良因素相关,NAT10高表达患者更易于进展到晚期。基因集富集分析(gene set enrichment analysis,GSEA)结果显示,NAT10高表达样本中,异生物质代谢、凝血、脂肪酸代谢等相关基因特征均有差异富集。蛋白互作分析结果显示,NAT10可能与IGF2、SST、MUC2、CHGA、AGR2等基因存在相互作用。结论NAT10在肝癌中高表达,且表达水平与肝癌的临床特征及患者生存率存在相关性。NAT10可能是一种潜在的肝癌预后生物学标志物。展开更多
目的通过检测结核病患者药物代谢酶N-乙酰基转移酶2(N-acetyltransferase 2,NAT2)与妊娠X受体基因(Pregnane X receptor,PXR)启动子区rs3814055的基因多态性,根据结果进行风险分型,并验证对发生结核药物性肝损伤的预测价值。方法将382...目的通过检测结核病患者药物代谢酶N-乙酰基转移酶2(N-acetyltransferase 2,NAT2)与妊娠X受体基因(Pregnane X receptor,PXR)启动子区rs3814055的基因多态性,根据结果进行风险分型,并验证对发生结核药物性肝损伤的预测价值。方法将382例采用一线HREZ抗结核方案的敏感性结核患者在治疗前对NAT2及rs3814055的多态性位点进行测序分析,并进行风险分型,按结果分为高、中、低风险组,通过观察其实际发生肝损伤情况进行对比分析,以此验证风险类型与ATDILT间的相关性。结果低风险组患者185名,发生ATDILT的比例为15.7%(29/185);中风险组患者132名,发生ATDILT的比例为28.0%(37/132);高风险组患者65名,发生ATDILT的比例为32.3%(21/65)。高风险组与低风险组相比,发生肝损伤的相对危险度为1.909(95%CI为1.258~2.898);中风险组与低风险组相比,发生肝损伤的相对危险度为1.481(95%CI为1.135~1.933),且均有统计学意义(χ^(2)=8.316,P<0.01;χ^(2)=7.133,P<0.01)。高、中风险组间无显著性差异。结果显示患者性别与年龄对ATDILT发生率无显著性影响。结论基于NAT2及rs3814055多态性的联合检测的风险分型可用来预测结核病患者药物性肝损伤的发生。展开更多
Lead(Pb)plays a significant role in the nuclear industry and is extensively used in radiation shielding,radiation protection,neutron moderation,radiation measurements,and various other critical functions.Consequently,...Lead(Pb)plays a significant role in the nuclear industry and is extensively used in radiation shielding,radiation protection,neutron moderation,radiation measurements,and various other critical functions.Consequently,the measurement and evaluation of Pb nuclear data are highly regarded in nuclear scientific research,emphasizing its crucial role in the field.Using the time-of-flight(ToF)method,the neutron leakage spectra from three^(nat)Pb samples were measured at 60°and 120°based on the neutronics integral experimental facility at the China Institute of Atomic Energy(CIAE).The^(nat)Pb sample sizes were30 cm×30 cm×5 cm,30 cm×30 cm×10 cm,and 30 cm×30 cm×15 cm.Neutron sources were generated by the Cockcroft-Walton accelerator,producing approximately 14.5 MeV and 3.5 MeV neutrons through the T(d,n)^(4)He and D(d,n)^(3)He reactions,respectively.Leakage neutron spectra were also calculated by employing the Monte Carlo code of MCNP-4C,and the nuclear data of Pb isotopes from four libraries:CENDL-3.2,JEFF-3.3,JENDL-5,and ENDF/B-Ⅷ.0 were used individually.By comparing the simulation and experimental results,improvements and deficiencies in the evaluated nuclear data of the Pb isotopes were analyzed.Most of the calculated results were consistent with the experimental results;however,a few areas did not fit well.In the(n,el)energy range,the simulated results from CENDL-3.2 were significantly overestimated;in the(n,inl)D and the(n,inl)C energy regions,the results from CENDL-3.2 and ENDF/B-Ⅷ.0 were significantly overestimated at 120°,and the results from JENDL-5 and JEFF-3.3 are underestimated at 60°in the(n,inl)D energy region.The calculated spectra were analyzed by comparing them with the experimental spectra in terms of the neutron spectrum shape and C/E values.The results indicate that the theoretical simulations,using different data libraries,overestimated or underestimated the measured values in certain energy ranges.Secondary neutron energies and angular distributions in the data files have been presented to explain these discrepancies.展开更多
文摘目的探讨NAT10在肝癌中的临床意义与潜在作用机制。方法基于癌症基因组图谱(The Cancer Genome Atlas,TCGA)获得的50例正常和374例肿瘤样本,采用R 4.2.1处理所获数据。结果NAT10在肿瘤样本中的表达显著高于正常样本(P<0.001)。与NAT10低表达患者比较,NAT10高表达患者预后较差(P<0.001)。Logistic回归分析结果表明,NAT10高表达与肝癌患者临床预后不良因素相关,NAT10高表达患者更易于进展到晚期。基因集富集分析(gene set enrichment analysis,GSEA)结果显示,NAT10高表达样本中,异生物质代谢、凝血、脂肪酸代谢等相关基因特征均有差异富集。蛋白互作分析结果显示,NAT10可能与IGF2、SST、MUC2、CHGA、AGR2等基因存在相互作用。结论NAT10在肝癌中高表达,且表达水平与肝癌的临床特征及患者生存率存在相关性。NAT10可能是一种潜在的肝癌预后生物学标志物。
文摘目的通过检测结核病患者药物代谢酶N-乙酰基转移酶2(N-acetyltransferase 2,NAT2)与妊娠X受体基因(Pregnane X receptor,PXR)启动子区rs3814055的基因多态性,根据结果进行风险分型,并验证对发生结核药物性肝损伤的预测价值。方法将382例采用一线HREZ抗结核方案的敏感性结核患者在治疗前对NAT2及rs3814055的多态性位点进行测序分析,并进行风险分型,按结果分为高、中、低风险组,通过观察其实际发生肝损伤情况进行对比分析,以此验证风险类型与ATDILT间的相关性。结果低风险组患者185名,发生ATDILT的比例为15.7%(29/185);中风险组患者132名,发生ATDILT的比例为28.0%(37/132);高风险组患者65名,发生ATDILT的比例为32.3%(21/65)。高风险组与低风险组相比,发生肝损伤的相对危险度为1.909(95%CI为1.258~2.898);中风险组与低风险组相比,发生肝损伤的相对危险度为1.481(95%CI为1.135~1.933),且均有统计学意义(χ^(2)=8.316,P<0.01;χ^(2)=7.133,P<0.01)。高、中风险组间无显著性差异。结果显示患者性别与年龄对ATDILT发生率无显著性影响。结论基于NAT2及rs3814055多态性的联合检测的风险分型可用来预测结核病患者药物性肝损伤的发生。
基金supported by the National Natural Science Foundation of China(Nos.11775311 and U2067205)the Stable Support Basic Research Program Grant(BJ010261223282)the Research and Development Project of China National Nuclear Corporation。
文摘Lead(Pb)plays a significant role in the nuclear industry and is extensively used in radiation shielding,radiation protection,neutron moderation,radiation measurements,and various other critical functions.Consequently,the measurement and evaluation of Pb nuclear data are highly regarded in nuclear scientific research,emphasizing its crucial role in the field.Using the time-of-flight(ToF)method,the neutron leakage spectra from three^(nat)Pb samples were measured at 60°and 120°based on the neutronics integral experimental facility at the China Institute of Atomic Energy(CIAE).The^(nat)Pb sample sizes were30 cm×30 cm×5 cm,30 cm×30 cm×10 cm,and 30 cm×30 cm×15 cm.Neutron sources were generated by the Cockcroft-Walton accelerator,producing approximately 14.5 MeV and 3.5 MeV neutrons through the T(d,n)^(4)He and D(d,n)^(3)He reactions,respectively.Leakage neutron spectra were also calculated by employing the Monte Carlo code of MCNP-4C,and the nuclear data of Pb isotopes from four libraries:CENDL-3.2,JEFF-3.3,JENDL-5,and ENDF/B-Ⅷ.0 were used individually.By comparing the simulation and experimental results,improvements and deficiencies in the evaluated nuclear data of the Pb isotopes were analyzed.Most of the calculated results were consistent with the experimental results;however,a few areas did not fit well.In the(n,el)energy range,the simulated results from CENDL-3.2 were significantly overestimated;in the(n,inl)D and the(n,inl)C energy regions,the results from CENDL-3.2 and ENDF/B-Ⅷ.0 were significantly overestimated at 120°,and the results from JENDL-5 and JEFF-3.3 are underestimated at 60°in the(n,inl)D energy region.The calculated spectra were analyzed by comparing them with the experimental spectra in terms of the neutron spectrum shape and C/E values.The results indicate that the theoretical simulations,using different data libraries,overestimated or underestimated the measured values in certain energy ranges.Secondary neutron energies and angular distributions in the data files have been presented to explain these discrepancies.
