Background:Naodesheng tablets(NDST)was widely used in clinical treatment as a drug for cardiovascular diseases,but the mechanism for treating atherosclerosis was unknown.On the basis of network pharmacology and bioinf...Background:Naodesheng tablets(NDST)was widely used in clinical treatment as a drug for cardiovascular diseases,but the mechanism for treating atherosclerosis was unknown.On the basis of network pharmacology and bioinformatics,predict the relevant targets and signalling pathways for NDST in the treatment of atherosclerosis.Methods:First,the targets of NDST and the targets for treating atherosclerosis were looked for in different databases.Next,Venny 2.1.0 was used to find the genes that overlapped between NDST and targets for treating atherosclerosis.Subsequently,the herb-active ingredient-target-disease were obtained to explore the hub compound.Furthermore,the Metascape database was used for Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis.And we constructed the“active ingredient-intersection target-pathway”network by Cytoscape software to gain the hub genes and pathways.Finally,molecular docking was used to verify the affinity of hub ingredients and hub genes.Results:In the results,67 active ingredients and 322 targets of NDST were selected in ingredients-targets network.154 overlapping targets of NDST(322)and atherosclerosis(1330)were obtained.Then,the herb-active ingredient-target-disease showed that quercetin,apigenin and luteolin were the hub ingredients to treat atherosclerosis.Further,the hub genes(PTGS2,RXRA,CASP3)and pathways(lipid and atherosclerosis)were accessed in active ingredient-intersection target-pathway.Finally,the results indicated that quercetin,apigenin and luteolin were better binding the PTGS2,RXRA,CASP3,especially PTGS2 and luteolin in molecular docking.Conclusion:In conclusion,quercetin,apigenin and luteolin,as the main ingredients of NDST could play a important role in PTGS2,RXRA,and CASP3 for treating atherosclerosis via the lipid and atherosclerosis(TNF signaling pathway).展开更多
Objective： To study the effects and possible mechanisms of effective composite of Naodesheng （脑得生, NDS） on permanent cerebral ischemia-induced injury in rats. Methods： Male Sprague-Dawley rats with middle cereb...Objective： To study the effects and possible mechanisms of effective composite of Naodesheng （脑得生, NDS） on permanent cerebral ischemia-induced injury in rats. Methods： Male Sprague-Dawley rats with middle cerebral artery occlusion （MCAO） were established with the modified suture method, and they were randomly divided into the following groups： the sham-operated group, the model group, the Nimodipine group （0.012 g/kg）, the NDS group （1.075 g/kg）, the total extracts group （0.23 g/kg）, the high-dose NEC group （0.07 g/kg）, the middle-dose NEC group （0.02 g/kg）, and the low-dose NEC group （0.007 g/kg）. The aforesaid medicines were administered at the 2nd, 4th, and 24th h after focal cerebral ischemia, and the infarction size and water content in the brain were evaluated at the 26th h after MCAO. Then, after oral administration once daily for 7 successive days, the changes in the degree of neurological deficit, oxidative stress, and apoptosis were measured on the 7th day. Results： NEC could significantly reduce the infarction size after focal cerebral ischemia, and slightly relieve water content in the brain, significantly alleviate neurological function impairment, increase the levels of superoxide dismutase （SOD） and adenosine triphosphate enzyme （ATPase） activity, and decrease the content of malondialdehyde （MDA）. NEE; could also extenuate Bax and caspase-3 expression in the hippocampus tissue of the ischemic region. As compared with the three NEE; treated groups, the highdose NEC showed better efficacy. Conclusions： NEE; could significantly reduce brain injury induced by ischemia; its mechanism was closely associated with hindering oxidative stress and apoptosis. The effective compositeguided methodology is a feasible tool to improve the neuro-protective properties of the Chinese medicine prescription NDS against focal cerebral ischemia in rats.展开更多
Objective To investigate the therapeutic effect and possible mechanisms of Chinese ptent medicine Naodesheng（NDS） on repeated transient global cerebral ischemia（GCI） in mice. Methods The repeated transient GCI mic...Objective To investigate the therapeutic effect and possible mechanisms of Chinese ptent medicine Naodesheng（NDS） on repeated transient global cerebral ischemia（GCI） in mice. Methods The repeated transient GCI mice were induced by bilateral carotid arteries ligation, and were randomly divided into model group, Sham group without arteries ligation, NDS groups（1.25 and 2.5 g/kg） and positive control（vinpocetine 3.1 mg/kg, VP） group. After oral administration once daily for successive 7 d, the transient GCI was induced. The degree of neurological deficit, histological changes, and neurons loss in the hippocampus were evaluated. In order to investigate the possible mechanisms, the oxidative stress and inflammatory factor were measured after 24 h of GCI. Comparison among multiple groups was performed with one-way analysis of variance（ANOVA）. Results NDS could significantly alleviate the neurological function impairment, histological injury, and neurons loss, increase the superoxide dismutase（SOD） activity, decrease the content of malondialdehyde（MDA）, and reduce inflammatory factor in the ischemic brain tissue. Conclusion NDS could significantly reduce brain injury induced by global ischemia, and its mechanism is closely associated with anti-oxidation and anti-inflammation.展开更多
基金supported by a grant from Key Project of Education Commission of Hubei Province(D20202802).
文摘Background:Naodesheng tablets(NDST)was widely used in clinical treatment as a drug for cardiovascular diseases,but the mechanism for treating atherosclerosis was unknown.On the basis of network pharmacology and bioinformatics,predict the relevant targets and signalling pathways for NDST in the treatment of atherosclerosis.Methods:First,the targets of NDST and the targets for treating atherosclerosis were looked for in different databases.Next,Venny 2.1.0 was used to find the genes that overlapped between NDST and targets for treating atherosclerosis.Subsequently,the herb-active ingredient-target-disease were obtained to explore the hub compound.Furthermore,the Metascape database was used for Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis.And we constructed the“active ingredient-intersection target-pathway”network by Cytoscape software to gain the hub genes and pathways.Finally,molecular docking was used to verify the affinity of hub ingredients and hub genes.Results:In the results,67 active ingredients and 322 targets of NDST were selected in ingredients-targets network.154 overlapping targets of NDST(322)and atherosclerosis(1330)were obtained.Then,the herb-active ingredient-target-disease showed that quercetin,apigenin and luteolin were the hub ingredients to treat atherosclerosis.Further,the hub genes(PTGS2,RXRA,CASP3)and pathways(lipid and atherosclerosis)were accessed in active ingredient-intersection target-pathway.Finally,the results indicated that quercetin,apigenin and luteolin were better binding the PTGS2,RXRA,CASP3,especially PTGS2 and luteolin in molecular docking.Conclusion:In conclusion,quercetin,apigenin and luteolin,as the main ingredients of NDST could play a important role in PTGS2,RXRA,and CASP3 for treating atherosclerosis via the lipid and atherosclerosis(TNF signaling pathway).
基金Supported by Chinese Herbs Special Project of State Administration of Traditional Chinese Medicine(No. 02-03-ZP08)National High Technology Research and Development Program of China,the Fund of Research and Application of Screening Technology Platform of Innovative Drug (863 Program,No.2004AA2Z3782)
文摘Objective： To study the effects and possible mechanisms of effective composite of Naodesheng （脑得生, NDS） on permanent cerebral ischemia-induced injury in rats. Methods： Male Sprague-Dawley rats with middle cerebral artery occlusion （MCAO） were established with the modified suture method, and they were randomly divided into the following groups： the sham-operated group, the model group, the Nimodipine group （0.012 g/kg）, the NDS group （1.075 g/kg）, the total extracts group （0.23 g/kg）, the high-dose NEC group （0.07 g/kg）, the middle-dose NEC group （0.02 g/kg）, and the low-dose NEC group （0.007 g/kg）. The aforesaid medicines were administered at the 2nd, 4th, and 24th h after focal cerebral ischemia, and the infarction size and water content in the brain were evaluated at the 26th h after MCAO. Then, after oral administration once daily for 7 successive days, the changes in the degree of neurological deficit, oxidative stress, and apoptosis were measured on the 7th day. Results： NEC could significantly reduce the infarction size after focal cerebral ischemia, and slightly relieve water content in the brain, significantly alleviate neurological function impairment, increase the levels of superoxide dismutase （SOD） and adenosine triphosphate enzyme （ATPase） activity, and decrease the content of malondialdehyde （MDA）. NEE; could also extenuate Bax and caspase-3 expression in the hippocampus tissue of the ischemic region. As compared with the three NEE; treated groups, the highdose NEC showed better efficacy. Conclusions： NEE; could significantly reduce brain injury induced by ischemia; its mechanism was closely associated with hindering oxidative stress and apoptosis. The effective compositeguided methodology is a feasible tool to improve the neuro-protective properties of the Chinese medicine prescription NDS against focal cerebral ischemia in rats.
文摘Objective To investigate the therapeutic effect and possible mechanisms of Chinese ptent medicine Naodesheng（NDS） on repeated transient global cerebral ischemia（GCI） in mice. Methods The repeated transient GCI mice were induced by bilateral carotid arteries ligation, and were randomly divided into model group, Sham group without arteries ligation, NDS groups（1.25 and 2.5 g/kg） and positive control（vinpocetine 3.1 mg/kg, VP） group. After oral administration once daily for successive 7 d, the transient GCI was induced. The degree of neurological deficit, histological changes, and neurons loss in the hippocampus were evaluated. In order to investigate the possible mechanisms, the oxidative stress and inflammatory factor were measured after 24 h of GCI. Comparison among multiple groups was performed with one-way analysis of variance（ANOVA）. Results NDS could significantly alleviate the neurological function impairment, histological injury, and neurons loss, increase the superoxide dismutase（SOD） activity, decrease the content of malondialdehyde（MDA）, and reduce inflammatory factor in the ischemic brain tissue. Conclusion NDS could significantly reduce brain injury induced by global ischemia, and its mechanism is closely associated with anti-oxidation and anti-inflammation.
