Enzyme-powered micro/nanomotors(MNMs)(EMNMs)use natural enzymes to facilitate the decomposition of fuels,including hydrogen peroxide(H2O2),glucose,triglycerides,and urea to provide power.EMNMs can achieve self-propuls...Enzyme-powered micro/nanomotors(MNMs)(EMNMs)use natural enzymes to facilitate the decomposition of fuels,including hydrogen peroxide(H2O2),glucose,triglycerides,and urea to provide power.EMNMs can achieve self-propulsion through the in situ utilization of biofuels without additional fuels,exhibiting excellent biocompatibility and significant potential for application in the biomedical field.Compared with H_(2)O_(2),which may cause oxidative damage to the body,urea exhibits superior biosafety characteristics.Presently,urease-powered MNMs(UMNMs)have made notable progress in their applications in the biomedical field and have garnered considerable attention from researchers.In this review,we present the latest advancements in the biomedical field of UMNMs,primarily focusing on:1)diverse materials used for constructing the fundamental framework of motors;2)control of motor movement through the regulation of enzymatic reaction rates;and 3)research directions for the clinical application of motors,including in vivo imaging,biomarker detection,cancer treatment,optical therapy,overcoming biological barriers,antibacterial interventions,antithrombotic strategies,and gastric disease management.Despite showing immense potential in biomedical applications,there are still several challenges impeding its practical implementation,such as maintaining activity in the in vivo environment while accurately targeting specific sites to achieve the desired clinical therapeutic effects.展开更多
In the treatment of breast cancer,the combination of glutamine metabolism inhibition and photothermal therapy(PTT)is gaining increasing attention.This study developed a Janus nanomotor to enhance permeability in tumor...In the treatment of breast cancer,the combination of glutamine metabolism inhibition and photothermal therapy(PTT)is gaining increasing attention.This study developed a Janus nanomotor to enhance permeability in tumor tissues for nanomedicine applications by using mesoporous organic silica(PMO)anisotropic ally grown on the surface of the platinum(Pt)nanoparticles(PMO@Pt).The prepared PMO@Pt had unique Janus structure with an average size of approximately 236 nm.The loading capacity of V9302 was evaluated to be 44.37%when the mass ratio of V9302 to PMO@Pt was maintained at 2.0 and in vitro release studies demonstrated that acidic environments significantly enhanced the drug release.Then this nanomotor was loaded with perfluorohexane(PFH),a phase-change material,and the glutamine inhibitor V9302(denoted as Janus PMO@Pt@PFH@V9302,JPV).Janus PMO@Pt@PFH(JPP)nanomotors demonstrated enhanced fluorescence intensity and distribution within 3D tumor spheroids compared to Janus PMO@Pt nanomotors,attributed to the photothermal-induced phase change of PFH.The nanomotors exhibited high biocompatibility,with cell viability exceeding 98%at high concentrations.However,the incorporation of V9302 into the nanomotors(JPV)significantly reduced 4T1 cell viability under laser irradiation,indicating a cytotoxic effect resulting from the synergy between photothermal therapy and glutamine metabolism inhibition.In vivo,JPV nanomotors effectively inhibited tumor growth and induced apoptosis without causing significant systemic toxicity,showcasing their potential as a therapeutic agent for breast cancer.This integrated nanomotor offers a promising approach for enhanced ultrasound imaging and photothermal therapy in cancer treatment.展开更多
Disulfidptosis,a novel mechanism of programmed cell death through the disruption of tumor metabolic symbiosis(TMS),has showed tremendous potential in cancer therapy.However,the efficacy of disulfidptosis is limited by...Disulfidptosis,a novel mechanism of programmed cell death through the disruption of tumor metabolic symbiosis(TMS),has showed tremendous potential in cancer therapy.However,the efficacy of disulfidptosis is limited by poor permeability of drugs in solid tumors.Herein,hydrogen sulfide(H_(2)S)and nearinfrared(NIR)light-driven nanomotors(denoted as HGPP)have been constructed to efficiently penetrate tumors and induce disulfidptosis.HGPP demonstrate glutathione(GSH)-responsive release of H_(2)S,which combined with NIR light-induced photothermal effect drive HGPP movement to facilitate deep tumor penetration.The released H_(2)S induces tumor acidosis and disrupts TMS,where disulfide accumulation following cell starvation leads to disulfidptosis.In addition,HGPP induce hepatoma specific cellular uptake and catalyze the conversion of glucose and oxygen to produce hydrogen peroxide(H_(2)O_(2)),leading to glucose starvation.Overall,this study has developed a multifunctional Janus nanomotor that provides a novel strategy for disulfidptosis-based solid tumor therapy.展开更多
Micro/nanomotors have been extensively explored for efficient cancer diagnosis and therapy,as evidenced by significant breakthroughs in the design of micro/nanomotors-based intelligent and comprehensive biomedical pla...Micro/nanomotors have been extensively explored for efficient cancer diagnosis and therapy,as evidenced by significant breakthroughs in the design of micro/nanomotors-based intelligent and comprehensive biomedical platforms.Here,we demonstrate the recent advances of micro/nanomotors in the field of cancer-targeted delivery,diagnosis,and imaging-guided therapy,as well as the challenges and problems faced by micro/nanomotors in clinical applications.The outlook for the future development of micro/nanomotors toward clinical applications is also discussed.We hope to highlight these new advances in micro/nanomotors in the field of cancer diagnosis and therapy,with the ultimate goal of stimulating the successful exploration of intelligent micro/nanomotors for future clinical applications.展开更多
Due to their tiny size,autonomous motion and functionalize modifications,micro/nanomotors have shown great potential for environmental remediation,biomedicine and micro/nano-engineering.One-dimensional(1D)micro/nanomo...Due to their tiny size,autonomous motion and functionalize modifications,micro/nanomotors have shown great potential for environmental remediation,biomedicine and micro/nano-engineering.One-dimensional(1D)micro/nanomotors combine the characteristics of anisotropy and large aspect ratio of 1D materials with the advantages of functionalization and autonomous motion of micro/nanomotors for revolutionary applications.In this review,we discuss current research progress on 1D micro/nanomotors,including the fabrication methods,driving mechanisms,and recent advances in environmental remediation and biomedical applications,as well as discuss current challenges and possible solutions.With continuous attention and innovation,the advancement of 1D micro/nanomotors will pave the way for the continued development of the micro/nanomotor field.展开更多
The complex tumor microenvironment(TME)with the characteristics of severe hypoxia,enriched hydro-gen peroxide(H_(2)O_(2))and dense nature significantly restricted the therapeutic efficacy of nanomedicine in cancer tre...The complex tumor microenvironment(TME)with the characteristics of severe hypoxia,enriched hydro-gen peroxide(H_(2)O_(2))and dense nature significantly restricted the therapeutic efficacy of nanomedicine in cancer treatment.Synthetic micro/nanomotors have shown multiple versatility in modulating the abnor-mal TME and overcoming the limited penetration in solid tumor.Herein,we constructed a chemical-NIR dual-propelled nanomotor based on CuS/Pt Janus nanoparticles with IR820 encapsulation for hypoxia alle-viation,deep tumor penetration and augmented synergistic photodynamic(PDT)and photothermal ther-apy(PTT).The deposited Pt effectively catalyzed tumor endogenous H_(2)O_(2) into oxygen,which extremely relieved the tumor hypoxia state and allowed the chemical propulsion of nanomotors.Under NIR irra-diation,the Janus nanomotors exhibited more obvious movement via efficient photothermal conversion.Such autonomous motion significantly improved the tumoral accumulation of nanomotors and facilitated much deeper penetration inside tumor in vivo.In addition,enriched oxygen also promoted the genera-tion of reactive oxygen species(ROS)for augment of PDT,which achieved satisfied antitumor effect in combination with the PTT treatment.Therefore,this strategy based on CuS/Pt Janus nanomotors would provide an innovative dimension for considerable applications in effective cancer management.展开更多
We report a new facile light-induced strategy to disperse micron-sized aggregated bulk covalent organic frameworks(COFs)into isolated COFs nanoparticles.This was achieved by a series of metal-coordinated COFs,namely C...We report a new facile light-induced strategy to disperse micron-sized aggregated bulk covalent organic frameworks(COFs)into isolated COFs nanoparticles.This was achieved by a series of metal-coordinated COFs,namely COF-909-Cu,-Co or-Fe,where for the first time the diffusio-phoretic propulsion was utilized to design COF-based micro/nanomotors.The mechanism studies revealed that the metal ions decorated in the COF-909 backbone could promote the separation of electron and holes and trigger the production of sufficient ionic and reactive oxygen species under visible light irradiation.In this way,strong light-induced self-diffusiophoretic effect is achieved,resulting in good dispersion of COFs.Among them,COF-909-Fe showed the highest dispersion performance,along with a drastic decrease in particle size from 5μm to500 nm,within only 30 min light irradiation,which is inaccessible by using traditional magnetic stirring or ultrasonication methods.More importantly,benefiting from the outstanding dispersion efficiency,COF-909-Fe micro/nanomotors were demonstrated to be efficient in photocatalytic degradation of tetracycline,about 8 times faster than using traditional magnetic stirring method.This work opens up a new avenue to prepare isolated nanosized COFs in a high-fast,simple,and green manner.展开更多
The dense extracellular matrix and high interstitial pressure within tumors hinder nanoparticle penetration,reducing therapeutic efficacy.To address this,we engineered a dual-driven nanomotor based on a diselenide met...The dense extracellular matrix and high interstitial pressure within tumors hinder nanoparticle penetration,reducing therapeutic efficacy.To address this,we engineered a dual-driven nanomotor based on a diselenide metal-organic framework(MOF)using a layer-by-layer assembly process for multimodal synergistic tumor therapy.Diselenide-containing imidazole derivatives coordinated with Zn2+form the MOF,sequentially encapsulating near-infrared-Ⅱ(NIR-Ⅱ)photothermal-responsive gold nanorods(AuRods),Mn_(2)CO_(10)(MnCO),and glucose oxidase(GOD).The nanoparticle surface was functionalized with 4T1 cancer cell membranes(DSACGM NPs),guiding it to drive toward the tumor site.The photothermal effect of AuRods and CO release drives nanomotor propulsion,enhancing tumor tissue penetration.GOD catalyzes glucose(Glu)oxidation,inducing tumor starvation,while the resulting H_(2)O_(2)triggers CO release,suppressing heat shock protein(HSP)expression and enhancing mild photothermal therapy(PTT).The release of CO and the Mn^(2+)-triggered Fenton-like reaction from MnCO increased intracellular ROS levels,while diselenide depletion of glutathione(GSH)amplified chemodynamic therapy(CDT).In vitro and in vivo experiments show that DSACGM NPs induce cancer cell apoptosis under NIR-Ⅱirradiation and efficiently ablate tumors in mice at sub-hyperthermic temperatures(<45℃)with excellent biocompatibility.This study provides valuable insights into nanomedicine design and its potential in advanced tumor therapies.展开更多
Enzyme-powered micro/nanomotors(EMNMs)represent cutting-edge research taking advantage of enzymes as biocatalysts to provide a driving force for micro/nanomotors.Up to now,EMNMs have been designed to be powered by cat...Enzyme-powered micro/nanomotors(EMNMs)represent cutting-edge research taking advantage of enzymes as biocatalysts to provide a driving force for micro/nanomotors.Up to now,EMNMs have been designed to be powered by catalase,urease,lipase,collagenase,compound enzymes,etc.They not only have good biocompatibility and biosafety but also possess the unique ability to utilize physiologically relevant fuel to achieve autonomous propulsion through in vivo catalytic reactions.This innovation has opened exciting possibilities for medical applications of EMNMs.Given the fact that the human body is naturally abundant with substrates available for enzymatic reactions,EMNMs can effectively exploit the complex microenvironment associated with diseases,enabling the diagnosis and treatment of various medical conditions.In this review,we first introduce different kinds of EMNMs applied in specific environments for the diagnosis and treatment of diseases,while highlighting their advancements for revolutionizing healthcare practices.Then,we address the challenges faced in this rapidly evolving field,and at last,the potential future development directions are discussed.As the potential of EMNMs becomes increasingly evident,continued research and exploration are essential to unlock their full capabilities and to ensure their successful integration into clinical applications.展开更多
Micro/nanomotors(MNMs)have recently emerged as highly promising drug delivery vehicles,showing great potential for biomedical applications.MNMs are typically classified based on their driving mechanisms,and one notabl...Micro/nanomotors(MNMs)have recently emerged as highly promising drug delivery vehicles,showing great potential for biomedical applications.MNMs are typically classified based on their driving mechanisms,and one notable category is gas-driven MNMs,which are self-propelled at the micro/nano scale by gases generated through chemical reactions.These motors can effectively overcome various physiological barriers by utilizing unique physiological actions and driving forces in vivo,gas-driven MNMs offer significant advantages in treating diseases such as tumors and thrombosis.This review first explores the underlying mechanisms of gas-driven MNMs,then discusses their recent applications in overcoming physiological barriers.Finally,it analyses their future prospects and advantages,aiming to inspire further research and accelerate clinical translation in the biomedical field.展开更多
The realm of micro/nanomotors(MNMs)is continuously witnessing significant advancements,with multimodal propulsion emerging as a potential strategy to address the limitations of singlemode propulsion systems,such as lo...The realm of micro/nanomotors(MNMs)is continuously witnessing significant advancements,with multimodal propulsion emerging as a potential strategy to address the limitations of singlemode propulsion systems,such as low propulsion efficiency and limited versatility.The multimodal propulsion MNMs hold great promise in addressing challenges of MNM performing tasks in complex environments,offering enhanced adaptability and performance.We comprehensively review the core mechanisms of multimodal propulsion,driven by the combination of chemical,physical,and biological stimuli,and their synergistic effects in driving the movement of these MNMs.Furthermore,we delve into material design innovations in multimodal MNMs,highlighting the importance of metal-based materials,semiconductor-based materials,and polymer-based materials in enhancing their performance and responsiveness.In terms of fabrication techniques,we examine the role of template-assisted synthesis,layer-by-layer assembly,and selfassembly methods in creating complex and precise MNM structures.We specify emerging applications of multimodal MNMs,highlighting their efficacy in precise diagnosis and therapy,environmental remediation,as well as micromanipulation and assembly.Future research directions and perspectives,emphasizing the need for continuous innovation to fully harness the capabilities of these MNMs,are also elaborated.This review aims to provide a comprehensive understanding of the propulsion mechanisms,fabrication techniques,and applications of the multimodal MNMs,thereby serving as a springboard for further advancements in the field of MNMs.展开更多
Photothermal nanomotors driven by near-infrared(NIR)light emerged as a promising advancement in nanoscale propulsion systems.In this study,a novel type of nanomotor actuated by NIR light was prepared by decorating sph...Photothermal nanomotors driven by near-infrared(NIR)light emerged as a promising advancement in nanoscale propulsion systems.In this study,a novel type of nanomotor actuated by NIR light was prepared by decorating spherical TiO_(2) nanoparticles with Janus Ag-Ag_(2)S nanoparticles.The motion of these nanomotors is studied using optical microscopy with a dual light source.It is found that they can be actuated with a 700 nm driving light and traverse significant distances relative to their size.Motion analysis reveals that their maximum velocity reaches~20μm·s^(-1),or about 100 diameters per second.Statistical analysis of over 400 nanomotor trajectories shows that around 60% of them move at maximum velocities of 6 to 12μm·s^(-1).Vacuum ultraviolet velocity map imaging photoemission spectroscopy(VMI-PES)is conducted on isolated TiO_(2) and Janus Ag-Ag_(2)S nanoparticles to elucidate electronic level alignment in the hybrid particle.The findings suggest that photothermal,rather than photocatalytic,effects drive nanomotor activation under NIR light.Additionally,our calculations indicate that the difference in absorption cross-sections between Ag-Ag_(2)S and TiO_(2) components generates a temperature gradient(and consequently a pressure gradient)along the nanomotor,which in turn drives its motion.The local temperature rise near the nanomotors is a result of both photothermal effects within individual nanoparticles and thermal interactions between them.展开更多
Bionic micro/nanomotor systems,which combine biomimetic design with the motion performance,have shown great potential in many fields.However,so far,it remains a challenge to design and fabricate biomimetic micro/nanom...Bionic micro/nanomotor systems,which combine biomimetic design with the motion performance,have shown great potential in many fields.However,so far,it remains a challenge to design and fabricate biomimetic micro/nanomotors with high flexibility to perform complex tasks in complicated and changeable environments.In this work,inspired by the suckerfishes(vip)-shark(host)motion behavior,we designed and prepared a kind of intelligent two-stage micro@nanomotor with weak acid-triggered release of nanomotor.When the suckerfishes,who clinged to the surface of large fish or the bottom of boat and marched with them,reached bait-rich waters,they detached from the host to engage in foraging behavior.Inspired by the suckerfishes-shark system and the coordinated bond interaction,a large amount of Janus Au-Pt nanomotors with hydrogen peroxide(H_(2)O_(2))-driven capacity,analogous to suckerfishes,were attached onto immovable yolk-shell structured polydopamine-mesoporous silica(PDA-MS)micromotor as the host to create two-stage PDA-MS@Au-Pt micro@nanomotor.PDA-MS@Au-Pt micro@nanomotor moved directionally by self-thermophoresis under the propulsion of near infrared ray(NIR)light with low power density.When the PDA-MS@Au-Pt entered into the weak acidic environment formed by a low concentration of H_(2)O_(2),most small Au-Pt nanomotors were detached from the surface of PDA-MS due to the weak acidic sensitivity of the coordinated bond,and then performed self-diffusiophoresis in the environment containing a low concentration of H_(2)O_(2) as a chemical fuel.This bionic intelligent system,which consists of a large-sized micromotor and lots of small-sized nanomotors,should provide a new insight for active two-stage cargo delivery.展开更多
Solid tumors always exhibit local hypoxia,resulting in the high metastasis and inertness to chemotherapy.Reconstruction of hypoxic tumor microenvironment(TME)is considered a potential therapy compared to directly kill...Solid tumors always exhibit local hypoxia,resulting in the high metastasis and inertness to chemotherapy.Reconstruction of hypoxic tumor microenvironment(TME)is considered a potential therapy compared to directly killing tumor cells.However,the insufficient oxygen delivery to deep tumor and the confronting Warburg effect"compromise the efficacy of hypoxia alleviation.Herein,we construct a cascade enzyme-powered nanomotor(NM-si),which can simultaneously provide sufficient oxygen in deep tumor and inhibit the aerobic glycolysis to potentiate anti-metastasis in chemotherapy.Catalase(Cat)and glucose oxidase(GOx)are co-adsorbed on our previously reported CAuNCs@HA to form self-propelled nanomotor(NM),with hexokinase-2(HK-2)siRNA further condensed(NM-si).The persistent production of oxygen bubbles from the cascade enzymatic reaction propels NM-si to move forward autonomously and in a controllable direction along H_(2)O_(2)gradient towards deep tumor,with hypoxia successfully alleviated in the meantime.The autonomous movement also facilitates NM-si with lysosome escaping for efficient HK-2 knockdown to inhibit glycolysis.In vivo results demonstrated a promising anti-metastasis effect of commercially available albumin-bound paclitaxel(PTX@HSA)after pre-treated with NM-si for TME reconstruction.This cascade enzyme-powered nanomotor provides a potential prospect in reversing the hypoxic TME and metabolic pathway for reinforced anti-metastasis of chemotherapy.展开更多
Varieties of contrast agents have been developed for photoacoustic(PA)and ultrasound(US)imaging of cancers in vivo.However,access of traditional contrast agents into the sites of tumors has been principally through pa...Varieties of contrast agents have been developed for photoacoustic(PA)and ultrasound(US)imaging of cancers in vivo.However,access of traditional contrast agents into the sites of tumors has been principally through passive infiltration without any external force,preventing their deep penetration into the tissues of the tumors,and hindering the use of PA and US for deep tumor imaging.The concept of micro/nanomotors has been the focus of increasing attention as active theranostic agents due to their active movement in particular fluids,thereby conducting assigned tasks.Herein,US-propelled Janus mesoporous SiO_(2)partially coated gold nanorods(Au NR-mSiO_(2))were fabricated for deep tumor NIR-II PA imaging and synergistic sonodynamic-gas therapy.Following US irradiation,2,2-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride(AIPH)loaded in mSiO_(2)(Au NR-mSiO_(2)/AIPH)generated N_(2)microbubbles with high efficiency to achieve nanomotor drive.Due to the deep penetration of US,the nanomotors exhibited a capability to travel deep within sites of tumors,providing enhanced PA/US imaging inside the tumors.Furthermore,the nanomotor based cancer therapy was demonstrated through synergistic N_(2)gas and sonodynamic therapy.The US-propelled nanomotors demonstrated a novel strategy for the simultaneous PA/US dual imaging deep within tumor tissues and precise therapy of large tumors.展开更多
Overexpression of glutathione(GSH) in tumor cells greatly inhibits the therapy effect of traditional ferroptosis inducers;thus,control of the GSH level is an important way to improve the efficacy of ferroptosis.Herein...Overexpression of glutathione(GSH) in tumor cells greatly inhibits the therapy effect of traditional ferroptosis inducers;thus,control of the GSH level is an important way to improve the efficacy of ferroptosis.Herein,a kind of nanomotor based on metal organic framework material NH_(2)-MIL-101 is constructed,in which polyethylene glycol(PEG) and glutathione hydrolase γ-glutamyltransferase(GGT) are asymmetrically modified to obtain mPEG@MIL-101@GGT nanomotors(PMG NMs).The nanomotor proposed in this article can be induced by overexpressing GSH in tumors to form chemotactic effects through the specific affinity between enzymes and substrates.Results indicate that the tail structure provided by PEG and the affinity between GGT and GSH can enable the stable chemotaxis behavior of nanomotors in a complex environment,thus enriching and penetrating deeply at the tumor site.In addition,after loading the ferroptosis inducer Erastin,the system shows a highly effective induction effect of tumor ferroptosis.Erastin in the system can effectively inhibit the synthesis of GSH,and PMG NMS can react with GSH through Fe^(3+)and GGT to promote GSH depletion.The produced Fe^(2+)can generate excessive reactive oxygen species through Fenton reaction,which further promotes the death of tumor cells.Meantime,the chemotaxis behavior of the nanomotors based on the endogenous biochemical reaction of GGT-catalyzed GSH hydrolysis can endow nanomotors with the enhanced delivery and penetration ability in tumors,thus collaboratively enhancing the ferroptosis effect.This strategy designed according to the physiological characteristics of tumors has good biosafety and treatment effect,providing new perspectives for micro/nanomotor and tumor treatment.展开更多
Blood lead(Pb(II))removal is very important but challenging.The main difficulty of blood Pb(II)removal currently lies in the fact that blood Pb(II)is mainly complexed with hemoglobin(Hb)inside the red blood cells(RBCs...Blood lead(Pb(II))removal is very important but challenging.The main difficulty of blood Pb(II)removal currently lies in the fact that blood Pb(II)is mainly complexed with hemoglobin(Hb)inside the red blood cells(RBCs).Traditional blood Pb(II)removers are mostly passive particles that do not have the motion ability,thus the efficiency of the contact between the adsorbent and the Pb(II)-contaminated Hb is relatively low.Herein,a kind of magnetic nanomotor adsorbent with movement ability under alternating magnetic field based on Fe3O4 nanoparticle modified with meso-2,3-dimercaptosuccinic acid(DMSA)was prepared and a blood Pb(II)removal strategy was further proposed.During the removal process,the nanomotor adsorbent can enter the RBCs,then the contact probability between the nanomotor adsorbent and the Pb(II)-contaminated Hb can be increased by the active movement of nanomotor.Through the strong coordination of functional groups in DMSA,the nanomotor adsorbent can adsorb Pb(II),and finally be separated from blood by permanent magnetic field.The in vivo extracorporeal blood circulation experiment verifies the ability of the adsorbent to remove blood Pb(II)in pig models,which may provide innovative ideas for blood heavy metal removal in the future.展开更多
Most of the current nanomedicine-based treatments for critical limb ischemia(CLI)only aim at promoting angiogenesis,ignoring the negative influence on the therapeutic effects caused by the complex pathological micro-e...Most of the current nanomedicine-based treatments for critical limb ischemia(CLI)only aim at promoting angiogenesis,ignoring the negative influence on the therapeutic effects caused by the complex pathological micro-environment of ischemic tissue.Herein,near-infrared(NIR)light-driven metal ion(Cu^(2+))-loaded polydopamine(PDA)nanomotors(JMPN@Cu^(2+))is designed and prepared.Due to the good antioxidant and anti-inflammatory activities of PDA,JMPN@Cu^(2+)exhibits excellent biocompatibility and significantly improves the ischemic micro-environment.Additionally,based on superior photothermal conversion effect and jellyfish-like structure,the nanomotors are quickly propelled under NIR laser with low energy intensity to acquire the ability of movement and facilitate intracellular uptake of JMPN@Cu^(2+)by endothelial cells,resulting in the enhanced pro-angiogenic effect of Cu^(2+).Moreover,in vivo experimental findings show that JMPN@Cu^(2+)combined with NIR irradiation can successfully accelerate blood flow recovery and improve muscle repair.Taking these results together,this kind of nanomotor can promote angiogenesis along with ischemic micro-environment amelioration,holding great potential applications for the treatment of limb ischemia.展开更多
Catalytic nanomotors are nano-to-micrometer-sized actuators that carry an on-board catalyst and convert local chemical fuel in solution into mechanical work. The location of this catalyst as well as the geometry of th...Catalytic nanomotors are nano-to-micrometer-sized actuators that carry an on-board catalyst and convert local chemical fuel in solution into mechanical work. The location of this catalyst as well as the geometry of the structure dictate the swimming behaviors exhibited. The nanomotors can occur naturally in organic molecules, combine natural and artificial parts to form hybrid nanomotors or be purely artificial. Fabrication techniques consist of template directed electroplating, lithography, physical vapor deposition, and other advanced growth methods. Various physical and chemical propulsion mechanisms have been proposed to explain the motion behaviors including diffusiophoresis, bubble propulsion, interracial tension gradients, and self-electrophoresis. The control and manipulation based upon external fields, catalytic alloys, and motion control through thermal modulation are discussed as well. Catalytic nanomotors represent an exciting technological challenge with the end goal being practical functional nanomachines that can perform a variety of tasks at the nanoscale.展开更多
Micro/nanoscale motors(MNMs)have been regarded as promising tools in the field of engineered regeneration due to unique property of autonomous motion.Herein,a review on the advancements of MNMs in the area of engineer...Micro/nanoscale motors(MNMs)have been regarded as promising tools in the field of engineered regeneration due to unique property of autonomous motion.Herein,a review on the advancements of MNMs in the area of engineered regeneration is presented,covering aspects from their propulsion mechanisms to their frontiers in engineered regeneration,listing the revolutionary applications in biosensing,medical imaging,drug delivery and tissue engineering.Finally,challenges and future directions of MNMs are finally discussed on the basis of the achievements.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.:82372102).
文摘Enzyme-powered micro/nanomotors(MNMs)(EMNMs)use natural enzymes to facilitate the decomposition of fuels,including hydrogen peroxide(H2O2),glucose,triglycerides,and urea to provide power.EMNMs can achieve self-propulsion through the in situ utilization of biofuels without additional fuels,exhibiting excellent biocompatibility and significant potential for application in the biomedical field.Compared with H_(2)O_(2),which may cause oxidative damage to the body,urea exhibits superior biosafety characteristics.Presently,urease-powered MNMs(UMNMs)have made notable progress in their applications in the biomedical field and have garnered considerable attention from researchers.In this review,we present the latest advancements in the biomedical field of UMNMs,primarily focusing on:1)diverse materials used for constructing the fundamental framework of motors;2)control of motor movement through the regulation of enzymatic reaction rates;and 3)research directions for the clinical application of motors,including in vivo imaging,biomarker detection,cancer treatment,optical therapy,overcoming biological barriers,antibacterial interventions,antithrombotic strategies,and gastric disease management.Despite showing immense potential in biomedical applications,there are still several challenges impeding its practical implementation,such as maintaining activity in the in vivo environment while accurately targeting specific sites to achieve the desired clinical therapeutic effects.
基金financially supported by the Major Basic Research Fund of Jiangsu Province Hospital(No.TS202401)Jiangsu Province Hospital High-level Talent Cultivation Program(PhaseⅠ)(No.CZ0121002010039)
文摘In the treatment of breast cancer,the combination of glutamine metabolism inhibition and photothermal therapy(PTT)is gaining increasing attention.This study developed a Janus nanomotor to enhance permeability in tumor tissues for nanomedicine applications by using mesoporous organic silica(PMO)anisotropic ally grown on the surface of the platinum(Pt)nanoparticles(PMO@Pt).The prepared PMO@Pt had unique Janus structure with an average size of approximately 236 nm.The loading capacity of V9302 was evaluated to be 44.37%when the mass ratio of V9302 to PMO@Pt was maintained at 2.0 and in vitro release studies demonstrated that acidic environments significantly enhanced the drug release.Then this nanomotor was loaded with perfluorohexane(PFH),a phase-change material,and the glutamine inhibitor V9302(denoted as Janus PMO@Pt@PFH@V9302,JPV).Janus PMO@Pt@PFH(JPP)nanomotors demonstrated enhanced fluorescence intensity and distribution within 3D tumor spheroids compared to Janus PMO@Pt nanomotors,attributed to the photothermal-induced phase change of PFH.The nanomotors exhibited high biocompatibility,with cell viability exceeding 98%at high concentrations.However,the incorporation of V9302 into the nanomotors(JPV)significantly reduced 4T1 cell viability under laser irradiation,indicating a cytotoxic effect resulting from the synergy between photothermal therapy and glutamine metabolism inhibition.In vivo,JPV nanomotors effectively inhibited tumor growth and induced apoptosis without causing significant systemic toxicity,showcasing their potential as a therapeutic agent for breast cancer.This integrated nanomotor offers a promising approach for enhanced ultrasound imaging and photothermal therapy in cancer treatment.
基金supported by the National Natural Science Foundation of China(No.22171230)the Project of Science and Technology of Social Development in Shaanxi Province(Nos.2024SF-YBXM-294,2023-YBSF-151)。
文摘Disulfidptosis,a novel mechanism of programmed cell death through the disruption of tumor metabolic symbiosis(TMS),has showed tremendous potential in cancer therapy.However,the efficacy of disulfidptosis is limited by poor permeability of drugs in solid tumors.Herein,hydrogen sulfide(H_(2)S)and nearinfrared(NIR)light-driven nanomotors(denoted as HGPP)have been constructed to efficiently penetrate tumors and induce disulfidptosis.HGPP demonstrate glutathione(GSH)-responsive release of H_(2)S,which combined with NIR light-induced photothermal effect drive HGPP movement to facilitate deep tumor penetration.The released H_(2)S induces tumor acidosis and disrupts TMS,where disulfide accumulation following cell starvation leads to disulfidptosis.In addition,HGPP induce hepatoma specific cellular uptake and catalyze the conversion of glucose and oxygen to produce hydrogen peroxide(H_(2)O_(2)),leading to glucose starvation.Overall,this study has developed a multifunctional Janus nanomotor that provides a novel strategy for disulfidptosis-based solid tumor therapy.
基金the Natural Science Foundation of China (Nos. 21805096 and 21671071)Natural Science Foundation of Guangdong Province (Nos. 2018A030313358 and 2017A030310432)+3 种基金Characteristic Innovation Projects of Guangdong Ordinary University (No. 2018KTSCX045)Applied Science and Technology Planning Project of Guangdong Province (Nos. 2015B010135009 and 2017B090917002)Innovation team project of Guangdong Ordinary University (No. 2015KCXTD005)the great scientific research project of Guangdong Ordinary University (No. 2016KZDXM023)
文摘Micro/nanomotors have been extensively explored for efficient cancer diagnosis and therapy,as evidenced by significant breakthroughs in the design of micro/nanomotors-based intelligent and comprehensive biomedical platforms.Here,we demonstrate the recent advances of micro/nanomotors in the field of cancer-targeted delivery,diagnosis,and imaging-guided therapy,as well as the challenges and problems faced by micro/nanomotors in clinical applications.The outlook for the future development of micro/nanomotors toward clinical applications is also discussed.We hope to highlight these new advances in micro/nanomotors in the field of cancer diagnosis and therapy,with the ultimate goal of stimulating the successful exploration of intelligent micro/nanomotors for future clinical applications.
基金supported by General Project of Natural Science Foundation of Guangdong Province(2022A1515010715)Guangzhou Basic and Applied Basic Research Project(202102020638)+4 种基金Science and Technology Planning Project of Guangdong Province(2017B090917002,2019B1515120027 and 2019A050510038)Research and development plan projects in key areas of Guangdong Province(2020B0101030005)supported by Grant PID2020-118154GB-I00 funded by MCIN/AEI/https://doi.org/10.13039/501100011033,Grant TED2021-132720B-I00,funded by MCIN/AEI/https://doi.org/10.13039/501100011033the European Union“NextGenerationEU”/PRTR(B.J.S)the Community of Madrid[grant number CM/JIN/2021-012(B.J.S)]。
文摘Due to their tiny size,autonomous motion and functionalize modifications,micro/nanomotors have shown great potential for environmental remediation,biomedicine and micro/nano-engineering.One-dimensional(1D)micro/nanomotors combine the characteristics of anisotropy and large aspect ratio of 1D materials with the advantages of functionalization and autonomous motion of micro/nanomotors for revolutionary applications.In this review,we discuss current research progress on 1D micro/nanomotors,including the fabrication methods,driving mechanisms,and recent advances in environmental remediation and biomedical applications,as well as discuss current challenges and possible solutions.With continuous attention and innovation,the advancement of 1D micro/nanomotors will pave the way for the continued development of the micro/nanomotor field.
基金financially supported by the National Natural Science Foundation of China(Nos.81901798,21905303,82172005)the Promotion Fund for Youth Talent of Jiangsu Association for Science and Technology(No.TJ-2021-069)+5 种基金the Startup Fund for Youth Talent in Xuzhou Medical University(No.D2019022)the Open Fund of Xuzhou Medical University(No.XYKF202102)the Fundamental Research Funds for the Central Universities(Nos.2020ZDPY0213,2022YCPY0205)the Natural Science Foundation of Jiangsu Province(Nos.BK20220161,BK20220663)the Jiangsu High-level Innovation and entrepreneurship Talent Project(No.RC5042001)the Jiangsu Province 6th“333 Project”of Cultivation of High-level Talents.
文摘The complex tumor microenvironment(TME)with the characteristics of severe hypoxia,enriched hydro-gen peroxide(H_(2)O_(2))and dense nature significantly restricted the therapeutic efficacy of nanomedicine in cancer treatment.Synthetic micro/nanomotors have shown multiple versatility in modulating the abnor-mal TME and overcoming the limited penetration in solid tumor.Herein,we constructed a chemical-NIR dual-propelled nanomotor based on CuS/Pt Janus nanoparticles with IR820 encapsulation for hypoxia alle-viation,deep tumor penetration and augmented synergistic photodynamic(PDT)and photothermal ther-apy(PTT).The deposited Pt effectively catalyzed tumor endogenous H_(2)O_(2) into oxygen,which extremely relieved the tumor hypoxia state and allowed the chemical propulsion of nanomotors.Under NIR irra-diation,the Janus nanomotors exhibited more obvious movement via efficient photothermal conversion.Such autonomous motion significantly improved the tumoral accumulation of nanomotors and facilitated much deeper penetration inside tumor in vivo.In addition,enriched oxygen also promoted the genera-tion of reactive oxygen species(ROS)for augment of PDT,which achieved satisfied antitumor effect in combination with the PTT treatment.Therefore,this strategy based on CuS/Pt Janus nanomotors would provide an innovative dimension for considerable applications in effective cancer management.
基金supported by Huazhong University of Science and Technology(No.2021XXJS036,3004013134)National Natural Science Foundation of China(No.51903099,82002879,22102059)+2 种基金the Innovation and Talent Recruitment Base of New Energy Chemistry and Device(No.B21003)China Postdoctoral Science Foundation(2021M692475,2021T140524,XJ2021037)support from the 100 Talents Program of the Hubei Provincial Government。
文摘We report a new facile light-induced strategy to disperse micron-sized aggregated bulk covalent organic frameworks(COFs)into isolated COFs nanoparticles.This was achieved by a series of metal-coordinated COFs,namely COF-909-Cu,-Co or-Fe,where for the first time the diffusio-phoretic propulsion was utilized to design COF-based micro/nanomotors.The mechanism studies revealed that the metal ions decorated in the COF-909 backbone could promote the separation of electron and holes and trigger the production of sufficient ionic and reactive oxygen species under visible light irradiation.In this way,strong light-induced self-diffusiophoretic effect is achieved,resulting in good dispersion of COFs.Among them,COF-909-Fe showed the highest dispersion performance,along with a drastic decrease in particle size from 5μm to500 nm,within only 30 min light irradiation,which is inaccessible by using traditional magnetic stirring or ultrasonication methods.More importantly,benefiting from the outstanding dispersion efficiency,COF-909-Fe micro/nanomotors were demonstrated to be efficient in photocatalytic degradation of tetracycline,about 8 times faster than using traditional magnetic stirring method.This work opens up a new avenue to prepare isolated nanosized COFs in a high-fast,simple,and green manner.
基金supported by the National Key R&D Program of China(2020YFA0908500)the National Natural Science Foundation of China(22161142015,22201058,and 22275046)+1 种基金the Interdisciplinary Research Project of Hangzhou Normal University(2024JCXK01)the Hangzhou Leading Innovation and Entrepreneurship Team Project of Hangzhou Science and Technology Bureau(TD2022001)。
文摘The dense extracellular matrix and high interstitial pressure within tumors hinder nanoparticle penetration,reducing therapeutic efficacy.To address this,we engineered a dual-driven nanomotor based on a diselenide metal-organic framework(MOF)using a layer-by-layer assembly process for multimodal synergistic tumor therapy.Diselenide-containing imidazole derivatives coordinated with Zn2+form the MOF,sequentially encapsulating near-infrared-Ⅱ(NIR-Ⅱ)photothermal-responsive gold nanorods(AuRods),Mn_(2)CO_(10)(MnCO),and glucose oxidase(GOD).The nanoparticle surface was functionalized with 4T1 cancer cell membranes(DSACGM NPs),guiding it to drive toward the tumor site.The photothermal effect of AuRods and CO release drives nanomotor propulsion,enhancing tumor tissue penetration.GOD catalyzes glucose(Glu)oxidation,inducing tumor starvation,while the resulting H_(2)O_(2)triggers CO release,suppressing heat shock protein(HSP)expression and enhancing mild photothermal therapy(PTT).The release of CO and the Mn^(2+)-triggered Fenton-like reaction from MnCO increased intracellular ROS levels,while diselenide depletion of glutathione(GSH)amplified chemodynamic therapy(CDT).In vitro and in vivo experiments show that DSACGM NPs induce cancer cell apoptosis under NIR-Ⅱirradiation and efficiently ablate tumors in mice at sub-hyperthermic temperatures(<45℃)with excellent biocompatibility.This study provides valuable insights into nanomedicine design and its potential in advanced tumor therapies.
基金support by the Young Taishan Scholars Program of Shandong Province(Grant No.tsqn202306272)National Key Research and Development Project of China(Grant No.2023YFFO715101)+5 种基金National Natural Science Foundation of China(Grant No.22307050)Natural Science Foundation of Shandong Province(Grant No.ZR2023QB292,ZR2024YQ068,2024HWYQ-062)the Leading Project of Science and Technology of Yantai Development Zone(Grant No.2021RC016)CFG and RLR acknowledge funding from Fundaçao para a Ciencia e Tecnologia(FCT)Grants 10.54499/2022.05711.CEECIND/CP1718/CT0012(CFG)and 10.54499/2022.05737.PTDC(CFG,RLR)as well as TERM RES Hub–Scientific Infrastructure for Tissue Engineering and Regenerative Medicine,reference PINFRA/22190/2016(Norte-01-0145-FEDER-022190).
文摘Enzyme-powered micro/nanomotors(EMNMs)represent cutting-edge research taking advantage of enzymes as biocatalysts to provide a driving force for micro/nanomotors.Up to now,EMNMs have been designed to be powered by catalase,urease,lipase,collagenase,compound enzymes,etc.They not only have good biocompatibility and biosafety but also possess the unique ability to utilize physiologically relevant fuel to achieve autonomous propulsion through in vivo catalytic reactions.This innovation has opened exciting possibilities for medical applications of EMNMs.Given the fact that the human body is naturally abundant with substrates available for enzymatic reactions,EMNMs can effectively exploit the complex microenvironment associated with diseases,enabling the diagnosis and treatment of various medical conditions.In this review,we first introduce different kinds of EMNMs applied in specific environments for the diagnosis and treatment of diseases,while highlighting their advancements for revolutionizing healthcare practices.Then,we address the challenges faced in this rapidly evolving field,and at last,the potential future development directions are discussed.As the potential of EMNMs becomes increasingly evident,continued research and exploration are essential to unlock their full capabilities and to ensure their successful integration into clinical applications.
基金supported by the National Natural Science Foundation of China(Nos.82222067,and 82102936)Outstanding Youth Foundation of Henan Province Henan(No.222300420020)+3 种基金China Postdoctoral Science Foundation(No.2023M743232)the Postdoctoral Fellowship Program of CPSF under Grant Number(No.GZB20230676)Scientific and Technological Innovation Talent in Central Plains,Key Projects of Advantageous disciplines in Henan Province(No.222301420011)Scientific and Technological Project of Henan Province(No.242102310450).
文摘Micro/nanomotors(MNMs)have recently emerged as highly promising drug delivery vehicles,showing great potential for biomedical applications.MNMs are typically classified based on their driving mechanisms,and one notable category is gas-driven MNMs,which are self-propelled at the micro/nano scale by gases generated through chemical reactions.These motors can effectively overcome various physiological barriers by utilizing unique physiological actions and driving forces in vivo,gas-driven MNMs offer significant advantages in treating diseases such as tumors and thrombosis.This review first explores the underlying mechanisms of gas-driven MNMs,then discusses their recent applications in overcoming physiological barriers.Finally,it analyses their future prospects and advantages,aiming to inspire further research and accelerate clinical translation in the biomedical field.
基金supported in part by the Hong Kong Research Grants Council(RGC)Research Impact Fund(RIF)with project No.R4015-21the Health@InnoHK program of the Innovation and Technology Commission under the Hong Kong SAR government,Hong Kong,China.
文摘The realm of micro/nanomotors(MNMs)is continuously witnessing significant advancements,with multimodal propulsion emerging as a potential strategy to address the limitations of singlemode propulsion systems,such as low propulsion efficiency and limited versatility.The multimodal propulsion MNMs hold great promise in addressing challenges of MNM performing tasks in complex environments,offering enhanced adaptability and performance.We comprehensively review the core mechanisms of multimodal propulsion,driven by the combination of chemical,physical,and biological stimuli,and their synergistic effects in driving the movement of these MNMs.Furthermore,we delve into material design innovations in multimodal MNMs,highlighting the importance of metal-based materials,semiconductor-based materials,and polymer-based materials in enhancing their performance and responsiveness.In terms of fabrication techniques,we examine the role of template-assisted synthesis,layer-by-layer assembly,and selfassembly methods in creating complex and precise MNM structures.We specify emerging applications of multimodal MNMs,highlighting their efficacy in precise diagnosis and therapy,environmental remediation,as well as micromanipulation and assembly.Future research directions and perspectives,emphasizing the need for continuous innovation to fully harness the capabilities of these MNMs,are also elaborated.This review aims to provide a comprehensive understanding of the propulsion mechanisms,fabrication techniques,and applications of the multimodal MNMs,thereby serving as a springboard for further advancements in the field of MNMs.
基金funded by the Ministry of Science,Technological development and Innovation of the Republic of Serbia under Grant Agreements No 451-03-66/2024-03/200017 and 451-03-66/2024-03/200162.
文摘Photothermal nanomotors driven by near-infrared(NIR)light emerged as a promising advancement in nanoscale propulsion systems.In this study,a novel type of nanomotor actuated by NIR light was prepared by decorating spherical TiO_(2) nanoparticles with Janus Ag-Ag_(2)S nanoparticles.The motion of these nanomotors is studied using optical microscopy with a dual light source.It is found that they can be actuated with a 700 nm driving light and traverse significant distances relative to their size.Motion analysis reveals that their maximum velocity reaches~20μm·s^(-1),or about 100 diameters per second.Statistical analysis of over 400 nanomotor trajectories shows that around 60% of them move at maximum velocities of 6 to 12μm·s^(-1).Vacuum ultraviolet velocity map imaging photoemission spectroscopy(VMI-PES)is conducted on isolated TiO_(2) and Janus Ag-Ag_(2)S nanoparticles to elucidate electronic level alignment in the hybrid particle.The findings suggest that photothermal,rather than photocatalytic,effects drive nanomotor activation under NIR light.Additionally,our calculations indicate that the difference in absorption cross-sections between Ag-Ag_(2)S and TiO_(2) components generates a temperature gradient(and consequently a pressure gradient)along the nanomotor,which in turn drives its motion.The local temperature rise near the nanomotors is a result of both photothermal effects within individual nanoparticles and thermal interactions between them.
基金supported by Fundamental Research Funds for the Central Universities(No.FRF-BR-23-02B)China Postdoctoral Science Foundation(No.2023M731408)+2 种基金Jiangsu Funding Program for Excellent Postdoctoral Talent(No.2023ZB640)Jiangsu Province Capability Improvement Project through Science,technology and Education(Jiangsu Provincial Medical Key Discipline,ZDXK202222)Natural Science Foundation of Jiangsu Province(No.BK20230731).
文摘Bionic micro/nanomotor systems,which combine biomimetic design with the motion performance,have shown great potential in many fields.However,so far,it remains a challenge to design and fabricate biomimetic micro/nanomotors with high flexibility to perform complex tasks in complicated and changeable environments.In this work,inspired by the suckerfishes(vip)-shark(host)motion behavior,we designed and prepared a kind of intelligent two-stage micro@nanomotor with weak acid-triggered release of nanomotor.When the suckerfishes,who clinged to the surface of large fish or the bottom of boat and marched with them,reached bait-rich waters,they detached from the host to engage in foraging behavior.Inspired by the suckerfishes-shark system and the coordinated bond interaction,a large amount of Janus Au-Pt nanomotors with hydrogen peroxide(H_(2)O_(2))-driven capacity,analogous to suckerfishes,were attached onto immovable yolk-shell structured polydopamine-mesoporous silica(PDA-MS)micromotor as the host to create two-stage PDA-MS@Au-Pt micro@nanomotor.PDA-MS@Au-Pt micro@nanomotor moved directionally by self-thermophoresis under the propulsion of near infrared ray(NIR)light with low power density.When the PDA-MS@Au-Pt entered into the weak acidic environment formed by a low concentration of H_(2)O_(2),most small Au-Pt nanomotors were detached from the surface of PDA-MS due to the weak acidic sensitivity of the coordinated bond,and then performed self-diffusiophoresis in the environment containing a low concentration of H_(2)O_(2) as a chemical fuel.This bionic intelligent system,which consists of a large-sized micromotor and lots of small-sized nanomotors,should provide a new insight for active two-stage cargo delivery.
基金supported by National Natural Science Foundation of China(No.81961138009)the Fundamental Research Funds for the Central Universities(Nos.SCU2017A001,2018SCUH0024,China)+1 种基金111 Project(No.B18035,China)the Key Research and Development Program of Science and Technology Department of Sichuan Province(No.2020YFS0570,China)
文摘Solid tumors always exhibit local hypoxia,resulting in the high metastasis and inertness to chemotherapy.Reconstruction of hypoxic tumor microenvironment(TME)is considered a potential therapy compared to directly killing tumor cells.However,the insufficient oxygen delivery to deep tumor and the confronting Warburg effect"compromise the efficacy of hypoxia alleviation.Herein,we construct a cascade enzyme-powered nanomotor(NM-si),which can simultaneously provide sufficient oxygen in deep tumor and inhibit the aerobic glycolysis to potentiate anti-metastasis in chemotherapy.Catalase(Cat)and glucose oxidase(GOx)are co-adsorbed on our previously reported CAuNCs@HA to form self-propelled nanomotor(NM),with hexokinase-2(HK-2)siRNA further condensed(NM-si).The persistent production of oxygen bubbles from the cascade enzymatic reaction propels NM-si to move forward autonomously and in a controllable direction along H_(2)O_(2)gradient towards deep tumor,with hypoxia successfully alleviated in the meantime.The autonomous movement also facilitates NM-si with lysosome escaping for efficient HK-2 knockdown to inhibit glycolysis.In vivo results demonstrated a promising anti-metastasis effect of commercially available albumin-bound paclitaxel(PTX@HSA)after pre-treated with NM-si for TME reconstruction.This cascade enzyme-powered nanomotor provides a potential prospect in reversing the hypoxic TME and metabolic pathway for reinforced anti-metastasis of chemotherapy.
基金supported by the National Natural Science Foundation of China(21874024,22027805,21804068)the National Key R&D Program of China(2020YFA0210800)+1 种基金the joint re-search projects of Health and Education Commission of Fujian Province(2019-WJ-20)the Natural Science Foundation of Fujian Province(2020J02012)。
文摘Varieties of contrast agents have been developed for photoacoustic(PA)and ultrasound(US)imaging of cancers in vivo.However,access of traditional contrast agents into the sites of tumors has been principally through passive infiltration without any external force,preventing their deep penetration into the tissues of the tumors,and hindering the use of PA and US for deep tumor imaging.The concept of micro/nanomotors has been the focus of increasing attention as active theranostic agents due to their active movement in particular fluids,thereby conducting assigned tasks.Herein,US-propelled Janus mesoporous SiO_(2)partially coated gold nanorods(Au NR-mSiO_(2))were fabricated for deep tumor NIR-II PA imaging and synergistic sonodynamic-gas therapy.Following US irradiation,2,2-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride(AIPH)loaded in mSiO_(2)(Au NR-mSiO_(2)/AIPH)generated N_(2)microbubbles with high efficiency to achieve nanomotor drive.Due to the deep penetration of US,the nanomotors exhibited a capability to travel deep within sites of tumors,providing enhanced PA/US imaging inside the tumors.Furthermore,the nanomotor based cancer therapy was demonstrated through synergistic N_(2)gas and sonodynamic therapy.The US-propelled nanomotors demonstrated a novel strategy for the simultaneous PA/US dual imaging deep within tumor tissues and precise therapy of large tumors.
基金supported by the National Natural Science Foundation of China (22175096)the Social Development Project of Jiangsu Natural Science Foundation (BE2019744)+1 种基金the Collaborative Innovation Center of Biomedical Functional Materialsthe Priority Academic Program Development of Jiangsu Higher Education Institution。
文摘Overexpression of glutathione(GSH) in tumor cells greatly inhibits the therapy effect of traditional ferroptosis inducers;thus,control of the GSH level is an important way to improve the efficacy of ferroptosis.Herein,a kind of nanomotor based on metal organic framework material NH_(2)-MIL-101 is constructed,in which polyethylene glycol(PEG) and glutathione hydrolase γ-glutamyltransferase(GGT) are asymmetrically modified to obtain mPEG@MIL-101@GGT nanomotors(PMG NMs).The nanomotor proposed in this article can be induced by overexpressing GSH in tumors to form chemotactic effects through the specific affinity between enzymes and substrates.Results indicate that the tail structure provided by PEG and the affinity between GGT and GSH can enable the stable chemotaxis behavior of nanomotors in a complex environment,thus enriching and penetrating deeply at the tumor site.In addition,after loading the ferroptosis inducer Erastin,the system shows a highly effective induction effect of tumor ferroptosis.Erastin in the system can effectively inhibit the synthesis of GSH,and PMG NMS can react with GSH through Fe^(3+)and GGT to promote GSH depletion.The produced Fe^(2+)can generate excessive reactive oxygen species through Fenton reaction,which further promotes the death of tumor cells.Meantime,the chemotaxis behavior of the nanomotors based on the endogenous biochemical reaction of GGT-catalyzed GSH hydrolysis can endow nanomotors with the enhanced delivery and penetration ability in tumors,thus collaboratively enhancing the ferroptosis effect.This strategy designed according to the physiological characteristics of tumors has good biosafety and treatment effect,providing new perspectives for micro/nanomotor and tumor treatment.
基金Social development project of Jiangsu Natural Science Foundation(No:BE2019744)Jiangsu Collaborative Innovation Center of Biomedical Functional Materials,National Natural Science Foundation of China(51641104,21603105)+1 种基金Natural Science Foundation of Jiangsu Province(BK20171115)the Priority Academic Program Development of Jiangsu Higher Education Institution.
文摘Blood lead(Pb(II))removal is very important but challenging.The main difficulty of blood Pb(II)removal currently lies in the fact that blood Pb(II)is mainly complexed with hemoglobin(Hb)inside the red blood cells(RBCs).Traditional blood Pb(II)removers are mostly passive particles that do not have the motion ability,thus the efficiency of the contact between the adsorbent and the Pb(II)-contaminated Hb is relatively low.Herein,a kind of magnetic nanomotor adsorbent with movement ability under alternating magnetic field based on Fe3O4 nanoparticle modified with meso-2,3-dimercaptosuccinic acid(DMSA)was prepared and a blood Pb(II)removal strategy was further proposed.During the removal process,the nanomotor adsorbent can enter the RBCs,then the contact probability between the nanomotor adsorbent and the Pb(II)-contaminated Hb can be increased by the active movement of nanomotor.Through the strong coordination of functional groups in DMSA,the nanomotor adsorbent can adsorb Pb(II),and finally be separated from blood by permanent magnetic field.The in vivo extracorporeal blood circulation experiment verifies the ability of the adsorbent to remove blood Pb(II)in pig models,which may provide innovative ideas for blood heavy metal removal in the future.
基金supported by the National Natural Science Foundation of China(No.82170515)Open Research Fund of Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy(No.XZSYSKF2021038)Jiangsu Funding Program for Excellent Postdoctoral Talent,and Changzhou Municipal Health Commission Science and Technology Project(No.ZD202126).
文摘Most of the current nanomedicine-based treatments for critical limb ischemia(CLI)only aim at promoting angiogenesis,ignoring the negative influence on the therapeutic effects caused by the complex pathological micro-environment of ischemic tissue.Herein,near-infrared(NIR)light-driven metal ion(Cu^(2+))-loaded polydopamine(PDA)nanomotors(JMPN@Cu^(2+))is designed and prepared.Due to the good antioxidant and anti-inflammatory activities of PDA,JMPN@Cu^(2+)exhibits excellent biocompatibility and significantly improves the ischemic micro-environment.Additionally,based on superior photothermal conversion effect and jellyfish-like structure,the nanomotors are quickly propelled under NIR laser with low energy intensity to acquire the ability of movement and facilitate intracellular uptake of JMPN@Cu^(2+)by endothelial cells,resulting in the enhanced pro-angiogenic effect of Cu^(2+).Moreover,in vivo experimental findings show that JMPN@Cu^(2+)combined with NIR irradiation can successfully accelerate blood flow recovery and improve muscle repair.Taking these results together,this kind of nanomotor can promote angiogenesis along with ischemic micro-environment amelioration,holding great potential applications for the treatment of limb ischemia.
基金Acknowledgements We acknowledge the financial support from the National Science Foundation under Contract No. CMMI-0726770 and ECCS-0901141.
文摘Catalytic nanomotors are nano-to-micrometer-sized actuators that carry an on-board catalyst and convert local chemical fuel in solution into mechanical work. The location of this catalyst as well as the geometry of the structure dictate the swimming behaviors exhibited. The nanomotors can occur naturally in organic molecules, combine natural and artificial parts to form hybrid nanomotors or be purely artificial. Fabrication techniques consist of template directed electroplating, lithography, physical vapor deposition, and other advanced growth methods. Various physical and chemical propulsion mechanisms have been proposed to explain the motion behaviors including diffusiophoresis, bubble propulsion, interracial tension gradients, and self-electrophoresis. The control and manipulation based upon external fields, catalytic alloys, and motion control through thermal modulation are discussed as well. Catalytic nanomotors represent an exciting technological challenge with the end goal being practical functional nanomachines that can perform a variety of tasks at the nanoscale.
基金supported by the National Key Research and Development Program of China(2020YFA0908200)the National Natural Science Foundation of China(52073060 and 52073060)the Natural Science Foundation of Jiangsu(BE2018707).
文摘Micro/nanoscale motors(MNMs)have been regarded as promising tools in the field of engineered regeneration due to unique property of autonomous motion.Herein,a review on the advancements of MNMs in the area of engineered regeneration is presented,covering aspects from their propulsion mechanisms to their frontiers in engineered regeneration,listing the revolutionary applications in biosensing,medical imaging,drug delivery and tissue engineering.Finally,challenges and future directions of MNMs are finally discussed on the basis of the achievements.
