WRKY transcription factors play key roles in plant resistance to various stresses,but their roles in fruit ripening remain largely unknown.Here,we report a WRKY gene PpWRKY14 involved in the regulation of fruit ripeni...WRKY transcription factors play key roles in plant resistance to various stresses,but their roles in fruit ripening remain largely unknown.Here,we report a WRKY gene PpWRKY14 involved in the regulation of fruit ripening in peach.The expression of PpWRKY14 showed an increasing trend throughout fruit development.PpWRKY14was a target gene of PpNAC1,a master regulator of peach fruit ripening.PpWRKY14 could directly bind to the promoters of PpACS1 and PpACO1 to induce their expression,and this induction was greatly enhanced when PpWRKY14 formed a dimer with PpNAC1.However,the transcription of PpNAC1 could be directly suppressed by two EIN3/EIL1 genes,PpEIL2 and PpEIL3.The PpEIL2/3 genes were highly expressed at the early stages of fruit development,but their expression was programmed to decrease significantly during the ripening stage,thus derepressing the expression of PpNAC1.These results suggested a PpEIL2/3–PpNAC1–PpWRKY14 module that regulates fruit ripening by modulating ethylene production in peach.Our results provided an insight into the regulatory roles of EIN3/EIL1 and WRKY genes in fruit ripening.展开更多
A 1050 bp up-stream regulatory fragment of the transcription factor gene NAC1in Arabidopsis thaliana was isolated using polymerase chain reaction (PCR) based techniques. Thefragment was used to substitute the 35S prom...A 1050 bp up-stream regulatory fragment of the transcription factor gene NAC1in Arabidopsis thaliana was isolated using polymerase chain reaction (PCR) based techniques. Thefragment was used to substitute the 35S promoter of the pBI121 plasmid to construct abate-glucuronidase gene (GUS) expression system. The construct was introduced into tobacco(Nico-tiana tabaccum) plants by the Agrobacterium-med\aled transferring method. GUS expressionpattern was studied by using the transgenic lines. The results showed that the GUS driven by theNAC1 up-stream regulatory region was specifically expressed in the root meristem region, basal areasof the lateral root primordium and the lateral roots. The GUS expression was induced by3-indolebutyric acid (IBA)and gibberellins (GA_3 and GA_(4+7)). The results indicated that theup-stream regulatory fragment of NAC1 responded to plant hormones. The fragment might be involved inboth auxins and gibberellins signaling in promoting the development of lateral roots.展开更多
基金supported by funds received from the National Natural Science Foundation of China(U23A20206,32102363,and 32272687)the Key Special Project of Intergovernmental International Cooperation of the National Key R&D Program of China(2023YFE0125100)+1 种基金the China Agriculture Research System(CARS-30)Hubei Hongshan Laboratory(2021hszd017)。
文摘WRKY transcription factors play key roles in plant resistance to various stresses,but their roles in fruit ripening remain largely unknown.Here,we report a WRKY gene PpWRKY14 involved in the regulation of fruit ripening in peach.The expression of PpWRKY14 showed an increasing trend throughout fruit development.PpWRKY14was a target gene of PpNAC1,a master regulator of peach fruit ripening.PpWRKY14 could directly bind to the promoters of PpACS1 and PpACO1 to induce their expression,and this induction was greatly enhanced when PpWRKY14 formed a dimer with PpNAC1.However,the transcription of PpNAC1 could be directly suppressed by two EIN3/EIL1 genes,PpEIL2 and PpEIL3.The PpEIL2/3 genes were highly expressed at the early stages of fruit development,but their expression was programmed to decrease significantly during the ripening stage,thus derepressing the expression of PpNAC1.These results suggested a PpEIL2/3–PpNAC1–PpWRKY14 module that regulates fruit ripening by modulating ethylene production in peach.Our results provided an insight into the regulatory roles of EIN3/EIL1 and WRKY genes in fruit ripening.
基金supported by the National Natural Science Foundation of China(Grant No.30200169)the Specialized Research Fund for the Doctoral Program of Higher Education(Grant No.20010019008).
文摘A 1050 bp up-stream regulatory fragment of the transcription factor gene NAC1in Arabidopsis thaliana was isolated using polymerase chain reaction (PCR) based techniques. Thefragment was used to substitute the 35S promoter of the pBI121 plasmid to construct abate-glucuronidase gene (GUS) expression system. The construct was introduced into tobacco(Nico-tiana tabaccum) plants by the Agrobacterium-med\aled transferring method. GUS expressionpattern was studied by using the transgenic lines. The results showed that the GUS driven by theNAC1 up-stream regulatory region was specifically expressed in the root meristem region, basal areasof the lateral root primordium and the lateral roots. The GUS expression was induced by3-indolebutyric acid (IBA)and gibberellins (GA_3 and GA_(4+7)). The results indicated that theup-stream regulatory fragment of NAC1 responded to plant hormones. The fragment might be involved inboth auxins and gibberellins signaling in promoting the development of lateral roots.
