向日葵是重要的耐旱、耐盐碱油料作物,其产量与品质对地区经济有重要影响。NAC转录因子是植物中最大的蛋白家族之一,广泛参与植物多种生命活动,并在植物响应非生物与生物胁迫中起重要作用。为揭示向日葵NAC转录因子(HaNAC)密码子的使用...向日葵是重要的耐旱、耐盐碱油料作物,其产量与品质对地区经济有重要影响。NAC转录因子是植物中最大的蛋白家族之一,广泛参与植物多种生命活动,并在植物响应非生物与生物胁迫中起重要作用。为揭示向日葵NAC转录因子(HaNAC)密码子的使用模式和形成原因,利用CUSP、CodonW等软件程序对124个HaNAC基因进行密码子使用模式分析。结果表明,HaNAC基因密码子第1(GC1)、第2(GC2)和第3位碱基(GC3)的GC含量表现为GC1>GC3>GC2,且均低于50%,表明向日葵HaNAC基因密码子偏好使用A/T。有效密码子数(effective number of codons,ENC)表明,HaNAC基因密码子偏好性弱。密码子适应指数(codon adaptation index,CAI)表明,HaNAC基因表达水平低。向日葵NAC基因密码子偏好性受自然选择影响较大,突变压力次之。在HaNAC基因密码子中AGA使用频率最高,共有29个高频密码子和20个最优密码子,且最优密码子偏好A/T结尾。综上,向日葵HaNAC基因密码子使用以A/T碱基为主且偏好A/T结尾,其密码子偏好性主要受自然选择驱动,且偏好性较弱、基因表达水平较低。以上结果为向日葵NAC转录因子的密码子优化、偏好机制及遗传进化研究提供了参考。展开更多
Tiller number is a crucial agronomic trait for achieving high yield in rice.NAC proteins play critical roles in regulating plant growth and development.However,the role of NAC transcription factors in regulating tille...Tiller number is a crucial agronomic trait for achieving high yield in rice.NAC proteins play critical roles in regulating plant growth and development.However,the role of NAC transcription factors in regulating tiller number remains poorly understood.In this study,we isolated a rice NAC gene,OsNAC022,which is conserved in cereal crops and functions as a transcriptional activator.To investigate the role of this gene in rice,we used CRISPR/Cas9 technology to generate a homozygous mutant named CR-osnac022,which exhibited increased tiller number.展开更多
文摘向日葵是重要的耐旱、耐盐碱油料作物,其产量与品质对地区经济有重要影响。NAC转录因子是植物中最大的蛋白家族之一,广泛参与植物多种生命活动,并在植物响应非生物与生物胁迫中起重要作用。为揭示向日葵NAC转录因子(HaNAC)密码子的使用模式和形成原因,利用CUSP、CodonW等软件程序对124个HaNAC基因进行密码子使用模式分析。结果表明,HaNAC基因密码子第1(GC1)、第2(GC2)和第3位碱基(GC3)的GC含量表现为GC1>GC3>GC2,且均低于50%,表明向日葵HaNAC基因密码子偏好使用A/T。有效密码子数(effective number of codons,ENC)表明,HaNAC基因密码子偏好性弱。密码子适应指数(codon adaptation index,CAI)表明,HaNAC基因表达水平低。向日葵NAC基因密码子偏好性受自然选择影响较大,突变压力次之。在HaNAC基因密码子中AGA使用频率最高,共有29个高频密码子和20个最优密码子,且最优密码子偏好A/T结尾。综上,向日葵HaNAC基因密码子使用以A/T碱基为主且偏好A/T结尾,其密码子偏好性主要受自然选择驱动,且偏好性较弱、基因表达水平较低。以上结果为向日葵NAC转录因子的密码子优化、偏好机制及遗传进化研究提供了参考。
基金supported by the National Natural Science Foundation of China(Grant No.32372083)the Fundamental Research Funds for Central Public Welfare Research Institutes of Chinese Rice Research Institute(Grant No.CPSIBRF-CNRRI-202401)+1 种基金the Strategic Cooperation Project between Chongqing Municipal People’s Government and Chinese Academy of Agricultural Sciences‘Introduction,Excavation and Innovative Utilization of High-quality Rice Resources’,the China Agriculture Research System(Grant No.CARS-01-14)the Agricultural Science and Technology Innovation Program,China(Grant No.CAAS‐ASTIP‐2013‐CNRRI).
文摘Tiller number is a crucial agronomic trait for achieving high yield in rice.NAC proteins play critical roles in regulating plant growth and development.However,the role of NAC transcription factors in regulating tiller number remains poorly understood.In this study,we isolated a rice NAC gene,OsNAC022,which is conserved in cereal crops and functions as a transcriptional activator.To investigate the role of this gene in rice,we used CRISPR/Cas9 technology to generate a homozygous mutant named CR-osnac022,which exhibited increased tiller number.
