Long-term manure application has the potential to alleviate soil acidification, and increase carbon sequestration and nutrient availability, thus improving cropland fertility. However, the mechanisms behind greenhouse...Long-term manure application has the potential to alleviate soil acidification, and increase carbon sequestration and nutrient availability, thus improving cropland fertility. However, the mechanisms behind greenhouse gas N_(2)O emissions from acidic soil mediated by long-term manure application remain poorly understood. Herein, we investigated N_(2)O emission and its linkage with gross N mineralization and nitrification rates, as well as nitrifying and denitrifying microbes in an acidic upland soil subjected to 36-year fertilization treatments, including an unfertilized control(CK), inorganic fertilizer(F), 2× rate of inorganic fertilizer(2F), manure(M), and the combination of inorganic fertilizer and manure(FM) treatments. Compared to the CK treatment(1.34 μg N kg^(-1) d^(-1)), fertilization strongly increased N_(2)O emissions by 34-fold on average, with more pronounced increases in the manure-amendment(10.6-169 μg N kg^(-1) d^(-1)) than those in the inorganic fertilizer treatments(3.26-5.51 μg N kg^(-1) d^(-1)). The manure amendment-stimulated N_(2)O emissions were highly associated with increased soil pH, mean weight diameter of soil aggregates, substrate availability(e.g., particulate organic carbon, NO_(3)^(-)and available phosphorus), gross N mineralization rates, denitrifier abundances and the(nirK+nirS)/nosZ ratio. These findings suggest that the increased N_(2)O emissions primarily resulted from alleviated acidification, increased substrate availability and improved soil structure, thus enhancing microbial N mineralization and favoring N_(2)O^(-)producing denitrifiers over N_(2)O consumers. Moreover, ammonia-oxidizing bacteria(AOB) rather than ammonia-oxidizing archaea(AOA) positively correlated with soil NO_(3)^(-)concentration and N_(2)O emissions, indicating that nitrification indirectly contributed to N_(2)O production by supplying NO_(3)^(-)for denitrification. Collectively, manure amendment potentially stimulates N_(2)O emissions, primarily resulting from alleviated soil acidification and increased substrate availability, thus enhancing N mineralization and denitrifier-mediated N_(2)O production. Our findings suggest that consideration should be given to the greenhouse gas budgets of agricultural ecosystems when applying manure for managing the pH and fertility of acidic soils.展开更多
[Objective] The aim was to investigate and reveal effect of measurement time on emission flux of CO2 and N2O to ensure the optimum time of emission flux, in order to provide scientific reference for emission reduction...[Objective] The aim was to investigate and reveal effect of measurement time on emission flux of CO2 and N2O to ensure the optimum time of emission flux, in order to provide scientific reference for emission reduction of greenhouse gas in black soil region. [Method] Based on experiment of long-term fertilizer location in black soil region, the paper studied on daily dynamic variation of CO2 and N2O discharge in 3 key growth periods （booting stage, grain-filling stage and mature stage） to reveal differences of CO2 and N2O emission flux in different times. [Result] Daily variations of CO2 and N2O emission flux were large, from 205 mg/（m2·h） to 552 mg/（m2·h） for CO2 and from 51 h to 295 μg/（m2·h） for N2O. Trend of CO2 discharge in different growth times showed a unimodal curve, and the peak was at noon of 12：00 and the peak valley was at 3：00 am; discharge of N2O was small in day time at booting stage and large at night. Regardless of rice growth period effect on CO2 and N2O emission flux, representative time of CO2 discharge was 6：00-8：00 or 16：00-21：00; and time of N2O was 8：00-10：00 or 16：00-21：00; if CO2 and N2O emission fluxes were measured simultaneously, the optimum time was 16：00-18：00; if the measurement was started during 9：00-12：00, correction coefficients of CO2 and N2O were 0.81 and 0.90, respectively. [Conclusion] The result provided scientific reference for reduction of greenhouse gas emission in black soil region.展开更多
Urban areas are the major anthropogenic source of atmospheric CO_(2),thus making longterm and continuous observations of their carbon emission dynamics extremely important.The COVID-19 lockdown served as a natural exp...Urban areas are the major anthropogenic source of atmospheric CO_(2),thus making longterm and continuous observations of their carbon emission dynamics extremely important.The COVID-19 lockdown served as a natural experiment that provided a unique opportunity to analyse the contribution of human activities to CO_(2) emissions from urban areas.In 2020,Beijing experienced COVID-19 confinement with different levels of restrictions on social mobility and economic activity,resulting in reductions in CO_(2) emissions.To investigate the response mechanisms of CO_(2) flux to restriction measures,we analysed CO_(2) flux data obtained using the eddy covariance technique from 2015 to 2020,and compared CO_(2) flux during the COVID-19 confinement period in 2020 with the preceding years(2015-2019)and across various levels of confinement.The results showed that:(1)the annual CO_(2) flux was 2.1±0.2 kg C/(m^(2)·yr)in 2020,which showed a significant reduction of 31.8%compared to the adjacent 2019;(2)the reduction in CO_(2) flux was closely related to the level of restrictions on human activities;(3)most reductions occurred during the morning(85.7%)and evening(32.7%)peak traffic times,indicating that commuting-related transportation is a primary contributor to urban CO_(2) emissions.It is suggested that measures that reduce transportation-related CO_(2) sources should be considered as priorities for reducing urban CO_(2) emissions.The dynamic variation of urban CO_(2) flux captured by the eddy covariance technology is conductive to strengthening the supervision of the implementation of urban carbon emission reduction policies,promoting the achievement of dual carbon goals.展开更多
The response of N_(2)O emissions to nitrogen(N)addition is usually positive,but its response to phosphorus(P)addition varies,and the underlying mechanisms for the changes in N_(2)O emissions remain unclear.We conducte...The response of N_(2)O emissions to nitrogen(N)addition is usually positive,but its response to phosphorus(P)addition varies,and the underlying mechanisms for the changes in N_(2)O emissions remain unclear.We conducted field studies to examine the response of N_(2)O emissions to N and P addition over two years in three typical alpine grasslands,alpine meadow(AM),alpine steppe(AS),and alpine cultivated grassland(CG)on the Qinghai-Tibet Plateau(QTP).Our results showed consistent increases in N_(2)O emissions under N addition alone or with P addition,and insignificant change in N_(2)O emissions under P addition alone in all three grasslands.N addition increased N_(2)O emissions directly in AM,by lowering soil pH in AS,and by lowering abundance of denitrification genes in CG.N and P co-addition increased N_(2)O emissions in AM and AS but only showed an interactive effect in AM.P addition enhanced the increase in N_(2)O emissions caused by N addition mainly by promoting plant growth in AM.Overall,our results illustrate that short-term P addition cannot alleviate the stimulation of N_(2)O emissions by N deposition in alpine grassland ecosystems,and may even further stimulate N_(2)O emissions.展开更多
A series of Eu^(2+)/Eu^(3+)doped 20La_(2)O_(3)-20Al_(2)O_(3)-60SiO_(2)glasses(LAS:Eu)were fabricated via melting quenching method in air atmosphere.By introducing the reducing agent Si_(3)N_(4),the ratio of Eu^(2+)/Eu...A series of Eu^(2+)/Eu^(3+)doped 20La_(2)O_(3)-20Al_(2)O_(3)-60SiO_(2)glasses(LAS:Eu)were fabricated via melting quenching method in air atmosphere.By introducing the reducing agent Si_(3)N_(4),the ratio of Eu^(2+)/Eu^(3+)in glasses can be controlled under atmospheric conditions at 1520℃for 5 h.As the tunable Eu^(2+)/Eu^(3+)component in LAS:Eu glasses,the wavelength conversion of photoluminescence is achieved upon the395 nm excitation,where LAS:0.7Eu exhibits a color coordinate of(0.334,0.314).According to calculation,the energy transfer mechanism between Eu^(2+)and Eu^(3+)in glasses is dipole-dipole interactions dominate.Meanwhile,relative X-ray excited luminescence(XEL)intensity of the single Eu^(2+)doped glass can reach 38.6%of that of Bi_(4)Ge_(3)O_(12)(BGO)crystal.The temperature-dependent emission spectra of the LAS:Eu glasses were tested under photoluminescence and X-ray excitation,and the thermal activation energy was calculated.These results demonstrate the potential of LAS:Eu glass for applications as lightemitting diode(LED)materials and scintillators in nuclear radiation detection.展开更多
The soil type is a key factor influencing N(nitrogen)cycling in soil;however,gross N transformations and N_(2)O emission sources are still poorly understood.In this study,a laboratory 15N tracing experiment was carrie...The soil type is a key factor influencing N(nitrogen)cycling in soil;however,gross N transformations and N_(2)O emission sources are still poorly understood.In this study,a laboratory 15N tracing experiment was carried out at 60%WHC(water holding capacity)and 25℃to evaluate the gross N transformation rates and N_(2)O emission pathways in sandy loam and silt loam soils in a semi-arid region of Heilongjiang Province,China.The results showed that the gross rates of N mineralization,immobilization,and nitrification were 3.60,1.90,and 5.63 mg N/(kg·d)in silt loam soil,respectively,which were 3.62,4.26,and 3.13 times those in sandy loam soil,respectively.The ratios of the gross nitrification rate to the ammonium immobilization rate(n/ia)in sandy loam soil and silt loam soil were all higher than 1.00,whereas the n/ia in sandy loam soil(4.36)was significantly higher than that in silt loam soil(3.08).This result indicated that the ability of sandy loam soil to release and conserve the available N was relatively poor in comparison with silt loam soil,and the relatively strong nitrification rate compared to the immobilization rate may lead to N loss through NO_(3)–leaching.Under aerobic conditions,both nitrification and denitrification made contributions to N_(2)O emissions.Nitrification was the dominant pathway leading to N_(2)O production in soils and was responsible for 82.0%of the total emitted N_(2)O in sandy loam soil,which was significantly higher than that in silt loam soil(71.7%).However,the average contribution of denitrification to total N_(2)O production in sandy loam soil was 17.9%,which was significantly lower than that in silt loam soil(28.3%).These results are valuable for developing reasonable fertilization management and proposing effective greenhouse gas mitigation strategies in different soil types in semiarid regions.展开更多
With the increasing application of anammox for the treatment of high-strength industrial wastewater,application of anammox in municipal sewage has been gaining more attention.Sludge granulation in particular enhances ...With the increasing application of anammox for the treatment of high-strength industrial wastewater,application of anammox in municipal sewage has been gaining more attention.Sludge granulation in particular enhances the enrichment and retention of anammox bacteria in municipal sewage treatment systems.However,the performance of granular sludge under continuous and varying hydraulic loading shock remains little understood.In this study,the robustness of anammox granular sludge in treating lowstrength municipal sewage under various shock loadings was investigated.Results showed that an upflow anaerobic sludge blanket(UASB)reactor with anammox granules performed well,with anammox specific activity up to 0.28 kg N/kg VSS/day and anti-loading shock capability up to 187.2 L/day during the 8-month testing period.The accumulation rate of N2O(<0.01 kg N/kg VSS/day)in the liquid phase was seven times higher than that of the gas phase,which could be mainly attributed to the incomplete denitrification and insufficient carbon source.However,only a small part of the produced N2O escaped into the atmosphere.High-throughput sequencing and molecular ecological network analyses also identified the bacterial diversity and community structure,indicating the potential resistance against loading shock.The composition and structural analyses showed that polysaccharides were an important functional component in the tightly bound extracellular polymeric substances(TB-EPS),which was the major EPS layer of anammox granules.Scanning electron microscopy(SEM)also showed that the gaps in between the anammoxclusters in the granules inhibit the flotation of the sludge and ensure efficient settling and retention of anammox granules.展开更多
A split-plot experiment in a rice-winter wheat rotation system was performed to study the effects of water regime and wheat straw application in rice-growing season on N2O emission from following wheat growing season....A split-plot experiment in a rice-winter wheat rotation system was performed to study the effects of water regime and wheat straw application in rice-growing season on N2O emission from following wheat growing season. Water regime in the rice-growing season was designed as the conventional irrigation (flooding/drainage cycle) and the permanent flooding. Wheat straw was incorporated with three rates of 0, 225 and 450 g m-2 into the paddy soil for each water regime just before rice was transplanted. N2O emission was measured by static chamber-gas chromatograph method. Results from the variance analysis indicated that the permanent flooding in rice-growing season markedly enhanced N2O emission in following wheat growing season (P=0. 003), and that the effect of straw application on N2O emission was distinguished between two water regimes. Under the conventional irrigation, incoporation of wheat straw reduced N2O emission in the following wheat growing season, while there were no significant differences in the emission for the straw application rates of 225 and 450 g m-2. No significant differences in N2O emissions were observed among the three rates of straw application for the permanent flooding regime. In addition, the seasonal variation of N2O emission was regulated by soil temperature and moisture. The daily N2O flux (Y, mg m-2 d-1) can be quantitatively described by soil temperature (T, ℃) and moisture (W, WFPS %) asY=A0+A1T+A2W+A3W2(n=23, R2 ≥0. 4159** )or y=C0+C1W+C2W2(n=23,R2≥0. 4074** ). Compared with the effect of soil temperature on N2O emission, soil moisture was an important factor regulating the seasonal pattern of N2O emission.展开更多
Reclamation of degraded grasslands as managed grasslands has been increasingly accelerated in recent years in China. Land use change affects soil nitrogen（N） dynamics and nitrous oxide（N2O） emissions. However, it ...Reclamation of degraded grasslands as managed grasslands has been increasingly accelerated in recent years in China. Land use change affects soil nitrogen（N） dynamics and nitrous oxide（N2O） emissions. However, it remains unclear how large-scale grassland reclamation will impact the grassland ecosystem as a whole. Here, we investigated the effects of the conversion from native to managed grasslands on soil N dynamics and N2O emissions by field experiments in Hulunber in northern China. Soil（0-10 cm）, nitrate（NO3-）,ammonium（NH4＋）, and microbial N were measured in plots in a temperate steppe（Leymus chinensis grassland） and two managed grasslands（Medicago sativa and Bromus inermis grasslands） in 2011 and 2012. The results showed conversion of L. chinensis grassland to M.sativa or B. inermis grasslands decreased concentrations of NO3--N, but did not change NH4-N . Soil microbial N was slightly decreased by the conversion of L. chinensis grassland to M.sativa, but increased by the conversion to B. inermis. The conversion of L. chinensis grassland to M. sativa（i.e., a legume grass） increased N2O emissions by 26.2%, while the conversion to the B. inermis（i.e., a non-legume grass） reduced N2O emissions by 33.1%. The conversion from native to managed grasslands caused large created variations in soil NO3-＋-N and NH4-N concentrations. Net N mineralization rates did not change significantly in growing season or vegetation type, but to net nitrification rate. These results provide evidence on how reclamation may impact the grassland ecosystem in terms of N dynamics and N2O emissions.展开更多
The Yellow River Delta Wetland is one of the youngest wetlands, and also the most complete, extensive wetlands in China. The wetland in this delta is ecologically important due to their hydrologic attributes and their...The Yellow River Delta Wetland is one of the youngest wetlands, and also the most complete, extensive wetlands in China. The wetland in this delta is ecologically important due to their hydrologic attributes and their roles as ecotones between terrestrial and aquatic ecosystems. In the study, the spatial and temporal variation characteristics of CH4 and CO2 emission flux under five kinds of land use types in the wetland were investigated. The results indicated that the greenhouse gas emission flux, especially the CO2 and CH4, showed distinctly spatial and temporal variation under different land use types in the wetland. In the spring, the emission flux of CO2 was higher than that of CO2 in the autumn, and appeared negative in HW3 and HW4 in the autumn. CH4 emission flux of HW4 and HW5 was negative in the spring and autumn, which indicated that the CH4 emission process was net absorption. Among the five kinds of land use types, the CO2 emission flux of HW4 discharged the largest emission flux reaching 29.3 mg.m-2.h-1, but the CH4 emission flux of HW2 discharged the largest emission flux reaching 0.15 mg.m-2.h-1. From the estuary to the inland, the emission flux of CO2 was decreased at first and then appeared increasing trend, but the emission flux of CH4 was contrary to CO2.展开更多
Tree species and temperature change arising from seasonal variation or global warming are two important factors influencing N2O and NO emissions from forest soils. However, few studies have examined the effects of tem...Tree species and temperature change arising from seasonal variation or global warming are two important factors influencing N2O and NO emissions from forest soils. However, few studies have examined the effects of temperatures （5-35℃） on the emissions of forest soil N2O and NO in typical subtropical region. A short-term laboratory experiment was carried out to investigate the influence of temperature changes （5-35℃） on soil N2O and NO emissions under aerobic conditions in two contrasting （broad-leaved and coniferous） subtropical acidic forest types in China. The results showed that the temporal pattern of N2O and NO emissions between the three lower temperatures （5℃, 15℃, and 25℃） and 35℃ was significantly different for both broad-leaved and coniferous forest soils. The effects of temperature on soil N2O and NO emission rates varied between broad-leaved and coniferous forest soils. Both N2O and NO emissions increased exponentially with an increase in temperature in the broad-leaved forest soil. However, N2O and NO emissions in the coniferous forest soil were not sensitive to temperature change between 5℃ and 25℃. N2O and NO emission rates were significantly higher in the broad-leaved forest soil as compared with the coniferous forest soil at all incubation temperatures except 5℃. These results suggest that the broad-leaved forest could contribute more N2O and NO emissions than the coniferous forest for most of the year in the subtropical region of China.展开更多
Tropical peat swamp forest beds that have been reclaimed for agricultural use are generally an active source of nitrous oxide （N2O） efflux, however, the mechanism by which reclaimed tropical peat soils promote the e...Tropical peat swamp forest beds that have been reclaimed for agricultural use are generally an active source of nitrous oxide （N2O） efflux, however, the mechanism by which reclaimed tropical peat soils promote the emergence of N2O emitters in soil microbial communities remains unclear. The purpose of this study was to reveal the vertical distribution of N2O emission potential and its correlation with mineral nitrogen contents in reclaimed soils. Using a culture-based N2O emission assay, the N2O emission potentials of soil at various depths （0-450 cm） were investigated in two oil palm plantations in Sarawak, Malaysia, which had elapsed times of two years （E2Y） and 10 years （El 0Y） after deforestation, respectively. On the basis of the relationship between the vertical profiles of N2O emission potentials and the contents of mineralized nitrogen in the peat soils at various depths, the impact of land management on soil microbial communities was discussed. The peat soil at plantation site E2Y showed a trend of high N2O production in deep layers （200-400 cm）, whereas the older plantation site E10Y showed considerably more active N2O emission in shallow soil （10-50 cm）. N2O emission potentials among the soil microbial communities at different soil depths at the E10Y site showed positive correlations with NO3- and NH4＋ contents, whereas, soils obtained from the E2Y site had N2O emission potentials that were inversely proportional to the contents of NO3-. This contrasting vertical correlation between N2O-emitting potentials and mineralized nitrogen contents in bulk soils suggests that active N2O emission in deep soil at the E2Y site has maintained the original carbon-nitrogen （C/N） ratio of the peat soil, whereas at EIOY, such a regulatory system has been lost due to advanced soil degradation, leading to dynamic changes in the nitrogen cycle in shallow soil.展开更多
To understand influence of litters on the emission/absorption of CO2, N2O and CH4 in broadleaved/Korean pine forest in Changbai Mountain, fluxes of soil CO2, N2O and CH4 were measured by closed static chamber techniqu...To understand influence of litters on the emission/absorption of CO2, N2O and CH4 in broadleaved/Korean pine forest in Changbai Mountain, fluxes of soil CO2, N2O and CH4 were measured by closed static chamber technique, from Sept 3, 2002 to Oct 30, 2003 in two types of soil ecosystems, of which one was covered with litters on the surface soil, and the other had no litters. The results showed that litters had significant influences on CO2, N2O and CH4 fluxes (p<0.05). Their diurnal change patterns of plot with litters and litter-free plot were similar, and they all showed emission/absorption peak at 18:00. The diurnal change fluxes of CO2 and N2O of plot with litters were significantly higher than those of the litter-free plot, while the diurnal flux of CH4 of plot with litters was lower than that of litter-free plot. The fluxes of CO2, N2O, and CH4 showed the similar seasonal patternsfor both plots. The fluxes of CO2, CH4 showed their peak fluxes in June, but the fluxes of N2O showed its peak emissions in August. The annual fluxes of CO2 and N2O of plot with litters were significantly higher than those of the litter-free plot, while the annual flux of CH4 of plot with litters was lower than that of litter-free plot. Keywords Flux - CO2, N2O and CH4 - Seasonal variation - Diurnal variation CLC number S718.5 Document code A Foundation item: The study was supported by innovation research project of Institute of Appiied Ecology, Chinese Academy of Sciences (SCXZD0101-02) and National Natural Science Foundation of China (30271068)Bigraphy: XIAO Dong-mei (1979-), female, master of Institute of Applied Ecology. Chinese Academy of Sciences, Shenyang 110016. P. R. China.Responsible editor: Song Funan展开更多
Soil samples were taken from depth of 0-12 cm in virgin broad-leaved Korean pine mixed forest in Changbai Moun-tain in July 2000. The effects of temperature, soil water content, pH, NH4+ and NO3- on N2O emission and C...Soil samples were taken from depth of 0-12 cm in virgin broad-leaved Korean pine mixed forest in Changbai Moun-tain in July 2000. The effects of temperature, soil water content, pH, NH4+ and NO3- on N2O emission and CH4 uptake of a for-est soil were studied in laboratory by the method of orthogonal design. It was observed under laboratory conditions in this study that there were significant correlations between N2O emission rate, CH4 oxidation rate, soil pH and temperature. Nevertheless, N2O emission rate also showed a significant positive correlation with CH4 oxidation rate. The results suggested that pH and temperature were important factors controlling N2O emission and CH4 oxidation under this experiment conditions.展开更多
This experiment was conducted in Xinxiang, Henan from June 2013 to June 2014. Total four treatments were designed including farmers ’ common practice （F, 250 kg/hm^2）, 80% F （LF, 200 kg/hm^2）, 80% F＋biochar （LF...This experiment was conducted in Xinxiang, Henan from June 2013 to June 2014. Total four treatments were designed including farmers ’ common practice （F, 250 kg/hm^2）, 80% F （LF, 200 kg/hm^2）, 80% F＋biochar （LFC） and no fertilizer （CK） to measure the dynamic emissions of CO2 and N2O from a summer maize-winter wheat field by static chamber-gas chromatography method. The results showed that the soil CO2 emission was 21.8-1 022.7 mg/（m^2·h）, and was mainly influenced by soil temperature and moisture content. During the growth of summer maize, the soil CO2 emission was more significantly affected by soil moisture con-tent; and in winter wheat growing season, it was more significantly affected by soil temperature in the top 5 cm. The LF and LFC treatments significantly reduced the soil cumulative CO2 emission, especial y during the growth of winter wheat. Fertiliza-tion and irrigation were the main factors influencing the soil N2O emission. The soil N2O emission during the fertilization period accounted for 73.9%-74.5% and 40.5%-43.6% of the soil cumulative N2O emission during the summer maize-and winter wheat-growing season, respectively. The peak of emission fluxes was determined by fertilization amount, while the occurrence time of emission peak and emission re-duction effect were influenced by irrigation. The LF treatment reduced the soil cu-mulative N2O emission by 15.7%-16.8% and 18.1%-18.5% during the growth period of summer maize and winter wheat, respectively. Reduced nitrogen fertilization is an effective way for reducing N2O emission in intensive high-yielding farmland. Under a suitable nitrogen level （200 kg/hm^2）, the application of biochar showed no significant effect on the soil N2O emission in a short term. The N2O emission factors of the L and LF treatments were 0.60% and 0.56%, respectively. ln the intensive high-yield-ing farmland of North China, reducing the nitrogen application amount is an appro-priate measure to mitigate greenhouse gas emissions without crop yield loss.展开更多
In this study, Bacil us amyloliquefaciens A3 was continual y incubated in shake fIasks contalning wastewater from sweet potato starch production as an ef-fective biofertiIizer for cuItivation of Brassica juncea var. m...In this study, Bacil us amyloliquefaciens A3 was continual y incubated in shake fIasks contalning wastewater from sweet potato starch production as an ef-fective biofertiIizer for cuItivation of Brassica juncea var. multiceps（XueIihong）. Based on pot experiments in the greenhouse, the effects of chemical fertiIizers （CN）, biofertiIizer （BF）, inactivated broth （BI）, starch wastewater （SW） and the combination of biofertiIizer and chemical fertiIizer （BC） on the yield, NO3- content and NO2- con-tent of XueIihong, soiI physicochemical properties and N2O emission were investi-gated. The resuIts showed that the yield of XueIihong in BC and CN treatments was improved by five times compared with CK; BF and SW treatments had insignifi-cant impact on the yield of XueIihong. Compared with CN treatment, BCL treatment exhibited simiIar improving effects on the yield of XueIihong, in which NO3- content of XueIihong and soiI was reduced by 16.4%-73.6% and 22%-29%, which reduced the risk of nitrogen eIuviations in soiI; average N2O fIux （FPV30） in BCL treatment was reduced by 58.3%-73.1% compared with CN treatment. In concIusion, B. amy-loliquefaciens is a feasibIe Iow-cost biofertiIizer for sustalnabIe vegetabIe farming with a great potential for starch wastewater utiIization.展开更多
基金financially supported by the National Science & Technology Fundamental Resources Investigation Project of China (2021FY100501)the Youth Innovation of Chinese Academy of Agricultural Sciences (Y2023QC16)。
文摘Long-term manure application has the potential to alleviate soil acidification, and increase carbon sequestration and nutrient availability, thus improving cropland fertility. However, the mechanisms behind greenhouse gas N_(2)O emissions from acidic soil mediated by long-term manure application remain poorly understood. Herein, we investigated N_(2)O emission and its linkage with gross N mineralization and nitrification rates, as well as nitrifying and denitrifying microbes in an acidic upland soil subjected to 36-year fertilization treatments, including an unfertilized control(CK), inorganic fertilizer(F), 2× rate of inorganic fertilizer(2F), manure(M), and the combination of inorganic fertilizer and manure(FM) treatments. Compared to the CK treatment(1.34 μg N kg^(-1) d^(-1)), fertilization strongly increased N_(2)O emissions by 34-fold on average, with more pronounced increases in the manure-amendment(10.6-169 μg N kg^(-1) d^(-1)) than those in the inorganic fertilizer treatments(3.26-5.51 μg N kg^(-1) d^(-1)). The manure amendment-stimulated N_(2)O emissions were highly associated with increased soil pH, mean weight diameter of soil aggregates, substrate availability(e.g., particulate organic carbon, NO_(3)^(-)and available phosphorus), gross N mineralization rates, denitrifier abundances and the(nirK+nirS)/nosZ ratio. These findings suggest that the increased N_(2)O emissions primarily resulted from alleviated acidification, increased substrate availability and improved soil structure, thus enhancing microbial N mineralization and favoring N_(2)O^(-)producing denitrifiers over N_(2)O consumers. Moreover, ammonia-oxidizing bacteria(AOB) rather than ammonia-oxidizing archaea(AOA) positively correlated with soil NO_(3)^(-)concentration and N_(2)O emissions, indicating that nitrification indirectly contributed to N_(2)O production by supplying NO_(3)^(-)for denitrification. Collectively, manure amendment potentially stimulates N_(2)O emissions, primarily resulting from alleviated soil acidification and increased substrate availability, thus enhancing N mineralization and denitrifier-mediated N_(2)O production. Our findings suggest that consideration should be given to the greenhouse gas budgets of agricultural ecosystems when applying manure for managing the pH and fertility of acidic soils.
基金Supported by Chinese National Natural Science Foundation(41001138)National Science and Technology Supporting Program(2009BADB3B04)~~
文摘[Objective] The aim was to investigate and reveal effect of measurement time on emission flux of CO2 and N2O to ensure the optimum time of emission flux, in order to provide scientific reference for emission reduction of greenhouse gas in black soil region. [Method] Based on experiment of long-term fertilizer location in black soil region, the paper studied on daily dynamic variation of CO2 and N2O discharge in 3 key growth periods （booting stage, grain-filling stage and mature stage） to reveal differences of CO2 and N2O emission flux in different times. [Result] Daily variations of CO2 and N2O emission flux were large, from 205 mg/（m2·h） to 552 mg/（m2·h） for CO2 and from 51 h to 295 μg/（m2·h） for N2O. Trend of CO2 discharge in different growth times showed a unimodal curve, and the peak was at noon of 12：00 and the peak valley was at 3：00 am; discharge of N2O was small in day time at booting stage and large at night. Regardless of rice growth period effect on CO2 and N2O emission flux, representative time of CO2 discharge was 6：00-8：00 or 16：00-21：00; and time of N2O was 8：00-10：00 or 16：00-21：00; if CO2 and N2O emission fluxes were measured simultaneously, the optimum time was 16：00-18：00; if the measurement was started during 9：00-12：00, correction coefficients of CO2 and N2O were 0.81 and 0.90, respectively. [Conclusion] The result provided scientific reference for reduction of greenhouse gas emission in black soil region.
基金supported by the National Key Research Program of China(No.2017YFE0127700)the National Natural Science Foundation of China(No.42301325)China Postdoctoral Science Foundation(Nos.2023M743704 and 2024T170975)。
文摘Urban areas are the major anthropogenic source of atmospheric CO_(2),thus making longterm and continuous observations of their carbon emission dynamics extremely important.The COVID-19 lockdown served as a natural experiment that provided a unique opportunity to analyse the contribution of human activities to CO_(2) emissions from urban areas.In 2020,Beijing experienced COVID-19 confinement with different levels of restrictions on social mobility and economic activity,resulting in reductions in CO_(2) emissions.To investigate the response mechanisms of CO_(2) flux to restriction measures,we analysed CO_(2) flux data obtained using the eddy covariance technique from 2015 to 2020,and compared CO_(2) flux during the COVID-19 confinement period in 2020 with the preceding years(2015-2019)and across various levels of confinement.The results showed that:(1)the annual CO_(2) flux was 2.1±0.2 kg C/(m^(2)·yr)in 2020,which showed a significant reduction of 31.8%compared to the adjacent 2019;(2)the reduction in CO_(2) flux was closely related to the level of restrictions on human activities;(3)most reductions occurred during the morning(85.7%)and evening(32.7%)peak traffic times,indicating that commuting-related transportation is a primary contributor to urban CO_(2) emissions.It is suggested that measures that reduce transportation-related CO_(2) sources should be considered as priorities for reducing urban CO_(2) emissions.The dynamic variation of urban CO_(2) flux captured by the eddy covariance technology is conductive to strengthening the supervision of the implementation of urban carbon emission reduction policies,promoting the achievement of dual carbon goals.
基金funded by the National Key R&D Program of China(2021YFE0112400 and 2023YFF1304303)the National Natural Science Foundation of China(32361143870 and 32101315)。
文摘The response of N_(2)O emissions to nitrogen(N)addition is usually positive,but its response to phosphorus(P)addition varies,and the underlying mechanisms for the changes in N_(2)O emissions remain unclear.We conducted field studies to examine the response of N_(2)O emissions to N and P addition over two years in three typical alpine grasslands,alpine meadow(AM),alpine steppe(AS),and alpine cultivated grassland(CG)on the Qinghai-Tibet Plateau(QTP).Our results showed consistent increases in N_(2)O emissions under N addition alone or with P addition,and insignificant change in N_(2)O emissions under P addition alone in all three grasslands.N addition increased N_(2)O emissions directly in AM,by lowering soil pH in AS,and by lowering abundance of denitrification genes in CG.N and P co-addition increased N_(2)O emissions in AM and AS but only showed an interactive effect in AM.P addition enhanced the increase in N_(2)O emissions caused by N addition mainly by promoting plant growth in AM.Overall,our results illustrate that short-term P addition cannot alleviate the stimulation of N_(2)O emissions by N deposition in alpine grassland ecosystems,and may even further stimulate N_(2)O emissions.
基金Project supported by"Pioneer"and"Leading Goose"R&D Program of Zhejiang(2022C01046)the National Natural Science Foundation of China(12375183,61505193,12275262,11975220,51972291)Natural Science Foundation of Zhejiang(LGG22E020001)。
文摘A series of Eu^(2+)/Eu^(3+)doped 20La_(2)O_(3)-20Al_(2)O_(3)-60SiO_(2)glasses(LAS:Eu)were fabricated via melting quenching method in air atmosphere.By introducing the reducing agent Si_(3)N_(4),the ratio of Eu^(2+)/Eu^(3+)in glasses can be controlled under atmospheric conditions at 1520℃for 5 h.As the tunable Eu^(2+)/Eu^(3+)component in LAS:Eu glasses,the wavelength conversion of photoluminescence is achieved upon the395 nm excitation,where LAS:0.7Eu exhibits a color coordinate of(0.334,0.314).According to calculation,the energy transfer mechanism between Eu^(2+)and Eu^(3+)in glasses is dipole-dipole interactions dominate.Meanwhile,relative X-ray excited luminescence(XEL)intensity of the single Eu^(2+)doped glass can reach 38.6%of that of Bi_(4)Ge_(3)O_(12)(BGO)crystal.The temperature-dependent emission spectra of the LAS:Eu glasses were tested under photoluminescence and X-ray excitation,and the thermal activation energy was calculated.These results demonstrate the potential of LAS:Eu glass for applications as lightemitting diode(LED)materials and scintillators in nuclear radiation detection.
基金financed by the National Natural Science Foundation of China(41301345,41101284)。
文摘The soil type is a key factor influencing N(nitrogen)cycling in soil;however,gross N transformations and N_(2)O emission sources are still poorly understood.In this study,a laboratory 15N tracing experiment was carried out at 60%WHC(water holding capacity)and 25℃to evaluate the gross N transformation rates and N_(2)O emission pathways in sandy loam and silt loam soils in a semi-arid region of Heilongjiang Province,China.The results showed that the gross rates of N mineralization,immobilization,and nitrification were 3.60,1.90,and 5.63 mg N/(kg·d)in silt loam soil,respectively,which were 3.62,4.26,and 3.13 times those in sandy loam soil,respectively.The ratios of the gross nitrification rate to the ammonium immobilization rate(n/ia)in sandy loam soil and silt loam soil were all higher than 1.00,whereas the n/ia in sandy loam soil(4.36)was significantly higher than that in silt loam soil(3.08).This result indicated that the ability of sandy loam soil to release and conserve the available N was relatively poor in comparison with silt loam soil,and the relatively strong nitrification rate compared to the immobilization rate may lead to N loss through NO_(3)–leaching.Under aerobic conditions,both nitrification and denitrification made contributions to N_(2)O emissions.Nitrification was the dominant pathway leading to N_(2)O production in soils and was responsible for 82.0%of the total emitted N_(2)O in sandy loam soil,which was significantly higher than that in silt loam soil(71.7%).However,the average contribution of denitrification to total N_(2)O production in sandy loam soil was 17.9%,which was significantly lower than that in silt loam soil(28.3%).These results are valuable for developing reasonable fertilization management and proposing effective greenhouse gas mitigation strategies in different soil types in semiarid regions.
基金financially supported by the National Natural Science Foundation of China (Nos. 21707155, 41671471, 41322012 and 91851204)the Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDB15020303)+4 种基金the National Key R&D Program (No. 2016YFA0602303)the Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program (No. 2017BT01Z176)the special fund from the State Key Joint Laboratory of Environment Simulation and Pollution Control (Research Center for Eco-environmental Sciences, Chinese Academy of Sciences) (No. 18Z02ESPCR)the support of a Humboldt Research Fellowship (No. 1152633)Program of the Youth Innovation Promotion Association (CAS)
文摘With the increasing application of anammox for the treatment of high-strength industrial wastewater,application of anammox in municipal sewage has been gaining more attention.Sludge granulation in particular enhances the enrichment and retention of anammox bacteria in municipal sewage treatment systems.However,the performance of granular sludge under continuous and varying hydraulic loading shock remains little understood.In this study,the robustness of anammox granular sludge in treating lowstrength municipal sewage under various shock loadings was investigated.Results showed that an upflow anaerobic sludge blanket(UASB)reactor with anammox granules performed well,with anammox specific activity up to 0.28 kg N/kg VSS/day and anti-loading shock capability up to 187.2 L/day during the 8-month testing period.The accumulation rate of N2O(<0.01 kg N/kg VSS/day)in the liquid phase was seven times higher than that of the gas phase,which could be mainly attributed to the incomplete denitrification and insufficient carbon source.However,only a small part of the produced N2O escaped into the atmosphere.High-throughput sequencing and molecular ecological network analyses also identified the bacterial diversity and community structure,indicating the potential resistance against loading shock.The composition and structural analyses showed that polysaccharides were an important functional component in the tightly bound extracellular polymeric substances(TB-EPS),which was the major EPS layer of anammox granules.Scanning electron microscopy(SEM)also showed that the gaps in between the anammoxclusters in the granules inhibit the flotation of the sludge and ensure efficient settling and retention of anammox granules.
基金This work was supported by the Hundred Talents Program launched by the Chinese Academy of Sciencesthe National Key Basic Research Development Foundation of China(G1999011805).
文摘A split-plot experiment in a rice-winter wheat rotation system was performed to study the effects of water regime and wheat straw application in rice-growing season on N2O emission from following wheat growing season. Water regime in the rice-growing season was designed as the conventional irrigation (flooding/drainage cycle) and the permanent flooding. Wheat straw was incorporated with three rates of 0, 225 and 450 g m-2 into the paddy soil for each water regime just before rice was transplanted. N2O emission was measured by static chamber-gas chromatograph method. Results from the variance analysis indicated that the permanent flooding in rice-growing season markedly enhanced N2O emission in following wheat growing season (P=0. 003), and that the effect of straw application on N2O emission was distinguished between two water regimes. Under the conventional irrigation, incoporation of wheat straw reduced N2O emission in the following wheat growing season, while there were no significant differences in the emission for the straw application rates of 225 and 450 g m-2. No significant differences in N2O emissions were observed among the three rates of straw application for the permanent flooding regime. In addition, the seasonal variation of N2O emission was regulated by soil temperature and moisture. The daily N2O flux (Y, mg m-2 d-1) can be quantitatively described by soil temperature (T, ℃) and moisture (W, WFPS %) asY=A0+A1T+A2W+A3W2(n=23, R2 ≥0. 4159** )or y=C0+C1W+C2W2(n=23,R2≥0. 4074** ). Compared with the effect of soil temperature on N2O emission, soil moisture was an important factor regulating the seasonal pattern of N2O emission.
基金supported by The National Basic Research Program (973) of China (No. 2015CB150800)the National Key Research and Development Program of China (No. 2016YFC0500603)+1 种基金the China Agriculture Research System “China agriculture research system” (No. CARS-35)the National Nonprofit Institute Research Grant of CAAS (No. 647-53)
文摘Reclamation of degraded grasslands as managed grasslands has been increasingly accelerated in recent years in China. Land use change affects soil nitrogen（N） dynamics and nitrous oxide（N2O） emissions. However, it remains unclear how large-scale grassland reclamation will impact the grassland ecosystem as a whole. Here, we investigated the effects of the conversion from native to managed grasslands on soil N dynamics and N2O emissions by field experiments in Hulunber in northern China. Soil（0-10 cm）, nitrate（NO3-）,ammonium（NH4＋）, and microbial N were measured in plots in a temperate steppe（Leymus chinensis grassland） and two managed grasslands（Medicago sativa and Bromus inermis grasslands） in 2011 and 2012. The results showed conversion of L. chinensis grassland to M.sativa or B. inermis grasslands decreased concentrations of NO3--N, but did not change NH4-N . Soil microbial N was slightly decreased by the conversion of L. chinensis grassland to M.sativa, but increased by the conversion to B. inermis. The conversion of L. chinensis grassland to M. sativa（i.e., a legume grass） increased N2O emissions by 26.2%, while the conversion to the B. inermis（i.e., a non-legume grass） reduced N2O emissions by 33.1%. The conversion from native to managed grasslands caused large created variations in soil NO3-＋-N and NH4-N concentrations. Net N mineralization rates did not change significantly in growing season or vegetation type, but to net nitrification rate. These results provide evidence on how reclamation may impact the grassland ecosystem in terms of N dynamics and N2O emissions.
文摘The Yellow River Delta Wetland is one of the youngest wetlands, and also the most complete, extensive wetlands in China. The wetland in this delta is ecologically important due to their hydrologic attributes and their roles as ecotones between terrestrial and aquatic ecosystems. In the study, the spatial and temporal variation characteristics of CH4 and CO2 emission flux under five kinds of land use types in the wetland were investigated. The results indicated that the greenhouse gas emission flux, especially the CO2 and CH4, showed distinctly spatial and temporal variation under different land use types in the wetland. In the spring, the emission flux of CO2 was higher than that of CO2 in the autumn, and appeared negative in HW3 and HW4 in the autumn. CH4 emission flux of HW4 and HW5 was negative in the spring and autumn, which indicated that the CH4 emission process was net absorption. Among the five kinds of land use types, the CO2 emission flux of HW4 discharged the largest emission flux reaching 29.3 mg.m-2.h-1, but the CH4 emission flux of HW2 discharged the largest emission flux reaching 0.15 mg.m-2.h-1. From the estuary to the inland, the emission flux of CO2 was decreased at first and then appeared increasing trend, but the emission flux of CH4 was contrary to CO2.
基金This work was supported by the National Natural Science Foundation of China （No. 41301238, 41222005）, and the National Water Pollution Control and Management Technology Major Projects of China （No. 2011ZX07101004）.
文摘Tree species and temperature change arising from seasonal variation or global warming are two important factors influencing N2O and NO emissions from forest soils. However, few studies have examined the effects of temperatures （5-35℃） on the emissions of forest soil N2O and NO in typical subtropical region. A short-term laboratory experiment was carried out to investigate the influence of temperature changes （5-35℃） on soil N2O and NO emissions under aerobic conditions in two contrasting （broad-leaved and coniferous） subtropical acidic forest types in China. The results showed that the temporal pattern of N2O and NO emissions between the three lower temperatures （5℃, 15℃, and 25℃） and 35℃ was significantly different for both broad-leaved and coniferous forest soils. The effects of temperature on soil N2O and NO emission rates varied between broad-leaved and coniferous forest soils. Both N2O and NO emissions increased exponentially with an increase in temperature in the broad-leaved forest soil. However, N2O and NO emissions in the coniferous forest soil were not sensitive to temperature change between 5℃ and 25℃. N2O and NO emission rates were significantly higher in the broad-leaved forest soil as compared with the coniferous forest soil at all incubation temperatures except 5℃. These results suggest that the broad-leaved forest could contribute more N2O and NO emissions than the coniferous forest for most of the year in the subtropical region of China.
文摘Tropical peat swamp forest beds that have been reclaimed for agricultural use are generally an active source of nitrous oxide （N2O） efflux, however, the mechanism by which reclaimed tropical peat soils promote the emergence of N2O emitters in soil microbial communities remains unclear. The purpose of this study was to reveal the vertical distribution of N2O emission potential and its correlation with mineral nitrogen contents in reclaimed soils. Using a culture-based N2O emission assay, the N2O emission potentials of soil at various depths （0-450 cm） were investigated in two oil palm plantations in Sarawak, Malaysia, which had elapsed times of two years （E2Y） and 10 years （El 0Y） after deforestation, respectively. On the basis of the relationship between the vertical profiles of N2O emission potentials and the contents of mineralized nitrogen in the peat soils at various depths, the impact of land management on soil microbial communities was discussed. The peat soil at plantation site E2Y showed a trend of high N2O production in deep layers （200-400 cm）, whereas the older plantation site E10Y showed considerably more active N2O emission in shallow soil （10-50 cm）. N2O emission potentials among the soil microbial communities at different soil depths at the E10Y site showed positive correlations with NO3- and NH4＋ contents, whereas, soils obtained from the E2Y site had N2O emission potentials that were inversely proportional to the contents of NO3-. This contrasting vertical correlation between N2O-emitting potentials and mineralized nitrogen contents in bulk soils suggests that active N2O emission in deep soil at the E2Y site has maintained the original carbon-nitrogen （C/N） ratio of the peat soil, whereas at EIOY, such a regulatory system has been lost due to advanced soil degradation, leading to dynamic changes in the nitrogen cycle in shallow soil.
基金The study was supported by innovation research project of Institute of Applied Ecology Chinese Academy of Sciences (SCXZD0101-02) and National Natural Science Foundation of China (302
文摘To understand influence of litters on the emission/absorption of CO2, N2O and CH4 in broadleaved/Korean pine forest in Changbai Mountain, fluxes of soil CO2, N2O and CH4 were measured by closed static chamber technique, from Sept 3, 2002 to Oct 30, 2003 in two types of soil ecosystems, of which one was covered with litters on the surface soil, and the other had no litters. The results showed that litters had significant influences on CO2, N2O and CH4 fluxes (p<0.05). Their diurnal change patterns of plot with litters and litter-free plot were similar, and they all showed emission/absorption peak at 18:00. The diurnal change fluxes of CO2 and N2O of plot with litters were significantly higher than those of the litter-free plot, while the diurnal flux of CH4 of plot with litters was lower than that of litter-free plot. The fluxes of CO2, N2O, and CH4 showed the similar seasonal patternsfor both plots. The fluxes of CO2, CH4 showed their peak fluxes in June, but the fluxes of N2O showed its peak emissions in August. The annual fluxes of CO2 and N2O of plot with litters were significantly higher than those of the litter-free plot, while the annual flux of CH4 of plot with litters was lower than that of litter-free plot. Keywords Flux - CO2, N2O and CH4 - Seasonal variation - Diurnal variation CLC number S718.5 Document code A Foundation item: The study was supported by innovation research project of Institute of Appiied Ecology, Chinese Academy of Sciences (SCXZD0101-02) and National Natural Science Foundation of China (30271068)Bigraphy: XIAO Dong-mei (1979-), female, master of Institute of Applied Ecology. Chinese Academy of Sciences, Shenyang 110016. P. R. China.Responsible editor: Song Funan
基金This paper was supported by Chinese Academy of Sciences.
文摘Soil samples were taken from depth of 0-12 cm in virgin broad-leaved Korean pine mixed forest in Changbai Moun-tain in July 2000. The effects of temperature, soil water content, pH, NH4+ and NO3- on N2O emission and CH4 uptake of a for-est soil were studied in laboratory by the method of orthogonal design. It was observed under laboratory conditions in this study that there were significant correlations between N2O emission rate, CH4 oxidation rate, soil pH and temperature. Nevertheless, N2O emission rate also showed a significant positive correlation with CH4 oxidation rate. The results suggested that pH and temperature were important factors controlling N2O emission and CH4 oxidation under this experiment conditions.
基金Supported by National Key Technology Research and Development Program(2013BAD11B03)National Natural Science Foundation(31272249,31071865,41505100)~~
文摘This experiment was conducted in Xinxiang, Henan from June 2013 to June 2014. Total four treatments were designed including farmers ’ common practice （F, 250 kg/hm^2）, 80% F （LF, 200 kg/hm^2）, 80% F＋biochar （LFC） and no fertilizer （CK） to measure the dynamic emissions of CO2 and N2O from a summer maize-winter wheat field by static chamber-gas chromatography method. The results showed that the soil CO2 emission was 21.8-1 022.7 mg/（m^2·h）, and was mainly influenced by soil temperature and moisture content. During the growth of summer maize, the soil CO2 emission was more significantly affected by soil moisture con-tent; and in winter wheat growing season, it was more significantly affected by soil temperature in the top 5 cm. The LF and LFC treatments significantly reduced the soil cumulative CO2 emission, especial y during the growth of winter wheat. Fertiliza-tion and irrigation were the main factors influencing the soil N2O emission. The soil N2O emission during the fertilization period accounted for 73.9%-74.5% and 40.5%-43.6% of the soil cumulative N2O emission during the summer maize-and winter wheat-growing season, respectively. The peak of emission fluxes was determined by fertilization amount, while the occurrence time of emission peak and emission re-duction effect were influenced by irrigation. The LF treatment reduced the soil cu-mulative N2O emission by 15.7%-16.8% and 18.1%-18.5% during the growth period of summer maize and winter wheat, respectively. Reduced nitrogen fertilization is an effective way for reducing N2O emission in intensive high-yielding farmland. Under a suitable nitrogen level （200 kg/hm^2）, the application of biochar showed no significant effect on the soil N2O emission in a short term. The N2O emission factors of the L and LF treatments were 0.60% and 0.56%, respectively. ln the intensive high-yield-ing farmland of North China, reducing the nitrogen application amount is an appro-priate measure to mitigate greenhouse gas emissions without crop yield loss.
基金Supported by Key Research Program of the Chinese Academy of Sciences(KZZD-EW-09-3,KZZD-EW-11-03)National Science and Technology Major Project(2014ZX07204-005)Special Fund of the National Academy Alliance(2012-1)~~
文摘In this study, Bacil us amyloliquefaciens A3 was continual y incubated in shake fIasks contalning wastewater from sweet potato starch production as an ef-fective biofertiIizer for cuItivation of Brassica juncea var. multiceps（XueIihong）. Based on pot experiments in the greenhouse, the effects of chemical fertiIizers （CN）, biofertiIizer （BF）, inactivated broth （BI）, starch wastewater （SW） and the combination of biofertiIizer and chemical fertiIizer （BC） on the yield, NO3- content and NO2- con-tent of XueIihong, soiI physicochemical properties and N2O emission were investi-gated. The resuIts showed that the yield of XueIihong in BC and CN treatments was improved by five times compared with CK; BF and SW treatments had insignifi-cant impact on the yield of XueIihong. Compared with CN treatment, BCL treatment exhibited simiIar improving effects on the yield of XueIihong, in which NO3- content of XueIihong and soiI was reduced by 16.4%-73.6% and 22%-29%, which reduced the risk of nitrogen eIuviations in soiI; average N2O fIux （FPV30） in BCL treatment was reduced by 58.3%-73.1% compared with CN treatment. In concIusion, B. amy-loliquefaciens is a feasibIe Iow-cost biofertiIizer for sustalnabIe vegetabIe farming with a great potential for starch wastewater utiIization.
