The exploration and understanding of myxosporean,one of the crucial stressors causing fish disease,have always been highly regarded.The present study aims to fully understand a novel myxobolid species,Thelohanellus xi...The exploration and understanding of myxosporean,one of the crucial stressors causing fish disease,have always been highly regarded.The present study aims to fully understand a novel myxobolid species,Thelohanellus xiushanensis n.sp.infecting the gill filaments of Carassius auratus from Chongqing,China,as well as the associated pathological impacts.A multifaceted analysis at various levels and perspectives was carried out by the approach integrating morphology,molecular systematics,histopathology,combined host and habitat,as well as SSU rDNA data.The results indicated that its mature myxospores displayed a tapering anterior and a blunt-rounded posterior,with a dropshape in front view and melon-seed shape in sutural view in morphology.The outer surface was covered with a transparent membrane sheath in valve view.The dimensions of myxospores were average(19.2±1.1)(17.1-21.0)×(10.0±0.7)(8.7-12.1)×(9.7±0.5)(9.1-10.8)μm.An elliptical polar capsule,containing four to five turns of single polar filament,measures(8.0±0.6)(6.9-9.1)×(6.6±0.3)(6.1-7.1)μm.Its SSU rDNA sequence demonstrated the closest similarity(99.95%)with an actinosporean Neoactinomyxum sp.NEO-7.Histopathological results indicated that numerous myxoplasmodia of T.xiushanensis n.sp.were located in the tips of the gill filaments,resulting in deformation and rupture of partial gill filaments.Furthermore,severely infected gill lamellae exhibited hyperplasia and fusion,accompanied by infiltration of numerous eosinophils.In addition,regenerated gill filaments were observed at the bases of partially ruptured gill filaments,indicating that the host’s gill tissue has the innate capacity to shed diseased tissues and regenerate new ones.This study has proved the present species and Neoactinomyxum sp.NEO-7 belong to the same myxosporean species but corresponding to different developmental stage,designated as a new species.This novel species is a potential pathogen for the host,which should not be ignored.This study not only enriches myxobolid biodiversity and demonstrates a potentially pathogenic agent,but also provides a valid diagnosis for the gill myxoboliosis caused by the present pathogen in aquaculture.展开更多
目的:探讨基于CT三维重组技术的R.E.N.A.L.评分系统在腹腔镜肾部分切除术中的临床应用价值。方法:搜集我院2020年6月至2024年1月经手术治疗的79例肾肿瘤患者的临床及术前影像资料,根据术前肾脏CT三维重组结果,分析血管变异与肾脏热缺血...目的:探讨基于CT三维重组技术的R.E.N.A.L.评分系统在腹腔镜肾部分切除术中的临床应用价值。方法:搜集我院2020年6月至2024年1月经手术治疗的79例肾肿瘤患者的临床及术前影像资料,根据术前肾脏CT三维重组结果,分析血管变异与肾脏热缺血时间、手术总时间、术中出血量及术后并发症之间的关系,并进行R.E.N.A.L.评分。采用Logistic回归筛选出肾热缺血时间大于20 min的独立危险因素。结果:有无血管变异在肾脏热缺血时间、手术总时间、术中出血量及术后并发症之间无明显差异(P > 0.05)。R.E.N.A.L.总评分是肾热缺血时间大于20 min的独立危险因素(P P Objective: To explore the clinical application value of R.E.N.A.L. scoring system based on CT three-dimensional reconstruction technology in laparoscopic partial nephrectomy. Methods: The clinical and preoperative imaging data of 79 patients with renal tumors who underwent surgical treatment in our hospital from June 2020 to January 2024 were collected. According to the results of preoperative renal CT three-dimensional reconstruction, the relationship between vascular variation and renal warm ischemia time, total operation time, intraoperative bleeding and postoperative complications was analyzed, and R.E.N.A.L. score was performed. Logistic regression was used to screen out independent risk factors for renal warm ischemia time greater than 20 min. Results: There was no significant difference in renal warm ischemia time, total operation time, intraoperative blood loss and postoperative complications between the presence or absence of vascular variation (P > 0.05). The total R.E.N.A.L. score was an independent risk factor for renal warm ischemia time greater than 20 min (P P < 0.05). Conclusion: CT three-dimensional reconstruction can clearly understand the anatomical location of the kidney, tumor and blood vessels before surgery, thereby reducing the occurrence of perioperative complications.展开更多
基金National Natural Science Foundation of China(31970409)Projects of Chongqing Science and Technology Commission(cstc2021jcyj-msxmX0731)。
文摘The exploration and understanding of myxosporean,one of the crucial stressors causing fish disease,have always been highly regarded.The present study aims to fully understand a novel myxobolid species,Thelohanellus xiushanensis n.sp.infecting the gill filaments of Carassius auratus from Chongqing,China,as well as the associated pathological impacts.A multifaceted analysis at various levels and perspectives was carried out by the approach integrating morphology,molecular systematics,histopathology,combined host and habitat,as well as SSU rDNA data.The results indicated that its mature myxospores displayed a tapering anterior and a blunt-rounded posterior,with a dropshape in front view and melon-seed shape in sutural view in morphology.The outer surface was covered with a transparent membrane sheath in valve view.The dimensions of myxospores were average(19.2±1.1)(17.1-21.0)×(10.0±0.7)(8.7-12.1)×(9.7±0.5)(9.1-10.8)μm.An elliptical polar capsule,containing four to five turns of single polar filament,measures(8.0±0.6)(6.9-9.1)×(6.6±0.3)(6.1-7.1)μm.Its SSU rDNA sequence demonstrated the closest similarity(99.95%)with an actinosporean Neoactinomyxum sp.NEO-7.Histopathological results indicated that numerous myxoplasmodia of T.xiushanensis n.sp.were located in the tips of the gill filaments,resulting in deformation and rupture of partial gill filaments.Furthermore,severely infected gill lamellae exhibited hyperplasia and fusion,accompanied by infiltration of numerous eosinophils.In addition,regenerated gill filaments were observed at the bases of partially ruptured gill filaments,indicating that the host’s gill tissue has the innate capacity to shed diseased tissues and regenerate new ones.This study has proved the present species and Neoactinomyxum sp.NEO-7 belong to the same myxosporean species but corresponding to different developmental stage,designated as a new species.This novel species is a potential pathogen for the host,which should not be ignored.This study not only enriches myxobolid biodiversity and demonstrates a potentially pathogenic agent,but also provides a valid diagnosis for the gill myxoboliosis caused by the present pathogen in aquaculture.
文摘目的:探讨基于CT三维重组技术的R.E.N.A.L.评分系统在腹腔镜肾部分切除术中的临床应用价值。方法:搜集我院2020年6月至2024年1月经手术治疗的79例肾肿瘤患者的临床及术前影像资料,根据术前肾脏CT三维重组结果,分析血管变异与肾脏热缺血时间、手术总时间、术中出血量及术后并发症之间的关系,并进行R.E.N.A.L.评分。采用Logistic回归筛选出肾热缺血时间大于20 min的独立危险因素。结果:有无血管变异在肾脏热缺血时间、手术总时间、术中出血量及术后并发症之间无明显差异(P > 0.05)。R.E.N.A.L.总评分是肾热缺血时间大于20 min的独立危险因素(P P Objective: To explore the clinical application value of R.E.N.A.L. scoring system based on CT three-dimensional reconstruction technology in laparoscopic partial nephrectomy. Methods: The clinical and preoperative imaging data of 79 patients with renal tumors who underwent surgical treatment in our hospital from June 2020 to January 2024 were collected. According to the results of preoperative renal CT three-dimensional reconstruction, the relationship between vascular variation and renal warm ischemia time, total operation time, intraoperative bleeding and postoperative complications was analyzed, and R.E.N.A.L. score was performed. Logistic regression was used to screen out independent risk factors for renal warm ischemia time greater than 20 min. Results: There was no significant difference in renal warm ischemia time, total operation time, intraoperative blood loss and postoperative complications between the presence or absence of vascular variation (P > 0.05). The total R.E.N.A.L. score was an independent risk factor for renal warm ischemia time greater than 20 min (P P < 0.05). Conclusion: CT three-dimensional reconstruction can clearly understand the anatomical location of the kidney, tumor and blood vessels before surgery, thereby reducing the occurrence of perioperative complications.
