Kinetic studies of the extraction of chromium（Ⅵ）[Cr（Ⅵ）] from the hydrochloric acid solution with tri-n-octylamine（N235） have been made by a Lewis cell. The influences of various kinetic parameters, such as sti...Kinetic studies of the extraction of chromium（Ⅵ）[Cr（Ⅵ）] from the hydrochloric acid solution with tri-n-octylamine（N235） have been made by a Lewis cell. The influences of various kinetic parameters, such as stirring rate, interfacial area, temperature, N235 and initial Cr（Ⅵ） concentrations were evaluated. The rate equations for the extraction of Cr（Ⅵ） by N235 were obtained on the basis of the slope analysis data. The results indicated that the reaction rate of Cr（Ⅵ） increased with increasing of the stirring rate and interfacial area until that a "plateau" was observed with increasing stirring rate. The activation energies of Cr（Ⅵ） extraction by N235 dissolved in diluents were calculated. Kinetics analysis proved that the mass transfer was controlled mainly by diffusion and the chemical reaction between Cr（Ⅵ） anions and N235 could be achieved at the liquid-liquid interface. On the basis of the relation between ln[Cr（Ⅵ）] and ln R0 or ln[N235] and ln R0, the rate equations can be obtained. This research provides a proof-in-concept analysis for kinetic mechanism.展开更多
The extraction kinetics of rhenium(Ⅶ) or molybdenum(Ⅵ) with trialkyl amine (N235, R3N, R=C8–C10) dissolved in heptane were investigated by constant interfacial cell with laminar flow, which aimed to identify the ex...The extraction kinetics of rhenium(Ⅶ) or molybdenum(Ⅵ) with trialkyl amine (N235, R3N, R=C8–C10) dissolved in heptane were investigated by constant interfacial cell with laminar flow, which aimed to identify the extraction regime, reaction zone and rate equations. The influence of stirring speed, temperature, specific interfacial area, extraction concentration and chlorine concentration on the extraction of both metals was studied. It is concluded that the extractions of Re(Ⅶ) and Mo(Ⅵ) both take place at the liquid-liquid interface, while the extraction regimes are chemically-controlled for rhenium and mixed controlled for molybdenum, respectively. The extraction rate equations and the rate-determining step were obtained under the experimental conditions, and the extraction rate constant of Re(Ⅶ) or Mo(Ⅵ) with N235 was calculated. These obtained kinetics parameters are different between Re(Ⅶ) and Mo(Ⅵ), which provides better possibilities for Re(Ⅶ) and Mo(Ⅵ) separations at proper conditions.展开更多
The objective of present study was to investigate the antioxidative and cardioprotective effects of polyphenol extracts from sea buckthorn (PESB) against myocardial ischemia reperfusion injury (MIRI). PESB was analyze...The objective of present study was to investigate the antioxidative and cardioprotective effects of polyphenol extracts from sea buckthorn (PESB) against myocardial ischemia reperfusion injury (MIRI). PESB was analyzed using HPLC-DAD and their antioxidant activities were evaluated by measuring the intrinsic free radicals cavenging capacity and inhibiting the formation of hydroxyl radical capacity. Experiments on the isolated rat hearts were carried out according to the improved Langendorff retrograde perfusion technology. Coronary effluent of lactate dehydrogenase (LDH) and creatine kinase isoenzyme (CK-MB) activities were analyzed to determine the extent of myocardial injury. Protein expression levels of Beclin-1, microtubule-associated protein 1 light chain 3 (LC3) I and II were measured to evaluate the degree of autophagy. In conclusion, coupled results from both in vivo and in vitro experiments have confirmed that PESB has potential health implications in both prevention and amelioration of MIRI, which may be attributed to the inhibition of autophagy.展开更多
Background: Supplementing farm animals diet with functional ingredients may improve the nutritional quality of meat products.Diet composition has been also demonstrated to influence the gene expression with effect on ...Background: Supplementing farm animals diet with functional ingredients may improve the nutritional quality of meat products.Diet composition has been also demonstrated to influence the gene expression with effect on biological processes and pathways.However,the knowledge on the effect of nutrients at the molecular level is scant.In particular,studies on the effects of antioxidants and polyphenols dietary supplementation have been investigated mainly in rodents,and only scarcely in farm animals so far.RNA-Seq with next-generation sequencing is increasingly the method of choice for studying changes in the transcriptome and it has been recently employed also in pig nutrigenomics studies to identify diet-induced changes in gene expression.The present study aimed to investigate the effect of diets enriched with functional ingredients(linseed,vitamin E and plant extracts) on the transcriptome of pig Longissimus thoracis to elucidate the role of these compounds in influencing genes involved in muscle physiology and metabolism compared to a standard diet.Results: Eight hundred ninety-three significant differentially expressed genes(DEGs)(FDR adjusted P-value ≤ 0.05)were detected by RNA-Seq analysis in the three diet comparisons(D2-D1,D3-D1,D4-D1).The functional analysis of DEGs showed that the diet enriched with n-3 PUFA from linseed(D2) mostly downregulated genes in pathways and biological processes(BPs) related to muscle development,contraction,and glycogen metabolism compared to the standard diet.The diet supplemented with linseed and vitamin E/Selenium(D3) showed to mostly downregulate genes linked to oxidative phosphorylation.Only few genes involved in extracellular matrix(ECM)organization were upregulated by the D3.Finally,the comparison D4-D1 showed that the diet supplemented with linseed and plant extracts(D4) upregulated the majority of genes compared to D1 that were involved in a complex network of pathways and BPs all connected by hub genes.In particular,IGF2 was a hub gene connecting protein metabolism,ECM organization,immune system and lipid biosynthesis pathways.Conclusion: The supplementation of pig diet with n-3 PUFA from linseed,antioxidants and plant-derived polyphenols can influence the expression of a relevant number of genes in Longissimus thoracis muscle that are involved in a variety of biochemical pathways linked to muscle function and metabolism.展开更多
A coupled reaction-solvent extraction process was used to remove HC1 from a simulated distiller waste. The extraction performances of various extractants and diluents were compared and the apparent basicity ofN235 （a...A coupled reaction-solvent extraction process was used to remove HC1 from a simulated distiller waste. The extraction performances of various extractants and diluents were compared and the apparent basicity ofN235 （a mixture of tertiary amines） in various diluents was determined. The best results were obtained using N235 and isoamyl alcohol as the extractant and diluent, respectively. The yield of HC1 from the coupled extraction was 75% with this extraction system. The mechanisms for the removal of HC1 in both the direct and coupled extractions were investigated. For the coupled extraction, the formation of an RaNHC1 ion-pair complex was involved in the HC1 removal. For the direct extraction, the mechanism involved the formation of hydrogen bonds at high concentrations of HC1.展开更多
基金Natural Science Foundation of the Jiangsu Higher Education Institutions of China(No.18KJD610001)Doctor of Philosophy(PhD)Start-up Foundation of Changzhou Vocational Institute of Engineering(No.11130900117002)+1 种基金Jiangsu Students’Platform for Innovation and Entrepreneurship Training Program,China(No.201813102026H)Qing-Lan Project of Jiangsu Province,China(No.2018-12)
文摘Kinetic studies of the extraction of chromium（Ⅵ）[Cr（Ⅵ）] from the hydrochloric acid solution with tri-n-octylamine（N235） have been made by a Lewis cell. The influences of various kinetic parameters, such as stirring rate, interfacial area, temperature, N235 and initial Cr（Ⅵ） concentrations were evaluated. The rate equations for the extraction of Cr（Ⅵ） by N235 were obtained on the basis of the slope analysis data. The results indicated that the reaction rate of Cr（Ⅵ） increased with increasing of the stirring rate and interfacial area until that a "plateau" was observed with increasing stirring rate. The activation energies of Cr（Ⅵ） extraction by N235 dissolved in diluents were calculated. Kinetics analysis proved that the mass transfer was controlled mainly by diffusion and the chemical reaction between Cr（Ⅵ） anions and N235 could be achieved at the liquid-liquid interface. On the basis of the relation between ln[Cr（Ⅵ）] and ln R0 or ln[N235] and ln R0, the rate equations can be obtained. This research provides a proof-in-concept analysis for kinetic mechanism.
基金Project(20701017) supported by the National Natural Science Foundation of China
文摘The extraction kinetics of rhenium(Ⅶ) or molybdenum(Ⅵ) with trialkyl amine (N235, R3N, R=C8–C10) dissolved in heptane were investigated by constant interfacial cell with laminar flow, which aimed to identify the extraction regime, reaction zone and rate equations. The influence of stirring speed, temperature, specific interfacial area, extraction concentration and chlorine concentration on the extraction of both metals was studied. It is concluded that the extractions of Re(Ⅶ) and Mo(Ⅵ) both take place at the liquid-liquid interface, while the extraction regimes are chemically-controlled for rhenium and mixed controlled for molybdenum, respectively. The extraction rate equations and the rate-determining step were obtained under the experimental conditions, and the extraction rate constant of Re(Ⅶ) or Mo(Ⅵ) with N235 was calculated. These obtained kinetics parameters are different between Re(Ⅶ) and Mo(Ⅵ), which provides better possibilities for Re(Ⅶ) and Mo(Ⅵ) separations at proper conditions.
文摘The objective of present study was to investigate the antioxidative and cardioprotective effects of polyphenol extracts from sea buckthorn (PESB) against myocardial ischemia reperfusion injury (MIRI). PESB was analyzed using HPLC-DAD and their antioxidant activities were evaluated by measuring the intrinsic free radicals cavenging capacity and inhibiting the formation of hydroxyl radical capacity. Experiments on the isolated rat hearts were carried out according to the improved Langendorff retrograde perfusion technology. Coronary effluent of lactate dehydrogenase (LDH) and creatine kinase isoenzyme (CK-MB) activities were analyzed to determine the extent of myocardial injury. Protein expression levels of Beclin-1, microtubule-associated protein 1 light chain 3 (LC3) I and II were measured to evaluate the degree of autophagy. In conclusion, coupled results from both in vivo and in vitro experiments have confirmed that PESB has potential health implications in both prevention and amelioration of MIRI, which may be attributed to the inhibition of autophagy.
基金funded by Regione Emilia-Romagna POR-FESR 2014-2020"Innovare la filiera suina mediante la valorizzazione di sottoprodotti vegetali e l’impiego di avanzate tecnologie "omiche" e di processo,per la produzione sostenibile di carne e salumi ad impatto positivo sulla salute"-Green Charcuterie grant n.PG/2015/730542
文摘Background: Supplementing farm animals diet with functional ingredients may improve the nutritional quality of meat products.Diet composition has been also demonstrated to influence the gene expression with effect on biological processes and pathways.However,the knowledge on the effect of nutrients at the molecular level is scant.In particular,studies on the effects of antioxidants and polyphenols dietary supplementation have been investigated mainly in rodents,and only scarcely in farm animals so far.RNA-Seq with next-generation sequencing is increasingly the method of choice for studying changes in the transcriptome and it has been recently employed also in pig nutrigenomics studies to identify diet-induced changes in gene expression.The present study aimed to investigate the effect of diets enriched with functional ingredients(linseed,vitamin E and plant extracts) on the transcriptome of pig Longissimus thoracis to elucidate the role of these compounds in influencing genes involved in muscle physiology and metabolism compared to a standard diet.Results: Eight hundred ninety-three significant differentially expressed genes(DEGs)(FDR adjusted P-value ≤ 0.05)were detected by RNA-Seq analysis in the three diet comparisons(D2-D1,D3-D1,D4-D1).The functional analysis of DEGs showed that the diet enriched with n-3 PUFA from linseed(D2) mostly downregulated genes in pathways and biological processes(BPs) related to muscle development,contraction,and glycogen metabolism compared to the standard diet.The diet supplemented with linseed and vitamin E/Selenium(D3) showed to mostly downregulate genes linked to oxidative phosphorylation.Only few genes involved in extracellular matrix(ECM)organization were upregulated by the D3.Finally,the comparison D4-D1 showed that the diet supplemented with linseed and plant extracts(D4) upregulated the majority of genes compared to D1 that were involved in a complex network of pathways and BPs all connected by hub genes.In particular,IGF2 was a hub gene connecting protein metabolism,ECM organization,immune system and lipid biosynthesis pathways.Conclusion: The supplementation of pig diet with n-3 PUFA from linseed,antioxidants and plant-derived polyphenols can influence the expression of a relevant number of genes in Longissimus thoracis muscle that are involved in a variety of biochemical pathways linked to muscle function and metabolism.
文摘A coupled reaction-solvent extraction process was used to remove HC1 from a simulated distiller waste. The extraction performances of various extractants and diluents were compared and the apparent basicity ofN235 （a mixture of tertiary amines） in various diluents was determined. The best results were obtained using N235 and isoamyl alcohol as the extractant and diluent, respectively. The yield of HC1 from the coupled extraction was 75% with this extraction system. The mechanisms for the removal of HC1 in both the direct and coupled extractions were investigated. For the coupled extraction, the formation of an RaNHC1 ion-pair complex was involved in the HC1 removal. For the direct extraction, the mechanism involved the formation of hydrogen bonds at high concentrations of HC1.
