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温度与时间对BVDV N^(pro)蛋白裂解活性的影响
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作者 任晓婷 安乐乐 +2 位作者 王春林 刘泽煜 马晓霞 《江苏农业学报》 北大核心 2026年第3期598-605,共8页
为探究牛病毒性腹泻病毒(Bovine viral diarrhea virus,BVDV)N^(pro)蛋白酶的自催化裂解活性及其关键调控因素,本研究通过构建含N^(pro)自切割位点的重组融合表达载体pET-28a-N^(pro)-C5-EGFP,采用大肠杆菌BL21(DE3)为工程菌,在不同温度... 为探究牛病毒性腹泻病毒(Bovine viral diarrhea virus,BVDV)N^(pro)蛋白酶的自催化裂解活性及其关键调控因素,本研究通过构建含N^(pro)自切割位点的重组融合表达载体pET-28a-N^(pro)-C5-EGFP,采用大肠杆菌BL21(DE3)为工程菌,在不同温度(16℃、25℃、30℃、37℃)及不同诱导时间(2 h、4 h、6 h、8 h、10 h)条件下进行重组蛋白诱导表达,并利用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)与蛋白质印迹法(Western blot)检测重组蛋白表达情况。结果表明,相对分子量4.54×10^(4)的N^(pro)-C5-EGFP融合蛋白在诱导条件下可自主裂解产生相对分子量为1.85×10^(4)的N^(pro)蛋白,且有完整的自催化活性。裂解率随诱导时间的延长呈显著线性升高趋势;随着温度的增加,裂解率呈先升高后降低的趋势,最适裂解温度为30℃。N^(pro)蛋白含有潜在的活性中心(Cys69-His49催化二元体),具备执行生物功能的空间构象。本研究结果不仅揭示了BVDV N^(pro)蛋白在原核系统表达后仍具有明显的自催化裂解活性,而且揭示了酶学行为受温度和诱导时间的协同调控。本研究结果为进一步研究BVDV N^(pro)的自裂解特性及后续作为抗病毒药物筛选靶点提供了依据。 展开更多
关键词 牛病毒性腹泻病毒 N^(pro) 原核系统 自裂解
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N^(4)-乙酰胞苷修饰在动物生殖发育中的研究进展
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作者 祝新月 胡坤隆 +10 位作者 徐雯睆 轩梦庆 甘信琪 杨智元 王文卓 刘郑秋 张梦雅 周贝贝 俞文杰 张运海 曹祖兵 《遗传》 北大核心 2026年第3期272-286,共15页
N^(4)-乙酰胞苷(N^(4)-acetylcytidine,ac^(4)C)是一类新兴的RNA表观修饰,近年来在表观转录组学研究中被广泛关注。该修饰广泛存在于tRNA、rRNA、mRNA及非编码RNA中,通过精准调控RNA的稳定性与翻译效率,影响下游基因的表达网络,进而对... N^(4)-乙酰胞苷(N^(4)-acetylcytidine,ac^(4)C)是一类新兴的RNA表观修饰,近年来在表观转录组学研究中被广泛关注。该修饰广泛存在于tRNA、rRNA、mRNA及非编码RNA中,通过精准调控RNA的稳定性与翻译效率,影响下游基因的表达网络,进而对细胞功能产生重要影响。本文综述了ac^(4)C修饰的分布特征、生物学功能及复杂调控机制的最新研究进展,详细介绍了其编码器、检测方法以及在动物生殖发育中的作用,旨在为深入探讨ac^(4)C修饰在动物繁殖与人类生殖等领域的调控机制提供坚实的理论基础与研究思路。 展开更多
关键词 N^(4)-乙酰胞苷 RNA修饰 表观遗传 配子成熟 胚胎发育
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猪瘟病毒N^(pro)蛋白的生物信息学分析、原核表达及多克隆抗体制备
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作者 闵开骏 田宇 +2 位作者 张丽媛 罗廷荣 李晓宁 《广西畜牧兽医》 2026年第2期47-52,共6页
通过生物信息学分析并制备猪瘟病毒(Classical swine fever virus,CSFV)N^(pro)蛋白多克隆抗体。采用Alpha Fold2预测N^(pro)蛋白结构,分析蛋白的信号肽、跨膜区、糖基化位点以及亲疏水性来确定抗原片段。以猪瘟病毒石门毒株为模板,PCR... 通过生物信息学分析并制备猪瘟病毒(Classical swine fever virus,CSFV)N^(pro)蛋白多克隆抗体。采用Alpha Fold2预测N^(pro)蛋白结构,分析蛋白的信号肽、跨膜区、糖基化位点以及亲疏水性来确定抗原片段。以猪瘟病毒石门毒株为模板,PCR扩增CSFV N^(pro)基因,克隆到原核表达载体p ET-32a上,构建原核表达载体,诱导和纯化重组蛋白。免疫昆明系小鼠,经4次免疫后采集小鼠血液,分离血清获得N^(pro)蛋白多克隆抗体,测定其特异性和效价。制备出的N^(pro)多克隆抗体能够用于Western Blot检测原核表达的重组蛋白、真核表达的N^(pro)蛋白、病毒蛋白N^(pro)。本结果可为后续深入研究N^(pro)蛋白的生物学功能和猪瘟病毒的致病机制提供实验材料。 展开更多
关键词 猪瘟病毒 N^(pro) 原核表达 多克隆抗体
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N^(4)-乙酰胞苷作为血浆标志物对胃腺癌诊断价值的评估
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作者 吴亚丽 孙金旗 +6 位作者 冀恒涛 李工厂 郭紫琼 朱健宇 曹彤 马卫国 朱立强 《胃肠病学和肝病学杂志》 2026年第3期370-373,共4页
目的探索血浆N^(4)-乙酰胞苷(N^(4)-acetylcytidine,ac4C)对胃腺癌的诊断效能,以为临床提供一种新型的潜在诊断标志物。方法收集经病理确诊的初诊胃腺癌患者和正常人的外周静脉血浆样本,采用ELISA测定血浆ac4C的水平,结合临床病理特征进... 目的探索血浆N^(4)-乙酰胞苷(N^(4)-acetylcytidine,ac4C)对胃腺癌的诊断效能,以为临床提供一种新型的潜在诊断标志物。方法收集经病理确诊的初诊胃腺癌患者和正常人的外周静脉血浆样本,采用ELISA测定血浆ac4C的水平,结合临床病理特征进行ROC曲线分析,系统评估ac4C对胃腺癌的鉴别诊断效能。结果胃腺癌患者血浆ac4C水平[(0.818±0.573)ng/mL]较正常人[(1.917±1.372)ng/mL]显著降低(P<0.0001),且降低幅度与年龄、性别无关。ROC曲线分析显示,ac4C诊断胃腺癌的AUC为0.806(95%CI:0.747~0.865),灵敏度与特异度分别为80.56%和69.81%。血浆ac4C在不同分化及分期胃腺癌患者的AUC为0.790~0.851。结论血浆ac4C在胃腺癌中显著降低,并在不同分期与分化亚组中展现出较好的诊断性能。提示其具有作为一种新型、稳定胃腺癌标志物的潜在价值。 展开更多
关键词 胃腺癌 N^(4)-乙酰胞苷 标志物 血浆
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牛病毒性腹泻病毒非结构蛋白N^(pro)原核表达及多克隆抗体制备
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作者 张书斌 张艺馨 +3 位作者 李英奇 洪菊霜 Uyangaa Temuujin 周建华 《江西农业大学学报》 北大核心 2026年第1期186-195,共10页
【目的】牛病毒性腹泻病毒(BVDV)可严重损害牛群的繁殖性能、呼吸系统、消化功能及泌乳能力。该病毒编码的非结构蛋白N^(pro)具有高度保守性及蛋白酶水解活性,在介导免疫逃逸中发挥关键作用。本研究旨在原核表达BVDV非结构蛋白N^(pro)... 【目的】牛病毒性腹泻病毒(BVDV)可严重损害牛群的繁殖性能、呼吸系统、消化功能及泌乳能力。该病毒编码的非结构蛋白N^(pro)具有高度保守性及蛋白酶水解活性,在介导免疫逃逸中发挥关键作用。本研究旨在原核表达BVDV非结构蛋白N^(pro)及多克隆抗体制备,为深入研究N^(pro)蛋白功能提供物质基础。【方法】通过NCBI网站查找BVDV N^(pro)基因序列,利用相关生物信息学在线软件分析N^(pro)蛋白的理化性质,包括分子量、等电点(pI)、不稳定指数、脂肪族指数及平均亲水性等参数。设计同源重组引物并扩增目的基因N^(pro),克隆至pET-28a(+)载体上,构建重组质粒pET-28a(+)-N^(pro)。将测序正确的pET-28a(+)-N^(pro)质粒转化至E. coli BL21(DE3)表达感受态细胞,经诱导表达、镍柱纯化后的N^(pro)蛋白免疫小鼠进行多克隆抗体制备。采用Western blot鉴定多克隆抗体的反应原性。【结果】(1)信息学分析结果表明,N^(pro)蛋白有504个氨基酸,为胞外亲水性蛋白;N^(pro)蛋白无信号肽及跨膜结构域,二级结构主要由β-折叠和无规卷曲构成。(2)成功构建原核表达质粒pET-28a(+)-N^(pro),经双酶切鉴定及基因测序验证正确后,在大肠杆菌中诱导表达得到分子量约为18 ku重组蛋白N^(pro)。(3)将纯化获取的重组蛋白N^(pro)免疫小鼠制备多克隆抗体,经酶联免疫吸附试验(ELISA)和蛋白质印迹法(Western blot)检测显示,所得到的多克隆抗体效价达1∶409 600,具有良好的特异性、反应原性与免疫原性。【结论】本研究成功在大肠杆菌中高效表达并纯化出具有良好免疫原性的BVDV N^(pro)重组蛋白,所制备的高效价特异性多克隆抗体为深入研究该蛋白的生物学功能提供了有效工具。 展开更多
关键词 牛病毒性腹泻病毒 N^(pro)蛋白 原核表达 多克隆抗体
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Enhanced atomic localization and precision control in a four-level atomic medium via transmission spectrum
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作者 Muhammad Idrees Fatima Ghulam Kakepoto Zareen A Khan 《Communications in Theoretical Physics》 2026年第1期51-60,共10页
In this article,we introduce a new theoretical approach to improve the accuracy of twodimensional(2D)atomic localization within a tripod-type,four-level atomic system by analyzing its transmission spectrum.In this met... In this article,we introduce a new theoretical approach to improve the accuracy of twodimensional(2D)atomic localization within a tripod-type,four-level atomic system by analyzing its transmission spectrum.In this method,the atom interacts with two orthogonal standing-wave fields and a weak probe field.By examining how the weak probe field passes through the system,we can determine the atom position.Our analysis reveals the presence of both double and sharply defined single localized peaks in the transmission spectrum,which correspond to specific positions of the atom.Importantly,we achieve ultra-high-resolution atomic localization with accuracy confined to a region smaller thanλ/32×λ/32.This level of precision is a significant improvement compared to earlier methods,which had lower localization accuracy.The increased precision is due to the complex interaction between the atom and the carefully controlled standing-wave and probe fields,which allows for precise control over the atom's position.The implications of this work are significant,especially for applications like nano-lithography,where precise atomic placement is essential,and for laser cooling technologies,where better atomic localization could lead to more effective cooling processes and improved manipulation of atomic states. 展开更多
关键词 atomic localization transmission spectrum standing-wave fields ultra-high resolution nano-lithography
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Super-resolution reconstruction of UAV-borne gamma-ray spectrum images based on Real-ESRGAN algorithm
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作者 Xin Wang Yuan Yuan +4 位作者 Xuan Zhao Guang-Hao Luo Qi-Qiao Wei He-Xi Wu Chao Xiong 《Nuclear Science and Techniques》 2026年第2期42-54,共13页
Unmanned aerial vehicle(UAV)-borne gamma-ray spectrum survey plays a crucial role in geological mapping,radioactive mineral exploration,and environmental monitoring.However,raw data are often compromised by flight and... Unmanned aerial vehicle(UAV)-borne gamma-ray spectrum survey plays a crucial role in geological mapping,radioactive mineral exploration,and environmental monitoring.However,raw data are often compromised by flight and instrument background noise,as well as detector resolution limitations,which affect the accuracy of geological interpretations.This study aims to explore the application of the Real-ESRGAN algorithm in the super-resolution reconstruction of UAV-borne gamma-ray spectrum images to enhance spatial resolution and the quality of geological feature visualization.We conducted super-resolution reconstruction experiments with 2×,4×and 6×magnification using the Real-ESRGAN algorithm,comparing the results with three other mainstream algorithms(SRCNN,SRGAN,FSRCNN)to verify the superiority in image quality.The experimental results indicate that Real-ESRGAN achieved a structural similarity index(SSIM)value of 0.950 at 2×magnification,significantly higher than the other algorithms,demonstrating its advantage in detail preservation.Furthermore,Real-ESRGAN effectively reduced ringing and overshoot artifacts,enhancing the clarity of geological structures and mineral deposit sites,thus providing high-quality visual information for geological exploration. 展开更多
关键词 UAV-borne gamma-ray spectrum Super-resolution reconstruction Real-ESRGAN Image processing
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Multi-modal data analysis for autism spectrum disorder in children:State of the art and trends
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作者 Lukai Pang Xiaoke Zhao +4 位作者 Lulu Zhao Jianqing Li Fengyi Kuo Hongxing Wang Chengyu Liu 《EngMedicine》 2026年第1期47-56,共10页
Autism spectrum disorder(AsD)is a highly heterogeneous neurodevelopmental disorder.Early diagnosis and intervention are crucial for improving outcomes.Traditional single-modality diagnostic methods are subjective,limi... Autism spectrum disorder(AsD)is a highly heterogeneous neurodevelopmental disorder.Early diagnosis and intervention are crucial for improving outcomes.Traditional single-modality diagnostic methods are subjective,limited,and struggle to reveal the underlying pathological mechanisms.In contrast,multimodal data analysis integrates behavioral,physiological,and neuroimaging information with advanced machine-learning and deeplearning algorithms to overcome these limitations.In this review,we surveyed the recent pediatric AsD literature,highlighting artificial intelligence-driven diagnostic techniques,multimodal data fusion strategies,and emerging trends in ASD assessment.We surveyed studies that integrated two or more modalities and summarized the fusion levels,learning paradigms,tasks,datasets,and metrics.Multimodal approaches outperform singlemodality baselines in classification,severity estimation,and subtyping by leveraging complementary information and reducing modality-specific biases.Multimodal approaches significantly enhance diagnostic accuracy and comprehensiveness,enabling early screening of AsD,symptom subtyping,severity assessment,and personalized interventions.Advances in multimodal fusion techniques have promoted progress in precision medicine for the treatment of ASD. 展开更多
关键词 Autism spectrum disorder Multi-modal data Machine learning Early screening Symptom subtyping
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Generation of ultra-flat broad spectrum with stable single-pulse mode-locking in double-clad Yb-doped fiber laser
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作者 Minghui Sun Dongxin Gao +4 位作者 Yunli Yu Wenyu Wang Qingcao Liu Weixin Liu Yuzhai Pan 《Chinese Physics B》 2026年第1期474-480,共7页
We achieved an ultra-flat broad spectrum output with a 20-dB bandwidth of 77.85 nm in a double-clad Yb-doped fiber laser.The intensity difference between the highest and lowest points of the spectrum indicates a flatn... We achieved an ultra-flat broad spectrum output with a 20-dB bandwidth of 77.85 nm in a double-clad Yb-doped fiber laser.The intensity difference between the highest and lowest points of the spectrum indicates a flatness better than4 dB.More notably,this ultra-flat broad spectrum maintains a stable single-pulse mode-locking state.With the increase of pump power,an ultra-wide spectrum with a 20-dB bandwidth approaching 100 nm was formed at a pump power of 2.25 W.Additionally,we obtained a 9-pulse mode-locked state at another PC station with the same pump,which is the highest number of stable mode-locked pulse bursts observed so far with a first-order Raman frequency shift.This fiber laser shows its benefits of ultra-flat broad spectrum,high stability,and ease of fabrication,which provides a new method of obtaining the broadband light source for multiple practical applications. 展开更多
关键词 ultra-flat broad spectrum high stability double-clad Yb-doped fiber mode-locked multi-pulse bursts
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Equivalent Constant Amplitude Spectrum Design Method and Experimental Verification of Structural Crack Growth Flight⁃by⁃Flight Spectrum
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作者 ZHU Gaoshang ZHAO Qia CAO Jing 《Transactions of Nanjing University of Aeronautics and Astronautics》 2026年第1期85-94,共10页
In fatigue damage tolerance verification tests of aircraft structures,the simulation and loading of flight-byflight spectra require considerable time and resources.To improve the efficiency of load spectrum design and... In fatigue damage tolerance verification tests of aircraft structures,the simulation and loading of flight-byflight spectra require considerable time and resources.To improve the efficiency of load spectrum design and testing,an equivalent constant-amplitude spectrum design method for flight-by-flight spectra is proposed based on the equivalence of crack growth behavior.By combining the Paris crack growth model with the Walker stress ratio correction,the equivalent stress amplitude is directly calculated using structural parameters and load spectrum characteristics,enabling a rapid transformation from variable-amplitude spectra to constant-amplitude spectra.The original spectrum is discretized based on the load-exceedance curve,and the equivalence relationship between multilevel block spectra and constant-amplitude spectra is established.Taking a typical lower wing skin structure of a transport aircraft as an example,two equivalent spectra are designed and validated through fatigue crack growth tests on 2024-T351 center-hole plate specimens.The experimental results show that the fatigue life deviation between the equivalent spectra and the original flight-by-flight spectrum is within 10%,demonstrating the effectiveness of the proposed method.Moreover,the equivalent spectrum constructed under the condition of invariant mean flight stress exhibits higher equivalence accuracy.The influence of spectral shape on the equivalent stress amplitude is further analyzed,revealing that the equivalent stress amplitude increases with the spectrum shape coefficient.The proposed method provides a useful reference for load spectrum design in aircraft structural damage tolerance verification tests. 展开更多
关键词 aircraft structure crack growth fatigue load spectrum equivalent test stress intensity factor
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Exploring patterns and impacts of farmland and construction land upslope in China:based on an integrated slope spectrum and sensitivity model
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作者 ZHOU Yifan CHAI Ji +2 位作者 JIN Gui ZHANG Hongwei LI Qingbiao 《Journal of Mountain Science》 2026年第2期755-774,共20页
As demand for land resources is rapidly growing nowadays,developing on slope lands has become a way to relieve pressure on flat lands.Although some studies use the concept of slope spectrum to explore the trend of lan... As demand for land resources is rapidly growing nowadays,developing on slope lands has become a way to relieve pressure on flat lands.Although some studies use the concept of slope spectrum to explore the trend of land use upslope,relying solely on the slope spectrum is too broad and prevents deeper research.Therefore,using China's land use and DEM data from 2000 to 2020,our study integrated the slope spectrum and the slope sensitivity coefficient(SSC)calculated by the land use transfer matrix as a new approach and method for understanding the underlying formations and impacts of upslope in farmland and construction land,supporting regional management strategies.The results show that:1)Farmlands were upslope in the South and developed horizontally in the North,and construction lands were upslope nationwide.2)Using the land use transfer matrix and SSC,we classified farmland upslope as passive and active patterns,and construction land upslope as saturation and avoidance patterns based on their land use transfer mechanisms in slope space.Provinces with passive and saturation patterns are mainly located near the east coast.3)Different patterns of upslope have distinct impacts on sustainable development.The passive pattern harms food security while the active pattern can relieve pressure on food security but increases ecological risks.Saturation pattern damages food security,ecological protection,and city livability,but avoidance pattern can promote food security and ecological protection.The findings will serve as an essential reference for developing land use strategies aimed at sustainable development. 展开更多
关键词 Slope spectrum Land use transfer Slope climbing Sustainable land use Spatial heterogeneity Sensitivity
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ARQ–UCB:A Reinforcement-Learning Framework for Reliability-Aware and Efficient Spectrum Access in Vehicular IoT
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作者 Adeel Iqbal Tahir Khurshaid +2 位作者 Syed Abdul Mannan Kirmani Mohammad Arif Muhammad Faisal Siddiqui 《Computers, Materials & Continua》 2026年第5期1524-1545,共22页
Vehicular Internet ofThings(V-IoT)networks need intelligent and adaptive spectrum access methods for ensuring ultra-reliable and low-latency communication(URLLC)in highly dynamic environments.Traditional reinforcement... Vehicular Internet ofThings(V-IoT)networks need intelligent and adaptive spectrum access methods for ensuring ultra-reliable and low-latency communication(URLLC)in highly dynamic environments.Traditional reinforcement learning(RL)-based algorithms,such as Q-Learning and Double Q-Learning,are often characterized by unstable convergence and inefficient exploration in the presence of stochastic vehicular traffic and interference.This paper proposes Adaptive Reinforcement Q-learning with Upper Confidence Bound(ARQ-UCB),a lightweight and reliability-aware RL framework,which explicitly reduces interruption and blocking probabilities while improving throughput and delay across diverse vehicular traffic conditions.This proposed ARQ-UCB algorithm extends the basic Q-updates with an exploration confidence term able to dynamically balance exploration and exploitation based on uncertainty estimates,hence allowing faster convergence in case of bursty vehicular traffic.A comprehensive simulation framework evaluates throughput,delay,fairness,energy efficiency,and computational complexity in several V-IoT scenarios.Obtained results indicate that ARQ–UCB attains substantial gains in terms of throughput,fairness,and blocking/delay probabilities while retaining sub-20μs decision latency and O(1)complexity per decision,thus validating real-time feasibility for reliable spectrum access in 5G and beyond V-IoT networks. 展开更多
关键词 V-IoT RL Q-LEARNING upper confidence bound spectrum access URLLC 5G/6G
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3D Spectrum Mapping and Reconstruction Under Multi-Radiation Source Scenarios
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作者 Wang Jie Lin Zhipeng +5 位作者 Zhu Qiuming Wu Qihui Lan Tianxu Zhao Yi Bai Yunpeng Zhong Weizhi 《China Communications》 2026年第2期20-34,共15页
Spectrum map construction,which is crucial in cognitive radio(CR)system,visualizes the invisible space of the electromagnetic spectrum for spectrum-resource management and allocation.Traditional reconstruction methods... Spectrum map construction,which is crucial in cognitive radio(CR)system,visualizes the invisible space of the electromagnetic spectrum for spectrum-resource management and allocation.Traditional reconstruction methods are generally for twodimensional(2D)spectrum map and driven by abundant sampling data.In this paper,we propose a data-model-knowledge-driven reconstruction scheme to construct the three-dimensional(3D)spectrum map under multi-radiation source scenarios.We firstly design a maximum and minimum path loss difference(MMPLD)clustering algorithm to detect the number of radiation sources in a 3D space.Then,we develop a joint location-power estimation method based on the heuristic population evolutionary optimization algorithm.Considering the variation of electromagnetic environment,we self-learn the path loss(PL)model based on the sampling data.Finally,the 3D spectrum is reconstructed according to the self-learned PL model and the extracted knowledge of radiation sources.Simulations show that the proposed 3D spectrum map reconstruction scheme not only has splendid adaptability to the environment,but also achieves high spectrum construction accuracy even when the sampling rate is very low. 展开更多
关键词 cognitive radio map reconstruction path loss model radiation source 3D spectrum map
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Octupole correlations of the K^(π)=5/2^(+)ground-state band in^(229)Th
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作者 Yuan-Yuan Wang Peng-Wei Zhao 《Chinese Physics B》 2026年第2期156-161,共6页
The octupole correlations of the K^(π)=5/2^(+)ground state and the rotational spectrum built on it in^(229)Th are studied using the microscopic relativistic density functional theory on a three-dimensional lattice sp... The octupole correlations of the K^(π)=5/2^(+)ground state and the rotational spectrum built on it in^(229)Th are studied using the microscopic relativistic density functional theory on a three-dimensional lattice space and the reflection-asymmetric triaxial particle rotor model.It is found that^(229)Th has a ground state with static axial octupole and quadrupole deformations.The occurrence of octupole correlations,driven by the octupole deformation,is analyzed through the evolution of single-particle levels around the Fermi surface.The experimental energy spectrum and the electromagnetic transition probabilities,including B(E2)and B(M1),are reasonably well reproduced. 展开更多
关键词 octupole correlation relativistic density functional theory reflection-asymmetric triaxial particle rotor model rotational spectrum
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Sum V2V Secrecy Rate Maximization for RIS-Assisted Vehicular Communication Systems with Spectrum Sharing
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作者 LI Zhiwei LI Demin CHEN Xuemin 《Journal of Donghua University(English Edition)》 2026年第1期68-79,共12页
Vehicular communication systems rely on secure vehicle-to-vehicle(V2V)communications for safety-critical information exchange.However,the presence of eavesdropping vehicles poses a significant challenge.This paper inv... Vehicular communication systems rely on secure vehicle-to-vehicle(V2V)communications for safety-critical information exchange.However,the presence of eavesdropping vehicles poses a significant challenge.This paper investigates the security of V2V communications in reconfigurable intelligent surface(RIS)-assisted vehicular communication systems with spectrum sharing.It proposes a three-stage alternating optimization(TSAO)algorithm to address the complex problem of multiple eavesdropped V2V links that reuse the spectrum already occupied by vehicle-toinfrastructure(V2I)links.To solve the mixed-integer and non-convex optimization problem due to coupled variables and complex constraints,the algorithm decomposes the original problem into three easily solvable sub-problems:RIS reflection coefficient optimization,vehicle transmission power optimization,and spectrum sharing optimization.First,the RIS reflection coefficients are optimized by using the penalty convex-concave procedure(CCP)method.Second,the optimal power points are determined in the power optimization sub-problem.Finally,the spectrum sharing optimization sub-problem is constructed as a weighted bipartite graph matching problem and solved by using the optimal matching algorithm.The TSAO algorithm not only maximizes the sum V2V secrecy rate but also ensures the quality-of-service(QoS)requirements of the V2I links.Simulation results validate the superiority of the proposed algorithm and highlight the improvement in the sum V2V secrecy rate achieved by utilizing RIS technology in vehicular communication systems with spectrum sharing. 展开更多
关键词 vehicle-to-vehicle(V2V)communication eavesdropping vehicle reconfigurable intelligent surface(RIS) secrecy rate spectrum sharing
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Effect of intense radiation on the X-ray emission spectrum of non-LTE plasmas
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作者 Chengwu Huang Tuo Zhu +12 位作者 Yuxue Zhang Tianming Song Yang Zhao Jiyan Zhang Zhiyu Zhang Gang Xiong Bo Qing Yan Zhao Liling Li Minxi Wei Zeqing Wu Jun Yan Jiamin Yang 《Matter and Radiation at Extremes》 2026年第2期28-33,共6页
Low-density non–local-thermodynamic-equilibrium plasmas in intense radiation fields occur widely in inertial confinement fusion and astrophysics. Understanding the X-ray spectrum and the atomic kinetics of such plasm... Low-density non–local-thermodynamic-equilibrium plasmas in intense radiation fields occur widely in inertial confinement fusion and astrophysics. Understanding the X-ray spectrum and the atomic kinetics of such plasmas is therefore of great importance. However, the creation of uniform-density nonequilibrium plasmas in intense radiation fields in the laboratory and the measurement of their spectra with high resolution are challenging tasks. Here, we present a new method to produce such a uniform aluminum plasma and explore photon-induced kinetics and relevant atomic physics by measuring its spectrum. It is observed that in the presence of an external radiation field, the satellites q, r and a–d of the He-α resonance line are greatly enhanced compared with the satellites j, k, l. Analysis of atomic kinetics reveals that this effect of intense radiation is due to competition between the photoexcitation and autoionization processes. With this effect taken into account,simulated spectra are able to reproduce the measured spectra quite well. 展开更多
关键词 inertial confinement fusion aluminum plasma measurement their spectra non lte plasmas x ray emission spectrum atomic kinetics intense radiation intense radiation fields
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Dynamic characterization of pathological and functional deterioration in a mouse model of optic neuritis related to neuromyelitis optica spectrum disorder
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作者 Xiayin Yang Shi-Qi Yao +1 位作者 Henry Ho-Lung Chan Shaoying Tan 《Neural Regeneration Research》 2026年第8期3870-3880,共11页
Neuromyelitis optica spectrum disorder-related optic neuritis involves various cellular responses to inflammation and degeneration.In most patients,the primary mechanism underlying neuromyelitis optica spectrum disord... Neuromyelitis optica spectrum disorder-related optic neuritis involves various cellular responses to inflammation and degeneration.In most patients,the primary mechanism underlying neuromyelitis optica spectrum disorder-related optic neuritis is the interaction of aquaporin-4 antibodies with the aquaporin-4 protein present on astrocytes within posterior optic nerve.This binding subsequently initiates a cascade of events leading to secondary demyelination of the optic nerve,ultimately culminating in optic nerve degeneration.Earlier studies on this disorder primarily used systemic-induced animal models,which often require prior activation of a systemic immune response.This can result in primary demyelination of the optic nerve,complicating the interpretation of experimental results.Such methodologies hinder the ability to isolate immune responses triggered by specific antibodies.Additionally,the lack of a detailed profile of disease progression over time limits our capacity to identify potential intervention windows.Therefore,constructing a targeted optic neuritis animal model induced by specific antibodies and elucidate the disease progression arecrucial for exploring the mechanisms underlying neuromyelitis optica spectrum disorder-related optic neuritis.In this study,specific antibodies against aquaporin-4 were precisely injected into the retrobulbar optic nerve of mice to induce a targeted inflammatory response in the posterior optic nerve,resulting in a more representative mouse model of neuromyelitis optica spectrum disorder-related optic neuritis than current models.The progression of the disease was then dynamically observed from both histological and functional perspectives over the course of 1 month following the induction of inflammation.By the first week,astrocytes were damaged,as evidenced by the loss of aquaporin-4 and glial fibrillary acidic protein,the activation of microglia,and the upregulation of microglia-related cytokines,including tumor necrosis factor,interleukin-6,interleukin-1β,C-X-C motif ligand 10,and brain-derived neurotrophic factor.Starting from the second week,there were signs of optic nerve demyelination and significant damage to axonal fibers and retinal ganglion cell bodies.Visual-evoked potentials and dark adaptation threshold responses in electroretinogram both indicated dysfunction in the visual pathway and retina,while optical coherence tomography revealed thinning of the retinal nerve fiber layer in live mice.In summary,in this study we conducted a dynamic exploration of the occurrence and progression of neuromyelitis optica spectrum disorder-related optic neuritis triggered by specific antibodies.Our results show pathological changes at various stages and correlate histological and molecular alterations with in vivo structural and functional deterioration.The findings from this study lay an important foundation for further research on neuromyelitis optica spectrum disorder-related optic neuritis. 展开更多
关键词 animal model aquaporin-4 immunoglobulin G dynamic profile ELECTRORETINOGRAM functional deterioration in vivo retinal structural scan neuromyelitis optica spectrum disorder-related optic neuritis optic neuritis pathology visual-evoked potential
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Explainable Ensemble Learning Framework for Early Detection of Autism Spectrum Disorder:Enhancing Trust,Interpretability and Reliability in AI-Driven Healthcare
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作者 Menwa Alshammeri Noshina Tariq +2 位作者 NZ Jhanji Mamoona Humayun Muhammad Attique Khan 《Computer Modeling in Engineering & Sciences》 2026年第1期1233-1265,共33页
Artificial Intelligence(AI)is changing healthcare by helping with diagnosis.However,for doctors to trust AI tools,they need to be both accurate and easy to understand.In this study,we created a new machine learning sy... Artificial Intelligence(AI)is changing healthcare by helping with diagnosis.However,for doctors to trust AI tools,they need to be both accurate and easy to understand.In this study,we created a new machine learning system for the early detection of Autism Spectrum Disorder(ASD)in children.Our main goal was to build a model that is not only good at predicting ASD but also clear in its reasoning.For this,we combined several different models,including Random Forest,XGBoost,and Neural Networks,into a single,more powerful framework.We used two different types of datasets:(i)a standard behavioral dataset and(ii)a more complex multimodal dataset with images,audio,and physiological information.The datasets were carefully preprocessed for missing values,redundant features,and dataset imbalance to ensure fair learning.The results outperformed the state-of-the-art with a Regularized Neural Network,achieving 97.6%accuracy on behavioral data.Whereas,on the multimodal data,the accuracy is 98.2%.Other models also did well with accuracies consistently above 96%.We also used SHAP and LIME on a behavioral dataset for models’explainability. 展开更多
关键词 Autism spectrum disorder(ASD) artificial intelligence in healthcare explainable AI(XAI) ensemble learning machine learning early diagnosis model interpretability SHAP LIME predictive analytics ethical AI healthcare trustworthiness
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粉被虫草液体静置发酵产N^(6)-(2-羟乙基)腺苷培养基优化 被引量:1
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作者 廖兴刚 孟泽彬 +1 位作者 贺凤姣 文庭池 《分子植物育种》 北大核心 2025年第9期3046-3051,共6页
为了获得粉被虫草中高产N^(6)-(2-羟乙基)腺苷(HEA)的培养基,采用液体静置发酵的方式,以菌丝体HEA的产量为指标,HPLC法检测含量,采用单因素试验法研究培养基中8种碳源、8种氮源和8种无机盐对粉被虫草产HEA的影响;用正交试验筛选最佳培... 为了获得粉被虫草中高产N^(6)-(2-羟乙基)腺苷(HEA)的培养基,采用液体静置发酵的方式,以菌丝体HEA的产量为指标,HPLC法检测含量,采用单因素试验法研究培养基中8种碳源、8种氮源和8种无机盐对粉被虫草产HEA的影响;用正交试验筛选最佳培养基组合。结果显示,蛋白胨、果糖和ZnSO4分别是粉被虫草产HEA的最适氮源、最适碳源和最适无机盐;其最佳培养基组合为蛋白胨15 g/L、果糖40 g/L、硫酸锌0.5 g/L,此时的HEA产量为(92.311±2.209)mg/L,是目前报道粉被虫草中的最高产量。本研究将为粉被虫草规模生产HEA提供试验参考。 展开更多
关键词 粉被虫草 N^(6)-(2-羟乙基)腺苷 液体静置发酵 培养基
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具有n^(+)埋层和L型场板的Si/SiC异质结沟槽LDMOS器件 被引量:1
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作者 康怡 刘东 +2 位作者 卢山 鲁啸龙 胡夏融 《半导体技术》 北大核心 2025年第2期134-140,共7页
Si/4H-SiC异质结构能够同时结合Si材料的成熟工艺和SiC材料的宽禁带特性,在功率器件设计中具有巨大潜力。提出了一种具有n+埋层和L型场板的Si/SiC异质结沟槽横向双扩散金属氧化物半导体(LDMOS)器件。位于Si/SiC异质结界面SiC侧的重掺杂n... Si/4H-SiC异质结构能够同时结合Si材料的成熟工艺和SiC材料的宽禁带特性,在功率器件设计中具有巨大潜力。提出了一种具有n+埋层和L型场板的Si/SiC异质结沟槽横向双扩散金属氧化物半导体(LDMOS)器件。位于Si/SiC异质结界面SiC侧的重掺杂n+埋层能够有效降低界面势垒宽度,增强电子隧穿效应,降低界面电阻,进一步降低比导通电阻。位于厚氧化层角落并与漏极相连的L型场板通过在SiC漂移区和厚氧化层之间产生高电场,重塑器件横向和纵向电场强度分布,将击穿点从表面转移至体内,提高击穿电压。仿真结果表明,与传统SiC LDMOS器件相比,该器件的品质因数从109.29 MW/cm^(2)提升至159.92 MW/cm^(2),提高了46.36%,进一步改善了LDMOS器件导通电阻和击穿电压之间的折中关系,器件性能得到优化。 展开更多
关键词 横向双扩散金属氧化物半导体(LDMOS) Si/4H-SiC异质结 n^(+)埋层 L型场板 功率品质因数
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