The mixed forests of the upper Rio Negro at the northern of the Amazon basin grow in oxisol soils that are extremely infertile. These areas exhibit deficiencies in several macro-nutrients, and may also be characterize...The mixed forests of the upper Rio Negro at the northern of the Amazon basin grow in oxisol soils that are extremely infertile. These areas exhibit deficiencies in several macro-nutrients, and may also be characterized by the shortage or toxic excess of some micronutrients. The overall goal of this research is to collect more comprehensive information regarding the micronutrient composition of the upper Rio Negro forests as well as discern the relationship between leaf micro- and macro-nutrients that may contribute to the homeostasis and balance of the ionome. Firstly, the nutrient composition within the oxisol soil and leaf tissues of two top canopy tree species from the mixed forests was determined. We then analyzed the relationship between leaf micronutrient composition with N and P levels of the two species and that of species inhabiting the Amazon caatinga. Extractable soil Zn, B, Mn and Cu were very low in the mixed forest. In contrast, Fe and Al levels were potentially toxic. The analysis of leaf N/P ratios revealed for the first time the co-limitation of N and P in the mixed forest. This contrasts with species from the adjacent Amazon caatinga toposequence that are characterized by strong N limitation. All micronutrients within leaves of species inhabiting the mixed forest were also found to have low concentrations. Moreover, Fe and Al were detected at concentrations well below those reported for accumulator species. This suggested that leaf ion homeostasis was maintained under potentially toxic soil Fe and Al conditions. Leaf micronutrient (Fe, Zn and B) contents mirrored that of leaf N and P contents, and comparable Fe/N, Fe/P, Zn/N, Zn/P, B/N as well as B/P ratios were found across species and forest types. Therefore, forest species exhibited the capability to maintain leaf nutrient balances under soil conditions with deficient or toxic levels of micronutrients.展开更多
The concentrations of the foliar and surface soil nutrients and the variation with species and stand age were studied inPinus spp. plantations in Zhanggutai area, northeast China. The results showed that the total N, ...The concentrations of the foliar and surface soil nutrients and the variation with species and stand age were studied inPinus spp. plantations in Zhanggutai area, northeast China. The results showed that the total N, total P and C: N ratio of the soil inP. sylvestris var.mongolica stands were significantly higher in comparison with those inP. tabulaeformis andP. densiflora stands. ForP. sylvestris var.mongolica, the foliar P concentration appeared to decrease with age, and the foliar N and K concentrations did not show a consistent change with age. As for the different tree species of the similar age, the foliar N and P concentrations were significantly different (p<0.05), being withP. sylvestris var.mongolica>P. densiflora>P. tabulaeformis. The foliar N: P ratio ofP. densiflora significantly was higher thanP. sylvestris var.mongolica andP. tabulaeformis, while the foliar K was no obvious difference between the three tree species. There were significant correlation (p<0.05) between soil total N and P, soil organic matter and total P, foliar N and P, but it did not show significant correlations between soil and foliar nutrient concentrations, which might attribute to the excessive litter raking, overgrazing and low soil moisture in this area. Based on the foliar N: P ratio, we introduced a combination threshold index of N: P ratio with their absolute foliar nutrient concentrations to determine the possible limiting nutrient. According to the critical N: P ratio and their absolute foliar N, P concentrations, theP. sylvestris var.mongolica stands showed a decreased N limitation degree with age, theP. densiflora stands showed unlimited by N and P in the whole, and theP. tabulaeformis stands showed co-limited by N and P. No significant difference in soil nutrient concentrations of the surface soils was found between 45, 29, 20-yr-oldPinus sylvestris var.mongolica plantation stands. Keywords coniferous trees - foliar nutrient concentration - limiting nutrients - N - P ratio - Zhanggutai sandy land CLC number S718.55 Document code A Article ID 1007-662X(2004)01-0011-08 Foundation item: This research was supported by Key Knowledge Innovation Project (KZCX3-SW-418) of Chinese Academy of Sciences.Biography: CHEN Guang-sheng (1978-), male, master candidate in Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P. R. ChinaResponsible editor: Song Funan展开更多
Combined inoculation with dark septate endophytes(DSEs)and arbuscular mycorrhizal fungi(AMF)has been shown to promote plant growth,yet the underlying plant-fungus interaction mechanisms remain unclear.To elucidate the...Combined inoculation with dark septate endophytes(DSEs)and arbuscular mycorrhizal fungi(AMF)has been shown to promote plant growth,yet the underlying plant-fungus interaction mechanisms remain unclear.To elucidate the nature of this symbiosis,it is crucial to explore carbon(C)transport from plants to fungi and nutrient exchange between them.In this study,a pot experiment was conducted with two phosphorus(P)fertilization levels(low and normal)and four fungal inoculation treatments(no inoculation,single inoculation of AMF and DSE,and co-inoculation of AMF and DSE).The^(13)C isotope pulse labeling method was employed to quantify the plant photosynthetic C transfer from plants to different fungi,shedding light on the mechanisms of nutrient exchange between plants and fungi.Soil and mycelium δ^(13)C,soil C/N ratio,and soil C/P ratio were higher at the low P level than at the normal P level.However,soil microbial biomass C/P ratio was lower at the low P level,suggesting that the low P level was beneficial to soil C fixation and soil fungal P mineralization and transport.At the low P level,the P reward to plants from AMF and DSE increased significantly when the plants transferred the same amount of C to the fungi,and the two fungi synergistically promoted plant nutrient uptake and growth.At the normal P level,the root P content was significantly higher in the AMF-inoculated plants than in the DSE-inoculated plants,indicating that AMF contributed more than DSE to plant P uptake with the same amount of C received.Moreover,plants preferentially allocated more C to AMF.These findings indicate the presence of a source-sink balance between plant C allocation and fungal P contribution.Overall,AMF and DSE conferred a higher reward to plants at the low P level through functional synergistic strategies.展开更多
Nitrogen(N)and phosphorus(P)are two essential nutrients that determine plant growth and many nutrient cycling processes.Increasing N and P deposition is an important driver of ecosystem changes.However,in contrast to ...Nitrogen(N)and phosphorus(P)are two essential nutrients that determine plant growth and many nutrient cycling processes.Increasing N and P deposition is an important driver of ecosystem changes.However,in contrast to numerous studies about the impacts of nutrient addition on forests and temperate grasslands,how plant foliar stoichiometry and nutrient resorption respond to N and P addition in alpine grasslands is poorly understood.Therefore,we conducted an N and P addition experiment(involving control,N addition,P addition,and N+P addition)in an alpine grassland on Kunlun Mountains(Xinjiang Uygur Autonomous Region,China)in 2016 and 2017 to investigate the changes in leaf nutrient concentrations(i.e.,leaf N,Leaf P,and leaf N:P ratio)and nutrient resorption efficiency of Seriphidium rhodanthum and Stipa capillata,which are dominant species in this grassland.Results showed that N addition has significant effects on soil inorganic N(NO_(3)^(-)-N and NH_(4)^(+)-N)and leaf N of both species in the study periods.Compared with green leaves,leaf nutrient concentrations and nutrient resorption efficiency in senesced leaves of S.rhodanthum was more sensitive to N addition,whereas N addition influenced leaf N and leaf N:P ratio in green and senesced leaves of S.capillata.N addition did not influence N resorption efficiency of the two species.P addition and N+P addition significantly improved leaf P and had a negative effect on P resorption efficiency of the two species in the study period.These influences on plants can be explained by increasing P availability.The present results illustrated that the two species are more sensitive to P addition than N addition,which implies that P is the major limiting factor in the studied alpine grassland ecosystem.In addition,an interactive effect of N+P addition was only discernable with respect to soil availability,but did not affect plants.Therefore,exploring how nutrient characteristics and resorption response to N and P addition in the alpine grassland is important to understand nutrient use strategy of plants in terrestrial ecosystems.展开更多
Microbial growth in soil is mostly limited by lack of carbon (C). However, adding fresh, C-rich litter can induce nitrogen (N) limitation. We studied the effect of alleviating C and N limitation in high-pH (> 8) so...Microbial growth in soil is mostly limited by lack of carbon (C). However, adding fresh, C-rich litter can induce nitrogen (N) limitation. We studied the effect of alleviating C and N limitation in high-pH (> 8) soils, soils expected to favor bacterial over fungal growth. Nitrogen limitation was induced by incubating soils amended with C-rich substrate (starch or straw) for 4 weeks. Limiting nutrients and the effects of alleviating limitation were then studied by adding C (as glucose) or N (as NH_4NO_3) and measuring microbial growth and respiration after 4 d. In non-amended, C-limited soils, adding C but not N increased both microbial respiration and bacterial growth. In N-limited, substrate-amended soils, adding C increased respiration, whereas adding N increased both microbial respiration and growth. Inducing N limitation by amending with straw was most easily detected in increased fungal growth after the addition of N, whereas with starch, only bacterial growth responded to alleviating N limitation. Compared to earlier results using a low-pH soil, the effect of substrate used to induce N limitation was more important than pH for inducing bacterial or fungal growth after alleviating N limitation. Furthermore, we found no evidence that alleviating N limitation resulted in decreased respiration concomitant with increased microbial growth in soil, suggesting no drastic changes in C use efficiency.展开更多
Environments with reciprocal patchiness of resources, in which the availability of two resources such as light and soil nutrients are patchily distributed in horizontal space and negatively correlated in each patch, a...Environments with reciprocal patchiness of resources, in which the availability of two resources such as light and soil nutrients are patchily distributed in horizontal space and negatively correlated in each patch, are common in many ecosystems. The strategies by which clonal plants adapt to this type of heterogeneous environment were examined in three stoloniferous herbs,Potentilla reptans L. var. sericophylla Franch., P. anserina L. and Halerpestes ruthenica (Jacq.) Qvcz., commonly inhabiting forest understories, grasslands and low saline meadows, respectively. As pairs of connected ramets were subjected to reciprocal patchiness of light and nutrients, stolon connection between the two ramets significantly enhanced biomass of both ramet growing in low light intensity but high soil nutrient condition (LH ramet) and ramet growing in high light intensity but low soil nutrient condition (HL ramet) as well as whole ramet pairs (consisting of LH ramets and HL ramets). Additionally, stolon connection greatly increased root/shoot ratio of LH ramet while significantly decreased that of HL ramet. The results indicate that a reciprocal transportation of resources between interconnected ramets and a functional specialization of ramets in uptake of abundant resources occurred. By resource sharing and functional specialization, clonal plants can efficiently acquire locally abundant resources and buffer the stress caused by reciprocal patchiness of resources.展开更多
In order to examine the causes of degradation of Pinus sylvestris var.mongolica plantations on sandy land,the foliar concentrations of N,P,K and C were analyzed and compared between the field grown P.sylvestris var.mo...In order to examine the causes of degradation of Pinus sylvestris var.mongolica plantations on sandy land,the foliar concentrations of N,P,K and C were analyzed and compared between the field grown P.sylvestris var.mongolica trees from two provenances(natural forests and plantations).The results indicated that natural tree needles had lower N,P and C concentrations,and higher K concentrations than those of plantation tree needles.For plantation tree needles,ratios of N:P,P.K and N:K increased with tree age before 45 years old;but they were not clear for the natural tree needles.Compared with the conclusions reported on Pinus spp.,we found that the foliar N and P concentrations were in the optimal range for both natural and plantation tree needles.This result suggested that N or P might not be the absolute limit factors in plant nutrient for P sylvestris var.mongolica on sandy land.However,foliar K concentrations in both natural and plantation tree needles were much lower than those reported on Pinus spp.(〉4.80 g kg-1).The N:P ratio of natural needles was in the adequate ranges,but N:P ratio of plantation needles was out of the adequate ranges.These results indicated that there was a better balanced nutrition status in the natural forest than in the plantations.If only considering the foliar nutrient concentrations of P sylvestris var.mongolica from different provenances,it might be concluded that the degradation phenomenon of P.sylvestris var.mongolica plantations was not induced by nutrition deficiency of absolute nutrients of N and P,but might be induced by other mineral nutrients or by the effectiveness of N and P nutrients.The unbalanced nutrition status and relatively quick decomposition of needles in the plantations might also contribute to the degradation.展开更多
In the present paper, some novel opportunities for the development of high-efficient Si and III-V-based solar cells are considered: energy-saving environment friendly low-temperature technology of forming p-n junction...In the present paper, some novel opportunities for the development of high-efficient Si and III-V-based solar cells are considered: energy-saving environment friendly low-temperature technology of forming p-n junctions in Si (1), elaboration of structurally perfect GaAs/Ge/Si epitaxial substrates (2) and application of protective antireflecting coatings based on cubic zirconia (3). As a result: 1) New technique of forming p-n junctions in silicon has been elaborated. The technique provided easy and comparatively cheap process of production of semiconductor devices such as solar cells. The essence of the technique under the study is comprised in formation p-n junctions in silicon by a change of conductivity in the bulk of the sample occurring as a result of redistribution of the impurities, which already exists in the sample before its processing by ions. It differs from the techniques of diffusion and ion doping where change of conductivity and formation of p-n junction in the sample occur as a result of introduction of atoms of the other dopants from the outside;2) The conditions for synthesis of GaAs/Ge/Si epitaxial substrates with a thin (200 nm) Ge buffer layer featured with (1 - 2) × 105 cm-2 density of the threading dislocation in the GaAs layer. Ge buffer was obtained by chemical vapor deposition with a hot wire and GaAs layer of 1 μm thick was grown by the metal organic chemical vapor deposition. Root mean square surface roughness of GaAs layers of the less than 1 nm and good photoluminescence properties along with their high uniformity were obtained;3) The conditions ensuring the synthesis of uniform functional (buffer, insulating and protective) fianite layers on Si and GaAs substrates by means of magnetron and electron-beam sputtering have been determined. Fianite films have been shown to be suitable for the use as an ideal anti-reflecting material with high protective and anticorrosive properties.展开更多
Seven-day-old maize (Zea mays) plants were grown hydroponically for ten days in deprived nutrient solutions against the corresponding control grown under full nutrition;the effects of S-, N- or P-deprivation on lamina...Seven-day-old maize (Zea mays) plants were grown hydroponically for ten days in deprived nutrient solutions against the corresponding control grown under full nutrition;the effects of S-, N- or P-deprivation on laminas’ mean stomatal conductance (gs), transpiration rate (E) and photosynthetic rate (A) were monitored, along with the impact on the laminas’ total dry mass (DM), water amount (W), length and surface area (Sa). Furthermore, a time series analysis of each parameter’s response ratios (Rr), i.e. the treatment’s value divided by the corresponding control’s one, was performed. Under S-deprivation, the Rr of laminas’ mean gs, E, and A presented oscillations within a ±15% fluctuation zone, notably the “control” zone, whilst those of laminas’ total DM, water amount, surface area, and length included oscillation during the first days and deviation later on, presenting deviation during d10. Under the N-deprivation conditions all Rr time courses except the A one, included early deviations from the control zone without recovering. The deviation from the control zone appeared at d4. Under P-deprivation, all Rr time courses represented oscillations within the control zone. P-deprivation’s patterns resembled those of S-deprivation. Compared to the one of the S-deprivation, the P-one’s oscillations took place within a broader zone. Linear relationships among the various Rr patterns were found between gs-E, gs-A, E-A, DM-W and DM-Sa. In conclusion, the impact of P-deprivation appeared in an early stage and included an alleviation action, the one of N-deprivation appeared early with no alleviation action, whilst that of S-deprivation appeared later, being rather weaker when compared to the impact of the P-deprivation’s impact.展开更多
Attitudes regarding traditional energy sources have shifted toward renewable resources. Specifically, short-rotation woody crop supply systems have become more prevalent for biomass and biofuel production. However, a ...Attitudes regarding traditional energy sources have shifted toward renewable resources. Specifically, short-rotation woody crop supply systems have become more prevalent for biomass and biofuel production. However, a number of factors such as environmental and inherent resource availability can limit tree production. Given the intensified demand for wood biomass production, forest and plantation management practices are focusing on increasing productivity. Fertilizer application, while generally one of the least expensive silvicultural tools, can become costly if application rates exceed nutrient uptake or demand of the trees especially if it does not result in additional biomass production. We investigated the effect of water and varying levels of nitrogen application (56, 112, and 224 kg·N·haǃ·yrǃ) on nutrient content, resorption efficiency and proficiency, N:P and the relationship with ANPP, as well as leaf- and canopy-level nutrient use efficiency of nitrogen, phosphorus, and potassium for Populus deltoides, Quercus pagoda, and Platanus occidentalis. P. deltoides and P. occidentalis reached their maximum nitrogen budget with the application of water suggesting old agricultural fields may have sufficient nutrient levels to sustain short-rotation woody crops negating the application of additional nitrogen for these two species. Additionally, for P. deltoides and Q. pagoda application of nitrogen appeared to increase the uptake of phosphorus however, resorption efficiency for these two species were more similar to studies conducted on nutrient poor sites. Nutrient resorption proficiency for all three nutrients and all three species were at levels below the highest rates of nitrogen application. These findings suggest maximum biomass production may not necessarily be tied to maximum nutrient application.展开更多
The coastal upwelling has profound influence on the surrounding ecosystem by supplying the nutrient-replete water to the euphotic zone.Nutrient biogeochemistry was investigated in coastal waters of the eastern Hainan ...The coastal upwelling has profound influence on the surrounding ecosystem by supplying the nutrient-replete water to the euphotic zone.Nutrient biogeochemistry was investigated in coastal waters of the eastern Hainan Island in summer 2015 and autumn 2016.From perspectives of nutrient dynamics and physical transport,the nutrient fluxes entered the upper 50 m water depth(between the mixed layer and the euphotic zone)arisen from the upwelling were estimated to be 2.5-5.4 mmol/(m^(2)·d),0.15-0.28 mmol/(m^(2)·d),and 2.2-7.2 mmol/(m^(2)·d)for dissolved inorganic nitrogen(DIN),phosphate(DIP),and dissolved silicate(DSi),respectively,which were around 6-to 12-fold those in the background area.The upwelled nutrients supported an additional plankton growth of(14.70±8.95)mg/m^(2)for chlorophyll a(Chl a).The distributions of nitrateδ^(15)N andδ^(18)O above the 300 m water depth(top of the North Pacific Intermediate Water)were different among the upwelling area,background area in summer,and the stations in autumn,and the difference of environmental and biogeochemical conditions between seasons should be the reason.The higher DIN/DIP concentration ratio,nitrate concentration anomaly,and lower nitrate isotope anomaly(Δ(15,18))in the upper ocean in summer than in autumn indicated the stronger nitrogen fixation and atmospheric deposition,and the following fixed nitrogen regeneration in summer.The higher values of Chl a and nitrateδ^(15)N andδ^(18)O within the euphotic zone in autumn than the background area in summer suggested the stronger nitrate assimilation in autumn.The differences in relatively strength of the assimilation,nitrogen fixation and atmospheric deposition,and the following remineralization and nitrification between the two seasons made the higherδ^(18)O:δ^(15)N and larger difference of enzymatic isotope fractionation factors^(15)εand^(18)εfor nitrate assimilation in summer than in autumn above the North Pacific Tropical Water.展开更多
This paper investigates the current-voltage characteristics (CVC) strain of p-n-junction in a strong microwave (MW) field and shows that the deformation increases the current generated in the p-n-junction. We analyze ...This paper investigates the current-voltage characteristics (CVC) strain of p-n-junction in a strong microwave (MW) field and shows that the deformation increases the current generated in the p-n-junction. We analyze the current-voltage characteristics of p-n-junction in which three-dimensional space (I,U,e) gives more complete information than the two-dimensional.展开更多
Botanical, soil chemistry and soil microbiology variables were tested as predictors of in situ soil respiration rate in the various terrestrial habitats on sub-Antarctic Marion Island (47oS, 38oE). Inorganic P and tot...Botanical, soil chemistry and soil microbiology variables were tested as predictors of in situ soil respiration rate in the various terrestrial habitats on sub-Antarctic Marion Island (47oS, 38oE). Inorganic P and total N concentration were the best predictors amongst the chemistry variables and bacteria plate count the best of the microbiology variables. However, while these chemistry and microbiology variables could accurately predict soil respiration rate for particular habitats, they proved inadequate predictors across the whole range of habitats. The best suite of predictors comprised only botanical variables (relative covers of five plant guilds) and accounted for 94% of the total across-habitat variation in soil respiration rate. Mean field soil respiration rates (2.1 - 15.5 mmol CO2 m-2 h-1) for habitats not influenced by seabirds or seals are similar to rates in comparable Northern Hemisphere tundra habitats. Seabird and seal manuring enhances soil respiration rates to values (up to 27.6 mmol CO2 m-2 h-1) higher than found at any tundra site. Glucose, N, P or N plus P were added to three habitats with contrasting soil types;a fellfield with mineral, nutrient-poor soil, a mire with organic, nutrient-poor soil and a shore-zone herbfield heavily manured by penguins and with organic, nutrient-rich soil. Glucose addition stimulated soil respiration in the fellfield and mire (especially the former) but not in the coastal herbfield soil. N and P, alone or together, did not stimulate respiration at any of the habitats, but adding glucose to fellfield soils that had previously been fortified with P or NP caused a similar increase in respiration rate, which was greater than the increase when adding glucose to soils fortified only with N. This suggests that fellfield soil respiration is limited by P rather than N, and that there is no synergism between the two nutrients. For the mire and coastal herbfield, adding glucose to soils previously fortified with N, P or NP did not enhance rates more than adding glucose to soils that had received no nutrient pre-treatment.展开更多
Field and glasshouse experiments were conducted to study the effect of B. japonicum inoculation and phosphorus supplementation on macronutrient uptake by soybean. The treatments consisted of B. japonicum inoculation (...Field and glasshouse experiments were conducted to study the effect of B. japonicum inoculation and phosphorus supplementation on macronutrient uptake by soybean. The treatments consisted of B. japonicum inoculation (with & without), phosphorus supplementation at the levels of 0, 20, 40 and 80 kg P·ha-1. Both treatments were replicated four times in a split plot design. The macronutrients considered were N, P, K, Ca and Mg. Results showed that inoculation with B. japonicum significantly contribute to the uptake of N, P, K, Ca and Mg in roots, shoots, pods and the whole soybean plant. Likewise, phosphorus supplementation significantly enhanced the uptake of N, P, K, Ca and Mg in roots, shoots, pods and the whole plant. The use of effective strains of rhizobia and P supplementation was an effective way of enhancing the growth of soybean, eventually the uptake of macronutrients in plant organs.展开更多
The sluggish kinetics of the oxygen reduction reaction(ORR)and high over potential of oxygen evolution reaction(OER)are big challenges in the development of high-performance zinc-air batteries(ZABs)and fuel cells.In t...The sluggish kinetics of the oxygen reduction reaction(ORR)and high over potential of oxygen evolution reaction(OER)are big challenges in the development of high-performance zinc-air batteries(ZABs)and fuel cells.In this work,we report a rational design and a simple fabrication strategy of a photo-enhanced Co single-atom catalyst(SAC)comprising g-C3N4 coupled with cobalt-nitrogen-doped hierarchical mesoporous carbon(Co-N/MPC),forming a staggered p-n heterojunction that effectively improves charge separation and enhances electrocatalytic activity.The incorporation of Co SACs and g-C3N4 synergistically optimizes the photogenerated electron-hole pair separation,significantly boosting the intrinsic ORR-OER duplex activity.Under illumination,g-C_(3)N_(4)@Co-N/MPC exhibits an outstanding ORR half-wave potential(E1/2)of 0.841 V(vs.RHE)in 0.1 mol L^(–1)KOH and a low OER overpotential of 497.4 mV(vs.RHE)at 10 mA cm^(–2)in 1 mol L^(–1)KOH.Notably,the catalyst achieves an exceptional peak power density of 850.7 mW cm^(–2)in ZABs and of 411 mW cm^(–2)even in H_(2)-air fuel cell.In addition,g-C_(3)N_(4)@Co-N/MPC-based ZABs also show remarkable cycling stability exceeding 250 h.The advanced photo-induced charge separation at the p-n heterojunction facilitates faster electron transfer kinetics,and the mass transport owing to hierarchical mesoporous structure of Co-N-C,thereby reducing the overpotential and enhancing the overall energy conversion efficiency.This work provides a new perspective on designing next-generation of single-atom dispersed oxygen reaction catalysts,paving the way for high-performance photo-enhanced energy storage and conversion systems.展开更多
Nitrogen(N)deficiency is a critical factor limiting natural regeneration in coastal shelterbelt forests,but the influence of different N forms on seedling establishment under varying light conditions remains poorly un...Nitrogen(N)deficiency is a critical factor limiting natural regeneration in coastal shelterbelt forests,but the influence of different N forms on seedling establishment under varying light conditions remains poorly understood.This study investigated the effects of N forms and N concentrations on Ligustrum compactum seedlings under simulated canopy gap conditions using a three-factor design:N form(NO_(3)^(-)-N,NH_(4)^(+)-N,mixed N),N concentration(30 and 60 kg ha^(-1)a^(-1)),and light intensity(30%,60%,and 90%full sunlight).Results showed that N addition significantly promoted seedling growth,net photosynthesis rate,and water use efficiency;however,the effects varied among N forms and concentrations.Overall,NO_(3)^(-)-N or mixed N were more favored by L.compactum seedlings;however,the N preference was altered by light intensity and N concentration.For instance,L.compactum showed greater NO_(3)^(-)-N or mixed N preference under low and medium light intensities,while displaying more NH_(4)^(+)-N preference under high light intensity.N concentration also affected the growth and N preference of L.compactum seedlings,but the variance explained by N concentration was lower than that of light intensity.Leaf C,N,P stoichiometry exhibited stronger correlations with seedling's morphological trait plasticity than those of leaf gas exchange,and further analysis demonstrated that leaf C:P and N:P were the top two critical factors affecting seedling growth,indicating that the coordination and balance among C,N,P elements were more important in explaining the seedling growth under N addition.Therefore,our results clarified that the N preference of L.compactum seedlings could be altered by light intensity and revealed that leaf C,N,P ratios were stronger predictors than leaf gas exchange parameters for explaining the N effects on seedling performance.These findings demonstrated the mechanisms of light-N interactions affecting seedling performance,providing practical guidance for optimizing N fertilization and improving natural regeneration in canopy gaps of degraded coastal shelterbelt forests.展开更多
The effect of different sources and levels of N on dry matter production, nutrient uptake and ionic balance ofLarlix gmelini was studied. The results showed that the growth of the plants fertilized with ammonium was n...The effect of different sources and levels of N on dry matter production, nutrient uptake and ionic balance ofLarlix gmelini was studied. The results showed that the growth of the plants fertilized with ammonium was not as good as the control treatment. The growth of the plants fertilized with ammonium nitrate did not differ significantly from that in control or nitrate treatment, but was better than that in the ammonium treatment. Total cation concentrations in shoots varied little with N level in the ammonium and ammonium nitrate treatments, while those in the shoot increased with N level in the nitrate treatment. The treatments had little effect on the anion concentrations in the shoot. In the roots, the concentrations of both cations and anions changed little except for SO4 2? and Ca2+. There existed a higher carboxylate production in the plants fertilized with nitrate. The ratio between the production of carboxylate and the production of organic N Δ(C-A)/ΔNorg was constant with N supply in the plants receiving nitrate, but obviously declined with N supply for ammonium-fed plants. Δ (C-A)/ΔNorg values were intermediate between those of the nitrate and the ammonium-fed plants as for the mixed N source.展开更多
Increasing levels of pollution within water bodies can cause eutrophication and an associated rapid growth in and reproduction of phytoplankton. Although most frequently occurring in bodies of water such as lakes and ...Increasing levels of pollution within water bodies can cause eutrophication and an associated rapid growth in and reproduction of phytoplankton. Although most frequently occurring in bodies of water such as lakes and dams, in recent years an increasing number of river systems in China have suffered serious algal blooms. The community structure of phytoplankton may differ, however, dependent on the hydrodynamic conditions and nutrient levels within the water body. The field investigation results obtained from a stagnant river in Suzhou City and Taihu Lake, China, showed that in water with higher concentrations of nitrogen and phosphorus, Chlorophyta became the predominant species and in water with lower concentrations of nitrogen and phosphorus, Cyanobacteria became the predominant species. Growth experiments with competitive species, Microcystis aeruginosa Kutz and Scenedesmus quadricauda （Turp.）, were conducted at three different nutrient levels. The biomass of algae in pure and mixed cultures was measured under conditions of different N/P ratios at oligotrophic, eutrophic and hypertrophic nutrient levels. The results indicated that the most suitable state for the growth and reproduction of M. aeruginosa and S. quadricauda were eutrophic conditions in both pure and mixed cultures. Under competition, however, the lower medium nutrient levels favoured M. aeruginosa, while the higher medium nutrient levels better suited S. quadricauda. Under similar hydrodynamic conditions, the community structure of phytoplankton in the water body was determined by the dominant species in competition for nutrients.展开更多
文摘The mixed forests of the upper Rio Negro at the northern of the Amazon basin grow in oxisol soils that are extremely infertile. These areas exhibit deficiencies in several macro-nutrients, and may also be characterized by the shortage or toxic excess of some micronutrients. The overall goal of this research is to collect more comprehensive information regarding the micronutrient composition of the upper Rio Negro forests as well as discern the relationship between leaf micro- and macro-nutrients that may contribute to the homeostasis and balance of the ionome. Firstly, the nutrient composition within the oxisol soil and leaf tissues of two top canopy tree species from the mixed forests was determined. We then analyzed the relationship between leaf micronutrient composition with N and P levels of the two species and that of species inhabiting the Amazon caatinga. Extractable soil Zn, B, Mn and Cu were very low in the mixed forest. In contrast, Fe and Al levels were potentially toxic. The analysis of leaf N/P ratios revealed for the first time the co-limitation of N and P in the mixed forest. This contrasts with species from the adjacent Amazon caatinga toposequence that are characterized by strong N limitation. All micronutrients within leaves of species inhabiting the mixed forest were also found to have low concentrations. Moreover, Fe and Al were detected at concentrations well below those reported for accumulator species. This suggested that leaf ion homeostasis was maintained under potentially toxic soil Fe and Al conditions. Leaf micronutrient (Fe, Zn and B) contents mirrored that of leaf N and P contents, and comparable Fe/N, Fe/P, Zn/N, Zn/P, B/N as well as B/P ratios were found across species and forest types. Therefore, forest species exhibited the capability to maintain leaf nutrient balances under soil conditions with deficient or toxic levels of micronutrients.
基金Key Knowledge Innovation Project (KZCX3-SW-418) of Chinese Academy of Sciences.
文摘The concentrations of the foliar and surface soil nutrients and the variation with species and stand age were studied inPinus spp. plantations in Zhanggutai area, northeast China. The results showed that the total N, total P and C: N ratio of the soil inP. sylvestris var.mongolica stands were significantly higher in comparison with those inP. tabulaeformis andP. densiflora stands. ForP. sylvestris var.mongolica, the foliar P concentration appeared to decrease with age, and the foliar N and K concentrations did not show a consistent change with age. As for the different tree species of the similar age, the foliar N and P concentrations were significantly different (p<0.05), being withP. sylvestris var.mongolica>P. densiflora>P. tabulaeformis. The foliar N: P ratio ofP. densiflora significantly was higher thanP. sylvestris var.mongolica andP. tabulaeformis, while the foliar K was no obvious difference between the three tree species. There were significant correlation (p<0.05) between soil total N and P, soil organic matter and total P, foliar N and P, but it did not show significant correlations between soil and foliar nutrient concentrations, which might attribute to the excessive litter raking, overgrazing and low soil moisture in this area. Based on the foliar N: P ratio, we introduced a combination threshold index of N: P ratio with their absolute foliar nutrient concentrations to determine the possible limiting nutrient. According to the critical N: P ratio and their absolute foliar N, P concentrations, theP. sylvestris var.mongolica stands showed a decreased N limitation degree with age, theP. densiflora stands showed unlimited by N and P in the whole, and theP. tabulaeformis stands showed co-limited by N and P. No significant difference in soil nutrient concentrations of the surface soils was found between 45, 29, 20-yr-oldPinus sylvestris var.mongolica plantation stands. Keywords coniferous trees - foliar nutrient concentration - limiting nutrients - N - P ratio - Zhanggutai sandy land CLC number S718.55 Document code A Article ID 1007-662X(2004)01-0011-08 Foundation item: This research was supported by Key Knowledge Innovation Project (KZCX3-SW-418) of Chinese Academy of Sciences.Biography: CHEN Guang-sheng (1978-), male, master candidate in Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P. R. ChinaResponsible editor: Song Funan
基金supported by the National Key Research and Development Program of China(No.2022YFF 1303303)the National Natural Science Foundation of China(No.52394194).
文摘Combined inoculation with dark septate endophytes(DSEs)and arbuscular mycorrhizal fungi(AMF)has been shown to promote plant growth,yet the underlying plant-fungus interaction mechanisms remain unclear.To elucidate the nature of this symbiosis,it is crucial to explore carbon(C)transport from plants to fungi and nutrient exchange between them.In this study,a pot experiment was conducted with two phosphorus(P)fertilization levels(low and normal)and four fungal inoculation treatments(no inoculation,single inoculation of AMF and DSE,and co-inoculation of AMF and DSE).The^(13)C isotope pulse labeling method was employed to quantify the plant photosynthetic C transfer from plants to different fungi,shedding light on the mechanisms of nutrient exchange between plants and fungi.Soil and mycelium δ^(13)C,soil C/N ratio,and soil C/P ratio were higher at the low P level than at the normal P level.However,soil microbial biomass C/P ratio was lower at the low P level,suggesting that the low P level was beneficial to soil C fixation and soil fungal P mineralization and transport.At the low P level,the P reward to plants from AMF and DSE increased significantly when the plants transferred the same amount of C to the fungi,and the two fungi synergistically promoted plant nutrient uptake and growth.At the normal P level,the root P content was significantly higher in the AMF-inoculated plants than in the DSE-inoculated plants,indicating that AMF contributed more than DSE to plant P uptake with the same amount of C received.Moreover,plants preferentially allocated more C to AMF.These findings indicate the presence of a source-sink balance between plant C allocation and fungal P contribution.Overall,AMF and DSE conferred a higher reward to plants at the low P level through functional synergistic strategies.
基金This research was supported by the National Natural Science Foundation of China(41807335)the Shandong Provincial Natural Science Foundation,China(ZR2020MC040)+2 种基金the National Key Technology Research and Development Program of China(2019YFC0507602-2)the Youth Innovation Promotion Association of the Chinese Academy of Sciences(2020434)the National Postdoctoral Program for Innovative Talents(BX201700279).
文摘Nitrogen(N)and phosphorus(P)are two essential nutrients that determine plant growth and many nutrient cycling processes.Increasing N and P deposition is an important driver of ecosystem changes.However,in contrast to numerous studies about the impacts of nutrient addition on forests and temperate grasslands,how plant foliar stoichiometry and nutrient resorption respond to N and P addition in alpine grasslands is poorly understood.Therefore,we conducted an N and P addition experiment(involving control,N addition,P addition,and N+P addition)in an alpine grassland on Kunlun Mountains(Xinjiang Uygur Autonomous Region,China)in 2016 and 2017 to investigate the changes in leaf nutrient concentrations(i.e.,leaf N,Leaf P,and leaf N:P ratio)and nutrient resorption efficiency of Seriphidium rhodanthum and Stipa capillata,which are dominant species in this grassland.Results showed that N addition has significant effects on soil inorganic N(NO_(3)^(-)-N and NH_(4)^(+)-N)and leaf N of both species in the study periods.Compared with green leaves,leaf nutrient concentrations and nutrient resorption efficiency in senesced leaves of S.rhodanthum was more sensitive to N addition,whereas N addition influenced leaf N and leaf N:P ratio in green and senesced leaves of S.capillata.N addition did not influence N resorption efficiency of the two species.P addition and N+P addition significantly improved leaf P and had a negative effect on P resorption efficiency of the two species in the study period.These influences on plants can be explained by increasing P availability.The present results illustrated that the two species are more sensitive to P addition than N addition,which implies that P is the major limiting factor in the studied alpine grassland ecosystem.In addition,an interactive effect of N+P addition was only discernable with respect to soil availability,but did not affect plants.Therefore,exploring how nutrient characteristics and resorption response to N and P addition in the alpine grassland is important to understand nutrient use strategy of plants in terrestrial ecosystems.
基金supported by an Erasmus Mundi grant to the first author, Dr.Pramod N.Kamble
文摘Microbial growth in soil is mostly limited by lack of carbon (C). However, adding fresh, C-rich litter can induce nitrogen (N) limitation. We studied the effect of alleviating C and N limitation in high-pH (> 8) soils, soils expected to favor bacterial over fungal growth. Nitrogen limitation was induced by incubating soils amended with C-rich substrate (starch or straw) for 4 weeks. Limiting nutrients and the effects of alleviating limitation were then studied by adding C (as glucose) or N (as NH_4NO_3) and measuring microbial growth and respiration after 4 d. In non-amended, C-limited soils, adding C but not N increased both microbial respiration and bacterial growth. In N-limited, substrate-amended soils, adding C increased respiration, whereas adding N increased both microbial respiration and growth. Inducing N limitation by amending with straw was most easily detected in increased fungal growth after the addition of N, whereas with starch, only bacterial growth responded to alleviating N limitation. Compared to earlier results using a low-pH soil, the effect of substrate used to induce N limitation was more important than pH for inducing bacterial or fungal growth after alleviating N limitation. Furthermore, we found no evidence that alleviating N limitation resulted in decreased respiration concomitant with increased microbial growth in soil, suggesting no drastic changes in C use efficiency.
文摘Environments with reciprocal patchiness of resources, in which the availability of two resources such as light and soil nutrients are patchily distributed in horizontal space and negatively correlated in each patch, are common in many ecosystems. The strategies by which clonal plants adapt to this type of heterogeneous environment were examined in three stoloniferous herbs,Potentilla reptans L. var. sericophylla Franch., P. anserina L. and Halerpestes ruthenica (Jacq.) Qvcz., commonly inhabiting forest understories, grasslands and low saline meadows, respectively. As pairs of connected ramets were subjected to reciprocal patchiness of light and nutrients, stolon connection between the two ramets significantly enhanced biomass of both ramet growing in low light intensity but high soil nutrient condition (LH ramet) and ramet growing in high light intensity but low soil nutrient condition (HL ramet) as well as whole ramet pairs (consisting of LH ramets and HL ramets). Additionally, stolon connection greatly increased root/shoot ratio of LH ramet while significantly decreased that of HL ramet. The results indicate that a reciprocal transportation of resources between interconnected ramets and a functional specialization of ramets in uptake of abundant resources occurred. By resource sharing and functional specialization, clonal plants can efficiently acquire locally abundant resources and buffer the stress caused by reciprocal patchiness of resources.
基金supported by Innovation Research Project of Chinese Academy of Sciences(KZCX3-SW-418)the 100 Young Researcher Project of Chinese Academy of Sciences.
文摘In order to examine the causes of degradation of Pinus sylvestris var.mongolica plantations on sandy land,the foliar concentrations of N,P,K and C were analyzed and compared between the field grown P.sylvestris var.mongolica trees from two provenances(natural forests and plantations).The results indicated that natural tree needles had lower N,P and C concentrations,and higher K concentrations than those of plantation tree needles.For plantation tree needles,ratios of N:P,P.K and N:K increased with tree age before 45 years old;but they were not clear for the natural tree needles.Compared with the conclusions reported on Pinus spp.,we found that the foliar N and P concentrations were in the optimal range for both natural and plantation tree needles.This result suggested that N or P might not be the absolute limit factors in plant nutrient for P sylvestris var.mongolica on sandy land.However,foliar K concentrations in both natural and plantation tree needles were much lower than those reported on Pinus spp.(〉4.80 g kg-1).The N:P ratio of natural needles was in the adequate ranges,but N:P ratio of plantation needles was out of the adequate ranges.These results indicated that there was a better balanced nutrition status in the natural forest than in the plantations.If only considering the foliar nutrient concentrations of P sylvestris var.mongolica from different provenances,it might be concluded that the degradation phenomenon of P.sylvestris var.mongolica plantations was not induced by nutrition deficiency of absolute nutrients of N and P,but might be induced by other mineral nutrients or by the effectiveness of N and P nutrients.The unbalanced nutrition status and relatively quick decomposition of needles in the plantations might also contribute to the degradation.
文摘In the present paper, some novel opportunities for the development of high-efficient Si and III-V-based solar cells are considered: energy-saving environment friendly low-temperature technology of forming p-n junctions in Si (1), elaboration of structurally perfect GaAs/Ge/Si epitaxial substrates (2) and application of protective antireflecting coatings based on cubic zirconia (3). As a result: 1) New technique of forming p-n junctions in silicon has been elaborated. The technique provided easy and comparatively cheap process of production of semiconductor devices such as solar cells. The essence of the technique under the study is comprised in formation p-n junctions in silicon by a change of conductivity in the bulk of the sample occurring as a result of redistribution of the impurities, which already exists in the sample before its processing by ions. It differs from the techniques of diffusion and ion doping where change of conductivity and formation of p-n junction in the sample occur as a result of introduction of atoms of the other dopants from the outside;2) The conditions for synthesis of GaAs/Ge/Si epitaxial substrates with a thin (200 nm) Ge buffer layer featured with (1 - 2) × 105 cm-2 density of the threading dislocation in the GaAs layer. Ge buffer was obtained by chemical vapor deposition with a hot wire and GaAs layer of 1 μm thick was grown by the metal organic chemical vapor deposition. Root mean square surface roughness of GaAs layers of the less than 1 nm and good photoluminescence properties along with their high uniformity were obtained;3) The conditions ensuring the synthesis of uniform functional (buffer, insulating and protective) fianite layers on Si and GaAs substrates by means of magnetron and electron-beam sputtering have been determined. Fianite films have been shown to be suitable for the use as an ideal anti-reflecting material with high protective and anticorrosive properties.
文摘Seven-day-old maize (Zea mays) plants were grown hydroponically for ten days in deprived nutrient solutions against the corresponding control grown under full nutrition;the effects of S-, N- or P-deprivation on laminas’ mean stomatal conductance (gs), transpiration rate (E) and photosynthetic rate (A) were monitored, along with the impact on the laminas’ total dry mass (DM), water amount (W), length and surface area (Sa). Furthermore, a time series analysis of each parameter’s response ratios (Rr), i.e. the treatment’s value divided by the corresponding control’s one, was performed. Under S-deprivation, the Rr of laminas’ mean gs, E, and A presented oscillations within a ±15% fluctuation zone, notably the “control” zone, whilst those of laminas’ total DM, water amount, surface area, and length included oscillation during the first days and deviation later on, presenting deviation during d10. Under the N-deprivation conditions all Rr time courses except the A one, included early deviations from the control zone without recovering. The deviation from the control zone appeared at d4. Under P-deprivation, all Rr time courses represented oscillations within the control zone. P-deprivation’s patterns resembled those of S-deprivation. Compared to the one of the S-deprivation, the P-one’s oscillations took place within a broader zone. Linear relationships among the various Rr patterns were found between gs-E, gs-A, E-A, DM-W and DM-Sa. In conclusion, the impact of P-deprivation appeared in an early stage and included an alleviation action, the one of N-deprivation appeared early with no alleviation action, whilst that of S-deprivation appeared later, being rather weaker when compared to the impact of the P-deprivation’s impact.
文摘Attitudes regarding traditional energy sources have shifted toward renewable resources. Specifically, short-rotation woody crop supply systems have become more prevalent for biomass and biofuel production. However, a number of factors such as environmental and inherent resource availability can limit tree production. Given the intensified demand for wood biomass production, forest and plantation management practices are focusing on increasing productivity. Fertilizer application, while generally one of the least expensive silvicultural tools, can become costly if application rates exceed nutrient uptake or demand of the trees especially if it does not result in additional biomass production. We investigated the effect of water and varying levels of nitrogen application (56, 112, and 224 kg·N·haǃ·yrǃ) on nutrient content, resorption efficiency and proficiency, N:P and the relationship with ANPP, as well as leaf- and canopy-level nutrient use efficiency of nitrogen, phosphorus, and potassium for Populus deltoides, Quercus pagoda, and Platanus occidentalis. P. deltoides and P. occidentalis reached their maximum nitrogen budget with the application of water suggesting old agricultural fields may have sufficient nutrient levels to sustain short-rotation woody crops negating the application of additional nitrogen for these two species. Additionally, for P. deltoides and Q. pagoda application of nitrogen appeared to increase the uptake of phosphorus however, resorption efficiency for these two species were more similar to studies conducted on nutrient poor sites. Nutrient resorption proficiency for all three nutrients and all three species were at levels below the highest rates of nitrogen application. These findings suggest maximum biomass production may not necessarily be tied to maximum nutrient application.
基金The National Natural Science Foundation of China under contract No.41376086the Taishan Scholars Programme of Shandong Provincethe Aoshan Talents Program supported by the Pilot National Laboratory for Marine Science and Technology(Qingdao)under contract No.2015ASTP-OS08。
文摘The coastal upwelling has profound influence on the surrounding ecosystem by supplying the nutrient-replete water to the euphotic zone.Nutrient biogeochemistry was investigated in coastal waters of the eastern Hainan Island in summer 2015 and autumn 2016.From perspectives of nutrient dynamics and physical transport,the nutrient fluxes entered the upper 50 m water depth(between the mixed layer and the euphotic zone)arisen from the upwelling were estimated to be 2.5-5.4 mmol/(m^(2)·d),0.15-0.28 mmol/(m^(2)·d),and 2.2-7.2 mmol/(m^(2)·d)for dissolved inorganic nitrogen(DIN),phosphate(DIP),and dissolved silicate(DSi),respectively,which were around 6-to 12-fold those in the background area.The upwelled nutrients supported an additional plankton growth of(14.70±8.95)mg/m^(2)for chlorophyll a(Chl a).The distributions of nitrateδ^(15)N andδ^(18)O above the 300 m water depth(top of the North Pacific Intermediate Water)were different among the upwelling area,background area in summer,and the stations in autumn,and the difference of environmental and biogeochemical conditions between seasons should be the reason.The higher DIN/DIP concentration ratio,nitrate concentration anomaly,and lower nitrate isotope anomaly(Δ(15,18))in the upper ocean in summer than in autumn indicated the stronger nitrogen fixation and atmospheric deposition,and the following fixed nitrogen regeneration in summer.The higher values of Chl a and nitrateδ^(15)N andδ^(18)O within the euphotic zone in autumn than the background area in summer suggested the stronger nitrate assimilation in autumn.The differences in relatively strength of the assimilation,nitrogen fixation and atmospheric deposition,and the following remineralization and nitrification between the two seasons made the higherδ^(18)O:δ^(15)N and larger difference of enzymatic isotope fractionation factors^(15)εand^(18)εfor nitrate assimilation in summer than in autumn above the North Pacific Tropical Water.
文摘This paper investigates the current-voltage characteristics (CVC) strain of p-n-junction in a strong microwave (MW) field and shows that the deformation increases the current generated in the p-n-junction. We analyze the current-voltage characteristics of p-n-junction in which three-dimensional space (I,U,e) gives more complete information than the two-dimensional.
文摘Botanical, soil chemistry and soil microbiology variables were tested as predictors of in situ soil respiration rate in the various terrestrial habitats on sub-Antarctic Marion Island (47oS, 38oE). Inorganic P and total N concentration were the best predictors amongst the chemistry variables and bacteria plate count the best of the microbiology variables. However, while these chemistry and microbiology variables could accurately predict soil respiration rate for particular habitats, they proved inadequate predictors across the whole range of habitats. The best suite of predictors comprised only botanical variables (relative covers of five plant guilds) and accounted for 94% of the total across-habitat variation in soil respiration rate. Mean field soil respiration rates (2.1 - 15.5 mmol CO2 m-2 h-1) for habitats not influenced by seabirds or seals are similar to rates in comparable Northern Hemisphere tundra habitats. Seabird and seal manuring enhances soil respiration rates to values (up to 27.6 mmol CO2 m-2 h-1) higher than found at any tundra site. Glucose, N, P or N plus P were added to three habitats with contrasting soil types;a fellfield with mineral, nutrient-poor soil, a mire with organic, nutrient-poor soil and a shore-zone herbfield heavily manured by penguins and with organic, nutrient-rich soil. Glucose addition stimulated soil respiration in the fellfield and mire (especially the former) but not in the coastal herbfield soil. N and P, alone or together, did not stimulate respiration at any of the habitats, but adding glucose to fellfield soils that had previously been fortified with P or NP caused a similar increase in respiration rate, which was greater than the increase when adding glucose to soils fortified only with N. This suggests that fellfield soil respiration is limited by P rather than N, and that there is no synergism between the two nutrients. For the mire and coastal herbfield, adding glucose to soils previously fortified with N, P or NP did not enhance rates more than adding glucose to soils that had received no nutrient pre-treatment.
文摘Field and glasshouse experiments were conducted to study the effect of B. japonicum inoculation and phosphorus supplementation on macronutrient uptake by soybean. The treatments consisted of B. japonicum inoculation (with & without), phosphorus supplementation at the levels of 0, 20, 40 and 80 kg P·ha-1. Both treatments were replicated four times in a split plot design. The macronutrients considered were N, P, K, Ca and Mg. Results showed that inoculation with B. japonicum significantly contribute to the uptake of N, P, K, Ca and Mg in roots, shoots, pods and the whole soybean plant. Likewise, phosphorus supplementation significantly enhanced the uptake of N, P, K, Ca and Mg in roots, shoots, pods and the whole plant. The use of effective strains of rhizobia and P supplementation was an effective way of enhancing the growth of soybean, eventually the uptake of macronutrients in plant organs.
文摘The sluggish kinetics of the oxygen reduction reaction(ORR)and high over potential of oxygen evolution reaction(OER)are big challenges in the development of high-performance zinc-air batteries(ZABs)and fuel cells.In this work,we report a rational design and a simple fabrication strategy of a photo-enhanced Co single-atom catalyst(SAC)comprising g-C3N4 coupled with cobalt-nitrogen-doped hierarchical mesoporous carbon(Co-N/MPC),forming a staggered p-n heterojunction that effectively improves charge separation and enhances electrocatalytic activity.The incorporation of Co SACs and g-C3N4 synergistically optimizes the photogenerated electron-hole pair separation,significantly boosting the intrinsic ORR-OER duplex activity.Under illumination,g-C_(3)N_(4)@Co-N/MPC exhibits an outstanding ORR half-wave potential(E1/2)of 0.841 V(vs.RHE)in 0.1 mol L^(–1)KOH and a low OER overpotential of 497.4 mV(vs.RHE)at 10 mA cm^(–2)in 1 mol L^(–1)KOH.Notably,the catalyst achieves an exceptional peak power density of 850.7 mW cm^(–2)in ZABs and of 411 mW cm^(–2)even in H_(2)-air fuel cell.In addition,g-C_(3)N_(4)@Co-N/MPC-based ZABs also show remarkable cycling stability exceeding 250 h.The advanced photo-induced charge separation at the p-n heterojunction facilitates faster electron transfer kinetics,and the mass transport owing to hierarchical mesoporous structure of Co-N-C,thereby reducing the overpotential and enhancing the overall energy conversion efficiency.This work provides a new perspective on designing next-generation of single-atom dispersed oxygen reaction catalysts,paving the way for high-performance photo-enhanced energy storage and conversion systems.
基金financially supported by the National Natural Science Foundation of China(32101506)the Shenyang Top Youth Program(U35)the Youth Innovation Promotion Association of CAS(2023205)。
文摘Nitrogen(N)deficiency is a critical factor limiting natural regeneration in coastal shelterbelt forests,but the influence of different N forms on seedling establishment under varying light conditions remains poorly understood.This study investigated the effects of N forms and N concentrations on Ligustrum compactum seedlings under simulated canopy gap conditions using a three-factor design:N form(NO_(3)^(-)-N,NH_(4)^(+)-N,mixed N),N concentration(30 and 60 kg ha^(-1)a^(-1)),and light intensity(30%,60%,and 90%full sunlight).Results showed that N addition significantly promoted seedling growth,net photosynthesis rate,and water use efficiency;however,the effects varied among N forms and concentrations.Overall,NO_(3)^(-)-N or mixed N were more favored by L.compactum seedlings;however,the N preference was altered by light intensity and N concentration.For instance,L.compactum showed greater NO_(3)^(-)-N or mixed N preference under low and medium light intensities,while displaying more NH_(4)^(+)-N preference under high light intensity.N concentration also affected the growth and N preference of L.compactum seedlings,but the variance explained by N concentration was lower than that of light intensity.Leaf C,N,P stoichiometry exhibited stronger correlations with seedling's morphological trait plasticity than those of leaf gas exchange,and further analysis demonstrated that leaf C:P and N:P were the top two critical factors affecting seedling growth,indicating that the coordination and balance among C,N,P elements were more important in explaining the seedling growth under N addition.Therefore,our results clarified that the N preference of L.compactum seedlings could be altered by light intensity and revealed that leaf C,N,P ratios were stronger predictors than leaf gas exchange parameters for explaining the N effects on seedling performance.These findings demonstrated the mechanisms of light-N interactions affecting seedling performance,providing practical guidance for optimizing N fertilization and improving natural regeneration in canopy gaps of degraded coastal shelterbelt forests.
基金"Hundred Scientists"Project of Chinese Academy of Sciences.
文摘The effect of different sources and levels of N on dry matter production, nutrient uptake and ionic balance ofLarlix gmelini was studied. The results showed that the growth of the plants fertilized with ammonium was not as good as the control treatment. The growth of the plants fertilized with ammonium nitrate did not differ significantly from that in control or nitrate treatment, but was better than that in the ammonium treatment. Total cation concentrations in shoots varied little with N level in the ammonium and ammonium nitrate treatments, while those in the shoot increased with N level in the nitrate treatment. The treatments had little effect on the anion concentrations in the shoot. In the roots, the concentrations of both cations and anions changed little except for SO4 2? and Ca2+. There existed a higher carboxylate production in the plants fertilized with nitrate. The ratio between the production of carboxylate and the production of organic N Δ(C-A)/ΔNorg was constant with N supply in the plants receiving nitrate, but obviously declined with N supply for ammonium-fed plants. Δ (C-A)/ΔNorg values were intermediate between those of the nitrate and the ammonium-fed plants as for the mixed N source.
基金supported by the Natural Science Foundation of Jiangsu Province (No.BK2006710) the Hi-Tech Research and Development Program (863) of China (No:2003AA601100)
文摘Increasing levels of pollution within water bodies can cause eutrophication and an associated rapid growth in and reproduction of phytoplankton. Although most frequently occurring in bodies of water such as lakes and dams, in recent years an increasing number of river systems in China have suffered serious algal blooms. The community structure of phytoplankton may differ, however, dependent on the hydrodynamic conditions and nutrient levels within the water body. The field investigation results obtained from a stagnant river in Suzhou City and Taihu Lake, China, showed that in water with higher concentrations of nitrogen and phosphorus, Chlorophyta became the predominant species and in water with lower concentrations of nitrogen and phosphorus, Cyanobacteria became the predominant species. Growth experiments with competitive species, Microcystis aeruginosa Kutz and Scenedesmus quadricauda （Turp.）, were conducted at three different nutrient levels. The biomass of algae in pure and mixed cultures was measured under conditions of different N/P ratios at oligotrophic, eutrophic and hypertrophic nutrient levels. The results indicated that the most suitable state for the growth and reproduction of M. aeruginosa and S. quadricauda were eutrophic conditions in both pure and mixed cultures. Under competition, however, the lower medium nutrient levels favoured M. aeruginosa, while the higher medium nutrient levels better suited S. quadricauda. Under similar hydrodynamic conditions, the community structure of phytoplankton in the water body was determined by the dominant species in competition for nutrients.
