期刊文献+
共找到448篇文章
< 1 2 23 >
每页显示 20 50 100
Dynamic Responses of Super Rice IIyou602 Plants to Different Nitrogen Levels and Configuration Modes 被引量:1
1
作者 姜心禄 郑家国 +3 位作者 池忠志 李旭毅 牟中林 李涛 《Agricultural Science & Technology》 CAS 2012年第3期574-577,共4页
[Objective] The paper was to study the dynamic responses of super rice Ilyou602 plants to different nitrogen levels and configuration modes. [Method] With super rice Ilyou602 as test material, the dynamic changes of t... [Objective] The paper was to study the dynamic responses of super rice Ilyou602 plants to different nitrogen levels and configuration modes. [Method] With super rice Ilyou602 as test material, the dynamic changes of total N content in functional leaves, leaf sheaths and stems under different, application amount of nitrogen and configuration modes were analyzed. [Result] The highest total N content of functional leaves under different N levels appeared in the different periods, which was the highest during rehydration period in A3 level. The total N content in various plant positions during full heading stage and maturity stage showed fertilization treatments 〉 CK, functional leaves 〉 leaf sheaths 〉 stems. The total N content in different plant positions showed obvious difference in different treatments. When the super rice Ilyou602 was planted in Chengdu plain, the proper application level of N and configuration mode was as follows: 165 kg/hm2, fertilizer: topdressing = 6:4. [Conclusion] The study provided theoretical basis for nitrogen regulation of high yield cultivation of super hybrid rice. 展开更多
关键词 n levels Configuration modes Super rice Dynamic response
在线阅读 下载PDF
Combined effect of shading time and nitrogen level on grain filling and grain quality in japonica super rice 被引量:18
2
作者 WEI Hai-yan ZHU Ying +9 位作者 QIU Shi HAN Chao HU Lei XU Dong ZHOU Nian-bing XING Zhi-peng HU Ya-jie CUI Pei-yuan DAI Qi-gen ZHANG Hong-cheng 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2018年第11期2405-2417,共13页
There is limited information about the combined effect of shading time and nitrogen (N) on grain filling and quality of rice. Therefore, two japonica super rice cultivars, Nanjing 44 and Ningjing 3, were used to stu... There is limited information about the combined effect of shading time and nitrogen (N) on grain filling and quality of rice. Therefore, two japonica super rice cultivars, Nanjing 44 and Ningjing 3, were used to study the effect of shading time and N level on the characteristics of rice panicle and grain filling as well as the corresponding yield and quality. At a low N level (150 kg N ha^-1, 150N), grain yield decreased (by 21.07-26.07%) under the treatment of 20 days of shading before heading (BH) compared with the no shading (NS) treatment. These decreases occurred because of shortened panicle length, decreased number of primary and secondary branches, as well as the grain number and weight per panicle. At 150N, in the treatment of 20 days of shading after heading (AH), grain yield also decreased (by 9.46-10.60%) due to the lower grain weight per panicle. The interaction of shading and N level had a significant effect on the number of primary and secondary branches. A high level of N (300 kg N ha^-1, 300N) could offset the negative effect of shading on the number of secondary branches and grain weight per panicle, and consequently increased the grain yield in both shading treatments. In superior grains, compared with 150N NS, the time to reach 99% of the grain weight (T99) was shortened by 1.6 to 1.7 days, and the grain weight was decreased by 4.18-5.91% in 150N BH. In 150N AH, the grain weight was 13.39-13.92% lower than that in 150N NS due to the slow mean and the maximum grain-filling rate (GRmean and GRmax). In inferior grains, grain weight and GRmean had a tendency of 150N NS〉150N BH〉150N AH. Under shaded conditions, 300N decreased the grain weight due to lower GReen both in superior and inferior grains. Compared with 150N NS, the milling and appearance qualities as well as eating and cooking quality were all decreased in 150N BH and 150N AH. Shading with the high level of 300N improved the milling quality and decreased the number of chalky rice kernels, but the eating and cooking quality was reduced with increased chalky area and overall chalkiness. Therefore, in the case of short term shading, appropriate N fertilizer could be used to improve the yield and milling quality of rice, but limited application of N fertilizer is recommended to achieve good eating and cooking quality of rice. 展开更多
关键词 shading time n levels grain filling rice quality japonica super rice
在线阅读 下载PDF
Influence of Nitrogen Levels and Application Methods on Yield and Quality of Sorghum
3
作者 A.RASHID R.U.KHAN H.ULLAH 《Pedosphere》 SCIE CAS CSCD 2008年第2期236-241,共6页
A field study was conducted for two years at the Arid Zone Research Institute, Dera Ismail Khan, Pakistan, to determine the optimum level of nitrogen and efficient application method in the production of sorghum (Sor... A field study was conducted for two years at the Arid Zone Research Institute, Dera Ismail Khan, Pakistan, to determine the optimum level of nitrogen and efficient application method in the production of sorghum (Sorghum bicolor L.). Using four levels of nitrogen, i.e., 0, 60, 90, and 120 kg ha^-1, and two different application methods (soil application and foliar spray), the experiment was laid out in a split-plot design, where the main plots were used to determine the effective method of application and the subplots were used to detect the influence of N levels on the grain yield. The average data obtained after two years of study indicated an increase in the grain yields with an increase in N levels irrespective of the method used of N application. The grain yield increased from 2.92 to 5.61 t ha^-1 in the plots that were treated with 90 kg N ha^-1 compared with the control plots. Quadratic regression analysis showed that the increase in the yield was higher at the lower levels of N compared with the succeeding higher levels. The soil application method, producing an average grain yield of 4.79 t ha^-1, was found to be superior to the foliar spray method with an average grain yield of 4.56 t ha^-1. The protein content of the grain showed a linear increase with N application, attaining the maximum at 120 kg N ha^-1 in both the methods of N application. In addition, compared with the method of soil application, higher crude protein contents were observed using the method of foliar spray at all N levels. 展开更多
关键词 foliar spray grain yield n level protein content soil application
在线阅读 下载PDF
Integrated Action of Mixture Rates and Nitrogen Levels on Quantity and Quality of Forage Mixture from Egyptian Clover and Barley in Sandy Soil 被引量:1
4
作者 Mohamed Farouk El-Karamany Bakry Ahmed Bakry Tarek Abd El-Fattah Elewa 《Agricultural Sciences》 2014年第14期1539-1546,共8页
Effect of seeding rate mixture from Egyptian clover (Trifolium alexandrinum L.) E.C. and barley (Hordum vulgare L.) B, N-fertilization levels and their interaction on quantity and quality of forage yield. Two field ex... Effect of seeding rate mixture from Egyptian clover (Trifolium alexandrinum L.) E.C. and barley (Hordum vulgare L.) B, N-fertilization levels and their interaction on quantity and quality of forage yield. Two field experiments were conducted during winter season of 2012/2013 and 2013/2014 in sandy soil at research and production station of National Research Centre, Al Nubaria district, Al Behaira Governorate, Egypt. The experiment included two factors first was five mixtures from seeding rates (24 kg EC/fed. – 50 kg B/fed. – 18 kg EC + 12.5 kg B/fed. – 12kg EC + 25 kg B/fed. – 6 kg EC + 37.5 kg B/fed.) and second factor was three N levels (30-45-60 kg N/fed.). Two cuts were obtained at 60 and 100 DAS. Results indicated significant differences between mixture rates for all studied characters in both cuts 60 and 100 DAS. Significant differences between N levels for all studied characters at 60 DAS, also, forage yield /fed. and DM% at 100 DAS. It can be introducing forage mixture of 18 kg EC + 12.5 B had balanced character in forage yield/fed., DM%, carbohydrate % and protein % at both cuts 60 and 100 DAS. 展开更多
关键词 FORAGE MIXTURE EGYPTIAn CLOVER BARLEY n-levels
暂未订购
Effect of nutrient level on phytoplankton community structure in different water bodies 被引量:28
5
作者 Wei Zhu Lei Wan Lianfang Zhao 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2010年第1期32-39,共8页
Increasing levels of pollution within water bodies can cause eutrophication and an associated rapid growth in and reproduction of phytoplankton. Although most frequently occurring in bodies of water such as lakes and ... Increasing levels of pollution within water bodies can cause eutrophication and an associated rapid growth in and reproduction of phytoplankton. Although most frequently occurring in bodies of water such as lakes and dams, in recent years an increasing number of river systems in China have suffered serious algal blooms. The community structure of phytoplankton may differ, however, dependent on the hydrodynamic conditions and nutrient levels within the water body. The field investigation results obtained from a stagnant river in Suzhou City and Taihu Lake, China, showed that in water with higher concentrations of nitrogen and phosphorus, Chlorophyta became the predominant species and in water with lower concentrations of nitrogen and phosphorus, Cyanobacteria became the predominant species. Growth experiments with competitive species, Microcystis aeruginosa Kutz and Scenedesmus quadricauda (Turp.), were conducted at three different nutrient levels. The biomass of algae in pure and mixed cultures was measured under conditions of different N/P ratios at oligotrophic, eutrophic and hypertrophic nutrient levels. The results indicated that the most suitable state for the growth and reproduction of M. aeruginosa and S. quadricauda were eutrophic conditions in both pure and mixed cultures. Under competition, however, the lower medium nutrient levels favoured M. aeruginosa, while the higher medium nutrient levels better suited S. quadricauda. Under similar hydrodynamic conditions, the community structure of phytoplankton in the water body was determined by the dominant species in competition for nutrients. 展开更多
关键词 PHYTOPLAnKTOn community structure nutrients level n/P ratio
在线阅读 下载PDF
利用Level-Set函数求解的含表面张力项的N-S方程
6
作者 高玉丽 《莱阳农学院学报》 2003年第2期125-127,共3页
流体的温度或组成的变化,都会引起表面张力的变化。因而在研究有关自由面的流体运动中,都要考虑自由面上的表面张力。本文利用当前流行的求解自由面的LevelSet方法给出含表面张力的N-S方程的推导。
关键词 表面张力 自由面 流体运动 level-Set函数 n-S方程 曲率
在线阅读 下载PDF
Stark and Kerr Effects on the Dynamics of Moving N-Level Atomic System
7
作者 Syed Jamal Anwar Mohammed Ramzan +1 位作者 Mohammed Usman Mohammed Khalid Khan 《Journal of Quantum Information Science》 2019年第1期22-40,共19页
We have investigated numerically the dynamics of quantum Fisher information (QFI) and quantum entanglement (QE) for N-level atomic system interacting with a coherent field in the presence of Kerr (linear and non-linea... We have investigated numerically the dynamics of quantum Fisher information (QFI) and quantum entanglement (QE) for N-level atomic system interacting with a coherent field in the presence of Kerr (linear and non-linear medium) and Stark effects. It is observed that the Stark and Kerr effects play a prominent role during the time evolution of the quantum system. The evolving quantum Fisher information (QFI) is noted as time grows under the non-linear Kerr medium contrary to the QE for higher dimensional systems. The effect of non-linear Kerr medium is greater on the QE as we increase the value of Kerr parameter. However, QFI and QE maintain their periodic nature under atomic motion. On the other hand, linear Kerr medium has no prominent effects on the dynamics of N-level atomic system. Furthermore, it has been observed that QFI and QE decay soon under the influence of Stark effect. In short, the N-level atomic system is found prone to the change of the Kerr medium and Stark effect for higher dimensional systems. 展开更多
关键词 QFI KERR EFFECT STARK EFFECT n-level ATOMIC System
在线阅读 下载PDF
“1+3+N”多层次医疗保障体系协同发展研究——基于待遇给付视角 被引量:3
8
作者 胡宏伟 刘堂森 《中国医疗保险》 2025年第4期5-12,共8页
“1+3+N”多层次医疗保障体系协同发展是提升医疗保障待遇水平的重要途径。在厘清多层次医疗保障体系待遇给付次序的形成路径后发现,给付次序的优化根本上依赖于基本医保“三重保障”和“其他保障力量”各层次错位协同发展,实现“目标... “1+3+N”多层次医疗保障体系协同发展是提升医疗保障待遇水平的重要途径。在厘清多层次医疗保障体系待遇给付次序的形成路径后发现,给付次序的优化根本上依赖于基本医保“三重保障”和“其他保障力量”各层次错位协同发展,实现“目标协同、功能错位、内容互补”的目标。具体而言,“三重保障”要以制度整合为方向,持续推进医保筹资机制和支付方式改革,在“充分的保基本”目标基础上进一步促进健康公平;传统商业健康险要坚持以“多元医疗需求满足”为核心的错位发展,致力于群众差异化医疗需求保障;惠民保和职工互助保障要坚持以“基本医疗费用补偿”为核心的错位发展,弥补基本制度重特大疾病保障不足的缺口。 展开更多
关键词 1+3+n 多层次医疗保障 待遇给付 错位协同
在线阅读 下载PDF
Modulation of atomic exit and injection rates on the phase-dependent gain without inversion in a Doppler broadened open four-level system
9
作者 刘中波 贾克宁 +2 位作者 梁颖 仝殿民 樊锡君 《Chinese Physics B》 SCIE EI CAS CSCD 2012年第6期231-237,共1页
It is shown that in a Doppler broadened open N-type four-level atomic system with spontaneously generated coherence (SGC), the gain without inversion (GWI) is very sensitive to the variation of the relative phase betw... It is shown that in a Doppler broadened open N-type four-level atomic system with spontaneously generated coherence (SGC), the gain without inversion (GWI) is very sensitive to the variation of the relative phase between the probe field and the driving field; the atomic exit rate (R0) and the ratio (S) of the atomic injection rates have a considerable modulation effect on the phase-dependent GWI. GWI first increases and then decreases with R0 increasing; in a certain value range of S, GWI increases monotonically with S increasing; by adjusting the values of R0 and S, in an open system a much larger GWI can be obtained than in the corresponding closed system [2011 Phys. Rev. A 83 043805]. The modulation effects of R0 and S on the phase-dependent GWI in the case with the counter-propagating probe and driving fields are stronger than those in the co-propagating case, GWI in the co-propagating case is much larger than that in the counter-propagating case. 展开更多
关键词 open n-type four-level system spontaneously generated coherence relative phase Doppler broadening gain without inversion
原文传递
the phase-dependent gain without inversion in a Doppler broadened open four-level system
10
作者 刘中波 贾克宁 +2 位作者 梁颖 仝殿民 樊锡君 《Chinese Physics B》 SCIE EI CAS CSCD 2012年第6期227-233,共7页
It is shown that in a Doppler broadened open N-type four-level atomic system with spontaneously generated coherence (SGC), the gain without inversion (GWI) is very sensitive to the variation of the relative phase ... It is shown that in a Doppler broadened open N-type four-level atomic system with spontaneously generated coherence (SGC), the gain without inversion (GWI) is very sensitive to the variation of the relative phase between the probe field and the driving field; the atomic exit rate (R0) and the ratio (S) of the atomic injection rates have a considerable modulation effect on the phase-dependent GWI. GWI first increases and then decreases with R0 increasing; in a certain value range of S, GWI increases monotonically with S increasing; by adjusting the values of R0 and S, in an open system a much larger GWI can be obtained than in the corresponding closed system [2011 Phys. Rev. A 83 043805]. The modulation effects of R0 and S on the phase-dependent GWI in the case with the counter-propagating probe and driving fields are stronger than those in the co-propagating case, GWI in the co-propagating case is much larger than that in the counter-propagating case. 展开更多
关键词 open n-type four-level system spontaneously generated coherence relative phase Doppler broadening gain without inversion
原文传递
The Pancharatnam Phase of a Three-Level Atom Coupled to Two Systems of N-Two Level Atoms
11
作者 D. A. M. Abo-Kahla 《Journal of Quantum Information Science》 2016年第1期44-55,共12页
In this paper, we present the analytical solution for the model that describes the interaction between a three-level atom and two systems of N-two level atoms. The effects of the quantum numbers and the coupling param... In this paper, we present the analytical solution for the model that describes the interaction between a three-level atom and two systems of N-two level atoms. The effects of the quantum numbers and the coupling parameters between spins on the Pancharatnam phase and the atomic inversion, for some special cases of the initial states, are investigated. The comparison between the two effects shows that the analytic results are well consistent. 展开更多
关键词 Pancharatnam Phase Atomic Inversion Systems of n-Two level Atoms
在线阅读 下载PDF
NAC联合BiPAP无创呼吸机对COPD合并呼吸衰竭患者血气指标及炎症指标水平的影响
12
作者 朱戈 李晓倩 白兆青 《分子诊断与治疗杂志》 2025年第8期1440-1443,共4页
目的探讨N-乙酰半胱氨酸(NAC)联合双水平无创正压通气(BiPAP)无创呼吸机对慢性阻塞性肺疾病(COPD)合并呼吸衰竭患者血气指标及炎症指标水平的影响。方法回顾性分析2021年1月至2024年1月在安庆市立医院就诊的92例COPD合并呼吸衰竭患者的... 目的探讨N-乙酰半胱氨酸(NAC)联合双水平无创正压通气(BiPAP)无创呼吸机对慢性阻塞性肺疾病(COPD)合并呼吸衰竭患者血气指标及炎症指标水平的影响。方法回顾性分析2021年1月至2024年1月在安庆市立医院就诊的92例COPD合并呼吸衰竭患者的临床资料。按照治疗方式的不同分为对照组(n=50)与实验组(n=42)。对照组患者常规给予平喘、抗感染、BiPAP无创呼吸机治疗,实验组在此基础上给予NAC治疗,均持续治疗7 d。比较两组的临床疗效、肺功能指标[用力肺活量占预计值百分比(FVC%pred)、1 s用力呼气容积占预计值的百分比(FEV_(1)%pred)、FVC与FEV_(1)比值(FVC/FEV_(1))]、血气指标[动脉血氧分压(PaO_(2))、动脉血二氧化碳(PaCO_(2))、血氧饱和度(SaO_(2))]、炎症指标[降钙素原(PCT)、C反应蛋白(CRP)]以及不良反应。结果实验组治疗有效率为92.86%高于对照组78.00%,差异有统计学意义(P<0.05)。治疗7 d末实验组FEV_(1)%pred、FVC%pred、FVC/FEV_(1)、PaO_(2)、SaO_(2)显著高于同组治疗前且组间比较高于对照组同期,差异有统计学意义(P<0.05);治疗7 d末实验组PaCO_(2)、PCT、CRP显著低于同组治疗前且组间比较低于对照组同期,差异有统计学意义(P<0.05)。两组不良反应率为11.90%vs 8.00%比较差异无统计学意义(P>0.05)。结论NAC联合BiPAP无创呼吸机可提高COPD合并呼吸衰竭患者治疗有效率,改善患者肺功能、血气指标,减轻炎症反应且安全性良好。 展开更多
关键词 n-乙酰半胱氨酸 双水平无创正压通气 慢性阻塞性肺疾病 呼吸衰竭 血气指标
暂未订购
培哚普利联合氨氯地平对原发性高血压伴左心室肥厚的逆转作用及对患者血浆N末端B型利钠肽原水平的影响 被引量:7
13
作者 张金春 王朝霞 李志华 《陕西医学杂志》 CAS 2025年第1期109-113,共5页
目的:探究培哚普利联合氨氯地平对原发性高血压伴左心室肥厚的逆转作用及对血浆N末端B型利钠肽原(NT-ProBNP)水平的影响。方法:选择原发性高血压伴左心室肥厚患者138例为研究对象,按随机数表法分为三组,均46例。对照组1予以氨氯地平治疗... 目的:探究培哚普利联合氨氯地平对原发性高血压伴左心室肥厚的逆转作用及对血浆N末端B型利钠肽原(NT-ProBNP)水平的影响。方法:选择原发性高血压伴左心室肥厚患者138例为研究对象,按随机数表法分为三组,均46例。对照组1予以氨氯地平治疗,对照组2予以培哚普利治疗,观察组采用培哚普利联合氨氯地平治疗。比较临床疗效、治疗前后血压情况、舒张末期左室内径(LVDd)、室间隔厚度(I与T)、左室后壁厚度(PWT)、左室重量指数(LVMI)及NT-ProBNP、同型半胱氨酸(Hcy)心钠肽(ANP)水平。结果:观察组总有效率为95.65%(44/46)高于对照组1[80.43%(37/46)]和对照组2[76.09%(35/46)](均P<0.05),对照组1和对照组2比较无统计学差异(P>0.05)。治疗前,三组收缩压、舒张压、I与T、PWT、LVMI及血清NT-ProBNP、Hcy、ANP水平比较,差异无统计学意义(均P>0.05),治疗后,观察组收缩压(127.87±13.66与136.46±15.73与135.00±15.98)、舒张压(81.07±5.58与83.98±7.16与85.57±7.22)、LVDd(46.48±2.24与48.37±3.62与48.30±3.57)、I与T(10.00±1.38与11.20±1.50与11.24±1.55)、PWT(10.52±1.35与12.24±1.40与12.37±1.39)、LVMI(124.57±10.80与145.13±10.27与144.93±10.34)、NT-ProBNP(64.53±15.63与75.23±18.46与78.15±18.54)、Hcy(8.93±2.68与15.48±4.66与14.88±4.62)、ANP(139.95±38.64与216.84±59.15与213.87±59.23)水平均较对照组1和对照组2下降(均P<0.05),对照组1和对照组2比较无统计学差异(P>0.05)。结论:培哚普利联合氨氯地平对原发性高血压伴左心室肥厚治疗效果较好,可有效降低血压、改善左室相关指标,并降低NT-Pro BNP水平。 展开更多
关键词 培哚普利 氨氯地平 原发性高血压伴左心室肥厚 血浆n末端B型利钠肽原水平 逆转作用
暂未订购
A Bit-level Text Compression Scheme Based on the ACW Algorithm
14
作者 Hussein Al-Bahadili Shakir M. Hussain 《International Journal of Automation and computing》 EI 2010年第1期123-131,共9页
This paper presents a description and performance evaluation of a new bit-level, lossless, adaptive, and asymmetric data compression scheme that is based on the adaptive character wordlength (ACW(n)) algorithm. Th... This paper presents a description and performance evaluation of a new bit-level, lossless, adaptive, and asymmetric data compression scheme that is based on the adaptive character wordlength (ACW(n)) algorithm. The proposed scheme enhances the compression ratio of the ACW(n) algorithm by dividing the binary sequence into a number of subsequences (s), each of them satisfying the condition that the number of decimal values (d) of the n-bit length characters is equal to or less than 256. Therefore, the new scheme is referred to as ACW(n, s), where n is the adaptive character wordlength and s is the number of subsequences. The new scheme was used to compress a number of text files from standard corpora. The obtained results demonstrate that the ACW(n, s) scheme achieves higher compression ratio than many widely used compression algorithms and it achieves a competitive performance compared to state-of-the-art compression tools. 展开更多
关键词 Data compression bit-level text compression ACW(n algorithm Huffman coding adaptive coding
在线阅读 下载PDF
系统性红斑狼疮患者外周血CD14^(+)CD16^(+)单核细胞和IgG N-糖基化水平与疾病活动度的相关性研究
15
作者 刘建晓 董月稳 +4 位作者 刘向芹 陈世成 薛云 王天赐 张凯 《现代检验医学杂志》 2025年第5期88-93,共6页
目的研究系统性红斑狼疮(SLE)患者外周血CD14^(+)CD16^(+)单核细胞和IgG N-糖基化水平与疾病活动度相关性。方法回顾性选取2021年8月~2024年11月邢台市中心医院收治的109例SLE患者作为研究对象。根据SLE疾病活动指数(SLE-DAI)将患者分... 目的研究系统性红斑狼疮(SLE)患者外周血CD14^(+)CD16^(+)单核细胞和IgG N-糖基化水平与疾病活动度相关性。方法回顾性选取2021年8月~2024年11月邢台市中心医院收治的109例SLE患者作为研究对象。根据SLE疾病活动指数(SLE-DAI)将患者分为活动组(n=52)和稳定组(n=57),另选取同期进行健康体检者56例为对照组。收集纳入者临床资料,通过流式细胞仪(FCM)检测CD14^(+)CD16^(+)单核细胞,亲水相互作用色谱-质谱联用检测IgG N-糖基水平;Logistic回归分析SLE-DAI的影响因素,对自变量进行多重共线性检验[方差膨胀因子(VIF)];构建疾病活动度的预测模型,通过描绘受试者工作特征(ROC)曲线并计算曲线下面积(AUC)值评估预测模型的效能;Hosmer-Lemeshow拟合优度检验预测模型校准度。结果对照组WBC,Hb,PLT,ALB,补体C3,补体C4水平高于SLE组(t=8.917~22.171),而C反应蛋白(CRP)水平低于SLE组(t=-17.359),差异具有统计学意义(均P<0.05);活动组CRP水平和CD14^(+)CD16^(+)单个核细胞比例高于稳定组,差异具有统计学意义(t=5.449,11.112,均P<0.05);活动组IgG N-糖基化特征中半乳糖基化、唾液酸化、平分型N-乙酰葡糖胺修饰均低于稳定组,差异具有统计学意义(Z=-2.361,-2.12,-2.432,均P<0.05)。Spearman相关分析显示,CD14^(+)CD16^(+)单核细胞比例与IgG半乳糖基化、唾液酸化水平、平分型N-乙酰葡糖胺修饰呈显著负相关(r=-0.656,-0.531,-0.608,均P<0.01),CD14^(+)CD16^(+)单核细胞比例与SLE-DIA呈正相关(r=0.581,均P<0.01);IgG半乳糖基化、唾液酸化水平、平分型N-乙酰葡糖胺修饰与SLE-DIA呈负相关(r=-0.645,-0.609,-0.503,均P<0.01)。Logistic回归分析,CRP>8.21mg/L,CD14^(+)CD16^(+)≥16.17%,唾液酸化<22.05%,平分型N-乙酰葡糖胺修饰<16.53%是SLE患者疾病活动度的独立危险因素(Waldχ^(2)=4.471~12.811,均P<0.05),且上述自变量的VIF值均小于10;通过建立Logistic回归预测模型并绘制ROC曲线,计算得出诊断SLE-DIA的AUC值为0.821(95%CI:0.733~0.905),灵敏度、特异度和约登指数分别为85.37%,75.67%,0.677;Hosmer-Lemeshow拟合优度检验模型P值为0.568。结论SLE患者外周血CD14^(+)CD16^(+)单核细胞比例显著增加,IgG N-糖基化特征水平降低,均与SLE-DIA具有相关性,基于二者构建的预测模型区分SLE疾病活动与非活动状态的能力良好,高灵敏度和适中特异度利于临床早期识别,模型拟合效果好,能较准确评估SLE-DAI。 展开更多
关键词 系统性红斑狼疮 CD14^(+)CD16^(+)单核细胞 IgG n-糖基化水平 疾病活动度
暂未订购
基于离子交换色谱的高唾液酸N糖谱测定方法的建立
16
作者 尹红锐 方欣欣 +3 位作者 张颖 徐明明 郑璐侠 邵泓 《中国药品标准》 2025年第4期366-370,共5页
目的:建立基于离子交换色谱的高唾液酸N糖谱测定方法。方法:采用阴离子交换色谱柱(4.6 mm×250 mm,5μm,或等效的色谱柱);以20%乙腈溶液为流动相A,以0.1 mol·L^(-1)甲酸铵的20%乙腈溶液(pH 4.5)为流动相B,流速0.60 mL·min... 目的:建立基于离子交换色谱的高唾液酸N糖谱测定方法。方法:采用阴离子交换色谱柱(4.6 mm×250 mm,5μm,或等效的色谱柱);以20%乙腈溶液为流动相A,以0.1 mol·L^(-1)甲酸铵的20%乙腈溶液(pH 4.5)为流动相B,流速0.60 mL·min^(-1);荧光检测器检测,激发波长为330 nm、发射波长为420 nm;进样体积5μL。结果:在40%~160%的目标上样量范围内,峰面积与蛋白浓度呈良好线性(r>0.99);3个浓度水平下平均回收率分别为106.25%、100.00%、106.67%;定量限为0.03%。结论:该方法可用于不同糖蛋白药物中N-糖谱唾液酸化水平的检测。 展开更多
关键词 离子交换色谱 高唾液酸n糖谱 糖蛋白药物
暂未订购
施氮量对嘎啦幼苗^(15)N、^(13)C分配利用特性影响 被引量:7
17
作者 孙聪伟 褚凤杰 +2 位作者 杨丽丽 陈展 姜远茂 《植物营养与肥料学报》 CAS CSCD 北大核心 2015年第2期431-438,共8页
【目的】采用15N、13C同位素示踪技术,通过对不同施氮量下嘎啦幼苗生长状况及氮、碳分配、利用特性等的研究,以期为苹果生产合理施肥提供依据。【方法】将2年生盆栽嘎啦幼苗进行低、中、高三个氮水平处理,同时进行15N标记。在新梢旺长... 【目的】采用15N、13C同位素示踪技术,通过对不同施氮量下嘎啦幼苗生长状况及氮、碳分配、利用特性等的研究,以期为苹果生产合理施肥提供依据。【方法】将2年生盆栽嘎啦幼苗进行低、中、高三个氮水平处理,同时进行15N标记。在新梢旺长初始期、新梢旺长期、新梢缓长期分别进行整株13C标记,72小时后,整株解析为叶、梢、根三部分,进行15N、13C测定。样品全氮用凯氏定氮法测定,15N丰度用ZHT-03质谱计测定。13C丰度用DELTA V Advantage同位素比率质谱仪测定。【结果】1)中、高氮水平的施肥处理可在不同程度上提高整株及叶片干物质量和新梢长度。新梢旺长初始期和新梢缓长期嘎啦幼苗整株干物质量、新梢旺长期叶片干物质分配比率在中、高氮水平处理间差异不显著,中氮水平经济有效。新梢旺长期以后新梢长度以中氮>高氮>低氮,三者间差异性显著,中氮处理有利于新梢生长。2)在新梢旺长初始期,低氮处理植株叶片15N分配率达50%,比其他处理高出13个百分点左右,表明低氮处理更多的氮被叶片所利用,中氮和高氮处理间差异不显著,说明在本试验施氮条件下中氮供应水平已能满足氮素营养需求。3)新梢旺长期和新梢缓长期幼苗13C固定量均以中氮处理最高,新梢旺长初始期3个处理间根系13C分配率中氮>高氮>低氮,表明中氮处理有利于碳同化物在嘎啦幼苗中的分配。4)不同施氮量处理的嘎啦幼苗,15N利用率随施氮水平提高而降低,高氮处理对碳同化物分配没有显著贡献。【结论】低、中、高氮不同处理新梢缓长期碳同化物在各器官间的分配比较均衡,氮素水平不能影响碳同化物的分配。盆栽试验表明,中氮水平在保证营养供应的同时,能够促进新梢生长和树势健壮。 展开更多
关键词 施氮量 嘎啦 15n 13C 分配 利用
在线阅读 下载PDF
新疆棕漠土棉田施N水平对棉花产量和品质及光合特性的影响 被引量:9
18
作者 杨涛 马兴旺 +5 位作者 刘骅 许咏梅 王斌 牛新湘 杨金钰 陈宝燕 《中国农学通报》 CSCD 北大核心 2009年第23期238-243,共6页
为探明新疆内陆区干旱区棉花生育期施N肥用量,通过棕漠土棉田膜下滴灌试验,研究了在常规施N(24 kg/666.7m2)、优化施N(16 kg/666.7m2)条件下,棉花需肥比例、生产常用施肥比例、推荐施肥比例三种施N方式对棉花的产量和品质及光合特性的... 为探明新疆内陆区干旱区棉花生育期施N肥用量,通过棕漠土棉田膜下滴灌试验,研究了在常规施N(24 kg/666.7m2)、优化施N(16 kg/666.7m2)条件下,棉花需肥比例、生产常用施肥比例、推荐施肥比例三种施N方式对棉花的产量和品质及光合特性的影响。结果表明:常规施N量下的推荐施N比例可获205.24 kg/666.7m2的皮棉产量;常规施N和优化施N对棉花纤维长度、纤维强度、纤维可纺系数影响差异可达到显著水平,而对棉花纤维整齐度、伸长率的影响差异不显著;常规施N的推荐施N比例可使棉花净光合速率在盛铃期达到20μmolCO2/(m2.s),并可获得高产;常规施N和优化施N条件下,推荐N比例的棉花叶绿素SPAD值最优。 展开更多
关键词 棕漠土 膜下滴灌 n水平 产量和品质 光合特性
在线阅读 下载PDF
秸秆原料C/N比调节对沼气产气状况的影响 被引量:11
19
作者 张昌爱 刘英 +2 位作者 黄萌 袁长波 王艳芹 《山东农业科学》 2010年第1期67-70,共4页
以玉米秸秆原料为试材,研究了不同碳氮比条件对沼气产气状况的影响。结果表明,碳氮比值为27.0的N1处理,累积产气量、稳定产气周期内的日平均产气量及碳转化效率等指标均达到最大值;随着碳氮比的降低,产气开始时间延迟且产气周期缩短,累... 以玉米秸秆原料为试材,研究了不同碳氮比条件对沼气产气状况的影响。结果表明,碳氮比值为27.0的N1处理,累积产气量、稳定产气周期内的日平均产气量及碳转化效率等指标均达到最大值;随着碳氮比的降低,产气开始时间延迟且产气周期缩短,累积产气量也降低,并且碳转化效率也有所下降。 展开更多
关键词 C/n 玉米秸秆 沼气产量 碳转化率
在线阅读 下载PDF
上一页 1 2 23 下一页 到第
使用帮助 返回顶部