[Objective] The aim was to establish a stable detection method of lentivirus transgenic sheep at DNA level.[Method] The cotyledons,umbilical cord,tail tissue of newborn transgenic lambs and the body tissues of dead la...[Objective] The aim was to establish a stable detection method of lentivirus transgenic sheep at DNA level.[Method] The cotyledons,umbilical cord,tail tissue of newborn transgenic lambs and the body tissues of dead lambs were collected and used to extract DNA for PCR with primers designed for N＋D1 fragment of follistatin gene.At the same time,we detected the CMV promoter,5＇-LTR and so many other structure elements of lentiviral vector.The body tissues of dead lambs and muscle tissues of transgenic lambs in vivo were used to extract RNA for RT-PCR.[Result]The results showed that the DNA Extraction Kit was faster and more efficient than conventional method in extracting DNA and the DNA extracted with kit was easier to be amplified than that with conventional method.In order to avoid false positive caused by the interference of endogenous gene,the primers were designed for amplifying the combination of upstream of vector gene and downstream of target gene,increasing the specificity of detection.Tail tissue of newborn transgenic lambs could be used for detection and the detected results were reliable and accurate.The detection of CMV promoter,5＇-LTR and so many other structure elements of lentiviral vector provided a data support for the biological safety of transgenic animals and verify the detected results of target gene of transgenic lambs.The transcription products of RNA extracted from three of the lambs were not detected.[Conclusion] The PCR method established in our research for detecting transgenic sheep was efficient,fast and accurate.It would provide experimental basis for further detection at protein level,lay a foundation for the establishment of multi-level and systematic detection method of transgenic sheep and provide a stable technology platform for safety monitoring of transgenic sheep.展开更多
In order to discover the novel antitumor agents,a series of N,N,1-triphenyl-1Hbenzo[d]imidazol-5-amine derivatives were designed and synthesized,and the structures were characterized by IR,H-RMS,1H and 13C NMR.X-ray c...In order to discover the novel antitumor agents,a series of N,N,1-triphenyl-1Hbenzo[d]imidazol-5-amine derivatives were designed and synthesized,and the structures were characterized by IR,H-RMS,1H and 13C NMR.X-ray crystallography showed that 4c is in monoclinic system,space group P1 with a=9.209(2),b=9.533(3),c=14.097(3)?,β=102.069(3)°,V=1202.2(5)?3,Z=2,F(000)=528,μ=1.74 mm–1,S=1.024,the final R=0.0448 and wR=0.1109.The in vitro antitumor activities of target compounds were evaluated by MTT assay against human cancer cell lines K562,HL-60,HeLa and BGC-823.The target compounds demonstrated weak or moderate antitumor activities against these cell lines.展开更多
The growth of crystals of the high T c oxide superconductors has been hampe red by the complexities of the materials and their phase diagrams.The most common crys tal growth technique adopted for these oxides is the ...The growth of crystals of the high T c oxide superconductors has been hampe red by the complexities of the materials and their phase diagrams.The most common crys tal growth technique adopted for these oxides is the “flux”method,where the st arting materials are solved in a melt,which is usually formed by excess CuO and BaO or a KCl/NaCl mixture.The crystals are produced by slow cooling of the heate d solvent.This method,however,suffers from several disadvantages: (1) the crystals are contaminated with the crucible material, (2) the crystals are difficult to remove from the crucible, (3) the crystals contain flux inclusions.展开更多
目的观察和评价丁苯酞注射液治疗进展性脑卒中(PS)的有效性和安全性。方法选取2011—2014年于福建省立医院神经内科住院治疗符合纳入与排除标准的PS患者140例为研究对象。采用随机数字表法将患者分为丁苯酞组和对照组各70例。研究过程...目的观察和评价丁苯酞注射液治疗进展性脑卒中(PS)的有效性和安全性。方法选取2011—2014年于福建省立医院神经内科住院治疗符合纳入与排除标准的PS患者140例为研究对象。采用随机数字表法将患者分为丁苯酞组和对照组各70例。研究过程中失访6例,其中丁苯酞组2例,对照组4例,将其删除。丁苯酞组给予基础治疗+丁苯酞注射液治疗,对照组给予基础治疗。首诊时、进展后、治疗后14 d和治疗后90 d采用美国国立卫生研究院卒中量表(NIHSS)评分以及NIHSS评分进展幅度评估神经功能障碍程度;治疗后14 d和治疗后90 d采用NIHSS评分演变情况评估神经功能转归情况;治疗后14 d和治疗后90 d采用Barthel指数评估治疗后日常生活能力;治疗后90 d应用改良Rankin量表(mRS)评分评估神经功能障碍康复程度,将mRS评分≤2.0分定义为预后良好,mRS评分>2.0分定义为预后不良事件。对两组脑梗死病灶同侧颈内动脉系统的狭窄闭塞性病变患者的NIHSS评分、Barthel指数、mRS评分进行比较分析。观察有无皮疹等过敏反应及其他不良事件。结果两组治疗后14 d、治疗后90 d NIHSS评分均低于进展后,治疗后90 d NIHSS评分均低于治疗后14 d(P<0.05)。丁苯酞组治疗后14 d、治疗后90 d NIHSS评分降低率均高于对照组(P<0.05)。丁苯酞组治疗后14 d、治疗后90 d Barthel指数、预后良好率高于对照组,治疗后90 d mRS评分低于对照组(P<0.05)。共发现61例(45.5%)脑梗死病灶同侧颈内动脉系统的狭窄闭塞性病变,其中丁苯酞组29例(42.6%),对照组32例(48.5%)。丁苯酞组脑梗死病灶同侧颈内动脉系统的狭窄闭塞性病变患者治疗后14 d、治疗后90 d NIHSS评分低于进展后,治疗后90 d NIHSS评分低于治疗后14 d(P<0.05)。丁苯酞组脑梗死病灶同侧颈内动脉系统的狭窄闭塞性病变患者治疗后14 d Barthel指数、治疗后90 d Barthel指数、预后良好率均高于对照组(P<0.05)。两组不良事件发生率比较,差异无统计学意义(P>0.05)。结论丁苯酞注射液治疗能够促进PS患者神经功能和预后的改善,提高生活能力,是一种有效并安全的治疗方法。展开更多
目的观察丁苯酞氯化钠注射液治疗急性期脑梗死患者的临床疗效,以及定量脑电图对急性期脑梗死患者临床疗效的监测意义。方法将80例急性脑梗死患者随机分为治疗组和对照组,治疗组在常规治疗的基础上给予丁苯酞氯化钠注射液治疗。比较两组...目的观察丁苯酞氯化钠注射液治疗急性期脑梗死患者的临床疗效,以及定量脑电图对急性期脑梗死患者临床疗效的监测意义。方法将80例急性脑梗死患者随机分为治疗组和对照组,治疗组在常规治疗的基础上给予丁苯酞氯化钠注射液治疗。比较两组治疗前后美国国立卫生研究院卒中量表(National Institutes of Health Stroke Scale,NIHSS)评分、Barthel指数评分和定量脑电图(quantitative electroencephalogram,QEEG)慢波化比率(Deltaratio,Symmetry theta/alpha+beta rate,DTABR)等数据。结果治疗14 d后,治疗组NIHSS评分[(3.58±2.72)分vs(6.33±2.63)分]、Barthel指数评分[(68.20±26.83)分vs(66.12±24.45)分]、DTABR[(2.83±1.20)分vs(4.10±1.72)分]的变化优于对照组,差异有统计学意义。结论丁苯酞氯化钠注射液治疗急性脑梗死患者临床疗效显著且定量脑电图对急性脑梗死患者临床疗效的监测有价值。展开更多
基金Supported by National Major Transgenic Project (2013ZX08008-003-04,2013ZX08010004-009)~~
文摘[Objective] The aim was to establish a stable detection method of lentivirus transgenic sheep at DNA level.[Method] The cotyledons,umbilical cord,tail tissue of newborn transgenic lambs and the body tissues of dead lambs were collected and used to extract DNA for PCR with primers designed for N＋D1 fragment of follistatin gene.At the same time,we detected the CMV promoter,5＇-LTR and so many other structure elements of lentiviral vector.The body tissues of dead lambs and muscle tissues of transgenic lambs in vivo were used to extract RNA for RT-PCR.[Result]The results showed that the DNA Extraction Kit was faster and more efficient than conventional method in extracting DNA and the DNA extracted with kit was easier to be amplified than that with conventional method.In order to avoid false positive caused by the interference of endogenous gene,the primers were designed for amplifying the combination of upstream of vector gene and downstream of target gene,increasing the specificity of detection.Tail tissue of newborn transgenic lambs could be used for detection and the detected results were reliable and accurate.The detection of CMV promoter,5＇-LTR and so many other structure elements of lentiviral vector provided a data support for the biological safety of transgenic animals and verify the detected results of target gene of transgenic lambs.The transcription products of RNA extracted from three of the lambs were not detected.[Conclusion] The PCR method established in our research for detecting transgenic sheep was efficient,fast and accurate.It would provide experimental basis for further detection at protein level,lay a foundation for the establishment of multi-level and systematic detection method of transgenic sheep and provide a stable technology platform for safety monitoring of transgenic sheep.
基金Supported by the “Climbing Program”(pdjh2019b0497)Special Funds,the Foundation of the Department of Education of Guangdong Province(2018KZDXM070,2017KZDXM085,2017KQNCX204)College Students Innovation and Entrepreneurship Training Program of Wuyi University and Foundation for Young Talents(2018KQNCX272,2018KQNCX273)
文摘In order to discover the novel antitumor agents,a series of N,N,1-triphenyl-1Hbenzo[d]imidazol-5-amine derivatives were designed and synthesized,and the structures were characterized by IR,H-RMS,1H and 13C NMR.X-ray crystallography showed that 4c is in monoclinic system,space group P1 with a=9.209(2),b=9.533(3),c=14.097(3)?,β=102.069(3)°,V=1202.2(5)?3,Z=2,F(000)=528,μ=1.74 mm–1,S=1.024,the final R=0.0448 and wR=0.1109.The in vitro antitumor activities of target compounds were evaluated by MTT assay against human cancer cell lines K562,HL-60,HeLa and BGC-823.The target compounds demonstrated weak or moderate antitumor activities against these cell lines.
文摘The growth of crystals of the high T c oxide superconductors has been hampe red by the complexities of the materials and their phase diagrams.The most common crys tal growth technique adopted for these oxides is the “flux”method,where the st arting materials are solved in a melt,which is usually formed by excess CuO and BaO or a KCl/NaCl mixture.The crystals are produced by slow cooling of the heate d solvent.This method,however,suffers from several disadvantages: (1) the crystals are contaminated with the crucible material, (2) the crystals are difficult to remove from the crucible, (3) the crystals contain flux inclusions.
文摘目的观察和评价丁苯酞注射液治疗进展性脑卒中(PS)的有效性和安全性。方法选取2011—2014年于福建省立医院神经内科住院治疗符合纳入与排除标准的PS患者140例为研究对象。采用随机数字表法将患者分为丁苯酞组和对照组各70例。研究过程中失访6例,其中丁苯酞组2例,对照组4例,将其删除。丁苯酞组给予基础治疗+丁苯酞注射液治疗,对照组给予基础治疗。首诊时、进展后、治疗后14 d和治疗后90 d采用美国国立卫生研究院卒中量表(NIHSS)评分以及NIHSS评分进展幅度评估神经功能障碍程度;治疗后14 d和治疗后90 d采用NIHSS评分演变情况评估神经功能转归情况;治疗后14 d和治疗后90 d采用Barthel指数评估治疗后日常生活能力;治疗后90 d应用改良Rankin量表(mRS)评分评估神经功能障碍康复程度,将mRS评分≤2.0分定义为预后良好,mRS评分>2.0分定义为预后不良事件。对两组脑梗死病灶同侧颈内动脉系统的狭窄闭塞性病变患者的NIHSS评分、Barthel指数、mRS评分进行比较分析。观察有无皮疹等过敏反应及其他不良事件。结果两组治疗后14 d、治疗后90 d NIHSS评分均低于进展后,治疗后90 d NIHSS评分均低于治疗后14 d(P<0.05)。丁苯酞组治疗后14 d、治疗后90 d NIHSS评分降低率均高于对照组(P<0.05)。丁苯酞组治疗后14 d、治疗后90 d Barthel指数、预后良好率高于对照组,治疗后90 d mRS评分低于对照组(P<0.05)。共发现61例(45.5%)脑梗死病灶同侧颈内动脉系统的狭窄闭塞性病变,其中丁苯酞组29例(42.6%),对照组32例(48.5%)。丁苯酞组脑梗死病灶同侧颈内动脉系统的狭窄闭塞性病变患者治疗后14 d、治疗后90 d NIHSS评分低于进展后,治疗后90 d NIHSS评分低于治疗后14 d(P<0.05)。丁苯酞组脑梗死病灶同侧颈内动脉系统的狭窄闭塞性病变患者治疗后14 d Barthel指数、治疗后90 d Barthel指数、预后良好率均高于对照组(P<0.05)。两组不良事件发生率比较,差异无统计学意义(P>0.05)。结论丁苯酞注射液治疗能够促进PS患者神经功能和预后的改善,提高生活能力,是一种有效并安全的治疗方法。
文摘目的观察丁苯酞氯化钠注射液治疗急性期脑梗死患者的临床疗效,以及定量脑电图对急性期脑梗死患者临床疗效的监测意义。方法将80例急性脑梗死患者随机分为治疗组和对照组,治疗组在常规治疗的基础上给予丁苯酞氯化钠注射液治疗。比较两组治疗前后美国国立卫生研究院卒中量表(National Institutes of Health Stroke Scale,NIHSS)评分、Barthel指数评分和定量脑电图(quantitative electroencephalogram,QEEG)慢波化比率(Deltaratio,Symmetry theta/alpha+beta rate,DTABR)等数据。结果治疗14 d后,治疗组NIHSS评分[(3.58±2.72)分vs(6.33±2.63)分]、Barthel指数评分[(68.20±26.83)分vs(66.12±24.45)分]、DTABR[(2.83±1.20)分vs(4.10±1.72)分]的变化优于对照组,差异有统计学意义。结论丁苯酞氯化钠注射液治疗急性脑梗死患者临床疗效显著且定量脑电图对急性脑梗死患者临床疗效的监测有价值。
