Long non-coding(lnc)RNA plays important roles in many cellular processes.The function of the vast majority of lncRNAs remains unknown.Here we identified that lncRNA-1700113A16RIK existed in skeletal muscle stem cells(...Long non-coding(lnc)RNA plays important roles in many cellular processes.The function of the vast majority of lncRNAs remains unknown.Here we identified that lncRNA-1700113A16RIK existed in skeletal muscle stem cells(MuSCs)and was significantly elevated during MuSC differentiation.Knockdown of 1700113A16RIK inhibits the differentiation of muscle stem cells.In contrast,overexpression of 1700113A16RIK promotes the differentiation of muscle stem cells.Further study shows the muscle specific transcription factor Myogenin(MyoG)positively regulates the expression of 1700113A16RIK by binding to the promoter region of 1700113A16RIK.Mechanistically,1700113A16RIK may regulate the expression of myogenic genes by directly binding to 3’UTR of an important myogenic transcription factor MEF2D,which in turn promotes the translation of MEF2D.Taken together,our results defined 1700113A16RIK as a positive regulator of MuSC differentiation and elucidated a mechanism as to how 1700113A16RIK regulated MuSC differentiation.展开更多
基金This work was supported by the Strategic Priority Research Program of the Chinese Academy of Science(XDA16020400 to P.H.)Ministry of Science and Technology of China(2017YFA0102700 to P.H.)+2 种基金the National Natural Science Foundation of China(32170804 to P.H.and 81200355 to W.Y.)CAS-Youth Innovation Program Association(2016246 to W.Y.)Shanghai Natural Science Foundation(18ZR1446300 to W.Y.).
文摘Long non-coding(lnc)RNA plays important roles in many cellular processes.The function of the vast majority of lncRNAs remains unknown.Here we identified that lncRNA-1700113A16RIK existed in skeletal muscle stem cells(MuSCs)and was significantly elevated during MuSC differentiation.Knockdown of 1700113A16RIK inhibits the differentiation of muscle stem cells.In contrast,overexpression of 1700113A16RIK promotes the differentiation of muscle stem cells.Further study shows the muscle specific transcription factor Myogenin(MyoG)positively regulates the expression of 1700113A16RIK by binding to the promoter region of 1700113A16RIK.Mechanistically,1700113A16RIK may regulate the expression of myogenic genes by directly binding to 3’UTR of an important myogenic transcription factor MEF2D,which in turn promotes the translation of MEF2D.Taken together,our results defined 1700113A16RIK as a positive regulator of MuSC differentiation and elucidated a mechanism as to how 1700113A16RIK regulated MuSC differentiation.
