Academic exchanges,training,and technology transfer under China-Africa cooperation are heralding a sea change in rural Tanzania.Iden Revocatus Stephano,based in Morogoro Region,is a shining example of this youth-drive...Academic exchanges,training,and technology transfer under China-Africa cooperation are heralding a sea change in rural Tanzania.Iden Revocatus Stephano,based in Morogoro Region,is a shining example of this youth-driven transition.A graduate of Sokoine University of Agriculture,26-year-old Stephano runs a flourishing mushroom farm-a testament to the growing wave of young Africans reshaping rural economies with global insight and local innovation.展开更多
Based on Taylor's model and Hawkyard's model, a new simple model for the mushrooming deformation of projectile impacting on a deformable target is installed considering the penetration of the projectile to the...Based on Taylor's model and Hawkyard's model, a new simple model for the mushrooming deformation of projectile impacting on a deformable target is installed considering the penetration of the projectile to the deformable target. In the model, the following time-dependent variables are involved in: the extent and the particle velocity in the rigid zone; the extent, the cross-section area and the particle velocity in plastic zone; the velocity and depth of the penetrating of projectile to the target. Solving the set of equations, analytic solution is given. The profiles of deformed projectile and shape parameters for different initial impact velocities are shown. The duration time of deformation increases with increasing the impact velocity. The analytical results by using this model are coincident with experimental result.展开更多
Rwandans benefit from Chinese agriculture techniques in both income generation and nutritional value A GROUP of 48 Rwandans were recently trained in mushroom growing,sericulture,soil erosion and upland rice technology...Rwandans benefit from Chinese agriculture techniques in both income generation and nutritional value A GROUP of 48 Rwandans were recently trained in mushroom growing,sericulture,soil erosion and upland rice technology,using Chinese knowhow.Mushroom growing technique is part of the展开更多
Target occlusion poses a significant challenge in computer vision,particularly in agricultural applications,where occlusion of crops can obscure key features and impair the model’s recognition performance.To address ...Target occlusion poses a significant challenge in computer vision,particularly in agricultural applications,where occlusion of crops can obscure key features and impair the model’s recognition performance.To address this challenge,a mushroom recognition method was proposed based on an erase module integrated into the EL-DenseNet model.EL-DenseNet,an extension of DenseNet,incorporated an erase attention module designed to enhance sensitivity to visible features.The erase module helped eliminate complex backgrounds and irrelevant information,allowing the mushroom body to be preserved and increasing recognition accuracy in cluttered environments.Considering the difficulty in distinguishing similar mushroom species,label smoothing regularization was employed to mitigate mislabeling errors that commonly arose from human observers.This strategy converted hard labels into soft labels during training,reducing the model’s overreliance on noisy labels and improving its generalization ability.Experimental results showed that the proposed EL-DenseNet,when combined with transfer learning,achieved a recognition accuracy of 96.7%for mushrooms in occluded and complex backgrounds.Compared with the original DenseNet and other classic models,this approach demonstrated superior accuracy and robustness,providing a promising solution for intelligent mushroom recognition.展开更多
A substantial body of research has been conducted with the objective of enhancing the understanding of Non-Timber Forest Products (NTFP) in Central Africa. A significant proportion of the studies focus on medicinal pl...A substantial body of research has been conducted with the objective of enhancing the understanding of Non-Timber Forest Products (NTFP) in Central Africa. A significant proportion of the studies focus on medicinal plants, in contrast to those that examine food products. Nevertheless, studies on edible wild mushrooms as NTFPs and cultivated mushrooms, on their economic potential and value chains in the Great Lakes Region, remain scarce and largely absent for some NTFPs. In light of the aforementioned considerations, the present study aims to examine the economic potential and value chain of the production sector of wild edible mushrooms (WEM) and cultivated mushrooms (CM) within the Virunga National Park (PNVi) and its surrounding areas in North Kivu, Democratic Republic of the Congo (DRC). To this end, a sample of 432 respondents was selected, distributed equitably among the actors of the Goma-Kitshanga-Mweso value chain, the Goma-Rutshuru chain, the Beni-Mutwanga chain, and the Beni-Mangina chain. A digital questionnaire consisting of open and closed questions was administered, and observations were carried out concurrently. The quantities of mushrooms sold were weighed and photographed. The results demonstrated that, when the eight-month fruiting period of wild and cultivated mushrooms was considered as a single year for all actors within the value chain, a picker could sell 6.1 kg at $2 and earn $12. A seller could sell 16 kg at $4 and earn $64, while a grower could sell 502 kg at $3 and earn $1506. Consequently, the CM grower generates a significantly higher profit than other participants in the value chain, despite the expenses he incurs. Ultimately, the domestication of WEM, highly prized by the local population of the Virunga National Park, has the potential to enhance their household incomes, as evidenced by the findings of this study.展开更多
The increasing demand for renewable energy sources has driven the exploration of innovative materials for biofuel production.This study investigates bio-pellet characteristics derived from several oyster mushroom bagl...The increasing demand for renewable energy sources has driven the exploration of innovative materials for biofuel production.This study investigates bio-pellet characteristics derived from several oyster mushroom baglog wastes with varying concentrations of molasses as an adhesive.The process began with sun-drying the baglog waste for three days,followed by oven drying at 80○C for 24 h.Bio-pellets were produced by blending baglog waste with molasses at concentrations of 5%and 10%(w/v),then subsequently fed into a pellet mill.The bio-pellets were left to rest for one hour before analysis.The quality of bio-pellets was determined by evaluating moisture content,ash content,volatile matter,calorific value,combustion rate,density,and compressive strength following SNI 8675:2018 standards.Results indicate that adding molasses as a binder significantly affected the bio-pellet quality.The optimal molasses concentration for balanced performance was found at 5%,providing a lowermoisture content(6.8%),volatile matter(68.42%),and density(1.55 g⋅cm−3).In addition,the bio-pellet has a slightly higher calorific value(approximately 3614 cal⋅g−1),compressive strength(40.68 N⋅mm−2),and ash content(18.59%).All of the parameters for the bio-pellet containing 5%molasses satisfied the standard except for ash content and calorific value.展开更多
Traditional taxonomic sorting of samples into recognizable taxonomic units, such as morphospecies or morphotypes, is commonly relied upon in conservation biology and ethnobiological studies. However, understanding the...Traditional taxonomic sorting of samples into recognizable taxonomic units, such as morphospecies or morphotypes, is commonly relied upon in conservation biology and ethnobiological studies. However, understanding the criteria used for traditional nomenclature of fungi, particularly wild edible mushrooms across linguistic groups, remains limited, leading to frequent errors in species recognition. This study seeks to assess how linguistic affiliations influence the local naming of useful wild mushrooms, and is the first of its kind in Benin. In order to understand how local people recognize, classify and name mushrooms that develop in or close to their villages, 2234 respondents from five socio-linguistic groups across three geographical areas were interviewed. Structured and semi-structured interviews were conducted to gather data on the local naming criteria for edible wild mushrooms. Citation scores were recorded for both nomenclature criteria and species, considering variables such as linguistic groups, age, and language. Twenty-two nomenclature criteria were used by local people to name edible wild species. Strong similarity in classification and naming of species was shown in 97% of the languages, while 3% showed differing classification criteria. The Gur, Atlantic, and Mande linguistic groups demonstrated more comprehensive traditional taxonomic and nomenclatural knowledge, sharing six common criteria: texture, taste, size, kingdom (Fungi), form, and substrate. Overall, local populations possess extensive knowledge regarding the diversity of wild edible mushrooms in their environment.展开更多
Edible mushroom proteins are the promising ones with the advantages of complete essential amino acid profile and multiple functional activities.To reinforce their applications in functional food development,this study...Edible mushroom proteins are the promising ones with the advantages of complete essential amino acid profile and multiple functional activities.To reinforce their applications in functional food development,this study comprehensively evaluated the physicochemical and functional properties of protein isolates from 5 mushroom species,i.e.,Pleurotus eryngii(PEP),Pleurotus ostreatus(POP),Lentinula edodes(LEP),Flammulina velutipes(FVP)and Hypsizygus marmoreus(HMP).Results showed that PEP,LEP,FVP,POP and HMP exhibited better protein solubility(PS),water holding capacity(WHC),emulsification activity index(EAI),and foaming capacity(FC)than those of soybean protein and pea protein isolates(PPI).PEP(51.95%)and POP(49.15%)had a higher amount ofβ-sheet structure.Principal component analysis and correlation analysis showed that the seven proteins could be divided into 3 clusters,and WHC,EAI and FC were significantly positively correlated with PS andβ-sheet.The least gelation concentration of PEP(16%)and FVP(16%)at p H 6.0 and 7.0 was similar to PPI,and PEP showed better hardness,springiness and rheological properties than other proteins gels.Overall,our study showed that 5 edible mushroom proteins possessed excellent functionalities(except for gelling capacity),which provided novel insights on unexploited sources of mushroom proteins used as protein-based foods in the food industry.展开更多
To solve the problems of redundant feature information,the insignificant difference in feature representation,and low recognition accuracy of the fine-grained image,based on the ResNeXt50 model,an MSFResNet network mo...To solve the problems of redundant feature information,the insignificant difference in feature representation,and low recognition accuracy of the fine-grained image,based on the ResNeXt50 model,an MSFResNet network model is proposed by fusing multi-scale feature information.Firstly,a multi-scale feature extraction module is designed to obtain multi-scale information on feature images by using different scales of convolution kernels.Meanwhile,the channel attention mechanism is used to increase the global information acquisition of the network.Secondly,the feature images processed by the multi-scale feature extraction module are fused with the deep feature images through short links to guide the full learning of the network,thus reducing the loss of texture details of the deep network feature images,and improving network generalization ability and recognition accuracy.Finally,the validity of the MSFResNet model is verified using public datasets and applied to wild mushroom identification.Experimental results show that compared with ResNeXt50 network model,the accuracy of the MSFResNet model is improved by 6.01%on the FGVC-Aircraft common dataset.It achieves 99.13%classification accuracy on the wild mushroom dataset,which is 0.47%higher than ResNeXt50.Furthermore,the experimental results of the thermal map show that the MSFResNet model significantly reduces the interference of background information,making the network focus on the location of the main body of wild mushroom,which can effectively improve the accuracy of wild mushroom identification.展开更多
This study examines the effects of germination substrates incorporating spent mushroom substrate(SMS)and locally sourced organic materials on the growth and vigor of cocoa seedlings.Seven treatments were evaluated usi...This study examines the effects of germination substrates incorporating spent mushroom substrate(SMS)and locally sourced organic materials on the growth and vigor of cocoa seedlings.Seven treatments were evaluated using a Completely Randomized Design(CRD),comprising combinations of coconut coir,rice husk,sugarcane bagasse,and biochar.Key findings reveal that treatments combining SMS,sugarcane bagasse,and either coconut coir or biochar(T6 and T7)achieved significantly higher survival rates and Dickson Quality Index(DQI)scores compared to conventional soil(T1).These treatments provided favorable chemical properties,including optimal pH,electrical conductivity,and nutrient content(N,P,K),which are critical for cocoa seedling development.The results suggest that using SMS with agricultural waste as an alternative germination medium can reduce cultivation costs,enhance seedling growth,and contribute to sustainable agricultural practices by repurposing organic waste.This approach not only offers cost-effective benefits for farmers but also mitigates environmental impacts associated with waste disposal.展开更多
The mechanisms of mushroom polysaccharides on immune functions and lipid metabolism of aged mammals have not been fully elucidated.In the present study,after assessing the impacts of one type of Lentinula edodes-deriv...The mechanisms of mushroom polysaccharides on immune functions and lipid metabolism of aged mammals have not been fully elucidated.In the present study,after assessing the impacts of one type of Lentinula edodes-derived polysaccharides,named L2,on immune functions and blood lipid profiles,isobaric tags for relative and absolute quantification(iTRAQ)-based proteomic profiling of the small intestinal tissues from aged mice treated with L2 was performed.L2 reversed immune function declines and modulated the lipid metabolism of aged mice evidenced by increased levels of serum TC,HDL-C,and LDL-C,and reduced levels of serum TG.Moreover,a total of 95 differentially regulated proteins(DRPs) were identified,of which75 were up-regulated and 20 were down-regulated.Most of the DRPs were involved in intracellular and extracellular structure organization,and cellular and metabolic regulation.Particularly,approximately 16 and 9 DRPs participated in the regulation of immune functions and lipid metabolism,respectively.Furthermore,protein-protein interaction analysis highlighted that cadherin-1,plectin,cadherin-17,Ras GTPase-activating-like protein IQGAP2,and ezrin might be key proteins in response to L2 treatment.These findings provide new insights into the biological mechanisms of mushroom polysaccharides in anti-aging from a proteomic perspective.展开更多
The accumulation of heavy metals in mushrooms has presented a significant risk to human health,underscoring the importance of devising a portable and cost-effective method for detecting heavy metals.Thus,we have devel...The accumulation of heavy metals in mushrooms has presented a significant risk to human health,underscoring the importance of devising a portable and cost-effective method for detecting heavy metals.Thus,we have developed an electrochemical sensor based on 3-dimensional highly reduced graphene oxide(3D-HRGO)in conjunction with Fe_(3)O_(4)nanoparticles,enabling the simultaneous quantification of Cd^(2+),Pb^(2+),Cu^(2+),and Hg^(2+).The 3D-HRGO/Fe_(3)O_(4)nano-particles material prepared in this study was characterized and confirmed by multiple techniques,then dispersed in a simple and environmental dispersant,consist of 75%ethanol and 0.1%Nafion,and coating on a glass carbon electrode(GCE)to preparing a 3D-HRGO/Fe_(3)O_(4)/GCE sensor.The limit of detection(LOD)of 3D-HRGO/Fe_(3)O_(4)/GCE sensor for Cd^(2+),Pb^(2+),Cu^(2+),and Hg^(2+)in simultaneous detection were 0.2,0.6,0.6,and 0.9μg/L,respectively.The sensor demonstrates exceptional stability,reproducibility,anti-interference,and recovery rate.Furthermore,the electrochemical sensor was employed to detect heavy metals in actual mushrooms and validated through conventional methodologies.This study represents the pioneering utilization of 3D-HRGO/Fe_(3)O_(4)as a foundational material for an electrochemical sensor capable of simultaneous detection of multiple metals,thereby advancing the progress of on-site and expeditious detection techniques.展开更多
[Background]Hygrophorus,belonging to Basidiomycota,Agaricomycetes,Agaricales,Hygrophoraceae,is widely distributed all around the world.[Objective]To investigate the species of Hygrophorus and enrich the knowledge abou...[Background]Hygrophorus,belonging to Basidiomycota,Agaricomycetes,Agaricales,Hygrophoraceae,is widely distributed all around the world.[Objective]To investigate the species of Hygrophorus and enrich the knowledge about the species diversity of this genus in Shanxi Province.[Methods]We collected fungal specimens,observed and characterized their morphology.After DNA extraction,the internal transcribed spacer(ITS)was sequenced.Phylogenetic trees were constructed based on the maximum likelihood method and Bayesian inference.[Results]Eleven species of Hygrophorus were identified based on the morphological and molecular evidence,including ten known species and a new species.This article described the macroscopic and microscopic morphological characteristics of the new species Hygrophorus brunneodiscoides in detail.The basidiocarp photos of ten known species and a key to the species of Hygrophorus in Shanxi Province were provided.[Conclusion]We identified a new species,H.brunneodiscoides,in China,confirmed 11 species of Hygrophorus in Shanxi Province,and briefed their geographical distribution and associations with hosts according to the field observation.展开更多
Novel substrates consisted of different fresh agro-industrial residues,their corresponding and commercial spent mushroom substrates(i.e.SMS deriving from laboratory-scale experiments and SMS deriving from industrial-s...Novel substrates consisted of different fresh agro-industrial residues,their corresponding and commercial spent mushroom substrates(i.e.SMS deriving from laboratory-scale experiments and SMS deriving from industrial-scale experiments by Green Zin S.A.-SMS GZ)and Pleurotus waste(PW;stipes/mishappen mushrooms)were used in blends for a new cultivation cycle of Pleurotus ostreatus and P.eryngii mushrooms in bags.Their impact on the biochemical properties(intra-cellular polysaccharides-IPSs,protein,lipid,total phenolic compounds-TPCs,individual carbohydrates composition of the IPSs)in the first-and second-flush whole mushrooms,pilei and stipes,as well as the fatty acids composition,the antioxidant activity(in the first-flush mushroom parts)and glucan content of stipes were examined.Both species produced satisfactory IPSs quantities in all substrates(28.69-46.38%,w/w)and significant protein amounts(18.37-26.80%,w/w).The further SMS addition(80%,w/w instead of 40%,w/w)in the cultivation substrates affected positively the mushroom IPSs values,whereas the highest protein content was detected in mushroom’s parts cultivated on substrates consisted of fresh agro-industrial residues.Mushroom’s lipid content was affected differently by the various substrate combinations,with SMS presence resulting in mushrooms with a lower fat content than those produced in substrates with PW addition.Fresh substrates with PW and those with coffee residue were the most favorable for TPCs production.Regarding production flushes,the nutritional value of mushrooms was comparable between them,only a slight increase in TPCs of second-flush carposomes was detected.Glucose was the predominant monosaccharide of the produced IPSs,combined with a significant production of total and β-glucans.SMSs and PW addition had a positive impact on antioxidant activity,too.A higher quantity of lipids,TPCs and significant antioxidant activity were detected in all Pleurotus pilei than stipes,whereas the latter were richer in IPSs.Both pilei and stipes had a significant protein amount.Hence,the data obtained by this study support the positive effect of different types of SMS and mushroom waste on P.ostreatus and P.eryngii nutritional value.展开更多
The oyster mushroom(Pleurotus spp.)is one of the most widely cultivated mushroom species globally.The present study investigated the effect of synbiotics on the growth and quality of Pleurotus ostreatus and Pleurotus ...The oyster mushroom(Pleurotus spp.)is one of the most widely cultivated mushroom species globally.The present study investigated the effect of synbiotics on the growth and quality of Pleurotus ostreatus and Pleurotus pulmonarius.Different synbiotics formulations were applied by spraying mushroom samples daily and measuring their growth parameters,yield,biological efficiency,proximate composition,mineral content,total phenolic content(TPC),and diphenyl-1-picryl-hydrazyl(DPPH)radical scavenging activity.Results demonstrated that the most significant yield of oyster mushrooms was harvested from synbiotics sprayed with inulin and Lactobacillus casei(56.92 g).Likewise,the highest biological efficiency obtained with a similar synbiotic was 12.65%.Combining inulin and L.casei was the most effective method of improving the mushrooms'growth performance and nutrient content in both samples.Furthermore,synbiotics that combined inulin and L.casei resulted in the highest TPC(20.550 mg gallic acid equivalent(GAE)/g dry extract(DE))in white oyster mushrooms(P.ostreatus).In comparison,in grey mushroom(P.pulmonarius)the highest TPC was yielded by L.casei(1.098 mg GAE/g DE)followed by inulin and L.casei(1.079 mg GAE/g DE).The DPPH results indicated that the oyster mushroom could be an efficient antioxidant.The results revealed that applying synbiotics improved the mushrooms'quality by increasing their antioxidant capacity with higher amounts of phenolic compounds and offering better health benefits with the increased levels of mineral elements.Together,these studies demonstrated the potential of using synbiotics as a biofertilizer,which is helpful for mushroom cultivation;therefore,it might solve the challenge of inconsistent quality mushroom growers face.展开更多
Type 1 diabetes mellitus(T1DM) lacks insulin secretion due to autoimmune deficiency of pancreaticβ-cells.Protecting pancreatic islets and enhancing insulin secretion has been therapeutic approaches.Mannogalactoglucan...Type 1 diabetes mellitus(T1DM) lacks insulin secretion due to autoimmune deficiency of pancreaticβ-cells.Protecting pancreatic islets and enhancing insulin secretion has been therapeutic approaches.Mannogalactoglucan is the main type of polysaccharide from natural mushroom,which has potential medicinal prospects.Nevertheless,the antidiabetic property of mannogalactoglucan in T1DM has not been fully elucidated.In this study,we obtained the neutral fraction of alkali-soluble Armillaria mellea polysaccharide(AAMP-N) with the structure of mannogalactoglucan from the fruiting body of A.mellea and investigated the potential therapeutic value of AAMP-N in T1DM.We demonstrated that AAMP-N lowered blood glucose and improved diabetes symptoms in T1DM mice.AAMP-N activated unfolded protein response(UPR) signaling pathway to maintain ER protein folding homeostasis and promote insulin secretion in vivo.Besides that,AAMP-N promoted insulin synthesis via upregulating the expression of transcription factors,increased Ca^(2+) signals to stimulate intracellular insulin secretory vesicle transport via activating calcium/calmodulin-dependent kinase Ⅱ(CamkⅡ) and cAMP/PKA signals,and enhanced insulin secretory vesicle fusion with the plasma membrane via vesicle-associated membrane protein 2(VAMP2).Collectively,these studies demonstrated that the therapeutic potential of AAMP-N on pancreatic islets function,indicating that mannogalactoglucan could be natural nutraceutical used for the treatment of T1DM.展开更多
Objective:To explore the therapeutic effects of the novel wild edible mushroom Astraeus hygrometricus(Pers.)Morgan(A.hygrometricus)on human acute lymphoblastic leukemia cells.Methods:Extensive screening of the antipro...Objective:To explore the therapeutic effects of the novel wild edible mushroom Astraeus hygrometricus(Pers.)Morgan(A.hygrometricus)on human acute lymphoblastic leukemia cells.Methods:Extensive screening of the antiproliferative and chemopreventive potential of different extracts from 5 wild mushrooms,A.hygrometricus,Phallus sp.,Lentinus sp.,Tricholoma sp.,and Serpula sp.was performed against a panel of 6 cancer cell lines and normal cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay.Apoptosis determination,cell cycle profiling,intracellular reactive oxygen species(ROS)and reactive nitrogen species(RNS),and mitochondrial membrane potential were analyzed by flow cytometry.The activity of caspases was measured colorimetrically,and the expression pattern of mitochondrial proteins was analyzed.Results:The methanol extract of A.hygrometricus and MOLT-4 cells were identified as the most potent extract exhibiting antiproliferative activity and most sensitive cell line,respectively.The mushroom extract induced robust selective apoptosis in MOLT-4 cells and arrested cell cycle progression at the G0/G1 stage.The extract disrupted the mitochondrial membrane potential and enhanced ROS production in MOLT-4 cells.The methanol extract induced apoptosis by downregulating the expression of Bcl-2,increasing the expression of Bax,and activating the caspase cascade.Conclusion:The novel wild edible mushroom is a potential repository of biomolecules for the development of antileukemic drugs.展开更多
Hot pot is a popular Chinese dish.It is delicious!You sit at a table with a pot of hot broth.First,the broth has to boil.Then you can cook many different things in it.Many people put seafood and different kinds of mea...Hot pot is a popular Chinese dish.It is delicious!You sit at a table with a pot of hot broth.First,the broth has to boil.Then you can cook many different things in it.Many people put seafood and different kinds of meat in hot pot.Vegetables such as cabbage and corn are delicious in it,too.People also add mushrooms,tofu and noodles to hot pot.Take some cooked food from your hot pot with your chopsticks.Dip it in some sauce and enjoy!展开更多
Metallothionein gene (MT) has been transferred into mushroom protoplasts by electroporation. It is a low molecular weight, cysteine-rich and metal-binding protein. MT can bind metals. Its synthesis is induced by Zn io...Metallothionein gene (MT) has been transferred into mushroom protoplasts by electroporation. It is a low molecular weight, cysteine-rich and metal-binding protein. MT can bind metals. Its synthesis is induced by Zn ion. Thus the expression of MT gene in mushroom can improve the accumulation of Zn in this fungus. This transgenic mushroom, consumed as a kind of vegetable, can supply the necessary Zn to people who are short of the element. When protoplasts were prepared, the concentration (C) of protoplasts is 6.745 x 10(6) /mL. After protoplast electroporation, the transformation rate of protoplasts is 0.01 %. Polymerase chain reaction (PCR) analysis showed that the gene had been integrated into the mushroom chromosome, SDS-PAGE, Western blot analysis indicated that the MT gene had been expressed in the transgenic mushroom. The expressing level, detected by ELISA, is 0.6 % - 0.8 %. Tested for metal resistance, the wild-type mushroom growth was inhibited on die medium containing 1.0 - 1.2 mmoL/L ZnSO4. While the transgenic mushroom was inhibited on the medium containing 1. 5 - 2.0 mmol/L ZnSO4. The mycelium can develop into hymenophore in the medium of rice bran: sawdust = 1: 3, and not in the medium of rice bran: sawdust = 1: 4.展开更多
文摘Academic exchanges,training,and technology transfer under China-Africa cooperation are heralding a sea change in rural Tanzania.Iden Revocatus Stephano,based in Morogoro Region,is a shining example of this youth-driven transition.A graduate of Sokoine University of Agriculture,26-year-old Stephano runs a flourishing mushroom farm-a testament to the growing wave of young Africans reshaping rural economies with global insight and local innovation.
基金National Natural Science Foundation of China (10172063)
文摘Based on Taylor's model and Hawkyard's model, a new simple model for the mushrooming deformation of projectile impacting on a deformable target is installed considering the penetration of the projectile to the deformable target. In the model, the following time-dependent variables are involved in: the extent and the particle velocity in the rigid zone; the extent, the cross-section area and the particle velocity in plastic zone; the velocity and depth of the penetrating of projectile to the target. Solving the set of equations, analytic solution is given. The profiles of deformed projectile and shape parameters for different initial impact velocities are shown. The duration time of deformation increases with increasing the impact velocity. The analytical results by using this model are coincident with experimental result.
文摘Rwandans benefit from Chinese agriculture techniques in both income generation and nutritional value A GROUP of 48 Rwandans were recently trained in mushroom growing,sericulture,soil erosion and upland rice technology,using Chinese knowhow.Mushroom growing technique is part of the
文摘Target occlusion poses a significant challenge in computer vision,particularly in agricultural applications,where occlusion of crops can obscure key features and impair the model’s recognition performance.To address this challenge,a mushroom recognition method was proposed based on an erase module integrated into the EL-DenseNet model.EL-DenseNet,an extension of DenseNet,incorporated an erase attention module designed to enhance sensitivity to visible features.The erase module helped eliminate complex backgrounds and irrelevant information,allowing the mushroom body to be preserved and increasing recognition accuracy in cluttered environments.Considering the difficulty in distinguishing similar mushroom species,label smoothing regularization was employed to mitigate mislabeling errors that commonly arose from human observers.This strategy converted hard labels into soft labels during training,reducing the model’s overreliance on noisy labels and improving its generalization ability.Experimental results showed that the proposed EL-DenseNet,when combined with transfer learning,achieved a recognition accuracy of 96.7%for mushrooms in occluded and complex backgrounds.Compared with the original DenseNet and other classic models,this approach demonstrated superior accuracy and robustness,providing a promising solution for intelligent mushroom recognition.
文摘A substantial body of research has been conducted with the objective of enhancing the understanding of Non-Timber Forest Products (NTFP) in Central Africa. A significant proportion of the studies focus on medicinal plants, in contrast to those that examine food products. Nevertheless, studies on edible wild mushrooms as NTFPs and cultivated mushrooms, on their economic potential and value chains in the Great Lakes Region, remain scarce and largely absent for some NTFPs. In light of the aforementioned considerations, the present study aims to examine the economic potential and value chain of the production sector of wild edible mushrooms (WEM) and cultivated mushrooms (CM) within the Virunga National Park (PNVi) and its surrounding areas in North Kivu, Democratic Republic of the Congo (DRC). To this end, a sample of 432 respondents was selected, distributed equitably among the actors of the Goma-Kitshanga-Mweso value chain, the Goma-Rutshuru chain, the Beni-Mutwanga chain, and the Beni-Mangina chain. A digital questionnaire consisting of open and closed questions was administered, and observations were carried out concurrently. The quantities of mushrooms sold were weighed and photographed. The results demonstrated that, when the eight-month fruiting period of wild and cultivated mushrooms was considered as a single year for all actors within the value chain, a picker could sell 6.1 kg at $2 and earn $12. A seller could sell 16 kg at $4 and earn $64, while a grower could sell 502 kg at $3 and earn $1506. Consequently, the CM grower generates a significantly higher profit than other participants in the value chain, despite the expenses he incurs. Ultimately, the domestication of WEM, highly prized by the local population of the Virunga National Park, has the potential to enhance their household incomes, as evidenced by the findings of this study.
基金supported by RIIM LPDP Grant and BRIN(B-3838/II.7.5/FR.06.00/11/2023)the Research Organization for Nanotechnology and Materials-National Research and Innovation Agency(BRIN)research grand 2025,the postdoctoral program of the National Research and Innovation Agency(BRIN),the Republic of Indonesia Decree Number 140/II/HK/2024.
文摘The increasing demand for renewable energy sources has driven the exploration of innovative materials for biofuel production.This study investigates bio-pellet characteristics derived from several oyster mushroom baglog wastes with varying concentrations of molasses as an adhesive.The process began with sun-drying the baglog waste for three days,followed by oven drying at 80○C for 24 h.Bio-pellets were produced by blending baglog waste with molasses at concentrations of 5%and 10%(w/v),then subsequently fed into a pellet mill.The bio-pellets were left to rest for one hour before analysis.The quality of bio-pellets was determined by evaluating moisture content,ash content,volatile matter,calorific value,combustion rate,density,and compressive strength following SNI 8675:2018 standards.Results indicate that adding molasses as a binder significantly affected the bio-pellet quality.The optimal molasses concentration for balanced performance was found at 5%,providing a lowermoisture content(6.8%),volatile matter(68.42%),and density(1.55 g⋅cm−3).In addition,the bio-pellet has a slightly higher calorific value(approximately 3614 cal⋅g−1),compressive strength(40.68 N⋅mm−2),and ash content(18.59%).All of the parameters for the bio-pellet containing 5%molasses satisfied the standard except for ash content and calorific value.
文摘Traditional taxonomic sorting of samples into recognizable taxonomic units, such as morphospecies or morphotypes, is commonly relied upon in conservation biology and ethnobiological studies. However, understanding the criteria used for traditional nomenclature of fungi, particularly wild edible mushrooms across linguistic groups, remains limited, leading to frequent errors in species recognition. This study seeks to assess how linguistic affiliations influence the local naming of useful wild mushrooms, and is the first of its kind in Benin. In order to understand how local people recognize, classify and name mushrooms that develop in or close to their villages, 2234 respondents from five socio-linguistic groups across three geographical areas were interviewed. Structured and semi-structured interviews were conducted to gather data on the local naming criteria for edible wild mushrooms. Citation scores were recorded for both nomenclature criteria and species, considering variables such as linguistic groups, age, and language. Twenty-two nomenclature criteria were used by local people to name edible wild species. Strong similarity in classification and naming of species was shown in 97% of the languages, while 3% showed differing classification criteria. The Gur, Atlantic, and Mande linguistic groups demonstrated more comprehensive traditional taxonomic and nomenclatural knowledge, sharing six common criteria: texture, taste, size, kingdom (Fungi), form, and substrate. Overall, local populations possess extensive knowledge regarding the diversity of wild edible mushrooms in their environment.
基金supported by the special fund of Jiangsu Province for the transformation of scientific and technological achievements(BA2021062)Jiangsu agricultural science and technology independent innovation fund(CX(22)2007)。
文摘Edible mushroom proteins are the promising ones with the advantages of complete essential amino acid profile and multiple functional activities.To reinforce their applications in functional food development,this study comprehensively evaluated the physicochemical and functional properties of protein isolates from 5 mushroom species,i.e.,Pleurotus eryngii(PEP),Pleurotus ostreatus(POP),Lentinula edodes(LEP),Flammulina velutipes(FVP)and Hypsizygus marmoreus(HMP).Results showed that PEP,LEP,FVP,POP and HMP exhibited better protein solubility(PS),water holding capacity(WHC),emulsification activity index(EAI),and foaming capacity(FC)than those of soybean protein and pea protein isolates(PPI).PEP(51.95%)and POP(49.15%)had a higher amount ofβ-sheet structure.Principal component analysis and correlation analysis showed that the seven proteins could be divided into 3 clusters,and WHC,EAI and FC were significantly positively correlated with PS andβ-sheet.The least gelation concentration of PEP(16%)and FVP(16%)at p H 6.0 and 7.0 was similar to PPI,and PEP showed better hardness,springiness and rheological properties than other proteins gels.Overall,our study showed that 5 edible mushroom proteins possessed excellent functionalities(except for gelling capacity),which provided novel insights on unexploited sources of mushroom proteins used as protein-based foods in the food industry.
基金supported by National Natural Science Foundation of China(No.61862037)Lanzhou Jiaotong University Tianyou Innovation Team Project(No.TY202002)。
文摘To solve the problems of redundant feature information,the insignificant difference in feature representation,and low recognition accuracy of the fine-grained image,based on the ResNeXt50 model,an MSFResNet network model is proposed by fusing multi-scale feature information.Firstly,a multi-scale feature extraction module is designed to obtain multi-scale information on feature images by using different scales of convolution kernels.Meanwhile,the channel attention mechanism is used to increase the global information acquisition of the network.Secondly,the feature images processed by the multi-scale feature extraction module are fused with the deep feature images through short links to guide the full learning of the network,thus reducing the loss of texture details of the deep network feature images,and improving network generalization ability and recognition accuracy.Finally,the validity of the MSFResNet model is verified using public datasets and applied to wild mushroom identification.Experimental results show that compared with ResNeXt50 network model,the accuracy of the MSFResNet model is improved by 6.01%on the FGVC-Aircraft common dataset.It achieves 99.13%classification accuracy on the wild mushroom dataset,which is 0.47%higher than ResNeXt50.Furthermore,the experimental results of the thermal map show that the MSFResNet model significantly reduces the interference of background information,making the network focus on the location of the main body of wild mushroom,which can effectively improve the accuracy of wild mushroom identification.
文摘This study examines the effects of germination substrates incorporating spent mushroom substrate(SMS)and locally sourced organic materials on the growth and vigor of cocoa seedlings.Seven treatments were evaluated using a Completely Randomized Design(CRD),comprising combinations of coconut coir,rice husk,sugarcane bagasse,and biochar.Key findings reveal that treatments combining SMS,sugarcane bagasse,and either coconut coir or biochar(T6 and T7)achieved significantly higher survival rates and Dickson Quality Index(DQI)scores compared to conventional soil(T1).These treatments provided favorable chemical properties,including optimal pH,electrical conductivity,and nutrient content(N,P,K),which are critical for cocoa seedling development.The results suggest that using SMS with agricultural waste as an alternative germination medium can reduce cultivation costs,enhance seedling growth,and contribute to sustainable agricultural practices by repurposing organic waste.This approach not only offers cost-effective benefits for farmers but also mitigates environmental impacts associated with waste disposal.
基金supported by the Key-Area Research and Development Program of Guangdong Province (2021B0707060001)the Program for Scientific Research Start-Up Funds of Guangdong Ocean UniversityChina Postdoctoral Science Foundation (2016T90787)。
文摘The mechanisms of mushroom polysaccharides on immune functions and lipid metabolism of aged mammals have not been fully elucidated.In the present study,after assessing the impacts of one type of Lentinula edodes-derived polysaccharides,named L2,on immune functions and blood lipid profiles,isobaric tags for relative and absolute quantification(iTRAQ)-based proteomic profiling of the small intestinal tissues from aged mice treated with L2 was performed.L2 reversed immune function declines and modulated the lipid metabolism of aged mice evidenced by increased levels of serum TC,HDL-C,and LDL-C,and reduced levels of serum TG.Moreover,a total of 95 differentially regulated proteins(DRPs) were identified,of which75 were up-regulated and 20 were down-regulated.Most of the DRPs were involved in intracellular and extracellular structure organization,and cellular and metabolic regulation.Particularly,approximately 16 and 9 DRPs participated in the regulation of immune functions and lipid metabolism,respectively.Furthermore,protein-protein interaction analysis highlighted that cadherin-1,plectin,cadherin-17,Ras GTPase-activating-like protein IQGAP2,and ezrin might be key proteins in response to L2 treatment.These findings provide new insights into the biological mechanisms of mushroom polysaccharides in anti-aging from a proteomic perspective.
基金the National Natural Science Foundation of China(31972173)the Program for Science&Technology Innovation Talents of Hunan Province(2022SK2100,2021RC4032,and 2019TP1029)the Ministry of Agriculture of the People’s Republic of China(GJFP2021)。
文摘The accumulation of heavy metals in mushrooms has presented a significant risk to human health,underscoring the importance of devising a portable and cost-effective method for detecting heavy metals.Thus,we have developed an electrochemical sensor based on 3-dimensional highly reduced graphene oxide(3D-HRGO)in conjunction with Fe_(3)O_(4)nanoparticles,enabling the simultaneous quantification of Cd^(2+),Pb^(2+),Cu^(2+),and Hg^(2+).The 3D-HRGO/Fe_(3)O_(4)nano-particles material prepared in this study was characterized and confirmed by multiple techniques,then dispersed in a simple and environmental dispersant,consist of 75%ethanol and 0.1%Nafion,and coating on a glass carbon electrode(GCE)to preparing a 3D-HRGO/Fe_(3)O_(4)/GCE sensor.The limit of detection(LOD)of 3D-HRGO/Fe_(3)O_(4)/GCE sensor for Cd^(2+),Pb^(2+),Cu^(2+),and Hg^(2+)in simultaneous detection were 0.2,0.6,0.6,and 0.9μg/L,respectively.The sensor demonstrates exceptional stability,reproducibility,anti-interference,and recovery rate.Furthermore,the electrochemical sensor was employed to detect heavy metals in actual mushrooms and validated through conventional methodologies.This study represents the pioneering utilization of 3D-HRGO/Fe_(3)O_(4)as a foundational material for an electrochemical sensor capable of simultaneous detection of multiple metals,thereby advancing the progress of on-site and expeditious detection techniques.
文摘[Background]Hygrophorus,belonging to Basidiomycota,Agaricomycetes,Agaricales,Hygrophoraceae,is widely distributed all around the world.[Objective]To investigate the species of Hygrophorus and enrich the knowledge about the species diversity of this genus in Shanxi Province.[Methods]We collected fungal specimens,observed and characterized their morphology.After DNA extraction,the internal transcribed spacer(ITS)was sequenced.Phylogenetic trees were constructed based on the maximum likelihood method and Bayesian inference.[Results]Eleven species of Hygrophorus were identified based on the morphological and molecular evidence,including ten known species and a new species.This article described the macroscopic and microscopic morphological characteristics of the new species Hygrophorus brunneodiscoides in detail.The basidiocarp photos of ten known species and a key to the species of Hygrophorus in Shanxi Province were provided.[Conclusion]We identified a new species,H.brunneodiscoides,in China,confirmed 11 species of Hygrophorus in Shanxi Province,and briefed their geographical distribution and associations with hosts according to the field observation.
文摘Novel substrates consisted of different fresh agro-industrial residues,their corresponding and commercial spent mushroom substrates(i.e.SMS deriving from laboratory-scale experiments and SMS deriving from industrial-scale experiments by Green Zin S.A.-SMS GZ)and Pleurotus waste(PW;stipes/mishappen mushrooms)were used in blends for a new cultivation cycle of Pleurotus ostreatus and P.eryngii mushrooms in bags.Their impact on the biochemical properties(intra-cellular polysaccharides-IPSs,protein,lipid,total phenolic compounds-TPCs,individual carbohydrates composition of the IPSs)in the first-and second-flush whole mushrooms,pilei and stipes,as well as the fatty acids composition,the antioxidant activity(in the first-flush mushroom parts)and glucan content of stipes were examined.Both species produced satisfactory IPSs quantities in all substrates(28.69-46.38%,w/w)and significant protein amounts(18.37-26.80%,w/w).The further SMS addition(80%,w/w instead of 40%,w/w)in the cultivation substrates affected positively the mushroom IPSs values,whereas the highest protein content was detected in mushroom’s parts cultivated on substrates consisted of fresh agro-industrial residues.Mushroom’s lipid content was affected differently by the various substrate combinations,with SMS presence resulting in mushrooms with a lower fat content than those produced in substrates with PW addition.Fresh substrates with PW and those with coffee residue were the most favorable for TPCs production.Regarding production flushes,the nutritional value of mushrooms was comparable between them,only a slight increase in TPCs of second-flush carposomes was detected.Glucose was the predominant monosaccharide of the produced IPSs,combined with a significant production of total and β-glucans.SMSs and PW addition had a positive impact on antioxidant activity,too.A higher quantity of lipids,TPCs and significant antioxidant activity were detected in all Pleurotus pilei than stipes,whereas the latter were richer in IPSs.Both pilei and stipes had a significant protein amount.Hence,the data obtained by this study support the positive effect of different types of SMS and mushroom waste on P.ostreatus and P.eryngii nutritional value.
基金supported by the Universiti Malaysia Pahang Al-Sultan Abdullah(UMPSA)(Nos.RDU223010 and PDU223211)。
文摘The oyster mushroom(Pleurotus spp.)is one of the most widely cultivated mushroom species globally.The present study investigated the effect of synbiotics on the growth and quality of Pleurotus ostreatus and Pleurotus pulmonarius.Different synbiotics formulations were applied by spraying mushroom samples daily and measuring their growth parameters,yield,biological efficiency,proximate composition,mineral content,total phenolic content(TPC),and diphenyl-1-picryl-hydrazyl(DPPH)radical scavenging activity.Results demonstrated that the most significant yield of oyster mushrooms was harvested from synbiotics sprayed with inulin and Lactobacillus casei(56.92 g).Likewise,the highest biological efficiency obtained with a similar synbiotic was 12.65%.Combining inulin and L.casei was the most effective method of improving the mushrooms'growth performance and nutrient content in both samples.Furthermore,synbiotics that combined inulin and L.casei resulted in the highest TPC(20.550 mg gallic acid equivalent(GAE)/g dry extract(DE))in white oyster mushrooms(P.ostreatus).In comparison,in grey mushroom(P.pulmonarius)the highest TPC was yielded by L.casei(1.098 mg GAE/g DE)followed by inulin and L.casei(1.079 mg GAE/g DE).The DPPH results indicated that the oyster mushroom could be an efficient antioxidant.The results revealed that applying synbiotics improved the mushrooms'quality by increasing their antioxidant capacity with higher amounts of phenolic compounds and offering better health benefits with the increased levels of mineral elements.Together,these studies demonstrated the potential of using synbiotics as a biofertilizer,which is helpful for mushroom cultivation;therefore,it might solve the challenge of inconsistent quality mushroom growers face.
基金funded by the National Natural Science Foundation of China (32371341,31872674)the Scientific and Technologic Foundation of Jilin Province (20230202050NC)the Fundamental Research Funds for the Central Universities (CGZH202206)。
文摘Type 1 diabetes mellitus(T1DM) lacks insulin secretion due to autoimmune deficiency of pancreaticβ-cells.Protecting pancreatic islets and enhancing insulin secretion has been therapeutic approaches.Mannogalactoglucan is the main type of polysaccharide from natural mushroom,which has potential medicinal prospects.Nevertheless,the antidiabetic property of mannogalactoglucan in T1DM has not been fully elucidated.In this study,we obtained the neutral fraction of alkali-soluble Armillaria mellea polysaccharide(AAMP-N) with the structure of mannogalactoglucan from the fruiting body of A.mellea and investigated the potential therapeutic value of AAMP-N in T1DM.We demonstrated that AAMP-N lowered blood glucose and improved diabetes symptoms in T1DM mice.AAMP-N activated unfolded protein response(UPR) signaling pathway to maintain ER protein folding homeostasis and promote insulin secretion in vivo.Besides that,AAMP-N promoted insulin synthesis via upregulating the expression of transcription factors,increased Ca^(2+) signals to stimulate intracellular insulin secretory vesicle transport via activating calcium/calmodulin-dependent kinase Ⅱ(CamkⅡ) and cAMP/PKA signals,and enhanced insulin secretory vesicle fusion with the plasma membrane via vesicle-associated membrane protein 2(VAMP2).Collectively,these studies demonstrated that the therapeutic potential of AAMP-N on pancreatic islets function,indicating that mannogalactoglucan could be natural nutraceutical used for the treatment of T1DM.
基金indebted toWest Bengal-Department of Science and Technology(1158[Sanc]/ST BT-13015/15/2021-ST SEC)for project funding,University Grants Commission for providing fellowship and contingency to Ribhu Ray,Indian Council of Medical Research for providing fellowship and contingency to Anirban Chouni and UGC-UPE and UGC-CAS program at the Department of Botany,University of Calcutta for financial support.
文摘Objective:To explore the therapeutic effects of the novel wild edible mushroom Astraeus hygrometricus(Pers.)Morgan(A.hygrometricus)on human acute lymphoblastic leukemia cells.Methods:Extensive screening of the antiproliferative and chemopreventive potential of different extracts from 5 wild mushrooms,A.hygrometricus,Phallus sp.,Lentinus sp.,Tricholoma sp.,and Serpula sp.was performed against a panel of 6 cancer cell lines and normal cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay.Apoptosis determination,cell cycle profiling,intracellular reactive oxygen species(ROS)and reactive nitrogen species(RNS),and mitochondrial membrane potential were analyzed by flow cytometry.The activity of caspases was measured colorimetrically,and the expression pattern of mitochondrial proteins was analyzed.Results:The methanol extract of A.hygrometricus and MOLT-4 cells were identified as the most potent extract exhibiting antiproliferative activity and most sensitive cell line,respectively.The mushroom extract induced robust selective apoptosis in MOLT-4 cells and arrested cell cycle progression at the G0/G1 stage.The extract disrupted the mitochondrial membrane potential and enhanced ROS production in MOLT-4 cells.The methanol extract induced apoptosis by downregulating the expression of Bcl-2,increasing the expression of Bax,and activating the caspase cascade.Conclusion:The novel wild edible mushroom is a potential repository of biomolecules for the development of antileukemic drugs.
文摘Hot pot is a popular Chinese dish.It is delicious!You sit at a table with a pot of hot broth.First,the broth has to boil.Then you can cook many different things in it.Many people put seafood and different kinds of meat in hot pot.Vegetables such as cabbage and corn are delicious in it,too.People also add mushrooms,tofu and noodles to hot pot.Take some cooked food from your hot pot with your chopsticks.Dip it in some sauce and enjoy!
文摘Metallothionein gene (MT) has been transferred into mushroom protoplasts by electroporation. It is a low molecular weight, cysteine-rich and metal-binding protein. MT can bind metals. Its synthesis is induced by Zn ion. Thus the expression of MT gene in mushroom can improve the accumulation of Zn in this fungus. This transgenic mushroom, consumed as a kind of vegetable, can supply the necessary Zn to people who are short of the element. When protoplasts were prepared, the concentration (C) of protoplasts is 6.745 x 10(6) /mL. After protoplast electroporation, the transformation rate of protoplasts is 0.01 %. Polymerase chain reaction (PCR) analysis showed that the gene had been integrated into the mushroom chromosome, SDS-PAGE, Western blot analysis indicated that the MT gene had been expressed in the transgenic mushroom. The expressing level, detected by ELISA, is 0.6 % - 0.8 %. Tested for metal resistance, the wild-type mushroom growth was inhibited on die medium containing 1.0 - 1.2 mmoL/L ZnSO4. While the transgenic mushroom was inhibited on the medium containing 1. 5 - 2.0 mmol/L ZnSO4. The mycelium can develop into hymenophore in the medium of rice bran: sawdust = 1: 3, and not in the medium of rice bran: sawdust = 1: 4.
