BACKGROUND The molecular mechanisms involved in micro RNAs(mi RNAs)have been extensively investigated in gastric cancer(GC).However,how mi R-331 regulates GC pathogenesis remains unknown.AIM To illuminate the effect o...BACKGROUND The molecular mechanisms involved in micro RNAs(mi RNAs)have been extensively investigated in gastric cancer(GC).However,how mi R-331 regulates GC pathogenesis remains unknown.AIM To illuminate the effect of mi R-331 on cell metastasis and tumor growth in GC.METHODS The q RT-PCR,CCK8,Transwell,cell adhesion,Western blot,luciferase reporter and xenograft tumor formation assays were applied to explore the regulatory mechanism of mi R-331 in GC.RESULTS Downregulation of mi R-331 associated with poor prognosis was detected in GC.Functionally,mi R-331 suppressed cell proliferation,metastasis and tumor growth in GC.Further,mi R-331 was verified to directly target musashi1(MSI1).In addition,mi R-331 inversely regulated MSI1 expression in GC tissues.Furthermore,upregulation of MSI1 weakened the inhibitory effect of mi R-331 in GC.CONCLUSION mi R-331 inhibited development of GC through targeting MSI1,which may be used as an indicator for the prediction and prognosis of GC.展开更多
文摘BACKGROUND The molecular mechanisms involved in micro RNAs(mi RNAs)have been extensively investigated in gastric cancer(GC).However,how mi R-331 regulates GC pathogenesis remains unknown.AIM To illuminate the effect of mi R-331 on cell metastasis and tumor growth in GC.METHODS The q RT-PCR,CCK8,Transwell,cell adhesion,Western blot,luciferase reporter and xenograft tumor formation assays were applied to explore the regulatory mechanism of mi R-331 in GC.RESULTS Downregulation of mi R-331 associated with poor prognosis was detected in GC.Functionally,mi R-331 suppressed cell proliferation,metastasis and tumor growth in GC.Further,mi R-331 was verified to directly target musashi1(MSI1).In addition,mi R-331 inversely regulated MSI1 expression in GC tissues.Furthermore,upregulation of MSI1 weakened the inhibitory effect of mi R-331 in GC.CONCLUSION mi R-331 inhibited development of GC through targeting MSI1,which may be used as an indicator for the prediction and prognosis of GC.
