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Analysis of NPK in Camellia sinensis, Gliricidia sepium, and Musa acuminata Biomasses for Preparation of an Organic Fertilizers Formula for Young Tea Plants (Musa acuminata) and Studying of Their Nutrient Release Capacity in the Biodegradation Process
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作者 Madushan Dhammika Gunarathna Ashan Sithija Wickramaarachchi +2 位作者 Polegodage Dilushi Sureka Ruwan Kumari Hiti Mudiyanselage Sithara Dilrukshi Wijekoon Deeyagahage Sujeewa Mallik de Silva 《Journal of Agricultural Chemistry and Environment》 2024年第1期115-132,共18页
This study investigates the nitrogen (N), phosphorous (P), and potassium (K) contents in raw biomasses of Camellia sinensis, Gliricidia sepium, and Musa acuminata. Therein, the highest N and P content was seen in Came... This study investigates the nitrogen (N), phosphorous (P), and potassium (K) contents in raw biomasses of Camellia sinensis, Gliricidia sepium, and Musa acuminata. Therein, the highest N and P content was seen in Camellia sinensis 116.80 ± 0.08 mg and 66.00 ± 0.14 mg respectively. The highest K content (106.80 ± 0.04 mg) was observed in Musa acuminata. Next, all three types of plant materials were allowed to decompose in water for 3 weeks, and a sample from each was analyzed for NPK after the 1st, 2nd, and 3rd week during decomposition. A significant increase in the release of N, P, and K by the Camellia sinensis to water (P Musa acuminate were not significantly changed (P > 0.05) over time. The ratio for N:P:K was calculated for raw biomass samples and decomposed samples to find the best fitting N:P:K ratio to apply to young tea plants as organic fertilizers. In addition to that, the microbial insight of these organic compounds was analyzed by observing how microbial population increased with decomposition by the enumeration of the total microbial count. A considerable increment in total microbial count was observed up to 3.28 × 10<sup>6</sup>, 1.21 × 10<sup>10</sup>, 2.18 × 10<sup>8</sup>, and 6.49 × 10<sup>7</sup> CFU/ml for Camellia sinensis, Gliricidia sepium, Musa accuminata (leaves), and Musa accuminata (trunk) respectively. The presence of phosphate solubilizing bacteria (PSB) and nitrogen solubilizing bacteria (NSB) throughout the decomposition period was confirmed by their growth on NBRIP and a modified nutrient medium that was specifically designed for the identification of ammonifiers respectively. Prepared fertilizer samples were applied to young tea plants that were grown in the Mawanella area in Sri Lanka (7°15'12.42"N 80°26'47.62"E) and according to the results, it is clear that fertilizer mixture 1 (N:P:K, 10:5:10, tea dust + Gliricidia + banana trunk) and fertilizer mixture 2 (N:P:K, 10:5:10, tea dust + Gliricidia + banana leaves) has the potential to increase the growth of young tea plants. 展开更多
关键词 Camellia sinensis musa accuminata Gliricidia sepium Phosphate Solubilizing Bacteria Nitrogen Solubilizing Bacteria
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香蕉Actin1启动子的分离及启动活性的分析 被引量:3
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作者 徐碧玉 刘歌 金志强 《热带作物学报》 CSCD 2005年第1期34-37,共4页
从香蕉基因组DNA中分离香蕉Actin1启动子序列,序列测定结果表明,该片段1253bp,包含完整的G-box和TATAbox,5'非翻译区和其下游的内含子,与文献报道序列的同源性达97.53%。以全长序列取代植物表达载体pCAMBIA3300上的35S启动子序列构... 从香蕉基因组DNA中分离香蕉Actin1启动子序列,序列测定结果表明,该片段1253bp,包含完整的G-box和TATAbox,5'非翻译区和其下游的内含子,与文献报道序列的同源性达97.53%。以全长序列取代植物表达载体pCAMBIA3300上的35S启动子序列构建成瞬间表达载体pCAMBIA-Act1,以内含子序列HindⅢ和XbaⅠ双酶切片段(584bp)取代植物表达载体pBI121上的35S启动子,构建成瞬间表达载体pBI121-Act1-1。采用基因枪法对香蕉根、叶和果实的薄片进行转化,转化材料经培养3h,采用分光光度法测定各组织GUS的活性。结果表明,Actin1启动子在所转化的3种组织中均可启动报告基因的表达,表达活性与35S启动子接近,具有组成型表达的特点。内含子部分序列(584bp)也具有启动活性,但启动活性较低。 展开更多
关键词 香蕉 Actin1 启动子 分离 启动活性 肌动蛋白基因 瞬间表达 基因枪法
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