Determining the optical properties of media remains an important part of scientific research. Knowledge of these optical properties, particularly absorption and diffusion coefficients, has direct applications in biome...Determining the optical properties of media remains an important part of scientific research. Knowledge of these optical properties, particularly absorption and diffusion coefficients, has direct applications in biomedical therapeutic diagnostics. The determination of these coefficients was previously reserved for optically dilute media. Recently, a technique called Structured Laser Illumination Planar Imaging (SLIPI) has been developed for measuring extinction coefficients in dense media. For such a medium and technique, no study has reported the determination of absorption and scattering coefficients. In this study, we have developed a simple calculation method based on the combination of Kubelka-Munk relations and extinction, both functions of the medium’s absorption and diffusion coefficients. The equations thus developed enable absorption and diffusion coefficients to be easily calculated from extinction coefficient measurements alone, using the SLIPI technique. The analysis method thus developed was applied to ten (10) milk solutions of different concentrations considered to be predominantly diffusive, and to ten (10) coffee solutions of different concentrations considered to be predominantly absorbent. The coefficient values obtained have been analysed and compared to the literature ones and they would be satisfactory.展开更多
文摘Determining the optical properties of media remains an important part of scientific research. Knowledge of these optical properties, particularly absorption and diffusion coefficients, has direct applications in biomedical therapeutic diagnostics. The determination of these coefficients was previously reserved for optically dilute media. Recently, a technique called Structured Laser Illumination Planar Imaging (SLIPI) has been developed for measuring extinction coefficients in dense media. For such a medium and technique, no study has reported the determination of absorption and scattering coefficients. In this study, we have developed a simple calculation method based on the combination of Kubelka-Munk relations and extinction, both functions of the medium’s absorption and diffusion coefficients. The equations thus developed enable absorption and diffusion coefficients to be easily calculated from extinction coefficient measurements alone, using the SLIPI technique. The analysis method thus developed was applied to ten (10) milk solutions of different concentrations considered to be predominantly diffusive, and to ten (10) coffee solutions of different concentrations considered to be predominantly absorbent. The coefficient values obtained have been analysed and compared to the literature ones and they would be satisfactory.
