BACKGROUND Metastasis is the main reason leading to death in colorectal cancer(CRC)and about 25%of CRC patients developed metastasis when first diagnosed.Thus,unveiling biomarkers of CRC metastasis is of great signifi...BACKGROUND Metastasis is the main reason leading to death in colorectal cancer(CRC)and about 25%of CRC patients developed metastasis when first diagnosed.Thus,unveiling biomarkers of CRC metastasis is of great significance.AIM To reveal biomarkers of CRC metastasis.METHODS Weighted gene co-expression network analysis was conducted to identify metastatic biomarkers in CRC through a systematic analysis of the GSE29621 dataset.Comprehensive validation was performed subsequently using publicly available datasets from The Cancer Genome Atlas and Gene Expression Omnibus and supplemented with experimental verification in CRC cell lines.Moreover,the identified hub gene charged multivesicular body protein 7(CHMP7)was further subjected to clinical correlation analysis via Kaplan-Meier survival curves and Gene Set Enrichment Analysis to assess its prognostic significance and potential mechanistic involvement in CRC progression.RESULTS CHMP7 was identified as a key metastatic biomarker of CRC which displayed lower expression in CRC tissues,especially in CRC patients with metastasis and CRC cell lines with high metastasis potential.The expression of CHMP7 was significantly correlated with normal,metastatic tumor,pathologic stage,and lymphatic invasion(P<0.05).CRC patients with higher expression of CHMP7 exhibited better overall survival.Besides,Gene Set Enrichment Analysis results showed that CHMP7 might be involved in metastatic related pathways.CONCLUSION Our results indicate that CHMP7 might be a prognostic biomarker correlated with CRC metastasis.展开更多
A combinational therapeutic system that simultaneously administrates various pathways is preferred for anti-cancer treatment.In the present study,we successfully constructed a co-delivery system,multivesicular liposom...A combinational therapeutic system that simultaneously administrates various pathways is preferred for anti-cancer treatment.In the present study,we successfully constructed a co-delivery system,multivesicular liposomes(MVLs)co-encapsulating doxorubicin(DOX)and luminespib(AUY922).A simple and accurate dual-wavelength spectrophotometric method was established for the determination of DOX and AUY922 in liposomal formulation.MVL-loading drugs were prepared by a multi-emulsion solvent evaporation method,which exhibited excellent physicochemical properties,such as particle size of 3–8μm and high entrapment efficiency above 95%for DOX and 73%for AUY922.The synergetic cytotoxic effect for these two drugs was evaluated in MDA-MB-231 cells.The in vitro antitumor studies demonstrated the superior anti-proliferation activity of DOX and AUY922 with a combination index of 0.43,indicating a great synergistic effect.The experimental data suggested that combinational use of DOX and AUY922 within liposomes could be an effective way to develop efficient treatments of cancers.展开更多
Colorectal cancer(CRC)is a leading cause of cancer-related mortality,primarily due to tumor metastasis.A recent study in the World Journal of Gastrointestinal Oncology identifies charged multivesicular body protein 7(...Colorectal cancer(CRC)is a leading cause of cancer-related mortality,primarily due to tumor metastasis.A recent study in the World Journal of Gastrointestinal Oncology identifies charged multivesicular body protein 7(CHMP7)as a key prognostic factor in CRC.The study showed that CHMP7 expression is significantly lower in patients with CRC with metastasis and in highly metastatic cell lines,correlating with clinical factors like normal tissue,metastatic tumors,pathologic stage,and lymphatic invasion.Higher CHMP7 expression was linked to improved overall survival,highlighting its potential as a predictive biomarker.Gene Set Enrichment Analysis also suggests the role of CHMP7 in metastasisrelated pathways,paving the way for further mechanistic studies.This finding challenges current CRC management strategies and calls for larger,prospective studies to validate the role of CHMP7.As a potential target for novel diagnostics and therapies,CHMP7 could advance personalized medicine in CRC,bridging molecular insights with clinical outcomes to improve patient prognosis.展开更多
Plant prevacuolar compartments （PVCs）, or multivesicular bodies （MVBs）, are single membrane-bound organelles that play important roles in mediating protein trafficking to vacuoles in the secretory pathway. PVC/MVB...Plant prevacuolar compartments （PVCs）, or multivesicular bodies （MVBs）, are single membrane-bound organelles that play important roles in mediating protein trafficking to vacuoles in the secretory pathway. PVC/MVB also serves as a late endosome in the endocytic pathway in plants. Since the plant PVC was iden- tified as an MVB more than 10 years ago,-great progress has been made toward the understanding of PVC/ MVB function and biogenesis in plants. In this review, we first summarize previous research into the iden- tification and characterization of plant PVCs/MVBs, and then highlight recent advances on the mechanisms underlying intraluminal vesicle formation and maturation of plant PVCs/MVBs. In addition, we discuss the possible crosstalk that appears to occur between PVCs/MVBs and autophagosomes during autophagy in plants. Finally, we list some open questions and present future perspectives in this field.展开更多
基金Supported by the National Natural Science Foundation of China,No.82260715the Middle-Aged and Young Teachers in Colleges and Universities in Guangxi Basic Ability Promotion Project,No.2024KY0302+2 种基金Guangxi Collaborative Innovation Center for Research on Functional Ingredients of Agricultural Residues,No.CICAR2016-P6the Grant of Research Project on High-Level Talents of Youjiang Medical College for Nationalities,No.YY2021SK002Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province,No.202300011.
文摘BACKGROUND Metastasis is the main reason leading to death in colorectal cancer(CRC)and about 25%of CRC patients developed metastasis when first diagnosed.Thus,unveiling biomarkers of CRC metastasis is of great significance.AIM To reveal biomarkers of CRC metastasis.METHODS Weighted gene co-expression network analysis was conducted to identify metastatic biomarkers in CRC through a systematic analysis of the GSE29621 dataset.Comprehensive validation was performed subsequently using publicly available datasets from The Cancer Genome Atlas and Gene Expression Omnibus and supplemented with experimental verification in CRC cell lines.Moreover,the identified hub gene charged multivesicular body protein 7(CHMP7)was further subjected to clinical correlation analysis via Kaplan-Meier survival curves and Gene Set Enrichment Analysis to assess its prognostic significance and potential mechanistic involvement in CRC progression.RESULTS CHMP7 was identified as a key metastatic biomarker of CRC which displayed lower expression in CRC tissues,especially in CRC patients with metastasis and CRC cell lines with high metastasis potential.The expression of CHMP7 was significantly correlated with normal,metastatic tumor,pathologic stage,and lymphatic invasion(P<0.05).CRC patients with higher expression of CHMP7 exhibited better overall survival.Besides,Gene Set Enrichment Analysis results showed that CHMP7 might be involved in metastatic related pathways.CONCLUSION Our results indicate that CHMP7 might be a prognostic biomarker correlated with CRC metastasis.
基金School of Pharmaceutical Sciences,Peking University Health Science Center,Beijing 100191,China。
文摘A combinational therapeutic system that simultaneously administrates various pathways is preferred for anti-cancer treatment.In the present study,we successfully constructed a co-delivery system,multivesicular liposomes(MVLs)co-encapsulating doxorubicin(DOX)and luminespib(AUY922).A simple and accurate dual-wavelength spectrophotometric method was established for the determination of DOX and AUY922 in liposomal formulation.MVL-loading drugs were prepared by a multi-emulsion solvent evaporation method,which exhibited excellent physicochemical properties,such as particle size of 3–8μm and high entrapment efficiency above 95%for DOX and 73%for AUY922.The synergetic cytotoxic effect for these two drugs was evaluated in MDA-MB-231 cells.The in vitro antitumor studies demonstrated the superior anti-proliferation activity of DOX and AUY922 with a combination index of 0.43,indicating a great synergistic effect.The experimental data suggested that combinational use of DOX and AUY922 within liposomes could be an effective way to develop efficient treatments of cancers.
文摘Colorectal cancer(CRC)is a leading cause of cancer-related mortality,primarily due to tumor metastasis.A recent study in the World Journal of Gastrointestinal Oncology identifies charged multivesicular body protein 7(CHMP7)as a key prognostic factor in CRC.The study showed that CHMP7 expression is significantly lower in patients with CRC with metastasis and in highly metastatic cell lines,correlating with clinical factors like normal tissue,metastatic tumors,pathologic stage,and lymphatic invasion.Higher CHMP7 expression was linked to improved overall survival,highlighting its potential as a predictive biomarker.Gene Set Enrichment Analysis also suggests the role of CHMP7 in metastasisrelated pathways,paving the way for further mechanistic studies.This finding challenges current CRC management strategies and calls for larger,prospective studies to validate the role of CHMP7.As a potential target for novel diagnostics and therapies,CHMP7 could advance personalized medicine in CRC,bridging molecular insights with clinical outcomes to improve patient prognosis.
文摘Plant prevacuolar compartments （PVCs）, or multivesicular bodies （MVBs）, are single membrane-bound organelles that play important roles in mediating protein trafficking to vacuoles in the secretory pathway. PVC/MVB also serves as a late endosome in the endocytic pathway in plants. Since the plant PVC was iden- tified as an MVB more than 10 years ago,-great progress has been made toward the understanding of PVC/ MVB function and biogenesis in plants. In this review, we first summarize previous research into the iden- tification and characterization of plant PVCs/MVBs, and then highlight recent advances on the mechanisms underlying intraluminal vesicle formation and maturation of plant PVCs/MVBs. In addition, we discuss the possible crosstalk that appears to occur between PVCs/MVBs and autophagosomes during autophagy in plants. Finally, we list some open questions and present future perspectives in this field.
