Pamiparib is a potent and selective oral poly(adenosine diphosphate(ADP)-ribose)-polymerase(PARP)1/2inhibitor(PARPi).Pamiparib has good bioavailability and shows greater cytotoxic potency and similar DNA-trapping capa...Pamiparib is a potent and selective oral poly(adenosine diphosphate(ADP)-ribose)-polymerase(PARP)1/2inhibitor(PARPi).Pamiparib has good bioavailability and shows greater cytotoxic potency and similar DNA-trapping capacity compared to olaparib.It is not affected by adenosine triphosphate(ATP)-binding cassette transporters.展开更多
BACKGROUND Congenital renal arteriovenous fistula(RAVF)is a clinically rare condition and frequently missed and misdiagnosed.Multimodal imaging techniques can pro-vide more detailed diagnostic information to help phys...BACKGROUND Congenital renal arteriovenous fistula(RAVF)is a clinically rare condition and frequently missed and misdiagnosed.Multimodal imaging techniques can pro-vide more detailed diagnostic information to help physicians more accurately diagnose and treat diseases.Combining imaging methods to diagnose RAVF has rarely been reported.CASE SUMMARY A 69-year-old female patient presented with gross hematuria that had persisted for 10 days.The patient underwent ultrasound examinations of the kidneys and renal blood vessels,enhanced computed tomography,three-dimensional com-puted tomography angiography,and digital subtraction angiography of the renal arteries.These revealed dilatation of the left renal vein and abnormal shunting between the left renal artery and vein.The patient was diagnosed with a left RAVF using combined multimodal imaging techniques.The patient was treated with left renal artery embolization immediately after renal arteriography.Hema-turia resolved following the left renal artery embolization without serious bleeding or other complications.The patient made a full recovery after one year of postoperative follow-up.CONCLUSION Multimodal imaging techniques complement each other when diagnosing RAVF,providing detailed diagnostic information that can aid in accurate diagnosis and treatment.In addition,this case reminds the sonographer to pay more attention to the color doppler flow imaging and blood flow spectrum when examining the kidney,so as to avoid misdiagnosis of renal cystic lesions as renal cysts and missed diagnosis of RAVF.展开更多
AIM:To describe the multimodal imaging features,treatment,and outcomes of patients diagnosed with adultonset Coats disease.METHODS:This retrospective study included patients first diagnosed with Coats disease at≥18 y...AIM:To describe the multimodal imaging features,treatment,and outcomes of patients diagnosed with adultonset Coats disease.METHODS:This retrospective study included patients first diagnosed with Coats disease at≥18 years of age between September 2017 and September 2021.Some patients received anti-vascular endothelial growth factor(VEGF)therapy(conbercept,0.5 mg)as the initial treatment,which was combined with laser photocoagulation as needed.All the patients underwent best corrected visual acuity(BCVA)and intraocular pressure examinations,fundus color photography,spontaneous fluorescence tests,fundus fluorescein angiography,optical coherence tomography(OCT),OCT angiography,and other examinations.BCVA alterations and multimodal image findings in the affected eyes following treatment were compared and the prognostic factors were analyzed.RESULTS:The study included 15 patients who were aged 24-72(57.33±12.61)y at presentation.Systemic hypertension was the most common associated systemic condition,occurring in 13(86.7%)patients.Baseline BCVA ranged from 2.0 to 5.0(4.0±1.1),which showed improvement following treatment(4.2±1.0).Multimodal imaging revealed retinal telangiectasis in 13 patients(86.7%),patchy hemorrhage in 5 patients(33.3%),and stage 2B disease(Shield’s staging criteria)in 11 patients(73.3%).OCT revealed that the baseline central macular thickness(CMT)ranged from 129 to 964μm(473.0±230.1μm),with 13 patients(86.7%)exhibiting a baseline CMT exceeding 250μm.Furthermore,8 patients(53.3%)presented with an epiretinal membrane at baseline or during follow-up.Hyper-reflective scars were observed on OCT in five patients(33.3%)with poor visual prognosis.Vision deteriorated in one patient who did not receive treatment.Final vision was stable in three patients who received laser treatment,whereas improvement was observed in one of two patients who received anti-VEGF therapy alone.In addition,8 of 9 patients(88.9%)who received laser treatment and conbercept exhibited stable or improved BCVA.CONCLUSION:Multimodal imaging can help diagnose adult-onset Coats disease.Anti-VEGF treatment combined with laser therapy can be an option for improving or maintaining BCVA and resolving macular edema.The final visual outcome depends on macular involvement and the disease stage.展开更多
BACKGROUND Giant cell tumor of bone is a locally aggressive and rarely metastasizing tumor,and also a potential malignant tumor that may develop into a primary malignant giant cell tumor.AIM To evaluate the role of mu...BACKGROUND Giant cell tumor of bone is a locally aggressive and rarely metastasizing tumor,and also a potential malignant tumor that may develop into a primary malignant giant cell tumor.AIM To evaluate the role of multimodal imaging in the diagnosis of giant cell tumors of bone.METHODS The data of 32 patients with giant cell tumor of bone confirmed by core-needle biopsy or surgical pathology at our hospital between March 2018 and March 2023 were retrospectively selected.All the patients with giant cell tumors of the bone were examined by X-ray,computed tomography(CT)and magnetic resonance imaging(MRI),and 7 of them were examined by positron emission tomography(PET)-CT.RESULTS X-ray imaging can provide overall information on giant cell tumor lesions.CT and MRI can reveal the characteristics of the internal structure of the tumor as well as the adjacent relationships of the tumor,and these methods have unique advantages for diagnosing tumors and determining the scope of surgery.PET-CT can detect small lesions and is highly valuable for identifying benign and malignant tumors to aid in the early diagnosis of metastasis.CONCLUSION Multimodal imaging plays an important role in the diagnosis of giant cell tumor of bone and can provide a reference for the treatment of giant cell tumors.展开更多
BACKGROUND The rare co-occurrence of oligodendroglioma and arteriovenous malformation(AVM)in the same intracranial location.CASE SUMMARY In a 61-year-old man presenting with progressive headaches,is described in this ...BACKGROUND The rare co-occurrence of oligodendroglioma and arteriovenous malformation(AVM)in the same intracranial location.CASE SUMMARY In a 61-year-old man presenting with progressive headaches,is described in this case study.Preoperative multimodal imaging techniques(computed tomography,magnetic resonance imaging,magnetic resonance spectroscopy,digital subtraction angiography,and computed tomography angiography)were employed to detect hemorrhage,cystic and solid lesions,and arteriovenous shunting in the right temporal lobe.The patient underwent right temporal craniotomy for lesion removal,and postoperative pathological analysis confirmed the presence of oligodendroglioma(World Health Organization grade II,not otherwise specified)and AVM.CONCLUSION The preoperative utilization of multimodal imaging examination can help clinicians reduce the likelihood of misdiagnosis or oversight of these conditions,and provides important information for subsequent treatment.This case supports the feasibility of craniotomy for the removal of glioma with AVM.展开更多
AIM:To describe the clinical characteristics of eyes using multimodal imaging features with acute macular neuroretinopathy(AMN)lesions following severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection.MET...AIM:To describe the clinical characteristics of eyes using multimodal imaging features with acute macular neuroretinopathy(AMN)lesions following severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection.METHODS:Retrospective case series study.From December 18,2022 to February 14,2023,previously healthy cases within 1-week infection with SARS-CoV-2 and examined at Tianjin Eye Hospital to confirm the diagnosis of AMN were included in the study.Totally 5 males and 9 females[mean age:29.93±10.32(16-49)y]were presented for reduced vision,with or without blurred vision.All patients underwent best corrected visual acuity(BCVA),intraocular pressure,slit lamp microscopy,indirect fundoscopy.Simultaneously,multimodal imagings fundus photography(45°or 200°field of view)was performed in 7 cases(14 eyes).Near infrared(NIR)fundus photography was performed in 9 cases(18 eyes),optical coherence tomography(OCT)in 5 cases(10 eyes),optical coherence tomography angiography(OCTA)in 9 cases(18 eyes),and fundus fluorescence angiography(FFA)in 3 cases(6 eyes).Visual field was performed in 1 case(2 eyes).RESULTS:Multimodal imaging findings data from 14 patients with AMN were reviewed.All eyes demonstrated different extent hyperreflective lesions at the level of the inner nuclear layer and/or outer plexus layer on OCT or OCTA.Fundus photography(45°or 200°field of view)showed irregular hypo-reflective lesion around the fovea in 7 cases(14 eyes).OCTA demonstrated that the superficial retinal capillary plexus(SCP)vascular density,deep capillary plexus(DCP)vascular density and choriocapillaris(CC)vascular density was reduced in 9 case(18 eyes).Among the follow-up cases(2 cases),vascular density increased in 1 case with elevated BCVA;another case has vascular density decrease in one eye and basically unchanged in other eye.En face images of the ellipsoidal zone and interdigitation zone injury showed a low wedge-shaped reflection contour appearance.NIR image mainly show the absence of the outer retinal interdigitation zone in AMN.No abnormal fluorescence was observed in FFA.Corresponding partial defect of the visual field were visualized via perimeter in one case.CONCLUSION:The morbidity of SARS-CoV-2 infection with AMN is increased.Ophthalmologists should be aware of the possible,albeit rare,AMN after SARS-CoV-2 infection and focus on multimodal imaging features.OCT,OCTA,and infrared fundus phase are proved to be valuable tools for detection of AMN in patients with SARS-CoV-2.展开更多
Multimodal imaging,including augmented or mixed reality,transforms the physicians’interaction with clinical imaging,allowing more accurate data interpretation,better spatial resolution,and depth perception of the pat...Multimodal imaging,including augmented or mixed reality,transforms the physicians’interaction with clinical imaging,allowing more accurate data interpretation,better spatial resolution,and depth perception of the patient’s anatomy.We successfully overlay 3D holographic visualization to magnetic resonance imaging images for preoperative decision making of a complex case of cardiac tumour in a 7-year-old girl.展开更多
Developing low toxicity and multifunctional theranostic nanoplatform is the key for precise cancer diagnosis and treatment.Herein,an inorganic-organic hybrid nanocomposite is designed by modifying zirconium dioxide(Zr...Developing low toxicity and multifunctional theranostic nanoplatform is the key for precise cancer diagnosis and treatment.Herein,an inorganic-organic hybrid nanocomposite is designed by modifying zirconium dioxide(ZrO_(2)) with polydopamine(PDA) followed by doping Mn^(2+) ions and functionalizing with Tween 20(Tween-ZrO_(2)@PDA-Mn2+) for multimodal imaging and chemo-photothermal combination therapy.The as-prepared nanocomposite exhibits good biocompatibility in vitro and in vivo.Specifically,it can be employed as a multifunctional platform not only for computed tomography(CT)imaging and T1-weighted magnetic resonance(MR) imaging,but also for efficient chemotherapeutic drug doxorubicin hydrochloride(DOX) loading.Importantly,because of the pronounced photothermal conversion performance and controllable DOX release ability triggered by the near-infrared(NIR)irradiation and acidic pH,the synergistic effect between photothermal the rapy and chemotherapy results in an enhanced cancer treatment efficacy in vivo.Our work provides a high-performance inorganicorganic hybrid nanotheranostic platform for chemo-photothermal cancer therapy guided by CT and MR imaging.展开更多
Photoacoustic imaging(PAI)is a hybrid imaging method based on photoacoustic(PA)effects,which is able to capture the structure,function,and molecular information of biological tissues with high resolution.To date,thera...Photoacoustic imaging(PAI)is a hybrid imaging method based on photoacoustic(PA)effects,which is able to capture the structure,function,and molecular information of biological tissues with high resolution.To date,therapeutic techniques under the guidance of PAI have provided new strategies for accurate diagnosis and precise treatment of tumors.In particular,conjugated polymer nanoparticles have been extensively inspected for PA-based cancer theranostics largely due to their superior optical properties such as tunable spectrum and large absorption coefficient and their good biocompatibility,and abundant functional groups.This mini-review mainly focuses on the recent advances toward the development of novel conjugated polymer nanoparticles for PA-based multimodal imaging and cancer photothermal therapy.展开更多
AIM： To describe retinal findings of various imaging modalities in acute retinal ischemia. METHODS： Fluorescein angiography（FA）, spectral domain optical coherence tomography（SD-OCT）, OCTangiography（OCT-A） and ...AIM： To describe retinal findings of various imaging modalities in acute retinal ischemia. METHODS： Fluorescein angiography（FA）, spectral domain optical coherence tomography（SD-OCT）, OCTangiography（OCT-A） and fundus autofluorescence（FAF） images of 13 patients（mean age 64y, range 28-86y） with acute retinal ischemia were evaluated. Six suffered from branch arterial occlusion, 2 had a central retinal artery occlusion, 2 had a combined arteriovenous occlusions, 1 patient had a retrobulbar arterial compression by an orbital haemangioma and 2 patients showed an ocular ischemic syndrome.RESULTS： All patients showed increased reflectivity and thickening of the ischemic retinal tissue. In 10 out of 13 patients SD-OCT revealed an additional highly reflective band located within or above the outer plexiform layer. Morphological characteristics were a decreasing intensity with distance from the fovea, partially segmental occurrence and manifestation limited in time. OCT-A showed a loss of flow signal in the superficial and deep capillary plexus at the affected areas. Reduced flow signal was detected underneath the regions with retinal edema. FAF showed areas of altered signal intensity at the posterior pole. The regions of decreased FAF signal corresponded to peri-venous regions. CONCLUSION： Multimodal imaging modalities in retinal ischemia yield characteristic findings and valuable diagnostic information. Conventional OCT identifies hyperreflectivity and thickening and a mid-retinal hyperreflective band is frequently observed. OCT-A examination reveals demarcation of the ischemic retinal area on the vascular level. FAF shows decreased fluorescence signal in areas of retinal edema often corresponding to peri-venous regions.展开更多
The phenotypes of the adenine-to-guanine transition at position 3243 of mitochondrial DNA(m.3243A>G)are highly variable,with different symptoms observed in different patients.These include mitochondrial encephalomy...The phenotypes of the adenine-to-guanine transition at position 3243 of mitochondrial DNA(m.3243A>G)are highly variable,with different symptoms observed in different patients.These include mitochondrial encephalomyopathy,lactic acidosis,and stroke-like episodes(MELAS);maternally inherited diabetes and deafness syndrome(MIDD);other syndromic conditions;or non-syndromic mitochondrial disorders.Renal involvement associated with this mutation generally manifests as subnephrotic proteinuria,progressive deterioration of kidney function,and increased morbidity.The retinopathies linked to the m.3243A>G mutation have heterogeneous presentations,characterized by variable degrees of retinal pigment epithelium(RPE)atrophy and hyperpigmentation at the posterior pole.As a severe phenotype of the m.3243A>G mutation,MELAS combined with focal and segmental glomerulosclerosis(FSGS)is rare.We herein firstly reported in detail the ophthalmic manifestations of a patient with this condition.Additionally,we reviewed the literature on fundus,ophthalmic electrophysiology,and optical coherence tomography(OCT)findings related to the m.3243A>G mutation.展开更多
Osteosarcoma is the most common primary bone tumor with high malignancy.It is particularly necessary to achieve rapid and accurate diagnosis in its intraoperative examination and early diagnosis.Accordingly,the multim...Osteosarcoma is the most common primary bone tumor with high malignancy.It is particularly necessary to achieve rapid and accurate diagnosis in its intraoperative examination and early diagnosis.Accordingly,the multimodal microscopic imaging diagnosis system constructed by bright field,spontaneous fluorescence and polarized light microscopic imaging was used to study the pathological mechanism of osteosarcoma from the tissue microenvironment level and achieve rapid and accurate diagnosis.First,the multimodal microscopic images of normal and osteosarcoma tissue slices were collected to characterize the overall morphology of the tissue microenvironment of the samples,the arrangement structure of collagen fibers and the content and distribution of endogenous fluorescent substances.Second,based on the correlation and complementarity of the feature information contained in the three single-mode images,combined with convolutional neural network(CNN)and image fusion methods,a multimodal intelligent diagnosis model was constructed to effectively improve the information utilization and diagnosis accuracy.The accuracy and true positivity of the multimodal diagnostic model were significantly improved to 0.8495 and 0.9412,respectively,compared to those of the single-modal models.Besides,the difference of tissue microenvironments before and after cancerization can be used as a basis for cancer diagnosis,and the information extraction and intelligent diagnosis of osteosarcoma tissue can be achieved by using multimodal microscopic imaging technology combined with deep learning,which significantly promoted the application of tissue microenvironment in pathological examination.This diagnostic system relies on its advantages of simple operation,high efficiency and accuracy and high cost-effectiveness,and has enormous clinical application potential and research significance.展开更多
Near-infrared Ⅱ(NIR-Ⅱ)fluorescent nanoparticles(NPs)based on aggregation-induced emission(AIE)have attracted significant attention due to theirmultimodal imaging capabilities aswell as the combined photothermal and ...Near-infrared Ⅱ(NIR-Ⅱ)fluorescent nanoparticles(NPs)based on aggregation-induced emission(AIE)have attracted significant attention due to theirmultimodal imaging capabilities aswell as the combined photothermal and photodynamic therapeutic effects in cancer therapy.Reported herein is the rational designed AIE molecule(BPT),via incorporating phenothiazine units with strong electron-donating and reactive oxygen species(ROS)generation capabilities into the classical AIE scaffold tetraphenylethylene,further coupled with a strong electron-acceptor named benzo[1,2-c:4,5-c’]bis[1,2,5]thiadiazole.The BPT NPs exhibited maximum NIR-Ⅱ fluorescence emission at 1083 nm,a fluorescence quantum yield of 1.53%,photothermal conversion efficiency of 63%,and photoacoustic imaging capabilities,alongside considerable type I ROS generation ability.Additionally,when a kind of nitric oxide(NO)donor named O_(2)-(2,4-dinitrophenyl)1-[(4-ethoxycarbonyl)piperazin-1-yl]diazen-1-ium-1,2-diolate(JSK)was incorporated,the corresponding JSK-BPT NPs could generate O_(2)^(−),NO,and peroxynitrite to induce phototoxicity.By applying it to the 4T1 breast tumor model,JSK-BPT NPs achieved high-quality multimodal imaging of the vasculature and tumor regions in mice.Under the multimodal imaging guidance,the 4T1 tumor could be ablated completely after a single dose of JSK-BPT NPs and under the irradiation of an 808 nm laser.展开更多
BACKGROUND Rectal cancer is one of the common digestive system malignant tumors around the world.Its early diagnosis and staging are crucial for rectal cancer treatment and prognosis.In recent years,tumor markers have...BACKGROUND Rectal cancer is one of the common digestive system malignant tumors around the world.Its early diagnosis and staging are crucial for rectal cancer treatment and prognosis.In recent years,tumor markers have gradually received attention in early screening,treatment monitoring and prognostic evaluation of cancer,but their predictive role in rectal cancer staging and differentiation is still unclear.AIM To assess the prognostic value of tumor markers alpha-fetoprotein(AFP)cancer antigen 72-4(CA72-4),carbohydrate antigen 19-9(CA19-9),and carcinoembryonic antigen(CEA),alongside multimodal magnetic resonance imaging(MRI),for staging and differentiating rectal cancer in patients.METHODS This study retrospectively analyzed 167 patients with rectal cancer who were treated at our institution from January 2020 to December 2024.Each patient underwent serological testing and multimodal MRI for diagnosis.Histopathological examination after surgical resection or imaging based on follow-up was used as the gold standard.According to the T stage and differentiation degree,patients were divided into low stage group(T1-T2)and high stage group(T3-T4).In addition,they were divided into low-differentiation groups and high-differentiation groups according to their differentiation degree.We compared the accuracy,sensitivity and specificity of tumor marker levels and MRI in rectal cancer stage and differentiation.RESULTS The study's findings indicate that in the context of rectal cancer T staging,there is substantial concordance between MRI and clinicopathological assessments,with a Kappa coefficient of 0.789(P<0.001).Similarly,for various degrees of tumor differentiation,MRI and clinicopathological evaluations demonstrated substantial agreement,with a Kappa coefficient of 0.651(P<0.001).Notably,the concentrations of tumor markers CA19-9,CA72-4,CEA,and AFP were significantly elevated in the T3-T4 stage compared to the T1-T2 stage.Furthermore,these markers were significantly higher in the low-differentiation group compared to the high-differentiation group(P<0.05).The combined use of tumor markers and MRI for preoperative T staging of rectal cancer yielded a diagnostic sensitivity of 93.7%and a specificity of 94.6%,as evidenced by the receiver operating characteristic analysis,with an area under the curve of 0.947.For tumor differentiation,the diagnostic sensitivity and specificity were 93.6%and 97.1%,respectively,with an area under the curve of 0.978(95%confidence interval:0.946-1.000),surpassing the accuracy of individual detection methods.CONCLUSION The CA19-9,CA72-4,CEA and AFP tumor markers combined with multimodal MRI have high sensitivity and specificity in diagnosing rectal cancer stage and differentiation.Their diagnostic efficacy is significantly better than that of single tests,which can effectively improve the predictive ability of rectal cancer stage and differentiation,provide a more reliable diagnostic reference for clinical practice,and have important clinical significance.展开更多
Nanoscale surface topography is an effective approach in modulating cell-material interactions,significantly impacting cellular and nuclear morphologies,as well as their functionality.However,the adaptive changes in c...Nanoscale surface topography is an effective approach in modulating cell-material interactions,significantly impacting cellular and nuclear morphologies,as well as their functionality.However,the adaptive changes in cellular metabolism induced by the mechanical and geometrical microenvironment of the nanotopography remain poorly understood.In this study,we investigated the metabolic activities in cells cultured on engineered nanopillar substrates by using a label-free multimodal optical imaging platform.This multimodal imaging platform,integrating two photon fluorescence(TPF)and stimulated Raman scattering(SRS)microscopy,allowed us to directly visualize and quantify metabolic activities of cells in 3D at the subcellular scale.We discovered that the nanopillar structure significantly reduced the cell spreading area and circularity compared to flat surfaces.Nanopillar-induced mechanical cues significantly modulate cellular metabolic activities with variations in nanopillar geometry further influencing these metabolic processes.Cells cultured on nanopillars exhibited reduced oxidative stress,decreased protein and lipid synthesis,and lower lipid unsaturation in comparison to those on flat substrates.Hierarchical clustering also revealed that pitch differences in the nanopillar had a more significant impact on cell metabolic activity than diameter variations.These insights improve our understanding of how engineered nanotopographies can be used to control cellular metabolism,offering possibilities for designing advanced cell culture platforms which can modulate cell behaviors and mimic natural cellular environment and optimize cell-based applications.By leveraging the unique metabolic effects of nanopillar arrays,one can develop more effective strategies for directing the fate of cells,enhancing the performance of cell-based therapies,and creating regenerative medicine applications.展开更多
Objective To develop a multimodal imaging atlas of a rat brain-computer interface(BCI)that incorporates brain,arterial,bone tissue and a BCI device using mixed reality(MR)for three-dimensional(3D)visualization.Methods...Objective To develop a multimodal imaging atlas of a rat brain-computer interface(BCI)that incorporates brain,arterial,bone tissue and a BCI device using mixed reality(MR)for three-dimensional(3D)visualization.Methods An invasive BCI was implanted in the left visual cortex of 4-week-old Sprague–Dawley rats.Multimodal imaging techniques,including micro-CT and 9.0 T MRI,were used to acquire images of the rat cranial bone structure,vascular distribution,brain tissue functional zones,and BCI device before and after implantation.Using 3D-slicer software,the images were fused through spatial transformations,followed by image segmentation and 3D model reconstruction.The HoloLens platform was employed for MR visualization.Results This study constructed a multimodal imaging atlas for rats that included the skull,brain tissue,arterial tissue,and BCI device coupled with MR technology to create an interactive 3D anatomical model.Conclusions This multimodal 3D atlas provides an objective and stable reference for exploring complex relationships between brain tissue structure and function,enhancing the understanding of the operational principles of BCIs.This is the first multimodal 3D imaging atlas related to a BCI created using Sprague–Dawley rats.展开更多
Autoimmune pancreatitis(AIP)is a special type of chronic pancreatitis with cli-nical symptoms of obstructive jaundice and abdominal discomfort;this condition is caused by autoimmunity and marked by pancreatic fibrosis...Autoimmune pancreatitis(AIP)is a special type of chronic pancreatitis with cli-nical symptoms of obstructive jaundice and abdominal discomfort;this condition is caused by autoimmunity and marked by pancreatic fibrosis and dysfunction.Previous studies have revealed a close relationship between early pancreatic atrophy and the incidence rate of diabetes in type 1 AIP patients receiving steroid treatment.Shimada et al performed a long-term follow-up study and reported that the pancreatic volume(PV)of these patients initially exponentially decreased but then slowly decreased,which was considered to be an important factor related to diabetes;moreover,serum IgG4 levels were positively correlated with PV during follow-up.In this letter,regarding the original study presented by Shimada et al,we present our insights and discuss how multimodal medical imaging and arti-ficial intelligence can be used to better assess the relationship between pancreatic morphological changes and diabetes in patients with AIP.展开更多
Medical image fusion technology is crucial for improving the detection accuracy and treatment efficiency of diseases,but existing fusion methods have problems such as blurred texture details,low contrast,and inability...Medical image fusion technology is crucial for improving the detection accuracy and treatment efficiency of diseases,but existing fusion methods have problems such as blurred texture details,low contrast,and inability to fully extract fused image information.Therefore,a multimodal medical image fusion method based on mask optimization and parallel attention mechanism was proposed to address the aforementioned issues.Firstly,it converted the entire image into a binary mask,and constructed a contour feature map to maximize the contour feature information of the image and a triple path network for image texture detail feature extraction and optimization.Secondly,a contrast enhancement module and a detail preservation module were proposed to enhance the overall brightness and texture details of the image.Afterwards,a parallel attention mechanism was constructed using channel features and spatial feature changes to fuse images and enhance the salient information of the fused images.Finally,a decoupling network composed of residual networks was set up to optimize the information between the fused image and the source image so as to reduce information loss in the fused image.Compared with nine high-level methods proposed in recent years,the seven objective evaluation indicators of our method have improved by 6%−31%,indicating that this method can obtain fusion results with clearer texture details,higher contrast,and smaller pixel differences between the fused image and the source image.It is superior to other comparison algorithms in both subjective and objective indicators.展开更多
Immunotherapy assays using immunoadjuvants and tumor antigens could greatly increase the survival rates of patients with malignant tumors.As effective carriers,metal-organic frameworks(MOFs)have been widely utilized i...Immunotherapy assays using immunoadjuvants and tumor antigens could greatly increase the survival rates of patients with malignant tumors.As effective carriers,metal-organic frameworks(MOFs)have been widely utilized in cancer therapy due to their remarkable histocompatibility and low toxicity.Herein,we constructed a multimodal imaging-guided synergistic cancer photoimmunotherapy by employing a specific MOF(MIL101-NH2)as the core carrier;the MOF was dual-dressed with photoacoustic and fluorescent signal donors(indocyanine green,ICG)and immune adjuvants(cytosine-phosphate-guanine sequence,CpG)and named ICGCpG@MOF.This nanocarrier could passively target the tumor site through the EPR effect and achieve multimodal imaging(fluorescence,photoacoustic,photothermal and magnetic resonance imaging)of the tumor.Synergistic cancer photoimmunotherapy was achieved via simultaneous photodynamic and photothermal methods with 808 nm laser irradiation.ICG-CpG@MOF achieved the GSH-controlled release of immunoadjuvant into the tumor microenvironment.Furthermore,the released tumor-associated antigen along with CpG could induce the transformation of tumor cells from cold to hot by activating the immune system,which significantly enhanced tumor cytotoxicity and achieved high cure rates with minimal side-effects.This strategy utilizing multimodal imaging and synergistic cancer photoimmunotherapy provides a promising approach for the diagnosis and treatment of cancer.展开更多
Accurate diagnosis of tumors needs much detailed information. However, available single imaging modality cannot provide complete or comprehensive data. Nanomedicine is the application of nanotechnology to medicine, an...Accurate diagnosis of tumors needs much detailed information. However, available single imaging modality cannot provide complete or comprehensive data. Nanomedicine is the application of nanotechnology to medicine, and multimodality imaging based on nanoparticles has been receiving extensive attention. This new hybrid imaging technology could provide complementary information from different imaging modalities using only a single injection of contrast agent. In this review, we introduce recent developments in multifunctional nanoparticles and their biomedical applications to multimodal imaging and theragnosis as nanomedicine. Most of the reviewed studies are based on the intrinsic properties of nanoparticles and their application in clinical imaging technology. The imaging techniques include positron emission tomography, single-photon emission computed tomography, computerized tomography, magnetic resonance imaging, optical imaging, and ultrasound imaging.展开更多
基金supported in part by funding from BeiGene,Ltd.,USA(Grant No.:KPR081)with additional support from the Alessandra Bono Foundation,Italy.
文摘Pamiparib is a potent and selective oral poly(adenosine diphosphate(ADP)-ribose)-polymerase(PARP)1/2inhibitor(PARPi).Pamiparib has good bioavailability and shows greater cytotoxic potency and similar DNA-trapping capacity compared to olaparib.It is not affected by adenosine triphosphate(ATP)-binding cassette transporters.
文摘BACKGROUND Congenital renal arteriovenous fistula(RAVF)is a clinically rare condition and frequently missed and misdiagnosed.Multimodal imaging techniques can pro-vide more detailed diagnostic information to help physicians more accurately diagnose and treat diseases.Combining imaging methods to diagnose RAVF has rarely been reported.CASE SUMMARY A 69-year-old female patient presented with gross hematuria that had persisted for 10 days.The patient underwent ultrasound examinations of the kidneys and renal blood vessels,enhanced computed tomography,three-dimensional com-puted tomography angiography,and digital subtraction angiography of the renal arteries.These revealed dilatation of the left renal vein and abnormal shunting between the left renal artery and vein.The patient was diagnosed with a left RAVF using combined multimodal imaging techniques.The patient was treated with left renal artery embolization immediately after renal arteriography.Hema-turia resolved following the left renal artery embolization without serious bleeding or other complications.The patient made a full recovery after one year of postoperative follow-up.CONCLUSION Multimodal imaging techniques complement each other when diagnosing RAVF,providing detailed diagnostic information that can aid in accurate diagnosis and treatment.In addition,this case reminds the sonographer to pay more attention to the color doppler flow imaging and blood flow spectrum when examining the kidney,so as to avoid misdiagnosis of renal cystic lesions as renal cysts and missed diagnosis of RAVF.
文摘AIM:To describe the multimodal imaging features,treatment,and outcomes of patients diagnosed with adultonset Coats disease.METHODS:This retrospective study included patients first diagnosed with Coats disease at≥18 years of age between September 2017 and September 2021.Some patients received anti-vascular endothelial growth factor(VEGF)therapy(conbercept,0.5 mg)as the initial treatment,which was combined with laser photocoagulation as needed.All the patients underwent best corrected visual acuity(BCVA)and intraocular pressure examinations,fundus color photography,spontaneous fluorescence tests,fundus fluorescein angiography,optical coherence tomography(OCT),OCT angiography,and other examinations.BCVA alterations and multimodal image findings in the affected eyes following treatment were compared and the prognostic factors were analyzed.RESULTS:The study included 15 patients who were aged 24-72(57.33±12.61)y at presentation.Systemic hypertension was the most common associated systemic condition,occurring in 13(86.7%)patients.Baseline BCVA ranged from 2.0 to 5.0(4.0±1.1),which showed improvement following treatment(4.2±1.0).Multimodal imaging revealed retinal telangiectasis in 13 patients(86.7%),patchy hemorrhage in 5 patients(33.3%),and stage 2B disease(Shield’s staging criteria)in 11 patients(73.3%).OCT revealed that the baseline central macular thickness(CMT)ranged from 129 to 964μm(473.0±230.1μm),with 13 patients(86.7%)exhibiting a baseline CMT exceeding 250μm.Furthermore,8 patients(53.3%)presented with an epiretinal membrane at baseline or during follow-up.Hyper-reflective scars were observed on OCT in five patients(33.3%)with poor visual prognosis.Vision deteriorated in one patient who did not receive treatment.Final vision was stable in three patients who received laser treatment,whereas improvement was observed in one of two patients who received anti-VEGF therapy alone.In addition,8 of 9 patients(88.9%)who received laser treatment and conbercept exhibited stable or improved BCVA.CONCLUSION:Multimodal imaging can help diagnose adult-onset Coats disease.Anti-VEGF treatment combined with laser therapy can be an option for improving or maintaining BCVA and resolving macular edema.The final visual outcome depends on macular involvement and the disease stage.
基金Supported by the Technology Innovation Leading Program of Shaanxi,No.2023KXJ-095the Shaanxi Provincial People's Hospital Science and Technology Talent Support Program for Elite Talents,No.2021JY-38 and No.2021JY-50the Shaanxi Provincial People's Hospital Science and Technology Development Incubation Foundation,No.2023YJY-39.
文摘BACKGROUND Giant cell tumor of bone is a locally aggressive and rarely metastasizing tumor,and also a potential malignant tumor that may develop into a primary malignant giant cell tumor.AIM To evaluate the role of multimodal imaging in the diagnosis of giant cell tumors of bone.METHODS The data of 32 patients with giant cell tumor of bone confirmed by core-needle biopsy or surgical pathology at our hospital between March 2018 and March 2023 were retrospectively selected.All the patients with giant cell tumors of the bone were examined by X-ray,computed tomography(CT)and magnetic resonance imaging(MRI),and 7 of them were examined by positron emission tomography(PET)-CT.RESULTS X-ray imaging can provide overall information on giant cell tumor lesions.CT and MRI can reveal the characteristics of the internal structure of the tumor as well as the adjacent relationships of the tumor,and these methods have unique advantages for diagnosing tumors and determining the scope of surgery.PET-CT can detect small lesions and is highly valuable for identifying benign and malignant tumors to aid in the early diagnosis of metastasis.CONCLUSION Multimodal imaging plays an important role in the diagnosis of giant cell tumor of bone and can provide a reference for the treatment of giant cell tumors.
文摘BACKGROUND The rare co-occurrence of oligodendroglioma and arteriovenous malformation(AVM)in the same intracranial location.CASE SUMMARY In a 61-year-old man presenting with progressive headaches,is described in this case study.Preoperative multimodal imaging techniques(computed tomography,magnetic resonance imaging,magnetic resonance spectroscopy,digital subtraction angiography,and computed tomography angiography)were employed to detect hemorrhage,cystic and solid lesions,and arteriovenous shunting in the right temporal lobe.The patient underwent right temporal craniotomy for lesion removal,and postoperative pathological analysis confirmed the presence of oligodendroglioma(World Health Organization grade II,not otherwise specified)and AVM.CONCLUSION The preoperative utilization of multimodal imaging examination can help clinicians reduce the likelihood of misdiagnosis or oversight of these conditions,and provides important information for subsequent treatment.This case supports the feasibility of craniotomy for the removal of glioma with AVM.
文摘AIM:To describe the clinical characteristics of eyes using multimodal imaging features with acute macular neuroretinopathy(AMN)lesions following severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection.METHODS:Retrospective case series study.From December 18,2022 to February 14,2023,previously healthy cases within 1-week infection with SARS-CoV-2 and examined at Tianjin Eye Hospital to confirm the diagnosis of AMN were included in the study.Totally 5 males and 9 females[mean age:29.93±10.32(16-49)y]were presented for reduced vision,with or without blurred vision.All patients underwent best corrected visual acuity(BCVA),intraocular pressure,slit lamp microscopy,indirect fundoscopy.Simultaneously,multimodal imagings fundus photography(45°or 200°field of view)was performed in 7 cases(14 eyes).Near infrared(NIR)fundus photography was performed in 9 cases(18 eyes),optical coherence tomography(OCT)in 5 cases(10 eyes),optical coherence tomography angiography(OCTA)in 9 cases(18 eyes),and fundus fluorescence angiography(FFA)in 3 cases(6 eyes).Visual field was performed in 1 case(2 eyes).RESULTS:Multimodal imaging findings data from 14 patients with AMN were reviewed.All eyes demonstrated different extent hyperreflective lesions at the level of the inner nuclear layer and/or outer plexus layer on OCT or OCTA.Fundus photography(45°or 200°field of view)showed irregular hypo-reflective lesion around the fovea in 7 cases(14 eyes).OCTA demonstrated that the superficial retinal capillary plexus(SCP)vascular density,deep capillary plexus(DCP)vascular density and choriocapillaris(CC)vascular density was reduced in 9 case(18 eyes).Among the follow-up cases(2 cases),vascular density increased in 1 case with elevated BCVA;another case has vascular density decrease in one eye and basically unchanged in other eye.En face images of the ellipsoidal zone and interdigitation zone injury showed a low wedge-shaped reflection contour appearance.NIR image mainly show the absence of the outer retinal interdigitation zone in AMN.No abnormal fluorescence was observed in FFA.Corresponding partial defect of the visual field were visualized via perimeter in one case.CONCLUSION:The morbidity of SARS-CoV-2 infection with AMN is increased.Ophthalmologists should be aware of the possible,albeit rare,AMN after SARS-CoV-2 infection and focus on multimodal imaging features.OCT,OCTA,and infrared fundus phase are proved to be valuable tools for detection of AMN in patients with SARS-CoV-2.
文摘Multimodal imaging,including augmented or mixed reality,transforms the physicians’interaction with clinical imaging,allowing more accurate data interpretation,better spatial resolution,and depth perception of the patient’s anatomy.We successfully overlay 3D holographic visualization to magnetic resonance imaging images for preoperative decision making of a complex case of cardiac tumour in a 7-year-old girl.
基金supported by the National Natural Science Foundation of China (Nos.51772293,U1932112,and 21471103)Beijing Natural Science Foundation (No.2202064)+1 种基金Science and Technology Innovation Service Ability Construction Project of the Beijing Municipal Commission of Education (No.19530050182)CAS Key Laboratory of Nano-Bio Interface (No.20NBI01)。
文摘Developing low toxicity and multifunctional theranostic nanoplatform is the key for precise cancer diagnosis and treatment.Herein,an inorganic-organic hybrid nanocomposite is designed by modifying zirconium dioxide(ZrO_(2)) with polydopamine(PDA) followed by doping Mn^(2+) ions and functionalizing with Tween 20(Tween-ZrO_(2)@PDA-Mn2+) for multimodal imaging and chemo-photothermal combination therapy.The as-prepared nanocomposite exhibits good biocompatibility in vitro and in vivo.Specifically,it can be employed as a multifunctional platform not only for computed tomography(CT)imaging and T1-weighted magnetic resonance(MR) imaging,but also for efficient chemotherapeutic drug doxorubicin hydrochloride(DOX) loading.Importantly,because of the pronounced photothermal conversion performance and controllable DOX release ability triggered by the near-infrared(NIR)irradiation and acidic pH,the synergistic effect between photothermal the rapy and chemotherapy results in an enhanced cancer treatment efficacy in vivo.Our work provides a high-performance inorganicorganic hybrid nanotheranostic platform for chemo-photothermal cancer therapy guided by CT and MR imaging.
基金We acknowledge financial support from grants MYRG2014-00093-FHS,MYRG 2015-00036-FHS,MYRG2016-00110-FHS and MYRG2018-00081-FHS from the University of Macao in Macao and grants FDCT 0011/2018/A1 and FDCT 025/2015/A1 from the Macao government.
文摘Photoacoustic imaging(PAI)is a hybrid imaging method based on photoacoustic(PA)effects,which is able to capture the structure,function,and molecular information of biological tissues with high resolution.To date,therapeutic techniques under the guidance of PAI have provided new strategies for accurate diagnosis and precise treatment of tumors.In particular,conjugated polymer nanoparticles have been extensively inspected for PA-based cancer theranostics largely due to their superior optical properties such as tunable spectrum and large absorption coefficient and their good biocompatibility,and abundant functional groups.This mini-review mainly focuses on the recent advances toward the development of novel conjugated polymer nanoparticles for PA-based multimodal imaging and cancer photothermal therapy.
文摘AIM： To describe retinal findings of various imaging modalities in acute retinal ischemia. METHODS： Fluorescein angiography（FA）, spectral domain optical coherence tomography（SD-OCT）, OCTangiography（OCT-A） and fundus autofluorescence（FAF） images of 13 patients（mean age 64y, range 28-86y） with acute retinal ischemia were evaluated. Six suffered from branch arterial occlusion, 2 had a central retinal artery occlusion, 2 had a combined arteriovenous occlusions, 1 patient had a retrobulbar arterial compression by an orbital haemangioma and 2 patients showed an ocular ischemic syndrome.RESULTS： All patients showed increased reflectivity and thickening of the ischemic retinal tissue. In 10 out of 13 patients SD-OCT revealed an additional highly reflective band located within or above the outer plexiform layer. Morphological characteristics were a decreasing intensity with distance from the fovea, partially segmental occurrence and manifestation limited in time. OCT-A showed a loss of flow signal in the superficial and deep capillary plexus at the affected areas. Reduced flow signal was detected underneath the regions with retinal edema. FAF showed areas of altered signal intensity at the posterior pole. The regions of decreased FAF signal corresponded to peri-venous regions. CONCLUSION： Multimodal imaging modalities in retinal ischemia yield characteristic findings and valuable diagnostic information. Conventional OCT identifies hyperreflectivity and thickening and a mid-retinal hyperreflective band is frequently observed. OCT-A examination reveals demarcation of the ischemic retinal area on the vascular level. FAF shows decreased fluorescence signal in areas of retinal edema often corresponding to peri-venous regions.
基金Supported by the Clinical Research Plan of SHDC(No.SHDC2020CR6029).
文摘The phenotypes of the adenine-to-guanine transition at position 3243 of mitochondrial DNA(m.3243A>G)are highly variable,with different symptoms observed in different patients.These include mitochondrial encephalomyopathy,lactic acidosis,and stroke-like episodes(MELAS);maternally inherited diabetes and deafness syndrome(MIDD);other syndromic conditions;or non-syndromic mitochondrial disorders.Renal involvement associated with this mutation generally manifests as subnephrotic proteinuria,progressive deterioration of kidney function,and increased morbidity.The retinopathies linked to the m.3243A>G mutation have heterogeneous presentations,characterized by variable degrees of retinal pigment epithelium(RPE)atrophy and hyperpigmentation at the posterior pole.As a severe phenotype of the m.3243A>G mutation,MELAS combined with focal and segmental glomerulosclerosis(FSGS)is rare.We herein firstly reported in detail the ophthalmic manifestations of a patient with this condition.Additionally,we reviewed the literature on fundus,ophthalmic electrophysiology,and optical coherence tomography(OCT)findings related to the m.3243A>G mutation.
基金the National Natural Science Foundation of China(62375127,82272664)Hunan Provincial Natural Science Foundation of China(2022JJ30843)+5 种基金the Science and Technology Development Fund Guided by Central Govern-ment(2021Szvup169)the Scientic Research Program of Hunan Provincial Health Commission(B202304077077)the Fundamental Research Funds for the Central Universities(NS2022035)Prospective Layout Special Fund of Nanjing University of Aero-nautics and Astronautics(ILA-22022)Graduate Research and Innovation Program of Nanjing University of Aeronautics and Astronautics(xcxjh20220328)Experimental Technology Research and Development Project of NUAA(No.SYJS202303Z)for the grant。
文摘Osteosarcoma is the most common primary bone tumor with high malignancy.It is particularly necessary to achieve rapid and accurate diagnosis in its intraoperative examination and early diagnosis.Accordingly,the multimodal microscopic imaging diagnosis system constructed by bright field,spontaneous fluorescence and polarized light microscopic imaging was used to study the pathological mechanism of osteosarcoma from the tissue microenvironment level and achieve rapid and accurate diagnosis.First,the multimodal microscopic images of normal and osteosarcoma tissue slices were collected to characterize the overall morphology of the tissue microenvironment of the samples,the arrangement structure of collagen fibers and the content and distribution of endogenous fluorescent substances.Second,based on the correlation and complementarity of the feature information contained in the three single-mode images,combined with convolutional neural network(CNN)and image fusion methods,a multimodal intelligent diagnosis model was constructed to effectively improve the information utilization and diagnosis accuracy.The accuracy and true positivity of the multimodal diagnostic model were significantly improved to 0.8495 and 0.9412,respectively,compared to those of the single-modal models.Besides,the difference of tissue microenvironments before and after cancerization can be used as a basis for cancer diagnosis,and the information extraction and intelligent diagnosis of osteosarcoma tissue can be achieved by using multimodal microscopic imaging technology combined with deep learning,which significantly promoted the application of tissue microenvironment in pathological examination.This diagnostic system relies on its advantages of simple operation,high efficiency and accuracy and high cost-effectiveness,and has enormous clinical application potential and research significance.
基金support from Shenzhen Medical Research Fund(B2402042)National Natural Science Foundation of China(22274069 and 22304070)+1 种基金Shenzhen Science and Technology Program project(JCYJ20240813094504007 and JCYJ20210324104007020)Guangdong Provincial Key Laboratory of Advanced Biomaterials(2022B1212010003).
文摘Near-infrared Ⅱ(NIR-Ⅱ)fluorescent nanoparticles(NPs)based on aggregation-induced emission(AIE)have attracted significant attention due to theirmultimodal imaging capabilities aswell as the combined photothermal and photodynamic therapeutic effects in cancer therapy.Reported herein is the rational designed AIE molecule(BPT),via incorporating phenothiazine units with strong electron-donating and reactive oxygen species(ROS)generation capabilities into the classical AIE scaffold tetraphenylethylene,further coupled with a strong electron-acceptor named benzo[1,2-c:4,5-c’]bis[1,2,5]thiadiazole.The BPT NPs exhibited maximum NIR-Ⅱ fluorescence emission at 1083 nm,a fluorescence quantum yield of 1.53%,photothermal conversion efficiency of 63%,and photoacoustic imaging capabilities,alongside considerable type I ROS generation ability.Additionally,when a kind of nitric oxide(NO)donor named O_(2)-(2,4-dinitrophenyl)1-[(4-ethoxycarbonyl)piperazin-1-yl]diazen-1-ium-1,2-diolate(JSK)was incorporated,the corresponding JSK-BPT NPs could generate O_(2)^(−),NO,and peroxynitrite to induce phototoxicity.By applying it to the 4T1 breast tumor model,JSK-BPT NPs achieved high-quality multimodal imaging of the vasculature and tumor regions in mice.Under the multimodal imaging guidance,the 4T1 tumor could be ablated completely after a single dose of JSK-BPT NPs and under the irradiation of an 808 nm laser.
基金Supported by the Zhejiang Provincial Natural Science Foundation of China,No.LTGY24H160006Jiaxing Medical Key Discipline,No.2023-ZC-015.
文摘BACKGROUND Rectal cancer is one of the common digestive system malignant tumors around the world.Its early diagnosis and staging are crucial for rectal cancer treatment and prognosis.In recent years,tumor markers have gradually received attention in early screening,treatment monitoring and prognostic evaluation of cancer,but their predictive role in rectal cancer staging and differentiation is still unclear.AIM To assess the prognostic value of tumor markers alpha-fetoprotein(AFP)cancer antigen 72-4(CA72-4),carbohydrate antigen 19-9(CA19-9),and carcinoembryonic antigen(CEA),alongside multimodal magnetic resonance imaging(MRI),for staging and differentiating rectal cancer in patients.METHODS This study retrospectively analyzed 167 patients with rectal cancer who were treated at our institution from January 2020 to December 2024.Each patient underwent serological testing and multimodal MRI for diagnosis.Histopathological examination after surgical resection or imaging based on follow-up was used as the gold standard.According to the T stage and differentiation degree,patients were divided into low stage group(T1-T2)and high stage group(T3-T4).In addition,they were divided into low-differentiation groups and high-differentiation groups according to their differentiation degree.We compared the accuracy,sensitivity and specificity of tumor marker levels and MRI in rectal cancer stage and differentiation.RESULTS The study's findings indicate that in the context of rectal cancer T staging,there is substantial concordance between MRI and clinicopathological assessments,with a Kappa coefficient of 0.789(P<0.001).Similarly,for various degrees of tumor differentiation,MRI and clinicopathological evaluations demonstrated substantial agreement,with a Kappa coefficient of 0.651(P<0.001).Notably,the concentrations of tumor markers CA19-9,CA72-4,CEA,and AFP were significantly elevated in the T3-T4 stage compared to the T1-T2 stage.Furthermore,these markers were significantly higher in the low-differentiation group compared to the high-differentiation group(P<0.05).The combined use of tumor markers and MRI for preoperative T staging of rectal cancer yielded a diagnostic sensitivity of 93.7%and a specificity of 94.6%,as evidenced by the receiver operating characteristic analysis,with an area under the curve of 0.947.For tumor differentiation,the diagnostic sensitivity and specificity were 93.6%and 97.1%,respectively,with an area under the curve of 0.978(95%confidence interval:0.946-1.000),surpassing the accuracy of individual detection methods.CONCLUSION The CA19-9,CA72-4,CEA and AFP tumor markers combined with multimodal MRI have high sensitivity and specificity in diagnosing rectal cancer stage and differentiation.Their diagnostic efficacy is significantly better than that of single tests,which can effectively improve the predictive ability of rectal cancer stage and differentiation,provide a more reliable diagnostic reference for clinical practice,and have important clinical significance.
基金supported by the National Science Foundation(Grant ECCS-2025752)supported by Air Force Office of Scientific Research YIP award(AFOSR FA9550-23-1-0090)and Cancer research coordinating committee faculty seed grant to Z.J.We acknowledge support from NIH R01GM149976,NIH U01AI167892,NIH 5R01NS111039,NIH R21NS125395,U54CA132378,Sloan Research Fellow Award,and Hellman Fellow Award.
文摘Nanoscale surface topography is an effective approach in modulating cell-material interactions,significantly impacting cellular and nuclear morphologies,as well as their functionality.However,the adaptive changes in cellular metabolism induced by the mechanical and geometrical microenvironment of the nanotopography remain poorly understood.In this study,we investigated the metabolic activities in cells cultured on engineered nanopillar substrates by using a label-free multimodal optical imaging platform.This multimodal imaging platform,integrating two photon fluorescence(TPF)and stimulated Raman scattering(SRS)microscopy,allowed us to directly visualize and quantify metabolic activities of cells in 3D at the subcellular scale.We discovered that the nanopillar structure significantly reduced the cell spreading area and circularity compared to flat surfaces.Nanopillar-induced mechanical cues significantly modulate cellular metabolic activities with variations in nanopillar geometry further influencing these metabolic processes.Cells cultured on nanopillars exhibited reduced oxidative stress,decreased protein and lipid synthesis,and lower lipid unsaturation in comparison to those on flat substrates.Hierarchical clustering also revealed that pitch differences in the nanopillar had a more significant impact on cell metabolic activity than diameter variations.These insights improve our understanding of how engineered nanotopographies can be used to control cellular metabolism,offering possibilities for designing advanced cell culture platforms which can modulate cell behaviors and mimic natural cellular environment and optimize cell-based applications.By leveraging the unique metabolic effects of nanopillar arrays,one can develop more effective strategies for directing the fate of cells,enhancing the performance of cell-based therapies,and creating regenerative medicine applications.
基金supported by the National Natural Science Foundation of China(No.82172524 and No.81974355)National Innovation Platform Development Program(No.2020021105012440),China+3 种基金Major Program of Hubei Province(No.2021BEA161),ChinaMajor Key Project of Hubei Province(No.JD2023BAA005),ChinaWuhan Union Hospital Free Innovation Preliminary Research Fund(No.2024XHYN047),ChinaJoint Funds for the Innovation of Science and Technology(No.2024Y9062),Fujian Province,China.
文摘Objective To develop a multimodal imaging atlas of a rat brain-computer interface(BCI)that incorporates brain,arterial,bone tissue and a BCI device using mixed reality(MR)for three-dimensional(3D)visualization.Methods An invasive BCI was implanted in the left visual cortex of 4-week-old Sprague–Dawley rats.Multimodal imaging techniques,including micro-CT and 9.0 T MRI,were used to acquire images of the rat cranial bone structure,vascular distribution,brain tissue functional zones,and BCI device before and after implantation.Using 3D-slicer software,the images were fused through spatial transformations,followed by image segmentation and 3D model reconstruction.The HoloLens platform was employed for MR visualization.Results This study constructed a multimodal imaging atlas for rats that included the skull,brain tissue,arterial tissue,and BCI device coupled with MR technology to create an interactive 3D anatomical model.Conclusions This multimodal 3D atlas provides an objective and stable reference for exploring complex relationships between brain tissue structure and function,enhancing the understanding of the operational principles of BCIs.This is the first multimodal 3D imaging atlas related to a BCI created using Sprague–Dawley rats.
文摘Autoimmune pancreatitis(AIP)is a special type of chronic pancreatitis with cli-nical symptoms of obstructive jaundice and abdominal discomfort;this condition is caused by autoimmunity and marked by pancreatic fibrosis and dysfunction.Previous studies have revealed a close relationship between early pancreatic atrophy and the incidence rate of diabetes in type 1 AIP patients receiving steroid treatment.Shimada et al performed a long-term follow-up study and reported that the pancreatic volume(PV)of these patients initially exponentially decreased but then slowly decreased,which was considered to be an important factor related to diabetes;moreover,serum IgG4 levels were positively correlated with PV during follow-up.In this letter,regarding the original study presented by Shimada et al,we present our insights and discuss how multimodal medical imaging and arti-ficial intelligence can be used to better assess the relationship between pancreatic morphological changes and diabetes in patients with AIP.
基金supported by Gansu Natural Science Foundation Programme(No.24JRRA231)National Natural Science Foundation of China(No.62061023)Gansu Provincial Education,Science and Technology Innovation and Industry(No.2021CYZC-04)。
文摘Medical image fusion technology is crucial for improving the detection accuracy and treatment efficiency of diseases,but existing fusion methods have problems such as blurred texture details,low contrast,and inability to fully extract fused image information.Therefore,a multimodal medical image fusion method based on mask optimization and parallel attention mechanism was proposed to address the aforementioned issues.Firstly,it converted the entire image into a binary mask,and constructed a contour feature map to maximize the contour feature information of the image and a triple path network for image texture detail feature extraction and optimization.Secondly,a contrast enhancement module and a detail preservation module were proposed to enhance the overall brightness and texture details of the image.Afterwards,a parallel attention mechanism was constructed using channel features and spatial feature changes to fuse images and enhance the salient information of the fused images.Finally,a decoupling network composed of residual networks was set up to optimize the information between the fused image and the source image so as to reduce information loss in the fused image.Compared with nine high-level methods proposed in recent years,the seven objective evaluation indicators of our method have improved by 6%−31%,indicating that this method can obtain fusion results with clearer texture details,higher contrast,and smaller pixel differences between the fused image and the source image.It is superior to other comparison algorithms in both subjective and objective indicators.
基金This work was supported by grants from the National Natural Science Foundation of China,China(21904145,81972019 and 31700150)China Postdoctoral Science Foundation,China(2018M633273)+1 种基金Chen Jingyu team of Sanming Project of Medicine in Shenzhen,China(SZSM201812058)Guangdong Provincial Science and Technology Plan project,China(No.2017B030314108).
文摘Immunotherapy assays using immunoadjuvants and tumor antigens could greatly increase the survival rates of patients with malignant tumors.As effective carriers,metal-organic frameworks(MOFs)have been widely utilized in cancer therapy due to their remarkable histocompatibility and low toxicity.Herein,we constructed a multimodal imaging-guided synergistic cancer photoimmunotherapy by employing a specific MOF(MIL101-NH2)as the core carrier;the MOF was dual-dressed with photoacoustic and fluorescent signal donors(indocyanine green,ICG)and immune adjuvants(cytosine-phosphate-guanine sequence,CpG)and named ICGCpG@MOF.This nanocarrier could passively target the tumor site through the EPR effect and achieve multimodal imaging(fluorescence,photoacoustic,photothermal and magnetic resonance imaging)of the tumor.Synergistic cancer photoimmunotherapy was achieved via simultaneous photodynamic and photothermal methods with 808 nm laser irradiation.ICG-CpG@MOF achieved the GSH-controlled release of immunoadjuvant into the tumor microenvironment.Furthermore,the released tumor-associated antigen along with CpG could induce the transformation of tumor cells from cold to hot by activating the immune system,which significantly enhanced tumor cytotoxicity and achieved high cure rates with minimal side-effects.This strategy utilizing multimodal imaging and synergistic cancer photoimmunotherapy provides a promising approach for the diagnosis and treatment of cancer.
文摘Accurate diagnosis of tumors needs much detailed information. However, available single imaging modality cannot provide complete or comprehensive data. Nanomedicine is the application of nanotechnology to medicine, and multimodality imaging based on nanoparticles has been receiving extensive attention. This new hybrid imaging technology could provide complementary information from different imaging modalities using only a single injection of contrast agent. In this review, we introduce recent developments in multifunctional nanoparticles and their biomedical applications to multimodal imaging and theragnosis as nanomedicine. Most of the reviewed studies are based on the intrinsic properties of nanoparticles and their application in clinical imaging technology. The imaging techniques include positron emission tomography, single-photon emission computed tomography, computerized tomography, magnetic resonance imaging, optical imaging, and ultrasound imaging.
