Autologous and allogeneic bone grafts remain the gold standard for repairing bone defects.However,donor shortages and postoperative infections contribute to unsatisfactory treatment outcomes.Tissue engineering technol...Autologous and allogeneic bone grafts remain the gold standard for repairing bone defects.However,donor shortages and postoperative infections contribute to unsatisfactory treatment outcomes.Tissue engineering technology that utilizes biologically active composites to accelerate the healing and reconstruction of segmental bone defects has led to new ideas for in situ bone repair.Multifunctional nanocomposite hydrogels were constructed by covalently binding silver(Ag^(+))core-embedded mesoporous silica nanoparticles(Ag@MSN)to bone morphogenetic protein-2(BMP-2),which was encapsulated into silk fibroin methacryloyl(SilMA)and photo-crosslinked to form an Ag@MSN-BMP-2/SilMA hydrogel to preserve the biological activity of BMP-2 and slow its release.More importantly,multifunctional Ag^(+)-containing nanocomposite hydrogels showed antibacterial properties.These hydrogels possessed synergistic osteogenic and antibacterial effects to promote bone defect repair.Ag@MSN-BMP-2/SilMA exhibited good biocompatibility in vitro and in vivo owing to its interconnected porosity and improved hydrophilicity.Furthermore,the multifunctional nanocomposite hydrogel showed controllable sustained-release activity that promoted bone regeneration in repairing rat skull defects by inducing osteogenic differentiation and neovascularization.Overall,Ag@MSN-BMP-2/SilMA hydrogels enrich bone regeneration strategies and show great potential for bone regeneration.展开更多
Photothermal therapy(PTT)has presented its inherent application value in cancer treatment.Nevertheless,single-functional photothermal materials cannot meet the precise diagnosis and treatment of cance r.Therefo re,it ...Photothermal therapy(PTT)has presented its inherent application value in cancer treatment.Nevertheless,single-functional photothermal materials cannot meet the precise diagnosis and treatment of cance r.Therefo re,it is important to design a nanocomposite that has both high therapeutic efficiency and multimodal imaging capabilities.In our work,a new nanostructure of gold nanorods(AuNRs)with silver shells decorated by BaGdF5:Yb^(3+),Er^(3+)nanoparticle was synthesized by a simple way.The biostability of AuNRs is increased by coating with silver shells,and the AuNR@Ag nanoparticles can be used as excellent surface enhanced Raman scattering(SERS)probe.Moreover,the modification of BaGdF5:Yb^(3+),Er^(3+)nanoparticles provides the possibility of real-time optical imaging of the tumor area.Under the irradiation of NIR laser,the AuNR@Ag/BaGdF5:Yb^(3+),Er^(3+)nanocomposites(NCs)have strong upconversion emission and excellent photothermal conversion efficiency.Meanwhile,the NCs show low cytotoxicity and good biocompatibility in MTT cytotoxicity test.Moreover,the NCs are also exceptional contrast agents for CT imaging.For in vitro photothermal therapy test,NCs show excellent killing efficiency on tumor cells.Therefore,the multifaceted research of AuNR@Ag/BaGdF5:Yb^(3+),Er^(3+)multifunctional nanomaterials provides a break for high-efficiency tumor photothermal therapy and multimodal imaging.展开更多
基金supported by grants from the 512 Talents Development Project of Bengbu Medical College(Grant Nos by51202302 and by51202309)the Domestic Visiting and Training Program for Outstanding Young Backbone Teachers in High Schools(Grant No.gxgnfx2022036)+2 种基金the Natural Science Research Project of the Anhui Educational Committee(Grant Nos KJ2021ZD0089 and 2022AH020086)the Scientific Research Foundation of Bengbu Medical College(Grant No.2021bypd006)the Distinguished Young Scholars of First Affiliated Hospital of Bengbu Medical College(Grant No.2021byyfyjq01).
文摘Autologous and allogeneic bone grafts remain the gold standard for repairing bone defects.However,donor shortages and postoperative infections contribute to unsatisfactory treatment outcomes.Tissue engineering technology that utilizes biologically active composites to accelerate the healing and reconstruction of segmental bone defects has led to new ideas for in situ bone repair.Multifunctional nanocomposite hydrogels were constructed by covalently binding silver(Ag^(+))core-embedded mesoporous silica nanoparticles(Ag@MSN)to bone morphogenetic protein-2(BMP-2),which was encapsulated into silk fibroin methacryloyl(SilMA)and photo-crosslinked to form an Ag@MSN-BMP-2/SilMA hydrogel to preserve the biological activity of BMP-2 and slow its release.More importantly,multifunctional Ag^(+)-containing nanocomposite hydrogels showed antibacterial properties.These hydrogels possessed synergistic osteogenic and antibacterial effects to promote bone defect repair.Ag@MSN-BMP-2/SilMA exhibited good biocompatibility in vitro and in vivo owing to its interconnected porosity and improved hydrophilicity.Furthermore,the multifunctional nanocomposite hydrogel showed controllable sustained-release activity that promoted bone regeneration in repairing rat skull defects by inducing osteogenic differentiation and neovascularization.Overall,Ag@MSN-BMP-2/SilMA hydrogels enrich bone regeneration strategies and show great potential for bone regeneration.
基金Project supported by National key R&D Program of China(2019YFA0705204)National Natural Science Foundation of China(51072026,51573023,51802027)。
文摘Photothermal therapy(PTT)has presented its inherent application value in cancer treatment.Nevertheless,single-functional photothermal materials cannot meet the precise diagnosis and treatment of cance r.Therefo re,it is important to design a nanocomposite that has both high therapeutic efficiency and multimodal imaging capabilities.In our work,a new nanostructure of gold nanorods(AuNRs)with silver shells decorated by BaGdF5:Yb^(3+),Er^(3+)nanoparticle was synthesized by a simple way.The biostability of AuNRs is increased by coating with silver shells,and the AuNR@Ag nanoparticles can be used as excellent surface enhanced Raman scattering(SERS)probe.Moreover,the modification of BaGdF5:Yb^(3+),Er^(3+)nanoparticles provides the possibility of real-time optical imaging of the tumor area.Under the irradiation of NIR laser,the AuNR@Ag/BaGdF5:Yb^(3+),Er^(3+)nanocomposites(NCs)have strong upconversion emission and excellent photothermal conversion efficiency.Meanwhile,the NCs show low cytotoxicity and good biocompatibility in MTT cytotoxicity test.Moreover,the NCs are also exceptional contrast agents for CT imaging.For in vitro photothermal therapy test,NCs show excellent killing efficiency on tumor cells.Therefore,the multifaceted research of AuNR@Ag/BaGdF5:Yb^(3+),Er^(3+)multifunctional nanomaterials provides a break for high-efficiency tumor photothermal therapy and multimodal imaging.
