Co-assembly of ABC linear triblock copolymer/nanoparticle into bump-surface multicompartment hybrids in selective solvent was studied through self-consistent field theory （SCFT） simulation. Results from three-dimens...Co-assembly of ABC linear triblock copolymer/nanoparticle into bump-surface multicompartment hybrids in selective solvent was studied through self-consistent field theory （SCFT） simulation. Results from three-dimensional SCFT simulation showed that the hybrid morphology depended on the length and number of grafted chains, whereas the number and shape of bumps relied on nanoparticle size. Moreover, the simulation results showed that the length and number of grafted chains had equivalent effect on hybrid morphology. Calculated results indicated that entropy was a more important factor than enthalpy in the co-assembly.展开更多
In this paper, pod-like supramicelles with multicompartment hydrophobic cores were prepared by selfassembly of amphiphilic N-phthaloylchitosan-g-poly(N-vinylcaprolactam)(PHCS-g-PNVCL) in aqueous medium. The employed b...In this paper, pod-like supramicelles with multicompartment hydrophobic cores were prepared by selfassembly of amphiphilic N-phthaloylchitosan-g-poly(N-vinylcaprolactam)(PHCS-g-PNVCL) in aqueous medium. The employed biocompatible amphiphilic polymer was synthesized by grafting the carboxyl terminated poly(N-vinylcaprolactam)(PNVCL-COOH) chains onto N-phthaloylchitosan(PHCS) backbone.~1H NMR and FTIR results confirmed the molecular structure of the copolymers. The morphology of the supramicelles assembled by PHCS-g-PNVCL was revealed by means of TEM and polarized light microscope. In solution, the supramicelles were very stable as monitored by DLS and zeta potential measurements. Temperature and p H presented significant influences on the size and size distribution of the supramicelles. These supramicelles with multicompartment hydrophobic cores should be ideal biomimetic systems with promising applications in drug delivery.展开更多
Inspired by the intricate eukaryotic cell structure,the spontaneously assembling multicompartments capable of spatiotemporally regulated biomacromolecules transportation is still a challenge.Here,a heterogeneous prote...Inspired by the intricate eukaryotic cell structure,the spontaneously assembling multicompartments capable of spatiotemporally regulated biomacromolecules transportation is still a challenge.Here,a heterogeneous proteinosome-based multicompartment was designed and constructed by combining aqueous two-phase system and Pickering emulsion methods.By loading PEGylated insulin with boronic acid group into the multicompartment and glucose oxidase(GOx)into the innermost microgel domain,a short-or long-term transportation pathway of the loaded insulin was constructed by responding to the concentration of glucose.Moreover,the diminution of dynamic boronic ester bonding,the hindrance of polyethylene oxide micro-chamber,electrostatic interaction and the swelling behavior of hydrogel triggered by GOx/glucose reaction resulted in spatiotemporally controlled multi-pathway transportation mode with the releasing of insulin loaded inside the innermost microgel at high concentration of glucose(10 mg·mL^(-1)).Specially,by incorporating rhodamine B and fluorescein labelled BSA into the multicompartment,the release procedure of the loaded insulin can be monitored by the fluorescence color-change.Overall,a multimode microcompartment is constructed which is then expected to provide a promising platform for further therapeutic protein,gene and drug delivery,as well as towards the design of complicated cell biomimetics.展开更多
Dissipative particle dynamics simulations were performed on the morphology and structure of multicompartmerit micelles formed from n-shaped ABC block copolymers in water. The influences of chain architectures were stu...Dissipative particle dynamics simulations were performed on the morphology and structure of multicompartmerit micelles formed from n-shaped ABC block copolymers in water. The influences of chain architectures were studied in a systematic way, and a rich variety of morphologies were observed, such as spherical, wormlike, X-shaped, Y-shaped, ribbon-like, layered rod-like, layered disk-like, as well as network morphologies. The simulations show that the distance between the two grafts plays an important role in control of the morphology. Since n-shaped ABC block copolymers can be reduced to linear ABC and star ABC block copolymers, they are good model copolymers for studying the self-assembly of complex block copolymers into micelles. The knowledge obtained in this work as well as the new morphologies identified provide useful information for future rational design and synthesis of novel multicompartment micelles.展开更多
The rapid development of microfluidic technology has led to the evolution of microdroplets from simple emulsion structures to complex multilayered and multicompartmental configurations.These advancements have endowed ...The rapid development of microfluidic technology has led to the evolution of microdroplets from simple emulsion structures to complex multilayered and multicompartmental configurations.These advancements have endowed microdroplets with the capability to contain multiple compartments that remain isolated from one another,enabling them to carry different molecules of interest.Consequently,researchers can now investigate intricate spatially confined chemical reactions and signal transduction pathways within subcellular organelles.Moreover,modern microdroplets often possess excellent optical transparency,allowing fluorescently labelled,multi-layered,and compartmental droplets to provide detailed insights through real-time,in situ,and dynamic fluorescence imaging.Hence,this review systematically summarizes current methodologies for preparing multicomponent microdroplets and their applications,particularly focusing on fluorescent microdroplets.Additionally,it discusses existing critical challenges and outlines future research directions.By offering a comprehensive overview of the preparation methods and applications of fluorescent microdroplets,this review aims to stimulate the interest of researchers and foster their utilization in more complex and biomimetic environments.展开更多
基金supported by the National Natural Science Foundation of China for General Program(No. 21274145)
文摘Co-assembly of ABC linear triblock copolymer/nanoparticle into bump-surface multicompartment hybrids in selective solvent was studied through self-consistent field theory （SCFT） simulation. Results from three-dimensional SCFT simulation showed that the hybrid morphology depended on the length and number of grafted chains, whereas the number and shape of bumps relied on nanoparticle size. Moreover, the simulation results showed that the length and number of grafted chains had equivalent effect on hybrid morphology. Calculated results indicated that entropy was a more important factor than enthalpy in the co-assembly.
基金NSFC Grants(5140306251273063 and 20774030)+4 种基金China Postdoctoral Science Foundation(2013M541485)111 Project Grant(B08021)the Fundamental Research Funds for the Central Universitiesthe higher school specialized research fund for the doctoral program(20110074110003)the Open Project of Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Bingtuan(2015BTRC001)for support of this work
文摘In this paper, pod-like supramicelles with multicompartment hydrophobic cores were prepared by selfassembly of amphiphilic N-phthaloylchitosan-g-poly(N-vinylcaprolactam)(PHCS-g-PNVCL) in aqueous medium. The employed biocompatible amphiphilic polymer was synthesized by grafting the carboxyl terminated poly(N-vinylcaprolactam)(PNVCL-COOH) chains onto N-phthaloylchitosan(PHCS) backbone.~1H NMR and FTIR results confirmed the molecular structure of the copolymers. The morphology of the supramicelles assembled by PHCS-g-PNVCL was revealed by means of TEM and polarized light microscope. In solution, the supramicelles were very stable as monitored by DLS and zeta potential measurements. Temperature and p H presented significant influences on the size and size distribution of the supramicelles. These supramicelles with multicompartment hydrophobic cores should be ideal biomimetic systems with promising applications in drug delivery.
基金We thank NSFC(Nos.51873050 and 21871069)for financial support.
文摘Inspired by the intricate eukaryotic cell structure,the spontaneously assembling multicompartments capable of spatiotemporally regulated biomacromolecules transportation is still a challenge.Here,a heterogeneous proteinosome-based multicompartment was designed and constructed by combining aqueous two-phase system and Pickering emulsion methods.By loading PEGylated insulin with boronic acid group into the multicompartment and glucose oxidase(GOx)into the innermost microgel domain,a short-or long-term transportation pathway of the loaded insulin was constructed by responding to the concentration of glucose.Moreover,the diminution of dynamic boronic ester bonding,the hindrance of polyethylene oxide micro-chamber,electrostatic interaction and the swelling behavior of hydrogel triggered by GOx/glucose reaction resulted in spatiotemporally controlled multi-pathway transportation mode with the releasing of insulin loaded inside the innermost microgel at high concentration of glucose(10 mg·mL^(-1)).Specially,by incorporating rhodamine B and fluorescein labelled BSA into the multicompartment,the release procedure of the loaded insulin can be monitored by the fluorescence color-change.Overall,a multimode microcompartment is constructed which is then expected to provide a promising platform for further therapeutic protein,gene and drug delivery,as well as towards the design of complicated cell biomimetics.
基金Project supported by the National Natural Science Foundation of China （No. 20676004）.
文摘Dissipative particle dynamics simulations were performed on the morphology and structure of multicompartmerit micelles formed from n-shaped ABC block copolymers in water. The influences of chain architectures were studied in a systematic way, and a rich variety of morphologies were observed, such as spherical, wormlike, X-shaped, Y-shaped, ribbon-like, layered rod-like, layered disk-like, as well as network morphologies. The simulations show that the distance between the two grafts plays an important role in control of the morphology. Since n-shaped ABC block copolymers can be reduced to linear ABC and star ABC block copolymers, they are good model copolymers for studying the self-assembly of complex block copolymers into micelles. The knowledge obtained in this work as well as the new morphologies identified provide useful information for future rational design and synthesis of novel multicompartment micelles.
基金the National Nature Science Foundation of China(No.22107028)Natural Science Foundation of Chongqing(No.CSTB2023NSCQ-MSX0335)。
文摘The rapid development of microfluidic technology has led to the evolution of microdroplets from simple emulsion structures to complex multilayered and multicompartmental configurations.These advancements have endowed microdroplets with the capability to contain multiple compartments that remain isolated from one another,enabling them to carry different molecules of interest.Consequently,researchers can now investigate intricate spatially confined chemical reactions and signal transduction pathways within subcellular organelles.Moreover,modern microdroplets often possess excellent optical transparency,allowing fluorescently labelled,multi-layered,and compartmental droplets to provide detailed insights through real-time,in situ,and dynamic fluorescence imaging.Hence,this review systematically summarizes current methodologies for preparing multicomponent microdroplets and their applications,particularly focusing on fluorescent microdroplets.Additionally,it discusses existing critical challenges and outlines future research directions.By offering a comprehensive overview of the preparation methods and applications of fluorescent microdroplets,this review aims to stimulate the interest of researchers and foster their utilization in more complex and biomimetic environments.
