The study was conducted to investigate the effects of replacing antibiotics using multi-enzyme preparations on growth performance,coefficient of total tract apparent digestibility,digestive enzyme activity,and antioxi...The study was conducted to investigate the effects of replacing antibiotics using multi-enzyme preparations on growth performance,coefficient of total tract apparent digestibility,digestive enzyme activity,and antioxidant property in piglets.A total of 160 piglets（（21.35±0.22）kg）were randomly assigned to five dietary treatments：1）basal diet supplemented with antibiotics（AC）,2）antibiotic diet supplemented with 0.5 g kg^-1 multi-enzyme preparations（AC＋0.5EP）,3）antibiotic diet supplemented with 1.5 g kg^-1 multi-enzyme preparations（AC＋1.5EP）,4）basal diet supplemented with a half dosage of antibiotics and 1.5 g kg^-1 multi-enzyme preparations（AH＋1.5EP）,and 5）basal diet supplemented with 1.5 g kg^-1 multienzyme preparations（BC＋1.5EP）.The results showed that AC＋1.5EP significantly improved the feed efficiency,apparent digestibility of ether extract（EE）and crude ash（CA）,lipase activity in pancreas and duodenum content,maltase and lactase activity in jejunum and ileum mucosa,glutathione peroxidase（GSH-Px）concentration in serum and liver,and decreased malondialdehyde（MDA）concentration in serum and liver compared with piglets receiving AC（P〈0.05）.Piglets receiving BC＋1.5EP showed no significant difference in growth performance（P〉0.05）but had lower MDA concentration than piglets receiving AC（P〈0.05）.The apparent digestibility of EE and crude fiber（CF）,duodenal lipase activity,jejunum mucosa maltase,and ileum mucosa lactase activity of piglets receiving AH＋1.5EP or BC＋1.5EP were significantly improved compared with piglets receiving AC（P〈0.05）.These results indicated an additive growth promotion effect between antibiotics and multi-enzyme preparations on piglets,and the multi-enzyme preparations may be used as substitutes for antibiotics for improving piglet production performance and health status.展开更多
This experiment was conducted to determine whether the performance of broilers fed diets based on corn and soybean meal could be enhanced with enzymes or probiotics. A total of 120 male broilers, three days of age, we...This experiment was conducted to determine whether the performance of broilers fed diets based on corn and soybean meal could be enhanced with enzymes or probiotics. A total of 120 male broilers, three days of age, were assigned to one of four treatments in a completely randomized design, and housed in groups of five with six cages per treatment. The control diet was based on corn and soybean meal while the three experimental diets consisted of the basal diet supplemented with 0.1% of enzyme I, enzyme II, or probiotic. Enzyme I provided α-galactosidase and fl-mannanase, while enzyme II provided protease, amylase, α-galactosidase, xylanase, and cellulase. The probiotic was composed of Bacillus coagulance, Bacillus lichenformis , Bacillus subtilis , and Clostridium butyricum. Over the 28 day experiment, the weight gain of birds fed the probiotic treatment was superior （P = 0.03 ） to the control, while gains for the enzyme treatments were intermediate to those of the control and probiotic. Feed intake and feed conversion did not differ among treatments （P 〉 0.05 ）. Ammonia production was significantly （ P 〈 0.01 ） higher in the control compared with either of the enzyme or probiotic treatments. Compared with the control, supplementation with enzyme H significantly reduced the digestibility of arginine, isoleucine, and lysine （P 〈 0.05 ）. In contrast, the digestibility of energy was higher （P 〈 0.01 ） for birds supplemented with enzyme II than the control. Digestibility coeffi- cients did not differ for any other parameter with the exception of energy which was significantly higher for birds fed the probiotic treatment than the control （P 〈 0.01 ）. In summary, the performance of broilers was significantly enhanced by the addition of a probiotic to the diet. However, under the conditions of this experi- ment, supplementation with a multi-enzyme complex containing either α-galactosidase and fl-mannanase or the combination of protease, amylase, galactosidase, xylanase, and cellulase failed to improve broiler performance.展开更多
Phosphorylated sugars,recognized as central intermediates in carbohydrate metabolism and critical precursors for enzymatic synthesis of rare sugars,face significant technical barriers in their industrialscale producti...Phosphorylated sugars,recognized as central intermediates in carbohydrate metabolism and critical precursors for enzymatic synthesis of rare sugars,face significant technical barriers in their industrialscale production.The multi-enzymatic preparation systems for these compounds inherently accumulate complex impurities,including protein-based catalysts,residual substrates,and oligosaccharide byproducts,posing persistent challenges in product separation and biocatalyst recycling.To address this limitation,we conducted a systematic investigation of ultrafiltration-based separation strategies during the multi-enzyme-catalyzed synthesis of fructose-1,6-bisphosphate(FDP),with particular emphasis on membrane fouling mechanisms.By screening the ultrafiltration membranes,UE020 showed the best performance in the model system,achieving significant separation targets:99.97% retention of bovine serum albumin,FDP/maltodextrin separation coefficient of 7.41,and FDP recovery of 93.63%.An analysis of the components of resistance revealed that concentration polarization induced by maltodextrin was the main factor constituting the resistance,irreversible resistance due to bovine serum albumin was a secondary effect,and the resistance constituted by FDP was negligible.A mitigation strategy employing powdered activated carbon for dynamic membrane formation significantly improved system performance,reducing irreversible resistance by 59.14% and enhancing flux recovery by 20.85%.In this study,ultrafiltration was strategically employed to achieve efficient separation of FDP and enzyme recovery.Significantly,we deciphered the synergistic fouling mechanisms arising from interactions within the multicomponent system containing phosphorylated sugars,oligosaccharides,and proteins.These findings provide a mechanistic framework for scaling up multi-enzymatic systems dedicated to phosphorylated sugar biosynthesis,effectively bridging the gap between laboratory-scale synthesis and industrial implementation.展开更多
基金financially supported by the National 863 Program of China (2013AA102803D)
文摘The study was conducted to investigate the effects of replacing antibiotics using multi-enzyme preparations on growth performance,coefficient of total tract apparent digestibility,digestive enzyme activity,and antioxidant property in piglets.A total of 160 piglets（（21.35±0.22）kg）were randomly assigned to five dietary treatments：1）basal diet supplemented with antibiotics（AC）,2）antibiotic diet supplemented with 0.5 g kg^-1 multi-enzyme preparations（AC＋0.5EP）,3）antibiotic diet supplemented with 1.5 g kg^-1 multi-enzyme preparations（AC＋1.5EP）,4）basal diet supplemented with a half dosage of antibiotics and 1.5 g kg^-1 multi-enzyme preparations（AH＋1.5EP）,and 5）basal diet supplemented with 1.5 g kg^-1 multienzyme preparations（BC＋1.5EP）.The results showed that AC＋1.5EP significantly improved the feed efficiency,apparent digestibility of ether extract（EE）and crude ash（CA）,lipase activity in pancreas and duodenum content,maltase and lactase activity in jejunum and ileum mucosa,glutathione peroxidase（GSH-Px）concentration in serum and liver,and decreased malondialdehyde（MDA）concentration in serum and liver compared with piglets receiving AC（P〈0.05）.Piglets receiving BC＋1.5EP showed no significant difference in growth performance（P〉0.05）but had lower MDA concentration than piglets receiving AC（P〈0.05）.The apparent digestibility of EE and crude fiber（CF）,duodenal lipase activity,jejunum mucosa maltase,and ileum mucosa lactase activity of piglets receiving AH＋1.5EP or BC＋1.5EP were significantly improved compared with piglets receiving AC（P〈0.05）.These results indicated an additive growth promotion effect between antibiotics and multi-enzyme preparations on piglets,and the multi-enzyme preparations may be used as substitutes for antibiotics for improving piglet production performance and health status.
文摘This experiment was conducted to determine whether the performance of broilers fed diets based on corn and soybean meal could be enhanced with enzymes or probiotics. A total of 120 male broilers, three days of age, were assigned to one of four treatments in a completely randomized design, and housed in groups of five with six cages per treatment. The control diet was based on corn and soybean meal while the three experimental diets consisted of the basal diet supplemented with 0.1% of enzyme I, enzyme II, or probiotic. Enzyme I provided α-galactosidase and fl-mannanase, while enzyme II provided protease, amylase, α-galactosidase, xylanase, and cellulase. The probiotic was composed of Bacillus coagulance, Bacillus lichenformis , Bacillus subtilis , and Clostridium butyricum. Over the 28 day experiment, the weight gain of birds fed the probiotic treatment was superior （P = 0.03 ） to the control, while gains for the enzyme treatments were intermediate to those of the control and probiotic. Feed intake and feed conversion did not differ among treatments （P 〉 0.05 ）. Ammonia production was significantly （ P 〈 0.01 ） higher in the control compared with either of the enzyme or probiotic treatments. Compared with the control, supplementation with enzyme H significantly reduced the digestibility of arginine, isoleucine, and lysine （P 〈 0.05 ）. In contrast, the digestibility of energy was higher （P 〈 0.01 ） for birds supplemented with enzyme II than the control. Digestibility coeffi- cients did not differ for any other parameter with the exception of energy which was significantly higher for birds fed the probiotic treatment than the control （P 〈 0.01 ）. In summary, the performance of broilers was significantly enhanced by the addition of a probiotic to the diet. However, under the conditions of this experi- ment, supplementation with a multi-enzyme complex containing either α-galactosidase and fl-mannanase or the combination of protease, amylase, galactosidase, xylanase, and cellulase failed to improve broiler performance.
基金the funding support provided by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDC0120402)the National Key Research&Development Program of China (2022YFC2105103)。
文摘Phosphorylated sugars,recognized as central intermediates in carbohydrate metabolism and critical precursors for enzymatic synthesis of rare sugars,face significant technical barriers in their industrialscale production.The multi-enzymatic preparation systems for these compounds inherently accumulate complex impurities,including protein-based catalysts,residual substrates,and oligosaccharide byproducts,posing persistent challenges in product separation and biocatalyst recycling.To address this limitation,we conducted a systematic investigation of ultrafiltration-based separation strategies during the multi-enzyme-catalyzed synthesis of fructose-1,6-bisphosphate(FDP),with particular emphasis on membrane fouling mechanisms.By screening the ultrafiltration membranes,UE020 showed the best performance in the model system,achieving significant separation targets:99.97% retention of bovine serum albumin,FDP/maltodextrin separation coefficient of 7.41,and FDP recovery of 93.63%.An analysis of the components of resistance revealed that concentration polarization induced by maltodextrin was the main factor constituting the resistance,irreversible resistance due to bovine serum albumin was a secondary effect,and the resistance constituted by FDP was negligible.A mitigation strategy employing powdered activated carbon for dynamic membrane formation significantly improved system performance,reducing irreversible resistance by 59.14% and enhancing flux recovery by 20.85%.In this study,ultrafiltration was strategically employed to achieve efficient separation of FDP and enzyme recovery.Significantly,we deciphered the synergistic fouling mechanisms arising from interactions within the multicomponent system containing phosphorylated sugars,oligosaccharides,and proteins.These findings provide a mechanistic framework for scaling up multi-enzymatic systems dedicated to phosphorylated sugar biosynthesis,effectively bridging the gap between laboratory-scale synthesis and industrial implementation.
