High-density linkage maps are essential tools for genome analysis of various biological traits. Our developed compact multi-gel system, HEGS （high efficiency genome scanning） is a high-throughput and high-cost-perfo...High-density linkage maps are essential tools for genome analysis of various biological traits. Our developed compact multi-gel system, HEGS （high efficiency genome scanning） is a high-throughput and high-cost-performance electrophoresis apparatus. Using this system, a high-density （average interval 2.3 cM） map with 1 065 AFLP and 63 SSR markers was constructed from recombinant inbred lines of a japonica and indica hybrid in just two months of electrophoreses by a single person. More than 50% of the mapped AFLP markers were commonly polymorphic for several combinations between japonica and indica rice and 15% were applicable for genetically closer crosses between upland and lowland types of japonica rice. This system can be used for rapid analyses of all kinds of markers.展开更多
This paper presents a fuzzy control algorithm applied to the position control of a multi-axis motion platform to achieve high precision motion control of the multi-axis motion platform.A LabVIEW-based multi-axis motio...This paper presents a fuzzy control algorithm applied to the position control of a multi-axis motion platform to achieve high precision motion control of the multi-axis motion platform.A LabVIEW-based multi-axis motion control system is designed.This system controls stepper motors using trapezoidal acceleration/deceleration pulse types and fuzzy control algorithms,which effectively avoids mechanical jitter and loss of step in the process of multi-angle motion of the stepper motor,and achieves accurate control of the stepper motor.The TCP/IP(transmission control protocol/internet protocol)communication protocol is used,so that data are output stably and not lost in the process of transmission and communication,achieving the purpose of interconnection of different systems and remote control of equipment.This control system has been tested to maintain a high level of stability and repeatability during actual operation.展开更多
Bamboos are one of the most beautiful and useful plants on Earth.The genetic background and population structure of bamboos are well known,which helps accelerate the process of artificial domestication of bamboo.Parti...Bamboos are one of the most beautiful and useful plants on Earth.The genetic background and population structure of bamboos are well known,which helps accelerate the process of artificial domestication of bamboo.Partial sequences of six genes involved in nitrogen use efficiency in 32 different bamboo species were analyzed for occurrence of single nucleotide polymorphisms(SNPs).The nucleotide diversityθw and total nucleotide polymorphismsπT of the sequenced DNA regions was 0.05137 and 0.03332,respectively.Bothπnonsyn/πsyn and Ka/Ks values were<1.The nucleotide sequences of these six genes were inferred to be relatively conserved,and the haplotype diversity was relatively high.The results of evolutionary neutrality tests showed that the six genes were in line with neutral evolution,and that the NRT2.1 and AMT2.1 gene sequences may have experienced negative selection.An inter-SNP recombination event at the NRT2.1 gene in the all pooled sample,of all 32 bamboo species was the lowest at 0.0645,whereas the AMT gene recombination events were all>0.1.Estimation and analysis of linkage disequilibrium of five genes revealed that with the increase in nucleotide sequence length,the degree of SNP linkage disequilibrium decreased rapidly.We inferred the population genetic structure of 32 bamboo species based on the SNP loci of six genes with frequencies>18%.32 bamboo species were divided into five categories,which indicated that the combined population of all bamboo species had obvious multivariate characteristics and was heterogeneous;red(Group 1)and green(Group 2)were the main groups.展开更多
Based on the panel data of 29 provincial administrative regions in China from 2011 to 2022,this study uses the entropy weighting method and the global super-efficiency SBM model to evaluate and measure the public libr...Based on the panel data of 29 provincial administrative regions in China from 2011 to 2022,this study uses the entropy weighting method and the global super-efficiency SBM model to evaluate and measure the public libraries construction level and innovation efficiency in China,uses the vector autoregression model,theαconvergence model and theβconvergence model to evaluate and test whether there is a positive linkage effect between public libraries construction and innovation efficiency,and uses the coupling coordination model to calculate and analyze the coupling coordination degree between public libraries construction and innovation efficiency.The results show that China’s public libraries construction and innovation efficiency are generally in a good development trend,but there is still a large gap from reaching a high level.There is a positive linkage effect between China’s public libraries construction and innovation efficiency,which can not only promote each other’s development,but also drive each other to achieve regional balanced development.The coupling coordination degree between China’s public libraries construction and innovation efficiency is showing a steady upward trend,but it is still on the verge of imbalance as a whole,and the eastern region is significantly better than the central region and western region.The results reveal the positive linkage effect between public libraries construction and innovation efficiency,which has enlightenment significance for building a strong country in culture and innovation.Based on this,this study puts forward suggestions on increasing investment in public libraries construction,optimizing the configuration structure of innovation factors,and strengthening the deep integration of public libraries construction and innovation activities,so as to give full play to the positive linkage effect between public libraries construction and innovation efficiency.展开更多
While laser surface texturing(LST)is a promising manufacturing technique for surface functionalization,simultaneously realizing high precision and high efficiency in the LST of complex curved surface is challenging,du...While laser surface texturing(LST)is a promising manufacturing technique for surface functionalization,simultaneously realizing high precision and high efficiency in the LST of complex curved surface is challenging,due to continuously varied geometries of laser-matter incidence.In the present work,we propose a novel manufacturing system of 7-axis on-the-fly LST for complex curved surface,based on the integrated synchronization of 5-axis linkage motion platform with 2-axis galvanometer.Specifically,the algorithm for decomposing spatial texture trajectory on curved surface into low-frequency and high-frequency parts is established,based on which the kinematic model of synchronized 7-axis system is developed to derive the motion of each axis in both 5-axis linkage motion platform and 2-axis galvanometer simultaneously.Subsequently,the synchronized 7-axis LST system is experimentally realized,including the setup of mechanical stages integrated with optical path,the configuration of numerical control unit,and the development of processing software.Finally,case study of 7-axis on-the-fly LST of freeform aluminum surface is performed,and the advantages in terms of processing efficiency and texturing accuracy over 5-axis linkage LST are demonstrated.The correlation of reduced following errors between mechanical stages with the promoted performance of curved surface texturing by the 7-axis on-the-fly LST is further analyzed.Current work provides a feasible solution for establishing the manufacturing system for high performance LST of complex curved surface.展开更多
文摘High-density linkage maps are essential tools for genome analysis of various biological traits. Our developed compact multi-gel system, HEGS （high efficiency genome scanning） is a high-throughput and high-cost-performance electrophoresis apparatus. Using this system, a high-density （average interval 2.3 cM） map with 1 065 AFLP and 63 SSR markers was constructed from recombinant inbred lines of a japonica and indica hybrid in just two months of electrophoreses by a single person. More than 50% of the mapped AFLP markers were commonly polymorphic for several combinations between japonica and indica rice and 15% were applicable for genetically closer crosses between upland and lowland types of japonica rice. This system can be used for rapid analyses of all kinds of markers.
文摘This paper presents a fuzzy control algorithm applied to the position control of a multi-axis motion platform to achieve high precision motion control of the multi-axis motion platform.A LabVIEW-based multi-axis motion control system is designed.This system controls stepper motors using trapezoidal acceleration/deceleration pulse types and fuzzy control algorithms,which effectively avoids mechanical jitter and loss of step in the process of multi-angle motion of the stepper motor,and achieves accurate control of the stepper motor.The TCP/IP(transmission control protocol/internet protocol)communication protocol is used,so that data are output stably and not lost in the process of transmission and communication,achieving the purpose of interconnection of different systems and remote control of equipment.This control system has been tested to maintain a high level of stability and repeatability during actual operation.
基金This study was financially supported by the National Natural Science Foundation of China(41301346)the Natural Science Foundation of Fujian Province(2020J01375)the Natural Science Foundation of Fujian Province(2015N0034).
文摘Bamboos are one of the most beautiful and useful plants on Earth.The genetic background and population structure of bamboos are well known,which helps accelerate the process of artificial domestication of bamboo.Partial sequences of six genes involved in nitrogen use efficiency in 32 different bamboo species were analyzed for occurrence of single nucleotide polymorphisms(SNPs).The nucleotide diversityθw and total nucleotide polymorphismsπT of the sequenced DNA regions was 0.05137 and 0.03332,respectively.Bothπnonsyn/πsyn and Ka/Ks values were<1.The nucleotide sequences of these six genes were inferred to be relatively conserved,and the haplotype diversity was relatively high.The results of evolutionary neutrality tests showed that the six genes were in line with neutral evolution,and that the NRT2.1 and AMT2.1 gene sequences may have experienced negative selection.An inter-SNP recombination event at the NRT2.1 gene in the all pooled sample,of all 32 bamboo species was the lowest at 0.0645,whereas the AMT gene recombination events were all>0.1.Estimation and analysis of linkage disequilibrium of five genes revealed that with the increase in nucleotide sequence length,the degree of SNP linkage disequilibrium decreased rapidly.We inferred the population genetic structure of 32 bamboo species based on the SNP loci of six genes with frequencies>18%.32 bamboo species were divided into five categories,which indicated that the combined population of all bamboo species had obvious multivariate characteristics and was heterogeneous;red(Group 1)and green(Group 2)were the main groups.
基金Supported by the Zhejiang Province Soft Science Research Project(2023C35006)。
文摘Based on the panel data of 29 provincial administrative regions in China from 2011 to 2022,this study uses the entropy weighting method and the global super-efficiency SBM model to evaluate and measure the public libraries construction level and innovation efficiency in China,uses the vector autoregression model,theαconvergence model and theβconvergence model to evaluate and test whether there is a positive linkage effect between public libraries construction and innovation efficiency,and uses the coupling coordination model to calculate and analyze the coupling coordination degree between public libraries construction and innovation efficiency.The results show that China’s public libraries construction and innovation efficiency are generally in a good development trend,but there is still a large gap from reaching a high level.There is a positive linkage effect between China’s public libraries construction and innovation efficiency,which can not only promote each other’s development,but also drive each other to achieve regional balanced development.The coupling coordination degree between China’s public libraries construction and innovation efficiency is showing a steady upward trend,but it is still on the verge of imbalance as a whole,and the eastern region is significantly better than the central region and western region.The results reveal the positive linkage effect between public libraries construction and innovation efficiency,which has enlightenment significance for building a strong country in culture and innovation.Based on this,this study puts forward suggestions on increasing investment in public libraries construction,optimizing the configuration structure of innovation factors,and strengthening the deep integration of public libraries construction and innovation activities,so as to give full play to the positive linkage effect between public libraries construction and innovation efficiency.
基金the support by the Harbin Manufacturing Science and Technology Innovation Talent Project(No.2023CXRCGD035)the Open Research Foundation of State Key Laboratory of Digital Manufacturing Equipment and Technology in Huazhong University of Science and Technology,China(No.IMETKF2023012).
文摘While laser surface texturing(LST)is a promising manufacturing technique for surface functionalization,simultaneously realizing high precision and high efficiency in the LST of complex curved surface is challenging,due to continuously varied geometries of laser-matter incidence.In the present work,we propose a novel manufacturing system of 7-axis on-the-fly LST for complex curved surface,based on the integrated synchronization of 5-axis linkage motion platform with 2-axis galvanometer.Specifically,the algorithm for decomposing spatial texture trajectory on curved surface into low-frequency and high-frequency parts is established,based on which the kinematic model of synchronized 7-axis system is developed to derive the motion of each axis in both 5-axis linkage motion platform and 2-axis galvanometer simultaneously.Subsequently,the synchronized 7-axis LST system is experimentally realized,including the setup of mechanical stages integrated with optical path,the configuration of numerical control unit,and the development of processing software.Finally,case study of 7-axis on-the-fly LST of freeform aluminum surface is performed,and the advantages in terms of processing efficiency and texturing accuracy over 5-axis linkage LST are demonstrated.The correlation of reduced following errors between mechanical stages with the promoted performance of curved surface texturing by the 7-axis on-the-fly LST is further analyzed.Current work provides a feasible solution for establishing the manufacturing system for high performance LST of complex curved surface.
