Allergic bronchopulmonary aspergillosis(ABPA) is a complex hypersensitivity syndrome triggered against antigens of Aspergillus fumigatus,a fungus that most commonly colonizes the airways of patients with bronchial ast...Allergic bronchopulmonary aspergillosis(ABPA) is a complex hypersensitivity syndrome triggered against antigens of Aspergillus fumigatus,a fungus that most commonly colonizes the airways of patients with bronchial asthma and cystic fibrosis.It presents clinically with refractory asthma,hemoptysis and systemic manifestations including fever,malaise and weight loss.Radiologically,it presents with central bronchiectasis and recurrent episodes of mucus plugging.The mucus plugs in ABPA are generally hypodense but in up to 20% of patients the mucus can be hyperdense on computed tomography.This paper reviews the literature on the clinical significance of hyperattenuated mucus in patients with ABPA.展开更多
Mucoid degeneration of cruciate ligament is rare cause of knee pain, however there are some cases causing pain and restriction of extension. A 60-year-old man came to our clinic complaining of pain in knee joint on fu...Mucoid degeneration of cruciate ligament is rare cause of knee pain, however there are some cases causing pain and restriction of extension. A 60-year-old man came to our clinic complaining of pain in knee joint on full range of extension about 3 months ago. The range of motion (ROM) was a flexion contracture of 5 degree and a further flexion of 140 degree with pain aggravation by forced extension. Magnetic resonance imaging of the knee joint showed thickened posterior cruciate ligament (PCL) mucoid degeneration. Arthroscopic treatment consists of PCL reduction-plasty by debridement of yellowish material in the PCL fiber and reduces the volume of the hypertrophied PCL. Immediately after surgery, patient gain the full ROM without any symptom of impingement. Enlarged PCL can make impingement in femoral notch with anterior cruciate ligament (ACL). Then, Partial debulking surgery of PCL is an effective treatment to pain relief and restore ROM of knee.展开更多
Hypervirulent Klebsiella pneumoniae(hvKp)is a major cause of severe community-acquired infection.A key plasmidencoded factor,regulator of mucoid phenotype A(RmpA),activates capsule locus gene expression to promote hyp...Hypervirulent Klebsiella pneumoniae(hvKp)is a major cause of severe community-acquired infection.A key plasmidencoded factor,regulator of mucoid phenotype A(RmpA),activates capsule locus gene expression to promote hypermucoviscosity,a phenotype that is strongly linked to increased pathogenicity.However,the precise regulatory mechanisms that control RmpA in hvKp remain poorly understood.In this study,we constructed a rmpA knockdown strain in hvKp using CRISPR interference and assessed the global regulatory role of RmpA through complementary multi-omic sequencing(RNA sequencing[RNA-seq]and chromatin immunoprecipitation sequencing[ChIP-seq]),promoter-gfp reporter assays,and phenotypic experiments.The functional role of RmpA was evaluated in Escherichia coli via heterologous expression.The RNA-seq analysis revealed that RmpA activates carbohydrate metabolism pathways,but represses pathways related to DNA replication,ribosome metabolism,and biofilm formation.The ChIP-seq analysis further confirmed the potential role of RmpA as a global regulator that enhances capsule production by activating transcripts within the capsule locus.It also upregulates genes involved in sugar metabolism and transport,which supplies essential precursors for capsule synthesis.RmpA modulates the phenotypic switch between hypermucoviscosity and biofilm formation by repressing type Ⅲ fimbriae genes.Notably,RmpA overexpression in E.coli induced changes in multiple metabolic pathways.These findings position RmpA as a latent central regulator in hvKp that orchestrates the metabolic pathways and phenotypic traits essential for virulence.展开更多
文摘Allergic bronchopulmonary aspergillosis(ABPA) is a complex hypersensitivity syndrome triggered against antigens of Aspergillus fumigatus,a fungus that most commonly colonizes the airways of patients with bronchial asthma and cystic fibrosis.It presents clinically with refractory asthma,hemoptysis and systemic manifestations including fever,malaise and weight loss.Radiologically,it presents with central bronchiectasis and recurrent episodes of mucus plugging.The mucus plugs in ABPA are generally hypodense but in up to 20% of patients the mucus can be hyperdense on computed tomography.This paper reviews the literature on the clinical significance of hyperattenuated mucus in patients with ABPA.
文摘Mucoid degeneration of cruciate ligament is rare cause of knee pain, however there are some cases causing pain and restriction of extension. A 60-year-old man came to our clinic complaining of pain in knee joint on full range of extension about 3 months ago. The range of motion (ROM) was a flexion contracture of 5 degree and a further flexion of 140 degree with pain aggravation by forced extension. Magnetic resonance imaging of the knee joint showed thickened posterior cruciate ligament (PCL) mucoid degeneration. Arthroscopic treatment consists of PCL reduction-plasty by debridement of yellowish material in the PCL fiber and reduces the volume of the hypertrophied PCL. Immediately after surgery, patient gain the full ROM without any symptom of impingement. Enlarged PCL can make impingement in femoral notch with anterior cruciate ligament (ACL). Then, Partial debulking surgery of PCL is an effective treatment to pain relief and restore ROM of knee.
基金supported by the National Key Research and Development Program of China(grant number 2022YFC2303100 to J.F.)the International Partnership Program of the Chinese Academy of Sciences(grant number 079GJHZ2022033GC to J.F.).
文摘Hypervirulent Klebsiella pneumoniae(hvKp)is a major cause of severe community-acquired infection.A key plasmidencoded factor,regulator of mucoid phenotype A(RmpA),activates capsule locus gene expression to promote hypermucoviscosity,a phenotype that is strongly linked to increased pathogenicity.However,the precise regulatory mechanisms that control RmpA in hvKp remain poorly understood.In this study,we constructed a rmpA knockdown strain in hvKp using CRISPR interference and assessed the global regulatory role of RmpA through complementary multi-omic sequencing(RNA sequencing[RNA-seq]and chromatin immunoprecipitation sequencing[ChIP-seq]),promoter-gfp reporter assays,and phenotypic experiments.The functional role of RmpA was evaluated in Escherichia coli via heterologous expression.The RNA-seq analysis revealed that RmpA activates carbohydrate metabolism pathways,but represses pathways related to DNA replication,ribosome metabolism,and biofilm formation.The ChIP-seq analysis further confirmed the potential role of RmpA as a global regulator that enhances capsule production by activating transcripts within the capsule locus.It also upregulates genes involved in sugar metabolism and transport,which supplies essential precursors for capsule synthesis.RmpA modulates the phenotypic switch between hypermucoviscosity and biofilm formation by repressing type Ⅲ fimbriae genes.Notably,RmpA overexpression in E.coli induced changes in multiple metabolic pathways.These findings position RmpA as a latent central regulator in hvKp that orchestrates the metabolic pathways and phenotypic traits essential for virulence.
