目的 探讨肺结核(tuberculosis, TB)患者血清中涎液化糖链抗原(krebs von den lungen-6, KL-6)、T细胞免疫球蛋白及粘蛋白域蛋白-4(T-cell immunoglobulin and mucin domain-containing molecule-4, TIM-4)水平与病情程度及不良预后的...目的 探讨肺结核(tuberculosis, TB)患者血清中涎液化糖链抗原(krebs von den lungen-6, KL-6)、T细胞免疫球蛋白及粘蛋白域蛋白-4(T-cell immunoglobulin and mucin domain-containing molecule-4, TIM-4)水平与病情程度及不良预后的关系。方法 选取2021年7月至2023年7月期间在江苏省人民医院溧阳分院诊治的158例TB患者为观察组,并选取同期在我院进行健康体检的158例健康者作为对照组。采用Bandim结核病评分标准对TB患者病情程度进行评估,将其分为重症组(n=61)和轻症组(n=97);根据患者预后情况,分为良好组(n=101)与不良组(n=57);采用Logistic回归分析TB患者预后影响因素;通过Spearman相关分析评估血清KL-6和TIM-4水平与TB病情程度的相关性;利用受试者工作特征曲线评价血清KL-6和TIM-4对TB患者预后的预测效能。结果 观察组血清KL-6和TIM-4水平均显著高于对照组(均P<0.05);与轻症组相比,重症组血清KL-6和TIM-4水平均明显升高(均P<0.05);不良组血清KL-6和TIM-4水平均明显高于预后良好组(均P<0.05);血清KL-6、TIM-4水平均是TB患者不良预后的独立影响因素(均P<0.05);血清KL-6、TIM-4预测TB患者预后的曲线下面积(area under curve, AUC)分别为0.789、0.803,2者联合预测的AUC为0.914,2者联合预测效能优于任一单一指标(z2者联合-KL-6=3.375,P=0.001;z2者联合-TIM-4=3.514,P=0.000)。结论 TB患者血清KL-6、TIM-4水平升高,与患者病情严重程度及不良预后密切相关,且2者联合预测对TB患者不良预后具有较高的预测价值。展开更多
Pathogenic Escherichia coli cause chicken colibacillosis, which is economically devastating to the poultry in- dustry worldwide （Bagheri et al., 2014）. Owing to in- creasing antibiotic resistance, phage therapy reag...Pathogenic Escherichia coli cause chicken colibacillosis, which is economically devastating to the poultry in- dustry worldwide （Bagheri et al., 2014）. Owing to in- creasing antibiotic resistance, phage therapy reagents have been developed to treat bacterial infections （Xu et al., 2015）.展开更多
The increasing emergence of multi-drug resistant Escherichia coli(E.coli)has become a global concern,primarily due to the limitation of antimicrobial treatment options.Phage therapy has been considered as a promising ...The increasing emergence of multi-drug resistant Escherichia coli(E.coli)has become a global concern,primarily due to the limitation of antimicrobial treatment options.Phage therapy has been considered as a promising alternative for treating infections caused by multi-drug resistant E.coli.However,the application of phages as a promising antimicrobial agent is limited by their narrow host range and specificity.In this research,a recombinant T4-like phage,named WGqlae,has been obtained by changing the receptor specificity determinant region of gene 37,using a homologous recombination platform of T4-like phages established by our laboratory previously.The engineered phage WGqlae can lyse four additional hosts,comparing to its parental phages WG01 and QL01.WGqlae showed similar characteristics,including thermo and pH stability,optimal multiplicity of infection and one-step growth curve,to the donor phage QL01.In addition,sequencing results showed that gene 37 of recombinant phage WGqlae had genetically stable even after 20 generations.In planktonic test,phage WGqlae had significant antimicrobial effects on E.coli DE192 and DE205 B.The optical density at 600 nm(OD600)of E.coli in phage WGqlae treating group was significantly lower than that of the control group(P\0.01).Besides,phage WGqlae demonstrated an obvious inhibitory effect on the biofilm formation and the clearance of mature biofilms.Our study suggested that engineered phages may be promising candidates for future phage therapy applications against pathogenic E.coli in planktonic and biofilm forms.展开更多
1 Introduction Viruses are the most abundant biological entities on Earth.They can influence the succession of individual microbial populations,biogeochemical cycles of C/N and,ultimately,microbial community structure...1 Introduction Viruses are the most abundant biological entities on Earth.They can influence the succession of individual microbial populations,biogeochemical cycles of C/N and,ultimately,microbial community structure through killing展开更多
文摘目的 探讨肺结核(tuberculosis, TB)患者血清中涎液化糖链抗原(krebs von den lungen-6, KL-6)、T细胞免疫球蛋白及粘蛋白域蛋白-4(T-cell immunoglobulin and mucin domain-containing molecule-4, TIM-4)水平与病情程度及不良预后的关系。方法 选取2021年7月至2023年7月期间在江苏省人民医院溧阳分院诊治的158例TB患者为观察组,并选取同期在我院进行健康体检的158例健康者作为对照组。采用Bandim结核病评分标准对TB患者病情程度进行评估,将其分为重症组(n=61)和轻症组(n=97);根据患者预后情况,分为良好组(n=101)与不良组(n=57);采用Logistic回归分析TB患者预后影响因素;通过Spearman相关分析评估血清KL-6和TIM-4水平与TB病情程度的相关性;利用受试者工作特征曲线评价血清KL-6和TIM-4对TB患者预后的预测效能。结果 观察组血清KL-6和TIM-4水平均显著高于对照组(均P<0.05);与轻症组相比,重症组血清KL-6和TIM-4水平均明显升高(均P<0.05);不良组血清KL-6和TIM-4水平均明显高于预后良好组(均P<0.05);血清KL-6、TIM-4水平均是TB患者不良预后的独立影响因素(均P<0.05);血清KL-6、TIM-4预测TB患者预后的曲线下面积(area under curve, AUC)分别为0.789、0.803,2者联合预测的AUC为0.914,2者联合预测效能优于任一单一指标(z2者联合-KL-6=3.375,P=0.001;z2者联合-TIM-4=3.514,P=0.000)。结论 TB患者血清KL-6、TIM-4水平升高,与患者病情严重程度及不良预后密切相关,且2者联合预测对TB患者不良预后具有较高的预测价值。
基金supported by grants from the Nature Science Foundation of Shandong Province of China (grant nos.ZR2013CQ024 and ZR2015CM020)
文摘Pathogenic Escherichia coli cause chicken colibacillosis, which is economically devastating to the poultry in- dustry worldwide （Bagheri et al., 2014）. Owing to in- creasing antibiotic resistance, phage therapy reagents have been developed to treat bacterial infections （Xu et al., 2015）.
基金supported by Grants from the National Natural Science Foundation of China(U1803109)Key research and development plan of Jiangsu province(BE2019304)+2 种基金National Key R&D Program of China(2018YFC1602500)the Central University Basic Scientific Research Fund-Animal pathogenic bacteria(KYZ201846)Jiangsu modern agriculture(waterfowl)industrial technology system disease prevention and control innovation team(JATS[2018]222)
文摘The increasing emergence of multi-drug resistant Escherichia coli(E.coli)has become a global concern,primarily due to the limitation of antimicrobial treatment options.Phage therapy has been considered as a promising alternative for treating infections caused by multi-drug resistant E.coli.However,the application of phages as a promising antimicrobial agent is limited by their narrow host range and specificity.In this research,a recombinant T4-like phage,named WGqlae,has been obtained by changing the receptor specificity determinant region of gene 37,using a homologous recombination platform of T4-like phages established by our laboratory previously.The engineered phage WGqlae can lyse four additional hosts,comparing to its parental phages WG01 and QL01.WGqlae showed similar characteristics,including thermo and pH stability,optimal multiplicity of infection and one-step growth curve,to the donor phage QL01.In addition,sequencing results showed that gene 37 of recombinant phage WGqlae had genetically stable even after 20 generations.In planktonic test,phage WGqlae had significant antimicrobial effects on E.coli DE192 and DE205 B.The optical density at 600 nm(OD600)of E.coli in phage WGqlae treating group was significantly lower than that of the control group(P\0.01).Besides,phage WGqlae demonstrated an obvious inhibitory effect on the biofilm formation and the clearance of mature biofilms.Our study suggested that engineered phages may be promising candidates for future phage therapy applications against pathogenic E.coli in planktonic and biofilm forms.
基金supported by the National Natural Science Foundation of China (Grant Nos. 41002123 & 41030211)the National Basic Research Program of China (Grant No. 2011CB808800)+1 种基金State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences (No. GBL11201)the Fundamental Research Funds for National University, China University of Geosciences (Wuhan)
文摘1 Introduction Viruses are the most abundant biological entities on Earth.They can influence the succession of individual microbial populations,biogeochemical cycles of C/N and,ultimately,microbial community structure through killing
