The present work focuses on the proximate physical and chemical profile of <em>Opuntia ficus-indica</em> mucilage, mechanically extracted from cladodes, a waste of pruning in traditional organic cactus pea...The present work focuses on the proximate physical and chemical profile of <em>Opuntia ficus-indica</em> mucilage, mechanically extracted from cladodes, a waste of pruning in traditional organic cactus pear orchards in Italy (San Cono, Sicily). The mechanical extraction increased the mucilage yield to 30% dry weight. Physical characterization concerns pH, viscosity, free acidity and density, useful for emulsifying capacity. Spectrophotometric analysis was applied to assess total carbohydrates, proteins, uronic acids, total polyphenols content and antioxidant capacity. DART-MS and SEM-EDX were performed to evaluate functional mucilage components and relative amounts of minerals, respectively. From the main results <em>Opuntia ficus-indica</em> by-product, in addition to the preponderant total carbohydrates content, shows the high concentration in calcium and potassium and a fair amount of health-promoting phytochemicals, which make it a good candidate for the different type of industrial applications.展开更多
The mucilage fraction from Ora-pro-Nobis(OPN)leaves was extracted and characterized regarding its chemical composition,rheological behavior,and prebiotic potential.The extraction involved hot water treatment followed ...The mucilage fraction from Ora-pro-Nobis(OPN)leaves was extracted and characterized regarding its chemical composition,rheological behavior,and prebiotic potential.The extraction involved hot water treatment followed by ethanol precipitation,resulting in a substantial extraction yield(4.77%of total leaves).This mucilage extraction served as an initial step for subsequent protein extraction from residual leaf material.OPN mucilage primarily comprises a high molecular weight polysaccharide(arabinogalactan),which upon hydrolysis,yields xylose(30.11%w/w)and arabinose(29.15%w/w)as primary components,alongside minor amounts of glucose(5.28%w/w)and cellobiose(3.80%w/w).Rheological analysis indicated pseudoplastic behavior and thixotropic properties,offering potential value in formulating 3D printed food products.Furthermore,di-gestibility analysis demonstrated that the mucilage was highly resistant to simulated gastrointestinal conditions,with only 2.69%degradation observed.In addition,assays evaluating its prebiotic potential showed that the mucilage supported the growth of the probiotic strain Lactobacillus acidophilus.Furthermore.All these findings,combined with the classification of glucoarabinoxylan as a dietary or functional fiber,underscore the potential of OPN mucilage as an innovative ingredient for food product development,promoting microbiota balance.However,further research is warranted to fully explore the diverse applications of OPN mucilage as a structural and functional component in foods.展开更多
文摘The present work focuses on the proximate physical and chemical profile of <em>Opuntia ficus-indica</em> mucilage, mechanically extracted from cladodes, a waste of pruning in traditional organic cactus pear orchards in Italy (San Cono, Sicily). The mechanical extraction increased the mucilage yield to 30% dry weight. Physical characterization concerns pH, viscosity, free acidity and density, useful for emulsifying capacity. Spectrophotometric analysis was applied to assess total carbohydrates, proteins, uronic acids, total polyphenols content and antioxidant capacity. DART-MS and SEM-EDX were performed to evaluate functional mucilage components and relative amounts of minerals, respectively. From the main results <em>Opuntia ficus-indica</em> by-product, in addition to the preponderant total carbohydrates content, shows the high concentration in calcium and potassium and a fair amount of health-promoting phytochemicals, which make it a good candidate for the different type of industrial applications.
基金supported by grants project from São Paulo Research Foundation(FAPESP)[project number:2022/016069]National Council for Scientific and Technological Development(CNPq-Brazil).
文摘The mucilage fraction from Ora-pro-Nobis(OPN)leaves was extracted and characterized regarding its chemical composition,rheological behavior,and prebiotic potential.The extraction involved hot water treatment followed by ethanol precipitation,resulting in a substantial extraction yield(4.77%of total leaves).This mucilage extraction served as an initial step for subsequent protein extraction from residual leaf material.OPN mucilage primarily comprises a high molecular weight polysaccharide(arabinogalactan),which upon hydrolysis,yields xylose(30.11%w/w)and arabinose(29.15%w/w)as primary components,alongside minor amounts of glucose(5.28%w/w)and cellobiose(3.80%w/w).Rheological analysis indicated pseudoplastic behavior and thixotropic properties,offering potential value in formulating 3D printed food products.Furthermore,di-gestibility analysis demonstrated that the mucilage was highly resistant to simulated gastrointestinal conditions,with only 2.69%degradation observed.In addition,assays evaluating its prebiotic potential showed that the mucilage supported the growth of the probiotic strain Lactobacillus acidophilus.Furthermore.All these findings,combined with the classification of glucoarabinoxylan as a dietary or functional fiber,underscore the potential of OPN mucilage as an innovative ingredient for food product development,promoting microbiota balance.However,further research is warranted to fully explore the diverse applications of OPN mucilage as a structural and functional component in foods.