This study analyzed the mitochondrial DNA D-loop hypervariable region 601 bp sequence in 12 Eutamias sibiricus from Heilongjiang area. The result showed that the average contents of A, T, G and C were 33.2%, 30.5%, 11...This study analyzed the mitochondrial DNA D-loop hypervariable region 601 bp sequence in 12 Eutamias sibiricus from Heilongjiang area. The result showed that the average contents of A, T, G and C were 33.2%, 30.5%, 11.8% and 24.5% respectively, the A＋T content （63.7%） was obviously higher than the G＋C content （36.3%）. Thirty-six, mutation （approximately 6.0%） sites were found and 9 haplotypes were defined. The mutations types, including transition, transversion and deletion were all found in the detected mtDNA D-loop regions, most of which was transition. The average nucleotide mutational ratio was 1.22%. The nucleotide mutation sites affected the restriction site appearance or disappearance of the restriction site. The research on mtDNA D-loop is focused on the domestic animals and there is no report on Eutamias sibiricus, This study analyzed the mitochondrial DNA D-loop hypervariable in Eutamias sibiricus so as to provide some useful informations for related research in the future.展开更多
Objective To explore the relationships intergenic region in mtDNA with PCOS.of variations in D-loop and COH-tRNA^Lys Methods A total of 77 PCOS and 45 non-PCOS patients were enrolled,whose D-loop and COH-tRNA^Lys inte...Objective To explore the relationships intergenic region in mtDNA with PCOS.of variations in D-loop and COH-tRNA^Lys Methods A total of 77 PCOS and 45 non-PCOS patients were enrolled,whose D-loop and COH-tRNA^Lys intergenic region in mtDNA were amplified and sequenced;sexual hormone assay,oral glucose tolerance test(OGTT)and insulin releasing test were carried out.Then variations found in mtDNA were compared between the two groups,the correlations between variations and clinical indexes were analyzed in all subjects.Results Nucleotide variations found in mtDNA were not different between the two groups,but the mutation of 16094T/C was found associated with the serum levels ofT and fasting insulin;(303-317)CnTC,associated with the serum levels of A and LH;195C/T with A level and 491T/C with LH level;(8272-8289)(ACCCCCTCT),was associated with the serum level of 1 h glucose.Conclusion Noncoding region mutations in mtDNA perhaps associate with PCOS clinical symptoms and involve in PCOS development.展开更多
Complete mitochondrial D-loop sequences of 140 samples obtained from 15 Chinese local cattle breeds were analyzed.The result showed 67 different haplotypes with 113 polymorphic sites.The haplotype diversity and nucleo...Complete mitochondrial D-loop sequences of 140 samples obtained from 15 Chinese local cattle breeds were analyzed.The result showed 67 different haplotypes with 113 polymorphic sites.The haplotype diversity and nucleotide diversity were 0.608-1.000 and 0.002-0.028,respectively,indicating abundant genetic diversity in Chinese cattle.The constructed Neighbor-Joining(NJ) tree revealed that there were two maternal origins of Bos taurus and Bos indicus in Chinese cattle.Among them,the Bos taurus lineage had rich mtDNA genetic diversity,while Bos indicus lineage had poor mtDNA genetic diversity,respectively.It can be concluded that the cattle in Northern China originated from the Bos taurus lineage,while the cattle in Central China and Southern China originated from Bos taurus and Bos indicus lineages.In our study,two mtDNA haplotypes belonging to African cattle were detected,thus providing molecular evidence for an African lineage in Chinese cattle.展开更多
文摘This study analyzed the mitochondrial DNA D-loop hypervariable region 601 bp sequence in 12 Eutamias sibiricus from Heilongjiang area. The result showed that the average contents of A, T, G and C were 33.2%, 30.5%, 11.8% and 24.5% respectively, the A＋T content （63.7%） was obviously higher than the G＋C content （36.3%）. Thirty-six, mutation （approximately 6.0%） sites were found and 9 haplotypes were defined. The mutations types, including transition, transversion and deletion were all found in the detected mtDNA D-loop regions, most of which was transition. The average nucleotide mutational ratio was 1.22%. The nucleotide mutation sites affected the restriction site appearance or disappearance of the restriction site. The research on mtDNA D-loop is focused on the domestic animals and there is no report on Eutamias sibiricus, This study analyzed the mitochondrial DNA D-loop hypervariable in Eutamias sibiricus so as to provide some useful informations for related research in the future.
基金supported by a grant from the Foundation of Ministry of Education of China(No.JHG06-7-03)
文摘Objective To explore the relationships intergenic region in mtDNA with PCOS.of variations in D-loop and COH-tRNA^Lys Methods A total of 77 PCOS and 45 non-PCOS patients were enrolled,whose D-loop and COH-tRNA^Lys intergenic region in mtDNA were amplified and sequenced;sexual hormone assay,oral glucose tolerance test(OGTT)and insulin releasing test were carried out.Then variations found in mtDNA were compared between the two groups,the correlations between variations and clinical indexes were analyzed in all subjects.Results Nucleotide variations found in mtDNA were not different between the two groups,but the mutation of 16094T/C was found associated with the serum levels ofT and fasting insulin;(303-317)CnTC,associated with the serum levels of A and LH;195C/T with A level and 491T/C with LH level;(8272-8289)(ACCCCCTCT),was associated with the serum level of 1 h glucose.Conclusion Noncoding region mutations in mtDNA perhaps associate with PCOS clinical symptoms and involve in PCOS development.
文摘Complete mitochondrial D-loop sequences of 140 samples obtained from 15 Chinese local cattle breeds were analyzed.The result showed 67 different haplotypes with 113 polymorphic sites.The haplotype diversity and nucleotide diversity were 0.608-1.000 and 0.002-0.028,respectively,indicating abundant genetic diversity in Chinese cattle.The constructed Neighbor-Joining(NJ) tree revealed that there were two maternal origins of Bos taurus and Bos indicus in Chinese cattle.Among them,the Bos taurus lineage had rich mtDNA genetic diversity,while Bos indicus lineage had poor mtDNA genetic diversity,respectively.It can be concluded that the cattle in Northern China originated from the Bos taurus lineage,while the cattle in Central China and Southern China originated from Bos taurus and Bos indicus lineages.In our study,two mtDNA haplotypes belonging to African cattle were detected,thus providing molecular evidence for an African lineage in Chinese cattle.
文摘为更好地对长顺绿壳蛋鸡进行品种保护和利用,并从母系遗传角度深入阐明长顺绿壳蛋鸡的群体遗传背景,利用PCR直接测序法,测定了114只长顺绿壳蛋鸡的线粒体DNA(mt DNA)D-loop第1高变区568 bp片段序列。结果表明:在所分析的长顺绿壳蛋鸡mt DNA D-loop区序列中,A、C、G、T平均比例为27.1%、29.5%、13.4%、30.0%;共检测到核苷酸变异位点37个,核苷酸多样度为0.0111,24种单倍型,单倍型多样度为0.845,表明长顺绿壳蛋鸡遗传多样性较丰富,具有较好的选育潜力。聚类分析结果表明,长顺绿壳蛋鸡与红原鸡亲缘关系较近。
