The aim of this study was to evaluate immunohistochemically TRAIL (TNF-Related Apoptosis Inducing Ligand) expression in the periodontal ligament of rats’ molars considering its distribution pattern in the tension and...The aim of this study was to evaluate immunohistochemically TRAIL (TNF-Related Apoptosis Inducing Ligand) expression in the periodontal ligament of rats’ molars considering its distribution pattern in the tension and compressive regions of ligament during orthodontic movement. Sixteen Sprague-Dawley rats, weighing between 120 and 200 gwere used in the present study. Tooth movement was induced by placing elastic bands between the maxillary first and second molars. The elastic bands were left in place for 12 (time T1) and 24 (time T2) hours. After rats’ euthanasia, maxillae were dissected free and the sections were exposed to a primary rabbit polyclonal anti-TRAIL anti-body. The staining status was identified as either negative or positive by using 3,3’-diaminobenzidine (DAB) to develop the immunoreaction. Data were analyzed using ANOVA test and a post-hoc Dunn’s Multiple Comparison test. P-values of less than 0.05 were considered statistically significant. The staining for TRAIL in the tension side appeared darker (intensity of staining = 2) than in the compressive side (intensity of staining = 1) 12 h after the tooth movement. The stainings of both sides of PDL were observed to be up-regulated 24 h after the application of the force (tension side = 3, compression side = 2;P 0.05). TRAIL is expressed in the periodontal ligament of rats’ molars during experimental tooth movement both in the compression and in the tension sides. The latter showed the highest amount of TRAIL immunolabeling.展开更多
Slungshots can be induced by earthquake, causing heavy damage to people. Preliminary study of this problem was carried out. Firstly,?a mathematical model for describing the movement of slungshot was presented based on...Slungshots can be induced by earthquake, causing heavy damage to people. Preliminary study of this problem was carried out. Firstly,?a mathematical model for describing the movement of slungshot was presented based on the theories of rigid body kinematics and aerodynamics. The effects of air drag and the rotation of rock were considered. Secondly, the trail of movement and the velocity were studied based on the presented model, taking the slungshot recorded in the 5.12 earthquake in the Yingxiu County as example. Thirdly, the impact force of the slungshot on the ground was studied. This study provides a reference for the forecast and prevention of the slungshot hazard.展开更多
目的探讨眼动分析对轻度认知功能障碍的筛查价值。方法计算机检索Pubmed、Embase、Cochrane Library、EBSCO、OVID、中国生物医学文献数据库(CBM)、中国知网数据库(CNKI)、万方和维普数据库在2020年3月之前公开发表的使用眼动追踪对轻...目的探讨眼动分析对轻度认知功能障碍的筛查价值。方法计算机检索Pubmed、Embase、Cochrane Library、EBSCO、OVID、中国生物医学文献数据库(CBM)、中国知网数据库(CNKI)、万方和维普数据库在2020年3月之前公开发表的使用眼动追踪对轻度认知功能障碍患者进行筛查的诊断性试验研究,并对符合纳入标准的文献采用NOS质量评价工具进行质量评价,提取研究特征和眼动指标相关数据(眼跳反应时间和眼跳错误率),使用RevMan5.3Meta分析软件进行数据分析。结果最终纳入6篇诊断性试验研究,共计514例,按Petersen标准,其中219例被诊断为轻度认知功能障碍。其中6篇文献研究了反向眼跳间隙范式的反应时间(AST gap Rt),合并的效应值均差和95%CI为23.37、(4.51~42.22);4篇文献研究了反向眼跳间隙范式的错误率,合并的效应值均差和95%CI为15.50、(14.99~16.01),轻度认知功能障碍组与健康对照组差异具有统计学意义(P<0.001)。2篇文献研究了朝向眼跳间隙范式的反应时间(PST gap Rt),合并的效应值均差和95%CI为13.68、(-8.88~36.25),两组差异无统计学意义(P=0.230)。结论反向眼跳反应时间和反向眼跳错误率等眼动指标可以较好地区分轻度认知功能障碍患者和健康者,而朝向眼跳反应时间对轻度认知功能障碍患者的筛查意义不大。展开更多
Abducens nerve palsy（ANP） is commonly seen in patients with diabetes mellitus. The validity of acupuncture as a traditional Chinese medicine method in peripheral nerve repair is well established. However, its effica...Abducens nerve palsy（ANP） is commonly seen in patients with diabetes mellitus. The validity of acupuncture as a traditional Chinese medicine method in peripheral nerve repair is well established. However, its efficacy in randomized controlled trials remains unclear. Herein, we designed a protocol for a prospective, single-center, randomized controlled trial to investigate the effect of intraorbital electroacupuncture on diabetic ANP. We plan to recruit 60 patients with diabetic ANP, and randomly divide them into treatment and control groups. Patients in both groups will continue their glucose-lowering therapy. A neural nutrition drug will be given to both groups for six weeks. The treatment group will also receive intraorbital electroacupuncture therapy. We will assess efficacy of treatment, eyeball movement, diplopia deviation and the levels of fasting blood-glucose and glycosylated hemoglobin before treatment at 2, 4, and 6 weeks after treatment. The efficacy and recurrence will be investigated during follow-up（1 month after intervention）. This protocol was registered at Chinese Clinical Trial Registry on 16 January 2015（Chi CTR-IPR-15005836）. This study was approved by the Ethics Committee of First Affiliated Hospital of Harbin Medical University of China（approval number： 201452）. All protocols will be in accordance with Declaration of Helsinki, formulated by the World Medical Association. Written informed consent will be provided by participants. We envisage that the results of this clinical trial will provide evidence for promoting clinical use of this new therapy for management of ANP.展开更多
文摘The aim of this study was to evaluate immunohistochemically TRAIL (TNF-Related Apoptosis Inducing Ligand) expression in the periodontal ligament of rats’ molars considering its distribution pattern in the tension and compressive regions of ligament during orthodontic movement. Sixteen Sprague-Dawley rats, weighing between 120 and 200 gwere used in the present study. Tooth movement was induced by placing elastic bands between the maxillary first and second molars. The elastic bands were left in place for 12 (time T1) and 24 (time T2) hours. After rats’ euthanasia, maxillae were dissected free and the sections were exposed to a primary rabbit polyclonal anti-TRAIL anti-body. The staining status was identified as either negative or positive by using 3,3’-diaminobenzidine (DAB) to develop the immunoreaction. Data were analyzed using ANOVA test and a post-hoc Dunn’s Multiple Comparison test. P-values of less than 0.05 were considered statistically significant. The staining for TRAIL in the tension side appeared darker (intensity of staining = 2) than in the compressive side (intensity of staining = 1) 12 h after the tooth movement. The stainings of both sides of PDL were observed to be up-regulated 24 h after the application of the force (tension side = 3, compression side = 2;P 0.05). TRAIL is expressed in the periodontal ligament of rats’ molars during experimental tooth movement both in the compression and in the tension sides. The latter showed the highest amount of TRAIL immunolabeling.
文摘Slungshots can be induced by earthquake, causing heavy damage to people. Preliminary study of this problem was carried out. Firstly,?a mathematical model for describing the movement of slungshot was presented based on the theories of rigid body kinematics and aerodynamics. The effects of air drag and the rotation of rock were considered. Secondly, the trail of movement and the velocity were studied based on the presented model, taking the slungshot recorded in the 5.12 earthquake in the Yingxiu County as example. Thirdly, the impact force of the slungshot on the ground was studied. This study provides a reference for the forecast and prevention of the slungshot hazard.
文摘目的探讨眼动分析对轻度认知功能障碍的筛查价值。方法计算机检索Pubmed、Embase、Cochrane Library、EBSCO、OVID、中国生物医学文献数据库(CBM)、中国知网数据库(CNKI)、万方和维普数据库在2020年3月之前公开发表的使用眼动追踪对轻度认知功能障碍患者进行筛查的诊断性试验研究,并对符合纳入标准的文献采用NOS质量评价工具进行质量评价,提取研究特征和眼动指标相关数据(眼跳反应时间和眼跳错误率),使用RevMan5.3Meta分析软件进行数据分析。结果最终纳入6篇诊断性试验研究,共计514例,按Petersen标准,其中219例被诊断为轻度认知功能障碍。其中6篇文献研究了反向眼跳间隙范式的反应时间(AST gap Rt),合并的效应值均差和95%CI为23.37、(4.51~42.22);4篇文献研究了反向眼跳间隙范式的错误率,合并的效应值均差和95%CI为15.50、(14.99~16.01),轻度认知功能障碍组与健康对照组差异具有统计学意义(P<0.001)。2篇文献研究了朝向眼跳间隙范式的反应时间(PST gap Rt),合并的效应值均差和95%CI为13.68、(-8.88~36.25),两组差异无统计学意义(P=0.230)。结论反向眼跳反应时间和反向眼跳错误率等眼动指标可以较好地区分轻度认知功能障碍患者和健康者,而朝向眼跳反应时间对轻度认知功能障碍患者的筛查意义不大。
基金supported by the National Natural Science Foundation of China,No.81674052the Key Project of Natural Science Foundation of Heilongjiang Province of China,No.ZD201211a grant from the Project of Innovational Scientific Research of Harbin Medical University of China,No.2016LCZX49
文摘Abducens nerve palsy（ANP） is commonly seen in patients with diabetes mellitus. The validity of acupuncture as a traditional Chinese medicine method in peripheral nerve repair is well established. However, its efficacy in randomized controlled trials remains unclear. Herein, we designed a protocol for a prospective, single-center, randomized controlled trial to investigate the effect of intraorbital electroacupuncture on diabetic ANP. We plan to recruit 60 patients with diabetic ANP, and randomly divide them into treatment and control groups. Patients in both groups will continue their glucose-lowering therapy. A neural nutrition drug will be given to both groups for six weeks. The treatment group will also receive intraorbital electroacupuncture therapy. We will assess efficacy of treatment, eyeball movement, diplopia deviation and the levels of fasting blood-glucose and glycosylated hemoglobin before treatment at 2, 4, and 6 weeks after treatment. The efficacy and recurrence will be investigated during follow-up（1 month after intervention）. This protocol was registered at Chinese Clinical Trial Registry on 16 January 2015（Chi CTR-IPR-15005836）. This study was approved by the Ethics Committee of First Affiliated Hospital of Harbin Medical University of China（approval number： 201452）. All protocols will be in accordance with Declaration of Helsinki, formulated by the World Medical Association. Written informed consent will be provided by participants. We envisage that the results of this clinical trial will provide evidence for promoting clinical use of this new therapy for management of ANP.
