Superficial microbiota disturbances are common in dogs, with Malassezia pachydermatis yeasts often being involved. Topical products are recommended as a first-line management strategy;however, literature regarding spe...Superficial microbiota disturbances are common in dogs, with Malassezia pachydermatis yeasts often being involved. Topical products are recommended as a first-line management strategy;however, literature regarding specific topical protocols is still scarce. This study aimed to evaluate the performance of a protocol combining chlorhexidine and Ophytrium-containing products in reducing yeast counts in dogs with microscopic evidence of yeast imbalance and dermatological signs. Dogs were shampooed on Day 0 (D0) and then received eight mousse applications at two to three-day intervals. Followed-up parameters on D0, Day 7 (D7), and Day 21 (D21) were microscopic yeast counting, pruritus, and skin irritation scores. At D21, veterinarians’ and owners’ overall assessments were collected. Malassezia pachydermatis counts decreased at D7 (57.3% vs D0, p p p p < 0.001). Efficacy was described by all veterinarians from good to excellent, and the proposed procedures were evaluated as efficient and practical by the owners in 91.7% of the cases. The protocol using chlorhexidine and Ophytrium shampoo and mousse could be a useful option in dogs presenting imbalances in yeast cutaneous microbiota.展开更多
The aim of this study is to evaluate the influence of Tooth Mousse （TM） application, smear layer removal, and storage time on resin-dentin microtensile bond strength （pTBS）. Dentin specimens were divided into two ...The aim of this study is to evaluate the influence of Tooth Mousse （TM） application, smear layer removal, and storage time on resin-dentin microtensile bond strength （pTBS）. Dentin specimens were divided into two groups： （1） smear layer covered; （2） smear layer removed using 15% EDTA for 90 s. In each group, half the specimens were treated once with TM for 60 min. After bonding procedures using a two-step self-etching adhesive （Clearfil SE Bond （CSE）; Kuraray Medical, Tokyo, Japan）, an all-in-one adhesive （G-Bond （GB）; GC Corp, Tokyo, Japan）, and a total-etch adhesive （Adper Single Bond 2 （SB）; 3M ESPE, St. Paul, MN, USA）, the specimens were stored for 3 d or 6 months in deionized water at 37 ℃, and pTBS was tested and analyzed. With the exception of SB （no TM application） and GB, the pTBS was significantly increased for CSE and SB using EDTA pre-conditioning and 3 d of storage （P≤0.001）. Bond strength of GB decreased significantly when using EDTA （3 d storage, P〈0.05）. TM application only increased the pTBS of GB （no EDTA） and SB （with EDTA） after 3 d （P≤0.02）. Comparing the adhesives after 3 d of storage, CSE exhibited the greatest pTBS values followed by GB and SB （P≤0.02）. The factors of adhesive, EDTA, and TM did not show any significant impact on pTBS when specimens were stored for 6 months （P〉0.05）. The additional application of TM and EDTA for cavity preparation seems only to have a short-term effect, and no influence on pTBS of dentin bonds after a period of 6 months.展开更多
文摘Superficial microbiota disturbances are common in dogs, with Malassezia pachydermatis yeasts often being involved. Topical products are recommended as a first-line management strategy;however, literature regarding specific topical protocols is still scarce. This study aimed to evaluate the performance of a protocol combining chlorhexidine and Ophytrium-containing products in reducing yeast counts in dogs with microscopic evidence of yeast imbalance and dermatological signs. Dogs were shampooed on Day 0 (D0) and then received eight mousse applications at two to three-day intervals. Followed-up parameters on D0, Day 7 (D7), and Day 21 (D21) were microscopic yeast counting, pruritus, and skin irritation scores. At D21, veterinarians’ and owners’ overall assessments were collected. Malassezia pachydermatis counts decreased at D7 (57.3% vs D0, p p p p < 0.001). Efficacy was described by all veterinarians from good to excellent, and the proposed procedures were evaluated as efficient and practical by the owners in 91.7% of the cases. The protocol using chlorhexidine and Ophytrium shampoo and mousse could be a useful option in dogs presenting imbalances in yeast cutaneous microbiota.
基金Project supported by the National Natural Science Foundation of China(Nos.81271955 and 30973350)the Zhejiang Provincial Natural Science Foundation of China(No.Y2080338)
文摘The aim of this study is to evaluate the influence of Tooth Mousse （TM） application, smear layer removal, and storage time on resin-dentin microtensile bond strength （pTBS）. Dentin specimens were divided into two groups： （1） smear layer covered; （2） smear layer removed using 15% EDTA for 90 s. In each group, half the specimens were treated once with TM for 60 min. After bonding procedures using a two-step self-etching adhesive （Clearfil SE Bond （CSE）; Kuraray Medical, Tokyo, Japan）, an all-in-one adhesive （G-Bond （GB）; GC Corp, Tokyo, Japan）, and a total-etch adhesive （Adper Single Bond 2 （SB）; 3M ESPE, St. Paul, MN, USA）, the specimens were stored for 3 d or 6 months in deionized water at 37 ℃, and pTBS was tested and analyzed. With the exception of SB （no TM application） and GB, the pTBS was significantly increased for CSE and SB using EDTA pre-conditioning and 3 d of storage （P≤0.001）. Bond strength of GB decreased significantly when using EDTA （3 d storage, P〈0.05）. TM application only increased the pTBS of GB （no EDTA） and SB （with EDTA） after 3 d （P≤0.02）. Comparing the adhesives after 3 d of storage, CSE exhibited the greatest pTBS values followed by GB and SB （P≤0.02）. The factors of adhesive, EDTA, and TM did not show any significant impact on pTBS when specimens were stored for 6 months （P〉0.05）. The additional application of TM and EDTA for cavity preparation seems only to have a short-term effect, and no influence on pTBS of dentin bonds after a period of 6 months.
