The leaves of Bt (Bacillus thuringiensis) transgenic poplar (Populus nigra L.) and CpTI (Cowpea trypsin inhibitor) transgenic poplar ((P. tomentosa×P. bolleana)×P. Tomentosa) were taken to feed the 4th-5th-i...The leaves of Bt (Bacillus thuringiensis) transgenic poplar (Populus nigra L.) and CpTI (Cowpea trypsin inhibitor) transgenic poplar ((P. tomentosa×P. bolleana)×P. Tomentosa) were taken to feed the 4th-5th-instar larvae of American white moth (Hyphantria cunea (Drury)) for determination of the activities of the protective enzyme system inside larvae’s body. The physiological and biochemical effects of the transgenic poplars on the larvae were studied. The results showed that the two kinds of transgenic poplars had similar effects on the protective enzyme system in the midgut of larvae. The activities of superoxide dismutase, catalase, and peroxidase in midgut of the larvae increased gradually, reached the highest value at a certain time, and then decreased suddenly. For the larvae that were fed with the leaves of Bt transgenic poplar, the peak value of superoxide dismutase and catalase presented at the time of 24-h feeding, while the peak of peroxidase took place at the time of 12-h feeding. The activities of these protective enzymes for the larvae that were fed with leaves of CpTI transgenic poplar peaked 12 h later than that of those fed with leaves of Bt transgenic poplar. The comparison of activities of the protective enzymes was also carried out between the larvae with different levels of intoxication. It was found that the activities of protective enzyme of the seriously intoxicant larvae were higher than that of the lightly intoxicant larvae. This difference was more obvious in the group treated with CpTI transgenic poplar.展开更多
Peeling trunk,binding insect-attracting belt,cleaning orchard and soil-covering under the crown were the control methods on overwintering generation of oriental fruit moth.However,they had not been applied widely on t...Peeling trunk,binding insect-attracting belt,cleaning orchard and soil-covering under the crown were the control methods on overwintering generation of oriental fruit moth.However,they had not been applied widely on the production because of the lack of systematic quantitative evaluation.Aiming at this fact,the control effects of these technical measures were studied in peach with different ripening period.The results showed that peeling trunk was the best with the control effect of88.64%.The control effect of binding insect-attracting belt of grass bundle was74.13%,which was the most economical and efficient.Covering with soil layer of 3cm under the crown during the middle ten days of March could holdback the adult getting out from soil.Cleaning deadwood could clean out the overwintering larvae on the ground.展开更多
American white moth is a remarkable worldwide quarantine pest. By the results combination of indoor incubation and field observation of American white moth in Langfang City of Hebei Province, series prevention and con...American white moth is a remarkable worldwide quarantine pest. By the results combination of indoor incubation and field observation of American white moth in Langfang City of Hebei Province, series prevention and control bottleneck factors for the invasive agricultural pest are analyzed, such as the main operation mode of the pest against host trees, diffusion and migration charaeteristies, biological characteristics, natural enemy control, pesticide prevention and so on. The re- search aims to search for the breakthrough point of the development of environment-friendly control techniques against American white moth, which also provides the reference for further improvement of integrated pest management system.展开更多
The model for predicting vegetable pest diamondback moth was established based on E-Support Vector Regression algorithms in the multiply occurrence season of diamondback moth. The experimental data of diamondback moth...The model for predicting vegetable pest diamondback moth was established based on E-Support Vector Regression algorithms in the multiply occurrence season of diamondback moth. The experimental data of diamondback moth in Guangdong vegetable were analyzed, and the result showed that when penalty factor c was 43, kernel function parameter k was O. 2, the better prediction result could be obtained by the early warning model of E-Support Vector Regression algorithms.展开更多
[ Objective] The paper was to study the parasitic ability and field control effect of Pyemotes sp. against oriental fruit moth （Grapholitha molesta Bussck） which caused damage on Photinia. [ Method] The indoor infec...[ Objective] The paper was to study the parasitic ability and field control effect of Pyemotes sp. against oriental fruit moth （Grapholitha molesta Bussck） which caused damage on Photinia. [ Method] The indoor infection test and field control test of Pyemotes sp. against oriental fruit moth was carried out. The parasitic ability and activity characteristic of Pyemotes sp. were observed, and the effect of environmental factors such as humidity and temperature on the para- sitic ability of Pyemotes sp. was studied. [Result] Pyemotes sp. had good control effect on oriental fruit moth under the proper conditions; the host searching abili- ties of Pyemotes sp. were different at different temperature and humidity conditions, humidity had large impact on the activity of Pyemotes sp., while temperature had large impact on the individual activity. [ Conclusion] The result showed that Pyemotes sp. had effective control effort on oriental fruit moth.展开更多
The genus Pseudancylis Horak is reported for the first time from China. P. acrogypsa （Turner, 1916） is described as new to China based on specimens from Guangxi Zhuang Autonomous Region. Images of the adults and the...The genus Pseudancylis Horak is reported for the first time from China. P. acrogypsa （Turner, 1916） is described as new to China based on specimens from Guangxi Zhuang Autonomous Region. Images of the adults and the genital structures ofP. acrogypsa are provided. A key to the species ofPseudancylis Horak and a world checklist is presented.展开更多
The tea geometridEctropis obliquais one of the most serious leaf-feeding insect pests in tea （Camelia sinensis） in East Asia. Although several volatile chemicals emitted from tea plants have been reported to be attr...The tea geometridEctropis obliquais one of the most serious leaf-feeding insect pests in tea （Camelia sinensis） in East Asia. Although several volatile chemicals emitted from tea plants have been reported to be attractive toE. obliqua moths, no synthetic attractants for E. obliqua moths have been developed. By measuring the behavioral responses of the moth to a series of chemicals in the lab, we found that a blend containing a ternary mixture containing （Z）-3-hexenal, （Z）-3-hexenyl hexanoate and benzyl alcohol clearly attracted toE. obliqua moths of both sex and that （Z）-3-hexenyl acetate could enhance the attractiveness of the ternary blend. Moreover, we found that the volatiles emitted from the plant-E. obliqua larva com-plex have the same attractiveness as： 1） the blend of volatiles containing the ternary mixture and 2） the blend containing （Z）-3-hexenyl acetate plus the ternary mixture to both male and female moths. In a ifeld bioassay, more male moths were observed on traps that were baited with the blend containing （Z）-3-hexenyl acetate plus the ternary mixture than on control traps. Our study raises the tantalizing possibility that synthetic blends could be deployed as attractants for pests in the ifeld.展开更多
To obtain transgenic cabbage line with broad insect resistance, a new synthetic Bacillus thuringiensis crylBa3 gene was introduced into white cabbage via Agrobacterium tumefaciens-mediated transformation and 37 transf...To obtain transgenic cabbage line with broad insect resistance, a new synthetic Bacillus thuringiensis crylBa3 gene was introduced into white cabbage via Agrobacterium tumefaciens-mediated transformation and 37 transformants were obtained. Polymerase chain reaction （PCR） and Southern blot analyses confirmed that crylBa3 was successfully inserted into the genome of cabbage. Reverse transcription-polymerase chain reaction （RT-PCR） demonstrated that crylBa3 was expressed. Western blot results confirmed the production of insecticidal protein encoded by crylBa3. Insect bioassays showed that transgenic cabbages effectively controlled both susceptible and CrylAc-resistant diamondback moth （DBM） larvae.展开更多
The construction of a pest detection algorithm is an important step to couple"ground-space"characteristics,which is also the basis for rapid and accurate monitoring and detection of pest damage.In four exper...The construction of a pest detection algorithm is an important step to couple"ground-space"characteristics,which is also the basis for rapid and accurate monitoring and detection of pest damage.In four experimental areas in Sanming City,Jiangle County,Sha County and Yanping District in Fujian Province,sample data on pest damage in 182 sets of Dendrolimus punctatus were collected.The data were randomly divided into a training set and testing set,and five duplicate tests and one eliminating-indicator test were done.Based on the characterization analysis of the host for D.punctatus damage,seven characteristic indicators of ground and remote sensing including leaf area index,standard error of leaf area index(SEL)of pine forest,normalized difference vegetation index(NDVI),wetness from tasseled cap transformation(WET),green band(B2),red band(B3),near-infrared band(B4)of remote sensing image are obtained to construct BP neural networks and random forest models of pest levels.The detection results of these two algorithms were comprehensively compared from the aspects of detection precision,kappa coefficient,receiver operating characteristic curve,and a paired t test.The results showed that the seven indicators all were responsive to pest damage,and NDVI was relatively weak;the average pest damage detection precision of six tests by BP neural networks was 77.29%,the kappa coefficient was 0.6869 and after the RF algorithm,the respective values were 79.30%and 0.7151,showing that the latter is more optimized,but there was no significant difference(p>0.05);the detection precision,kappa coefficient and AUC of the RF algorithm was higher than the BP neural networks for three pest levels(no damage,moderate damage and severe damage).The detection precision and AUC of BP neural networks were a little higher for mild damage,but the difference was not significant(p>0.05)except for the kappa coefficient for the no damage level(p<0.05).An"over-fitting"phenomenon tends to occur in BP neural networks,while RF method is more robust,providing a detection effect that is better than the BP neural networks.Thus,the application of the random forest algorithm for pest damage and multilevel dispersed variables is thus feasible and suggests that attention to the proportionality of sample data from various categories is needed when collecting data.展开更多
Arthropods and pathogens constantly challenge potato Solanum tuberosum L.production.Scenarios of climate variation have increased the possibility of changes in pests’biological and ecological patterns by increasing o...Arthropods and pathogens constantly challenge potato Solanum tuberosum L.production.Scenarios of climate variation have increased the possibility of changes in pests’biological and ecological patterns by increasing or reducing overwintering length,changes in population growth rates,number of generations,crop-pest relationship,and therefore affecting their expansion.Phthorimaea operculella Zeller(Lepidoptera:Gelechiidae)is one of the main pests affecting potatoes worldwide.Adults oviposit single or multiple eggs in leaves,stems,and tubers,while the larvae in immature stage mine leaves or burrows into tubers turning them unmarketable.Traditional control methods are effective in controlling P.operculella,but many factors determine the success of the control chosen.This review provides key highlights of current information available that could be used as a resource to fight this pest.展开更多
The potato tuber moth(PTM),Phthorimaea operculella(Zeller),is one of the most economically significant insect pests for potato in both field and storage worldwide.To evaluate the infestation,reduction of potato yield ...The potato tuber moth(PTM),Phthorimaea operculella(Zeller),is one of the most economically significant insect pests for potato in both field and storage worldwide.To evaluate the infestation,reduction of potato yield and the control efficacy for PTM,field tests were conducted in two seasons by intercropping of potato as the host plant with maize as a non-host plant of PTM.Three intercropping patterns were tested,which were 2 rows of potatoes with either 2,3,or 4 rows of maize(abbreviated 2 P:2 M,2 P:3 M,and 2 P:4 M),and the monocropped potato as the control,2 rows of potatoes,without maize,(abbreviated 2 P:0 M).Results showed that the population and infestation of PTM in the 2 P:3 M intercropping pattern was significantly lower than those in 2 P:2 M,2 P:4 M and the monocropping pattern of 2 P:0 M,due to the enhancement of natural enemies.Cumulative mines and tunneling in potato leaves in 2 P:3 M intercropping were significantly lower than those in 2 P:2 M and 2 P:4 M patterns.The population of parasitoids and the parasitism rate of PTM in intercropping pattern of 2 P:3 M were significantly higher than that in intercropping pattern of 2 P:2 M,2 P:4 M and monocropping pattern of 2 P:0 M.We conclude that the potato intercropped with maize reduced the adult and larva populations,and reduced the damage from PTM by enhancing the number of parasitoids and the level of parasitism.The greatest population density of parasitoids and parasitism rate were in the intercropping pattern of 2 rows of potatoes with 3 rows of maize.These data indicate that the host/non-host intercropping patterns can be used as a biological control tactic against PTM by enhancing the density of natural enemies in the agro-ecosystems.展开更多
The toxicities of fenvalerate （20% EC） to the 3rd instar larvae of diamondback moth （DBM）, Plutella xylostella （L.）, reared on three host plants viz., radish, oilseed rape, and cabbage were tested. The LC50 valu...The toxicities of fenvalerate （20% EC） to the 3rd instar larvae of diamondback moth （DBM）, Plutella xylostella （L.）, reared on three host plants viz., radish, oilseed rape, and cabbage were tested. The LC50 values of fenvalerate to the 3rd instar larvae of DBM varied with host plants, however, there wasn＇t any significant difference among them （P〉 0.05）. Similarly, DBM fed on three host plants had same pupal weight, pupal period, pupation rate, adult emergence rate, female ratio, and fecundity. The activity of juvenile hormone esterase （JHE, EC 3.1.1.1） in the 3rd instar larvae of DMB did not significantly vary with host plants, either. These suggested that DBM had similar fitness on the three host plant species. When fed on the host plants pretreated with fenvalerate at the concentrations equivalent to LC20, LC50 and LC50, the pupation rate, pupal weight, adult emergence rate, female ratio, fecundity, and JHE activity of the tested insects were declined as compared with insects in control treatments fed on the same host plant species. Furthermore, the pupal period of the tested insects was extended after fenvalerate treatment. The decrease in JHE activity after fenvalerate treatment in the tested insects could partly explain the changes in the mentioned growth parameters. Whether the role of fenvalerate in the inhibition of JHE activity could serve as a new way to control DBM needs further investigation.展开更多
Conogethes punctiferalis（Guenée）（Lepidoptera： Crambidae） was originally considered as one species with fruit-feeding type（FFT） and pinaceae-feeding type（PFT）, but it has subsequently been divided into tw...Conogethes punctiferalis（Guenée）（Lepidoptera： Crambidae） was originally considered as one species with fruit-feeding type（FFT） and pinaceae-feeding type（PFT）, but it has subsequently been divided into two different species of Conogethes punctiferalis and Conogethes pinicolalis. The relationship between the two species was investigated by phylogenetic reconstruction using maximum-likelihood（ML） parameter estimations. The phylogenetic tree and network were constructed based upon sequence data from concatenation of three genes of mitochondrial cytochrome c oxidase subunits I, II and cytochrome b which were derived from 118 samples of C. punctiferalis and 24 samples of C. pinicolalis. The phylogenetic tree and network showed that conspecific sequences were clustering together despite intraspecific variability. Here we report the results of a combined analysis of mitochondrial DNA sequences from three genes and morphological data representing powerful evidence that C. pinicolalisand C. punctiferalis are significantly different.展开更多
The combinative rate measurement of （3-[I125] iodotyrosyl） α-bungarotoxin was applied in the analysis of the relation between nerve acetylcholine receptor and three types of insecticide resistance in diamondback mo...The combinative rate measurement of （3-[I125] iodotyrosyl） α-bungarotoxin was applied in the analysis of the relation between nerve acetylcholine receptor and three types of insecticide resistance in diamondback moth, Plutella xylostella （L.）. In the dimehypo-resistant strain and in the cartap-resistant strain, the nerve acetylcholine receptor showed the remarkable insensitivity to dimehypo and cartap, of which the binding rate to ligand was approximately 66 and 60%, respectively, of the susceptible strain. The sensitivity to deltamethrin in the deltamethrin-resistant strain did not show visible change. These results indicated that the decline in the sensitivity of nerve acetylcholine receptor to insecticide might be a potential mechanism to nereistoxin insecticides resistance in the diamondback moth.展开更多
文摘The leaves of Bt (Bacillus thuringiensis) transgenic poplar (Populus nigra L.) and CpTI (Cowpea trypsin inhibitor) transgenic poplar ((P. tomentosa×P. bolleana)×P. Tomentosa) were taken to feed the 4th-5th-instar larvae of American white moth (Hyphantria cunea (Drury)) for determination of the activities of the protective enzyme system inside larvae’s body. The physiological and biochemical effects of the transgenic poplars on the larvae were studied. The results showed that the two kinds of transgenic poplars had similar effects on the protective enzyme system in the midgut of larvae. The activities of superoxide dismutase, catalase, and peroxidase in midgut of the larvae increased gradually, reached the highest value at a certain time, and then decreased suddenly. For the larvae that were fed with the leaves of Bt transgenic poplar, the peak value of superoxide dismutase and catalase presented at the time of 24-h feeding, while the peak of peroxidase took place at the time of 12-h feeding. The activities of these protective enzymes for the larvae that were fed with leaves of CpTI transgenic poplar peaked 12 h later than that of those fed with leaves of Bt transgenic poplar. The comparison of activities of the protective enzymes was also carried out between the larvae with different levels of intoxication. It was found that the activities of protective enzyme of the seriously intoxicant larvae were higher than that of the lightly intoxicant larvae. This difference was more obvious in the group treated with CpTI transgenic poplar.
文摘Peeling trunk,binding insect-attracting belt,cleaning orchard and soil-covering under the crown were the control methods on overwintering generation of oriental fruit moth.However,they had not been applied widely on the production because of the lack of systematic quantitative evaluation.Aiming at this fact,the control effects of these technical measures were studied in peach with different ripening period.The results showed that peeling trunk was the best with the control effect of88.64%.The control effect of binding insect-attracting belt of grass bundle was74.13%,which was the most economical and efficient.Covering with soil layer of 3cm under the crown during the middle ten days of March could holdback the adult getting out from soil.Cleaning deadwood could clean out the overwintering larvae on the ground.
基金Supported by Science and Technology Planning Project in Langfang City(2009012009)~~
文摘American white moth is a remarkable worldwide quarantine pest. By the results combination of indoor incubation and field observation of American white moth in Langfang City of Hebei Province, series prevention and control bottleneck factors for the invasive agricultural pest are analyzed, such as the main operation mode of the pest against host trees, diffusion and migration charaeteristies, biological characteristics, natural enemy control, pesticide prevention and so on. The re- search aims to search for the breakthrough point of the development of environment-friendly control techniques against American white moth, which also provides the reference for further improvement of integrated pest management system.
基金Supported by Science and Technology Projects in Guangdong Province(2009CD058,2009CD078,2009CD079,2009CD080)~~
文摘The model for predicting vegetable pest diamondback moth was established based on E-Support Vector Regression algorithms in the multiply occurrence season of diamondback moth. The experimental data of diamondback moth in Guangdong vegetable were analyzed, and the result showed that when penalty factor c was 43, kernel function parameter k was O. 2, the better prediction result could be obtained by the early warning model of E-Support Vector Regression algorithms.
基金Supported by Wuhan Landscape Gardening Bureau Project(Wu Park[2006]No.49)~~
文摘[ Objective] The paper was to study the parasitic ability and field control effect of Pyemotes sp. against oriental fruit moth （Grapholitha molesta Bussck） which caused damage on Photinia. [ Method] The indoor infection test and field control test of Pyemotes sp. against oriental fruit moth was carried out. The parasitic ability and activity characteristic of Pyemotes sp. were observed, and the effect of environmental factors such as humidity and temperature on the para- sitic ability of Pyemotes sp. was studied. [Result] Pyemotes sp. had good control effect on oriental fruit moth under the proper conditions; the host searching abili- ties of Pyemotes sp. were different at different temperature and humidity conditions, humidity had large impact on the activity of Pyemotes sp., while temperature had large impact on the individual activity. [ Conclusion] The result showed that Pyemotes sp. had effective control effort on oriental fruit moth.
基金supported by the National Natural Science Foundation of China(31101665)Beijing Higher Education Young Elite Teacher Project(YETP1716)
文摘The genus Pseudancylis Horak is reported for the first time from China. P. acrogypsa （Turner, 1916） is described as new to China based on specimens from Guangxi Zhuang Autonomous Region. Images of the adults and the genital structures ofP. acrogypsa are provided. A key to the species ofPseudancylis Horak and a world checklist is presented.
基金sponsored by the Special Fund for Agro-scientific Research in the Public Interest, China (201403030)the National Basic Research Program of China (2012CB114104)+1 种基金the National Natural Science Foundation of China (31272053)the Division of Science and Technology of Zhejiang Province, China (2015C32081)
文摘The tea geometridEctropis obliquais one of the most serious leaf-feeding insect pests in tea （Camelia sinensis） in East Asia. Although several volatile chemicals emitted from tea plants have been reported to be attractive toE. obliqua moths, no synthetic attractants for E. obliqua moths have been developed. By measuring the behavioral responses of the moth to a series of chemicals in the lab, we found that a blend containing a ternary mixture containing （Z）-3-hexenal, （Z）-3-hexenyl hexanoate and benzyl alcohol clearly attracted toE. obliqua moths of both sex and that （Z）-3-hexenyl acetate could enhance the attractiveness of the ternary blend. Moreover, we found that the volatiles emitted from the plant-E. obliqua larva com-plex have the same attractiveness as： 1） the blend of volatiles containing the ternary mixture and 2） the blend containing （Z）-3-hexenyl acetate plus the ternary mixture to both male and female moths. In a ifeld bioassay, more male moths were observed on traps that were baited with the blend containing （Z）-3-hexenyl acetate plus the ternary mixture than on control traps. Our study raises the tantalizing possibility that synthetic blends could be deployed as attractants for pests in the ifeld.
基金supported by the grants from the National High Technology Researchand Development Program of China (863 Program,2008AA10Z155)the National Natural Science Foundation of China (31071697)the earmarked fund for the Modern Agro-Industry Technology Research System,China (nycytx-35-gw01)
文摘To obtain transgenic cabbage line with broad insect resistance, a new synthetic Bacillus thuringiensis crylBa3 gene was introduced into white cabbage via Agrobacterium tumefaciens-mediated transformation and 37 transformants were obtained. Polymerase chain reaction （PCR） and Southern blot analyses confirmed that crylBa3 was successfully inserted into the genome of cabbage. Reverse transcription-polymerase chain reaction （RT-PCR） demonstrated that crylBa3 was expressed. Western blot results confirmed the production of insecticidal protein encoded by crylBa3. Insect bioassays showed that transgenic cabbages effectively controlled both susceptible and CrylAc-resistant diamondback moth （DBM） larvae.
基金supported by the National Natural Science Foundation of China(Grant Nos.41501361,41401385,30871965)the China Postdoctoral Science Foundation(No.2018M630728)+2 种基金the Open Fund of Fujian Provincial Key Laboratory of Resources and Environment Monitoring&Sustainable Management and Utilization(No.ZD1403)the Open Fund of Fujian Mine Ecological Restoration Engineering Technology Research Center(No.KS2018005)the Scientific Research Foundation of Fuzhou University(No.XRC1345)
文摘The construction of a pest detection algorithm is an important step to couple"ground-space"characteristics,which is also the basis for rapid and accurate monitoring and detection of pest damage.In four experimental areas in Sanming City,Jiangle County,Sha County and Yanping District in Fujian Province,sample data on pest damage in 182 sets of Dendrolimus punctatus were collected.The data were randomly divided into a training set and testing set,and five duplicate tests and one eliminating-indicator test were done.Based on the characterization analysis of the host for D.punctatus damage,seven characteristic indicators of ground and remote sensing including leaf area index,standard error of leaf area index(SEL)of pine forest,normalized difference vegetation index(NDVI),wetness from tasseled cap transformation(WET),green band(B2),red band(B3),near-infrared band(B4)of remote sensing image are obtained to construct BP neural networks and random forest models of pest levels.The detection results of these two algorithms were comprehensively compared from the aspects of detection precision,kappa coefficient,receiver operating characteristic curve,and a paired t test.The results showed that the seven indicators all were responsive to pest damage,and NDVI was relatively weak;the average pest damage detection precision of six tests by BP neural networks was 77.29%,the kappa coefficient was 0.6869 and after the RF algorithm,the respective values were 79.30%and 0.7151,showing that the latter is more optimized,but there was no significant difference(p>0.05);the detection precision,kappa coefficient and AUC of the RF algorithm was higher than the BP neural networks for three pest levels(no damage,moderate damage and severe damage).The detection precision and AUC of BP neural networks were a little higher for mild damage,but the difference was not significant(p>0.05)except for the kappa coefficient for the no damage level(p<0.05).An"over-fitting"phenomenon tends to occur in BP neural networks,while RF method is more robust,providing a detection effect that is better than the BP neural networks.Thus,the application of the random forest algorithm for pest damage and multilevel dispersed variables is thus feasible and suggests that attention to the proportionality of sample data from various categories is needed when collecting data.
文摘Arthropods and pathogens constantly challenge potato Solanum tuberosum L.production.Scenarios of climate variation have increased the possibility of changes in pests’biological and ecological patterns by increasing or reducing overwintering length,changes in population growth rates,number of generations,crop-pest relationship,and therefore affecting their expansion.Phthorimaea operculella Zeller(Lepidoptera:Gelechiidae)is one of the main pests affecting potatoes worldwide.Adults oviposit single or multiple eggs in leaves,stems,and tubers,while the larvae in immature stage mine leaves or burrows into tubers turning them unmarketable.Traditional control methods are effective in controlling P.operculella,but many factors determine the success of the control chosen.This review provides key highlights of current information available that could be used as a resource to fight this pest.
基金supported by the National Key Research and Development Program of China (2018YFD0200703 and 2018YFD0200802)the National Natural Science Foundation of China (3176059 and 31660537)
文摘The potato tuber moth(PTM),Phthorimaea operculella(Zeller),is one of the most economically significant insect pests for potato in both field and storage worldwide.To evaluate the infestation,reduction of potato yield and the control efficacy for PTM,field tests were conducted in two seasons by intercropping of potato as the host plant with maize as a non-host plant of PTM.Three intercropping patterns were tested,which were 2 rows of potatoes with either 2,3,or 4 rows of maize(abbreviated 2 P:2 M,2 P:3 M,and 2 P:4 M),and the monocropped potato as the control,2 rows of potatoes,without maize,(abbreviated 2 P:0 M).Results showed that the population and infestation of PTM in the 2 P:3 M intercropping pattern was significantly lower than those in 2 P:2 M,2 P:4 M and the monocropping pattern of 2 P:0 M,due to the enhancement of natural enemies.Cumulative mines and tunneling in potato leaves in 2 P:3 M intercropping were significantly lower than those in 2 P:2 M and 2 P:4 M patterns.The population of parasitoids and the parasitism rate of PTM in intercropping pattern of 2 P:3 M were significantly higher than that in intercropping pattern of 2 P:2 M,2 P:4 M and monocropping pattern of 2 P:0 M.We conclude that the potato intercropped with maize reduced the adult and larva populations,and reduced the damage from PTM by enhancing the number of parasitoids and the level of parasitism.The greatest population density of parasitoids and parasitism rate were in the intercropping pattern of 2 rows of potatoes with 3 rows of maize.These data indicate that the host/non-host intercropping patterns can be used as a biological control tactic against PTM by enhancing the density of natural enemies in the agro-ecosystems.
基金support for this work was provided by the National Natural Science Foundation of China(30971922)the Natural Science Foundation of Fujian Province, China (B0320003, B0410015, 2004J010, and2007F5021)+1 种基金the Science and Technology Innovation Foundation of Fujian Academy of Agricultural Science,China (STIF-Y07)the Program for New Century Excellent Talents in University of Fujian Province, China,to Associate Professor Gu Xiaojun
文摘The toxicities of fenvalerate （20% EC） to the 3rd instar larvae of diamondback moth （DBM）, Plutella xylostella （L.）, reared on three host plants viz., radish, oilseed rape, and cabbage were tested. The LC50 values of fenvalerate to the 3rd instar larvae of DBM varied with host plants, however, there wasn＇t any significant difference among them （P〉 0.05）. Similarly, DBM fed on three host plants had same pupal weight, pupal period, pupation rate, adult emergence rate, female ratio, and fecundity. The activity of juvenile hormone esterase （JHE, EC 3.1.1.1） in the 3rd instar larvae of DMB did not significantly vary with host plants, either. These suggested that DBM had similar fitness on the three host plant species. When fed on the host plants pretreated with fenvalerate at the concentrations equivalent to LC20, LC50 and LC50, the pupation rate, pupal weight, adult emergence rate, female ratio, fecundity, and JHE activity of the tested insects were declined as compared with insects in control treatments fed on the same host plant species. Furthermore, the pupal period of the tested insects was extended after fenvalerate treatment. The decrease in JHE activity after fenvalerate treatment in the tested insects could partly explain the changes in the mentioned growth parameters. Whether the role of fenvalerate in the inhibition of JHE activity could serve as a new way to control DBM needs further investigation.
基金supported by China Agriculture Research System(CARS-02)Beijing Municipal Sci-Tech Program(Z111100056811009)
文摘Conogethes punctiferalis（Guenée）（Lepidoptera： Crambidae） was originally considered as one species with fruit-feeding type（FFT） and pinaceae-feeding type（PFT）, but it has subsequently been divided into two different species of Conogethes punctiferalis and Conogethes pinicolalis. The relationship between the two species was investigated by phylogenetic reconstruction using maximum-likelihood（ML） parameter estimations. The phylogenetic tree and network were constructed based upon sequence data from concatenation of three genes of mitochondrial cytochrome c oxidase subunits I, II and cytochrome b which were derived from 118 samples of C. punctiferalis and 24 samples of C. pinicolalis. The phylogenetic tree and network showed that conspecific sequences were clustering together despite intraspecific variability. Here we report the results of a combined analysis of mitochondrial DNA sequences from three genes and morphological data representing powerful evidence that C. pinicolalisand C. punctiferalis are significantly different.
基金the National Natural Science Foundation of China （30160050）.
文摘The combinative rate measurement of （3-[I125] iodotyrosyl） α-bungarotoxin was applied in the analysis of the relation between nerve acetylcholine receptor and three types of insecticide resistance in diamondback moth, Plutella xylostella （L.）. In the dimehypo-resistant strain and in the cartap-resistant strain, the nerve acetylcholine receptor showed the remarkable insensitivity to dimehypo and cartap, of which the binding rate to ligand was approximately 66 and 60%, respectively, of the susceptible strain. The sensitivity to deltamethrin in the deltamethrin-resistant strain did not show visible change. These results indicated that the decline in the sensitivity of nerve acetylcholine receptor to insecticide might be a potential mechanism to nereistoxin insecticides resistance in the diamondback moth.
