Type A aortic dissection(AD)is one of the most serious cardiovascular diseases,whose risk predictors are controversial.The purpose of this research was to investigate how elongation accompanied by dilation of the asce...Type A aortic dissection(AD)is one of the most serious cardiovascular diseases,whose risk predictors are controversial.The purpose of this research was to investigate how elongation accompanied by dilation of the ascending aorta(AAo)affects the relevant haemodynamic characteristics using image-based computational models.Five elongated AAos with different levels of dilation have been reconstructed based on the centerlines data of an elderly and an AD patient.Numerical simulations have been performed assuming an inflow waveform and a Windkessel model with three elements for all outflow boundaries.The numerical results have revealed that the elongation of AAo can disturb the systolic helical flow pattern between the root of AAo and the aortic arch.The helical flow inside the AAo starts to develop into a vortex flow when the elongated AAo becomes dilated.The vortex gives rise to a localized oscillatory shear index at the ostia of the brachiocephalic artery(BA)and the inner curve of the aortic arch.This study suggests that abnormal growth of AAo,especially accompanied by its moderate dilation,can be considered as morphological risk factors of AD.展开更多
Phascolosoma esculenta is an intertidal organism that has recently attracted attention because of its ability to survive at relatively low temperatures.However,the gene regulation in P.esculenta in relation to its res...Phascolosoma esculenta is an intertidal organism that has recently attracted attention because of its ability to survive at relatively low temperatures.However,the gene regulation in P.esculenta in relation to its response to low temperatures is unclear.To explore the low temperature adaptability of P.esculenta,this study analyzed the changes in the morphology and hsp70 and hsp90 gene expression of P.esculenta exposed to a low temperature gradient.At 5℃,P.esculenta stretched and softened,and some individuals moved apart from the group.Histological analysis revealed cuticle breaches,myofiber scattering,disruption of the body wall,and epithelial layer dispersion and muscle fiber rupturing in the nephridium.Furthermore,the mRNA expression levels of hsp70 and hsp90 increased under acute low temperature stress,suggesting that these genes function in low temperature tolerance.Overall,low temperature stress causes morphological changes and histological damage in P.esculenta,and hsp70 and hsp90 potentially function in the low temperature adaptability of P.esculenta.Our results provide new insights into the adaptive strategies of P.esculenta under low temperature environments.展开更多
Cup-like nuclear morphological alterations in acute myeloid leukemia(AML)blasts have been widely correlated with Nucleophosmin 1(NPM1)mutations.NPM1-mutated AML has earned recognition as a distinct entity among myeloi...Cup-like nuclear morphological alterations in acute myeloid leukemia(AML)blasts have been widely correlated with Nucleophosmin 1(NPM1)mutations.NPM1-mutated AML has earned recognition as a distinct entity among myeloid tumors,but the absence of a thoroughly established tool for its morphological analysis remains a notable gap.Holographic tomography(HT)can offer a label-free solution for quantitatively assessing the 3D shape of the nucleus based on the volumetric variations of its refractive indices(RIs).However,traditional HT methods analyze adherent cells in a 2D layer,leading to non-isotropic reconstructions due to missing cone artifacts.Here we show for the first time that holo-tomographic flow cytometry(HTFC)achieves quantitative specificity and precise capture of the nucleus volumetric shape in AML cells in suspension.To retrieve nucleus specificity in label-free RI tomograms of flowing AML cells,we conceive and demonstrate in a real-world clinical case a novel strategy for segmenting 3D concave nuclei.This method implies that the correlation between the"phenotype"and"genotype"of nuclei is demonstrated through HTFC by creating a challenging link not yet explored between the aberrant morphological features of AML nuclei and NPM1 mutations.We conduct an ensemble-level statistical characterization of NPM1-wild type and NPM1-mutated blasts to discern their complex morphological and biophysical variances.Our findings suggest that characterizing cup-like nuclei in NPM1-related AML cells by HTFC may enhance the diagnostic approach for these tumors.Furthermore,we integrate virtual reality to provide an immersive fruition of morphological changes in AML cells within a true 3D environment.展开更多
基金the National Natural Science Foundation of China[No.51976026]Dalian Innovative Funding of Science and Technology[No.2018J12SN076]the Fundamental Research Funds for the Central Universities[DUT20GJ203].
文摘Type A aortic dissection(AD)is one of the most serious cardiovascular diseases,whose risk predictors are controversial.The purpose of this research was to investigate how elongation accompanied by dilation of the ascending aorta(AAo)affects the relevant haemodynamic characteristics using image-based computational models.Five elongated AAos with different levels of dilation have been reconstructed based on the centerlines data of an elderly and an AD patient.Numerical simulations have been performed assuming an inflow waveform and a Windkessel model with three elements for all outflow boundaries.The numerical results have revealed that the elongation of AAo can disturb the systolic helical flow pattern between the root of AAo and the aortic arch.The helical flow inside the AAo starts to develop into a vortex flow when the elongated AAo becomes dilated.The vortex gives rise to a localized oscillatory shear index at the ostia of the brachiocephalic artery(BA)and the inner curve of the aortic arch.This study suggests that abnormal growth of AAo,especially accompanied by its moderate dilation,can be considered as morphological risk factors of AD.
基金supported by the Ningbo Science and Technology Plan Projects(Nos.2019B10016,2016C10004)the Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture,the K.C.Wong Magna Fund in Ningbo University。
文摘Phascolosoma esculenta is an intertidal organism that has recently attracted attention because of its ability to survive at relatively low temperatures.However,the gene regulation in P.esculenta in relation to its response to low temperatures is unclear.To explore the low temperature adaptability of P.esculenta,this study analyzed the changes in the morphology and hsp70 and hsp90 gene expression of P.esculenta exposed to a low temperature gradient.At 5℃,P.esculenta stretched and softened,and some individuals moved apart from the group.Histological analysis revealed cuticle breaches,myofiber scattering,disruption of the body wall,and epithelial layer dispersion and muscle fiber rupturing in the nephridium.Furthermore,the mRNA expression levels of hsp70 and hsp90 increased under acute low temperature stress,suggesting that these genes function in low temperature tolerance.Overall,low temperature stress causes morphological changes and histological damage in P.esculenta,and hsp70 and hsp90 potentially function in the low temperature adaptability of P.esculenta.Our results provide new insights into the adaptive strategies of P.esculenta under low temperature environments.
基金supported by the project PRIN 2022 PNRR—flow-cytometry ImaGing by Holographic tomography for predicting TUMor control in Oncology patients treated with Radiotherapy(FIGHT-TUMOR),Prot.P2022ATE2J—funded by the Italian Ministry of University&Research in the framework of Next Generation EUby project“CITOM”—Programma AMICO 2,CNR—UVR—within the PoC 2022—PNRR funded by the Italian Ministry of Business and Made in Italy—UIBM in the framework of Next Generation EUby#NEXTGENERATIONEU(NGEU),Ministry of University and Research(MUR),National Recovery and Resilience Plan(NRRP),project MNESYS(PE0000006)—a multiscale integrated approach to the study of the nervous system in health and disease(DN.155311.10.2022)。
文摘Cup-like nuclear morphological alterations in acute myeloid leukemia(AML)blasts have been widely correlated with Nucleophosmin 1(NPM1)mutations.NPM1-mutated AML has earned recognition as a distinct entity among myeloid tumors,but the absence of a thoroughly established tool for its morphological analysis remains a notable gap.Holographic tomography(HT)can offer a label-free solution for quantitatively assessing the 3D shape of the nucleus based on the volumetric variations of its refractive indices(RIs).However,traditional HT methods analyze adherent cells in a 2D layer,leading to non-isotropic reconstructions due to missing cone artifacts.Here we show for the first time that holo-tomographic flow cytometry(HTFC)achieves quantitative specificity and precise capture of the nucleus volumetric shape in AML cells in suspension.To retrieve nucleus specificity in label-free RI tomograms of flowing AML cells,we conceive and demonstrate in a real-world clinical case a novel strategy for segmenting 3D concave nuclei.This method implies that the correlation between the"phenotype"and"genotype"of nuclei is demonstrated through HTFC by creating a challenging link not yet explored between the aberrant morphological features of AML nuclei and NPM1 mutations.We conduct an ensemble-level statistical characterization of NPM1-wild type and NPM1-mutated blasts to discern their complex morphological and biophysical variances.Our findings suggest that characterizing cup-like nuclei in NPM1-related AML cells by HTFC may enhance the diagnostic approach for these tumors.Furthermore,we integrate virtual reality to provide an immersive fruition of morphological changes in AML cells within a true 3D environment.
