Rheumatoid arthritis(RA)is a refractory autoimmune disease with limited treatment options.Plantderived exosomes-like nanovesicles(PDENs)have emerged as a novel nanomedical approach,with the inherent bioactive compound...Rheumatoid arthritis(RA)is a refractory autoimmune disease with limited treatment options.Plantderived exosomes-like nanovesicles(PDENs)have emerged as a novel nanomedical approach,with the inherent bioactive compounds from their source plants.The roots of Morinda officinalis How.(MO),a Chinese herb,exhibit notable anti-inflammatory activities and hold promising therapeutic value.We engineered a joint-targe ting delivery system(termed MOE@EM)by masking MO-derived exosomes-like nanovesicles(MOE)with erythrocyte membrane(EM).This biomimetic strategy,using EM camouflage,is intended to improve the in vivo fate of MOE.We investigated the antioxidative and anti-inflammatory activities,immunogenicity,drug accumulation in the joint,and therapeutic efficacy to ascertain its suitability for RA therapy.UV irradiation significantly increased the activities of catalase and peroxidase of MOE,and enhanced the anti-inflammatory effects via the Wnt/β-catenin pathway.Furthermore,MOE@EM markedly attenuated dendritic cell activation.MOE@EM exhibited joint-specific delivery,with substantial reduction in paw swelling,and favorable modulation of immune microenvironment.展开更多
Based on the meteorological data of 24 automatic stations in Xinfeng County,combined with the climatic conditions for the growth of Morinda officinalis F.C.How and Sarcandra glabra(Thunb.)Nakai,the climate suitability...Based on the meteorological data of 24 automatic stations in Xinfeng County,combined with the climatic conditions for the growth of Morinda officinalis F.C.How and Sarcandra glabra(Thunb.)Nakai,the climate suitability zoning indicators of M.officinalis and S.glabra in Xinfeng County were constructed by relevant statistical methods,and the climate suitability zoning indicators were divided into four grades:most suitable,suitable,sub-suitable and unsuitable.According to the results of expert scores,the relevant growth impact factors were assigned weight values.Based on geographic information system(GIS),the climate suitability zoning of M.officinalis and S.glabra planting in Xinfeng County was carried out.The results show that most areas of Xinfeng County were suitable for planting M.officinalis and S.glabra.Among them,the most suitable planting areas of M.officinalis were mainly distributed in most of Huilong Town,from Jiangnan Community of Fengcheng Street to Yuntianhai area of Meikeng Town,Matouwantian Village and areas to the south,and areas to the south of Zhutong Village,Fengcheng Street.The most suitable areas for S.glabra planting were mainly distributed in Puchang Village of Huilong Town,the northeast of Huangzhai Town,the south of Fengcheng Street,and the central-southern part of Matou Town.The zoning results provide a scientific basis for the rational layout of M.officinalis and S.glabra planting in Xinfeng County.展开更多
The fruit of Morinda citrifolia L., commonly known as noni, has an extensive history of use as a food and traditional medicine around the world. Adding value to Morinda citrifolia L. products, particularly the fruit, ...The fruit of Morinda citrifolia L., commonly known as noni, has an extensive history of use as a food and traditional medicine around the world. Adding value to Morinda citrifolia L. products, particularly the fruit, could be one way of building resilience in vulnerable farming households. The aim of this study was to determine the secondary metabolite and mineral composition of noni juice obtained by fermenting the fruit of Morinda citrifolia L. Fruits were collected in August 2022 from the local field in Thiès region, West of Senegal. Extraction yields were determined and the secondary metabolites were determined using conventional analytical methods. Calcium, magnesium, iron, sodium and potassium were determined by atomic absorption spectrophotometer coupled with a CCD detector. The results show that an average fruit mass (503.2 ± 110.96 g) consists of 171.44 ± 50.01 g pulp and 34.06 ± 10.35 g seeds. The traditional extraction yield of noni juice is 16.46% after three weeks of fermentation. The contents of total polyphenols, flavonoids and tannins obtained in noni are 608.97 ± 4.53 mg EAG/100mL, 7.78 ± 0.01 mg EQ/100mL and 0.191 ± 0.01 mg EC/100mL respectively. The ethanol content of noni varies from 3.57 to 5.23 mL/100mL during extraction. Noni has a high calcium content with a concentration of 383.79 ± 33.23 mg/L. This is followed by a good concentration of magnesium, potassium and sodium, at 278.47 ± 26.30, 187.43 ± 10.7 and 155.95 ± 28.66 mg/L respectively. Noni also has an iron content of 202.15 ± 0.05 mg/L.展开更多
Aim： To investigate the antioxidant effects of Morinda officinalis （Morindae radix, MR） on H2O2-induced oxidative stress in cultured mouse TM3 Leydig cells. Methods： We carried out 2,2-diphenyl-1-picrylhydrazyl fr...Aim： To investigate the antioxidant effects of Morinda officinalis （Morindae radix, MR） on H2O2-induced oxidative stress in cultured mouse TM3 Leydig cells. Methods： We carried out 2,2-diphenyl-1-picrylhydrazyl free radical scavenging, 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide, lipid peroxidation, testosterone enzyme immunoassay, superoxide dismutase （SOD）, and catalase （CAT） assays in Leydig TM3 cells. Results： MR showed a 47.8% 2,2-diphenyl-1-picrylhydrazyl radical scavenging effect in TM3 cells with no significant cytotoxicity. Oxidative stress was induced in TM3 cells with 100 μmol H2O2, and treatment of the cells with 250 μg/mL MR showed the most significant protective effect （64%, P 〈 0.001） in the cell viability assay with a decreased lipid peroxidation level （1.75 nmol/mg protein, P 〈 0.05）, increased testosterone production （43.5 pg/mL）, and improvements in SOD activity （7.49 units of SOD/mg protein, P 〈 0.001） and CAT activity （74.6 units of CAT/mg protein, P 〈 0.001）. Conclusion： These findings indicate that MR, as an antioxidant, protects functions of cultured mouse TM3 Leydig cells from H2O2-induced oxidative stress.展开更多
AIM:To explore the therapeutic effects of Morinda officinalis capsules(MOP) on osteoporosis in ovariectomized rats.METHOD:Six-month-old female Sprague–Dawley rats were induced for postmenopausal osteoporosis(PMOP) by...AIM:To explore the therapeutic effects of Morinda officinalis capsules(MOP) on osteoporosis in ovariectomized rats.METHOD:Six-month-old female Sprague–Dawley rats were induced for postmenopausal osteoporosis(PMOP) by bilateral ovariectomy and divided into seven groups as follows:sham-operated group,ovariectomized(OVX) control group,OVX treated with xianlinggubao(XLGB)(270 mg·kg-1·d-1),OVX treated with alendronate sodium(ALN)(3 mg·kg-1·d-1),and OVX treated with Morinda officinalis capsule(MOP) of graded doses(90,270 and 810 mg·kg-1·d-1) groups.Oral treatments were administered daily on the 4th week after ovariectomy and lasted for 12 weeks.The bone mineral density was evaluated by dual-energy X-ray absorptiometry.The tartrate-resistant acid phosphatase(TRAP),alkaline phosphatase(AKP),and osteocalcin(OC) levels in the serum and plasma were determined by standard colorimetric and enzyme immunoassays methods.Bone biomechanical properties and morphological parameters were analyzed by three-point bending test and histomorphometry respectively.RESULTS:Morinda officinalis capsules at all doses were able to significantly prevent the OVX-induced loss of bone mass due to diminishing serum AKP and TRAP levels while elevating OC level in the plasma.Morinda officinalis capsules also enhanced the bone strength and prevented the deterioration of trabecular microarchitecture.CONCLUSION:Morinda officinalis capsules possess potent anti-osteoporotic activity in OVX rats which could be an effective treatment for postmenopausal osteoporosis.展开更多
Objective:To evaluate the antioxidant and cytotoxic effects of hexane extract of Morinda pubescens leaves and to find the primary bioactive compound responsible for antioxidant and cytotoxic activities.Methods:The ind...Objective:To evaluate the antioxidant and cytotoxic effects of hexane extract of Morinda pubescens leaves and to find the primary bioactive compound responsible for antioxidant and cytotoxic activities.Methods:The individual compounds were isolated using column chromatography and were characterized by spectroscopic techniques.The antioxidant activity was evaluated for all individual isolated compounds by DPPH method using L-Ascorbic acid as standard and cytotoxicity was assessed for the extract and the hyoscyamine by MTT assay, caspase test and RT-PCR study.Results:The antioxidant activity of the isolated compounds and the extract increased as the concentration increased.One of the isolated compound hyoscyamine showed the high antioxidant activity.The extract and the hyoscyamine dose-dependently decreased the cell viability in HepG2 cells.Hyoscyamine induced caspase-3 mediated apoptosis. Up regulation of p53 gene expression provides cue for apoptotic activity of hyoscyamine. Conclusions:The results indicate that hexane extract possessed potent antioxidant and cytotoxic activity and hyoscyamine is the principal bioactive compound in hexane extract.展开更多
Extraction and characterization of antioxidative compositions from the extracts of fermented Xisha Noni （Morinda citrifolia L.） juice were studied. The antioxidative constituents of 184.6 g freeze-dried extracts of ...Extraction and characterization of antioxidative compositions from the extracts of fermented Xisha Noni （Morinda citrifolia L.） juice were studied. The antioxidative constituents of 184.6 g freeze-dried extracts of naturally fermented Xisha Noni juice were isolated successfully by petroleum ether, EtOAc and n-BuOH solvents, and the antioxidative effects were measured according to scavenging activity against hydroxyl generated in Fenton reaction system and superoxide anion radicals in pyrogallol autoxidation system. The EtOAc extract exhibited most significantly higher （P 〈 0.01） antioxidative activity than mannitol or vitamin C, while the petroleum ether and n-BuOH extracts showed lower activities compared to mannitol. Three antioxidant phenolic compounds, isoscopoletin, aesculetin and 3,3＇,4＇,5,7-pentahydroxyflavone （quercetin） were isolated from the EtOAc extract by several chromatography techniques for the first time. The results suggest that several compounds, in particular, the phenolic compounds, contribute separately or synergistically to the antioxidative activity of fermented Noni fruit juice.展开更多
The effect of Morinda tinctoria(MT) leaves extract on the corrosion inhibition of Al in acid medium was studied. The inhibition studies were carried out on Al in 0.5 mol/L HCl with the extract of leaves of MT using ma...The effect of Morinda tinctoria(MT) leaves extract on the corrosion inhibition of Al in acid medium was studied. The inhibition studies were carried out on Al in 0.5 mol/L HCl with the extract of leaves of MT using mass loss and electrochemical techniques. Parameters, such as concentration of the inhibitor, concentration of the acid, temperature and concentration of halide ions, were varied and optimized. Increase of the temperature and concentration of acid can decrease the performance of the inhibitor. Thermodynamic parameters show that the physisorption of the inhibitor molecules on Al surface obeys Langmuir isotherm.展开更多
Noni juice （Morinda citrifolia） has been approved for use as a safe food within the European Union, following a review of safety. Since approval, three cases of acute hepatitis in Austrian noni juice consumers have ...Noni juice （Morinda citrifolia） has been approved for use as a safe food within the European Union, following a review of safety. Since approval, three cases of acute hepatitis in Austrian noni juice consumers have been published, where a causal link is suggested between the liver dysfunction and ingestion of anthraquinones from the plant. Measurements of liver function in a human clinical safety study of TAHITIAN NONI Juice, as well as subacute and subchronic animal toxicity tests revealed no evidence of adverse liver effects at doses many times higher than those reported in the case studies. Additionally, M. citrifolia anthraquinones occur in the fruit in quantities too small to be of any toxicological significance. Further, these do not have chemical structures capable of being reduced to reactive anthrone radicals, which were implicated in previous cases of herbal hepototoxicity. The available data reveals no evidence of liver toxicity.展开更多
Objective:Toinvestigate the anti-angiogenic activity and antioxidant properties of Myristica fragrans（M.fragrans）（nutmeg） and Morinda citrifolia（M.citrifolia）（mengkudu） oils. Methods:The nutmeg and megkudu ess...Objective:Toinvestigate the anti-angiogenic activity and antioxidant properties of Myristica fragrans（M.fragrans）（nutmeg） and Morinda citrifolia（M.citrifolia）（mengkudu） oils. Methods:The nutmeg and megkudu essential oils were obtained by steam distillation. The antioxidant activities of both essenlial oils were delermined by beta-carotene/ linoleic acid bleaching assay and reducing power while the anti-angiogenic activity was investigated using rat aortic ring assay using various concentrations.Results:The results showed that nutmeg oil has higher antioxidant activity than mengkudu oil.The nutmeg oil effectively inhibited the oxidation of linoleic acid with（88.68±0.1）%while the inhibition percentage of oxidation of linoleic acid of the mengkudu oil is（69.44±0.4）%.The nutmeg oil and mengkudu oil showed reducing power with an EC<sub>50</sub> value of 181.4μg/mL and 3 043.0μg/mL,respectively.The anliangiogenic activity of nutmeg oil showed significant antiangiogenic activity with IC<sub>50</sub> of 77.64μg/mL comparing to mengkudu oil which exhibits IC<sub>50</sub> of 109.30μg/mL.Conclusion:Bioactive compound（s） will be isolated from the nutmeg essential oil to be developed as antiangiogenic drugs.展开更多
Objective： Aging is associated with the development of diseases because of immunosuppression and altered functioning of the neuroendocrine system. The medicinal properties of Morinda citrifolia L. have been widely ex...Objective： Aging is associated with the development of diseases because of immunosuppression and altered functioning of the neuroendocrine system. The medicinal properties of Morinda citrifolia L. have been widely exploited for the treatment of age-associated diseases. This study aims to investigate the in vitro and in vivo effects of noni（M. citrifolia） fruit juice（NFJ） on neuro-immunomodulation in the lymph node lymphocytes of F344 rats.Methods： Lymphocytes isolated from axillary and inguinal lymph nodes of young（3–4 months） and old（18–21 months） rats were treated in vitro with different concentrations（0.0001%, 0.01%, and 1%） of NFJ for a period of 24 h. In the in vivo study, old（16–17 months） male F344 rats were treated with 5 mL/kg body weight of 5%, 10% and 20% of NFJ, twice a day, by oral gavage, and lymph node lymphocytes were isolated after 60 d. Concanavalin A（Con A）-induced lymphocyte proliferation, interleukin-2（IL-2）and interferon-γ（IFN-γ） production and expression of intracellular markers, such as phosphoextracellular signal-regulated kinase（p-ERK1/2）, phospho-γ AMP response element-binding protein,phospho-protein kinase B（p-Akt）, phospho-tyrosine hydroxylase（p-TH）, phospho-nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor-α（p-IκB-α） and phospho-nuclear factor-κB（p-NF-κB p65 and p50） were examined in the lymphocytes of lymph nodes.Results： NFJ increased Con A-induced lymphocyte proliferation, IL-2 and IFN-γ production, and p-ERK1/2 expression both in vitro and in vivo. In in vivo NFJ-treated old rats, lymph node lymphocytes showed increased expression of p-TH and Akt, nitric oxide production and decreased expression of p-NF-κB p65 and p50.Conclusion： These results suggest that the immunostimulatory properties of NFJ are facilitated through intracellular signaling pathways involving ERK1/2, Akt and NF-κB.展开更多
Aim： To investigate the effect of Morinda lucida Benth （Rubiaceae） on the reproductive activity of male albino rats. Methods： Two groups of rats were treated with 400 mg/（kg.d） of Morinda lucida leaf extract for...Aim： To investigate the effect of Morinda lucida Benth （Rubiaceae） on the reproductive activity of male albino rats. Methods： Two groups of rats were treated with 400 mg/（kg.d） of Morinda lucida leaf extract for 4 and 13 weeks, respectively. The control rats received the vehicle. All the treated rats had corresponding recovery groups. At the end of each experimental period, animals were killed and organ weights, sperm characteristics, serum testosterone levels, histology of the testes and fertility were assessed. Results： Morinda lucida leaf extract did not cause any changes in body and somatic organ weights, but significantly increased the testis weight （P 〈 0.05）. The sperm motility and viability, and the epididymal sperm counts of rats treated for 13 weeks were significantly reduced （P 〈 0.05）. Sperm morphological abnormalities and serum testosterone levels were significantly increased （P 〈 0.05）. There were various degrees of damage to the seminiferous tubules. The extract reduced the fertility of the treated rats by reducing the litter size. Reversal of these changes, however, occurred after a period of time. Conclusion： The extract of Morinda lucida has reversible antispermatogenic properties.展开更多
基金supported by the National Key Research and Development Program of China(Nos.2021YFC2400600,2022YFE0203600)National Natural Science Foundation of China(Nos.81925035,82304842,82204628)+6 种基金High-level Innovative Research Institute(No.2021B0909050003)Chinese Academy of Sciences President’s International Fellowship Initiative(No.2024VBB0004)the Scientific and Technological Innovation Projects in Zhongshan City(Nos.LJ2021001,CXTD2022011)the Social Welfare and Basic Research Projects in Zhongshan(No.221014134359625)the Special Projects in Key Areas of Colleges and Universities in Guangdong Province(No.2022ZDZX2015)the Science and Technology Program of Guangzhou(No.2024A04J4899)Young Talent Project of Guangzhou University of Chinese Medicine(No.A1–2601–24–414–110Z76)。
文摘Rheumatoid arthritis(RA)is a refractory autoimmune disease with limited treatment options.Plantderived exosomes-like nanovesicles(PDENs)have emerged as a novel nanomedical approach,with the inherent bioactive compounds from their source plants.The roots of Morinda officinalis How.(MO),a Chinese herb,exhibit notable anti-inflammatory activities and hold promising therapeutic value.We engineered a joint-targe ting delivery system(termed MOE@EM)by masking MO-derived exosomes-like nanovesicles(MOE)with erythrocyte membrane(EM).This biomimetic strategy,using EM camouflage,is intended to improve the in vivo fate of MOE.We investigated the antioxidative and anti-inflammatory activities,immunogenicity,drug accumulation in the joint,and therapeutic efficacy to ascertain its suitability for RA therapy.UV irradiation significantly increased the activities of catalase and peroxidase of MOE,and enhanced the anti-inflammatory effects via the Wnt/β-catenin pathway.Furthermore,MOE@EM markedly attenuated dendritic cell activation.MOE@EM exhibited joint-specific delivery,with substantial reduction in paw swelling,and favorable modulation of immune microenvironment.
文摘Based on the meteorological data of 24 automatic stations in Xinfeng County,combined with the climatic conditions for the growth of Morinda officinalis F.C.How and Sarcandra glabra(Thunb.)Nakai,the climate suitability zoning indicators of M.officinalis and S.glabra in Xinfeng County were constructed by relevant statistical methods,and the climate suitability zoning indicators were divided into four grades:most suitable,suitable,sub-suitable and unsuitable.According to the results of expert scores,the relevant growth impact factors were assigned weight values.Based on geographic information system(GIS),the climate suitability zoning of M.officinalis and S.glabra planting in Xinfeng County was carried out.The results show that most areas of Xinfeng County were suitable for planting M.officinalis and S.glabra.Among them,the most suitable planting areas of M.officinalis were mainly distributed in most of Huilong Town,from Jiangnan Community of Fengcheng Street to Yuntianhai area of Meikeng Town,Matouwantian Village and areas to the south,and areas to the south of Zhutong Village,Fengcheng Street.The most suitable areas for S.glabra planting were mainly distributed in Puchang Village of Huilong Town,the northeast of Huangzhai Town,the south of Fengcheng Street,and the central-southern part of Matou Town.The zoning results provide a scientific basis for the rational layout of M.officinalis and S.glabra planting in Xinfeng County.
文摘The fruit of Morinda citrifolia L., commonly known as noni, has an extensive history of use as a food and traditional medicine around the world. Adding value to Morinda citrifolia L. products, particularly the fruit, could be one way of building resilience in vulnerable farming households. The aim of this study was to determine the secondary metabolite and mineral composition of noni juice obtained by fermenting the fruit of Morinda citrifolia L. Fruits were collected in August 2022 from the local field in Thiès region, West of Senegal. Extraction yields were determined and the secondary metabolites were determined using conventional analytical methods. Calcium, magnesium, iron, sodium and potassium were determined by atomic absorption spectrophotometer coupled with a CCD detector. The results show that an average fruit mass (503.2 ± 110.96 g) consists of 171.44 ± 50.01 g pulp and 34.06 ± 10.35 g seeds. The traditional extraction yield of noni juice is 16.46% after three weeks of fermentation. The contents of total polyphenols, flavonoids and tannins obtained in noni are 608.97 ± 4.53 mg EAG/100mL, 7.78 ± 0.01 mg EQ/100mL and 0.191 ± 0.01 mg EC/100mL respectively. The ethanol content of noni varies from 3.57 to 5.23 mL/100mL during extraction. Noni has a high calcium content with a concentration of 383.79 ± 33.23 mg/L. This is followed by a good concentration of magnesium, potassium and sodium, at 278.47 ± 26.30, 187.43 ± 10.7 and 155.95 ± 28.66 mg/L respectively. Noni also has an iron content of 202.15 ± 0.05 mg/L.
文摘Aim： To investigate the antioxidant effects of Morinda officinalis （Morindae radix, MR） on H2O2-induced oxidative stress in cultured mouse TM3 Leydig cells. Methods： We carried out 2,2-diphenyl-1-picrylhydrazyl free radical scavenging, 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide, lipid peroxidation, testosterone enzyme immunoassay, superoxide dismutase （SOD）, and catalase （CAT） assays in Leydig TM3 cells. Results： MR showed a 47.8% 2,2-diphenyl-1-picrylhydrazyl radical scavenging effect in TM3 cells with no significant cytotoxicity. Oxidative stress was induced in TM3 cells with 100 μmol H2O2, and treatment of the cells with 250 μg/mL MR showed the most significant protective effect （64%, P 〈 0.001） in the cell viability assay with a decreased lipid peroxidation level （1.75 nmol/mg protein, P 〈 0.05）, increased testosterone production （43.5 pg/mL）, and improvements in SOD activity （7.49 units of SOD/mg protein, P 〈 0.001） and CAT activity （74.6 units of CAT/mg protein, P 〈 0.001）. Conclusion： These findings indicate that MR, as an antioxidant, protects functions of cultured mouse TM3 Leydig cells from H2O2-induced oxidative stress.
基金supported by the Chongqing Science and Technology Commission Foundation(No.CSTC2010-AB5070)the Projects of Chongqing Science&Technology(No.CSTC2011jjA0324)+2 种基金the Southwest University Doctor Foundation(No.SWU111065)the Fundamental ResearchFunds for Central Universities(No.XDJK2010 C059)the South-west University Doctor Foundation(No.SWU112102)
文摘AIM:To explore the therapeutic effects of Morinda officinalis capsules(MOP) on osteoporosis in ovariectomized rats.METHOD:Six-month-old female Sprague–Dawley rats were induced for postmenopausal osteoporosis(PMOP) by bilateral ovariectomy and divided into seven groups as follows:sham-operated group,ovariectomized(OVX) control group,OVX treated with xianlinggubao(XLGB)(270 mg·kg-1·d-1),OVX treated with alendronate sodium(ALN)(3 mg·kg-1·d-1),and OVX treated with Morinda officinalis capsule(MOP) of graded doses(90,270 and 810 mg·kg-1·d-1) groups.Oral treatments were administered daily on the 4th week after ovariectomy and lasted for 12 weeks.The bone mineral density was evaluated by dual-energy X-ray absorptiometry.The tartrate-resistant acid phosphatase(TRAP),alkaline phosphatase(AKP),and osteocalcin(OC) levels in the serum and plasma were determined by standard colorimetric and enzyme immunoassays methods.Bone biomechanical properties and morphological parameters were analyzed by three-point bending test and histomorphometry respectively.RESULTS:Morinda officinalis capsules at all doses were able to significantly prevent the OVX-induced loss of bone mass due to diminishing serum AKP and TRAP levels while elevating OC level in the plasma.Morinda officinalis capsules also enhanced the bone strength and prevented the deterioration of trabecular microarchitecture.CONCLUSION:Morinda officinalis capsules possess potent anti-osteoporotic activity in OVX rats which could be an effective treatment for postmenopausal osteoporosis.
文摘Objective:To evaluate the antioxidant and cytotoxic effects of hexane extract of Morinda pubescens leaves and to find the primary bioactive compound responsible for antioxidant and cytotoxic activities.Methods:The individual compounds were isolated using column chromatography and were characterized by spectroscopic techniques.The antioxidant activity was evaluated for all individual isolated compounds by DPPH method using L-Ascorbic acid as standard and cytotoxicity was assessed for the extract and the hyoscyamine by MTT assay, caspase test and RT-PCR study.Results:The antioxidant activity of the isolated compounds and the extract increased as the concentration increased.One of the isolated compound hyoscyamine showed the high antioxidant activity.The extract and the hyoscyamine dose-dependently decreased the cell viability in HepG2 cells.Hyoscyamine induced caspase-3 mediated apoptosis. Up regulation of p53 gene expression provides cue for apoptotic activity of hyoscyamine. Conclusions:The results indicate that hexane extract possessed potent antioxidant and cytotoxic activity and hyoscyamine is the principal bioactive compound in hexane extract.
文摘Extraction and characterization of antioxidative compositions from the extracts of fermented Xisha Noni （Morinda citrifolia L.） juice were studied. The antioxidative constituents of 184.6 g freeze-dried extracts of naturally fermented Xisha Noni juice were isolated successfully by petroleum ether, EtOAc and n-BuOH solvents, and the antioxidative effects were measured according to scavenging activity against hydroxyl generated in Fenton reaction system and superoxide anion radicals in pyrogallol autoxidation system. The EtOAc extract exhibited most significantly higher （P 〈 0.01） antioxidative activity than mannitol or vitamin C, while the petroleum ether and n-BuOH extracts showed lower activities compared to mannitol. Three antioxidant phenolic compounds, isoscopoletin, aesculetin and 3,3＇,4＇,5,7-pentahydroxyflavone （quercetin） were isolated from the EtOAc extract by several chromatography techniques for the first time. The results suggest that several compounds, in particular, the phenolic compounds, contribute separately or synergistically to the antioxidative activity of fermented Noni fruit juice.
文摘The effect of Morinda tinctoria(MT) leaves extract on the corrosion inhibition of Al in acid medium was studied. The inhibition studies were carried out on Al in 0.5 mol/L HCl with the extract of leaves of MT using mass loss and electrochemical techniques. Parameters, such as concentration of the inhibitor, concentration of the acid, temperature and concentration of halide ions, were varied and optimized. Increase of the temperature and concentration of acid can decrease the performance of the inhibitor. Thermodynamic parameters show that the physisorption of the inhibitor molecules on Al surface obeys Langmuir isotherm.
文摘Noni juice （Morinda citrifolia） has been approved for use as a safe food within the European Union, following a review of safety. Since approval, three cases of acute hepatitis in Austrian noni juice consumers have been published, where a causal link is suggested between the liver dysfunction and ingestion of anthraquinones from the plant. Measurements of liver function in a human clinical safety study of TAHITIAN NONI Juice, as well as subacute and subchronic animal toxicity tests revealed no evidence of adverse liver effects at doses many times higher than those reported in the case studies. Additionally, M. citrifolia anthraquinones occur in the fruit in quantities too small to be of any toxicological significance. Further, these do not have chemical structures capable of being reduced to reactive anthrone radicals, which were implicated in previous cases of herbal hepototoxicity. The available data reveals no evidence of liver toxicity.
基金funded by Universiti Sains Malaysia Research University(RU)Grant(1001/PFARMASI/813021)
文摘Objective:Toinvestigate the anti-angiogenic activity and antioxidant properties of Myristica fragrans（M.fragrans）（nutmeg） and Morinda citrifolia（M.citrifolia）（mengkudu） oils. Methods:The nutmeg and megkudu essential oils were obtained by steam distillation. The antioxidant activities of both essenlial oils were delermined by beta-carotene/ linoleic acid bleaching assay and reducing power while the anti-angiogenic activity was investigated using rat aortic ring assay using various concentrations.Results:The results showed that nutmeg oil has higher antioxidant activity than mengkudu oil.The nutmeg oil effectively inhibited the oxidation of linoleic acid with（88.68±0.1）%while the inhibition percentage of oxidation of linoleic acid of the mengkudu oil is（69.44±0.4）%.The nutmeg oil and mengkudu oil showed reducing power with an EC<sub>50</sub> value of 181.4μg/mL and 3 043.0μg/mL,respectively.The anliangiogenic activity of nutmeg oil showed significant antiangiogenic activity with IC<sub>50</sub> of 77.64μg/mL comparing to mengkudu oil which exhibits IC<sub>50</sub> of 109.30μg/mL.Conclusion:Bioactive compound（s） will be isolated from the nutmeg essential oil to be developed as antiangiogenic drugs.
基金supported by a research grant(FFEP-CRP-08-002)from World Noni Research Foundation,Chennai,India
文摘Objective： Aging is associated with the development of diseases because of immunosuppression and altered functioning of the neuroendocrine system. The medicinal properties of Morinda citrifolia L. have been widely exploited for the treatment of age-associated diseases. This study aims to investigate the in vitro and in vivo effects of noni（M. citrifolia） fruit juice（NFJ） on neuro-immunomodulation in the lymph node lymphocytes of F344 rats.Methods： Lymphocytes isolated from axillary and inguinal lymph nodes of young（3–4 months） and old（18–21 months） rats were treated in vitro with different concentrations（0.0001%, 0.01%, and 1%） of NFJ for a period of 24 h. In the in vivo study, old（16–17 months） male F344 rats were treated with 5 mL/kg body weight of 5%, 10% and 20% of NFJ, twice a day, by oral gavage, and lymph node lymphocytes were isolated after 60 d. Concanavalin A（Con A）-induced lymphocyte proliferation, interleukin-2（IL-2）and interferon-γ（IFN-γ） production and expression of intracellular markers, such as phosphoextracellular signal-regulated kinase（p-ERK1/2）, phospho-γ AMP response element-binding protein,phospho-protein kinase B（p-Akt）, phospho-tyrosine hydroxylase（p-TH）, phospho-nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor-α（p-IκB-α） and phospho-nuclear factor-κB（p-NF-κB p65 and p50） were examined in the lymphocytes of lymph nodes.Results： NFJ increased Con A-induced lymphocyte proliferation, IL-2 and IFN-γ production, and p-ERK1/2 expression both in vitro and in vivo. In in vivo NFJ-treated old rats, lymph node lymphocytes showed increased expression of p-TH and Akt, nitric oxide production and decreased expression of p-NF-κB p65 and p50.Conclusion： These results suggest that the immunostimulatory properties of NFJ are facilitated through intracellular signaling pathways involving ERK1/2, Akt and NF-κB.
文摘Aim： To investigate the effect of Morinda lucida Benth （Rubiaceae） on the reproductive activity of male albino rats. Methods： Two groups of rats were treated with 400 mg/（kg.d） of Morinda lucida leaf extract for 4 and 13 weeks, respectively. The control rats received the vehicle. All the treated rats had corresponding recovery groups. At the end of each experimental period, animals were killed and organ weights, sperm characteristics, serum testosterone levels, histology of the testes and fertility were assessed. Results： Morinda lucida leaf extract did not cause any changes in body and somatic organ weights, but significantly increased the testis weight （P 〈 0.05）. The sperm motility and viability, and the epididymal sperm counts of rats treated for 13 weeks were significantly reduced （P 〈 0.05）. Sperm morphological abnormalities and serum testosterone levels were significantly increased （P 〈 0.05）. There were various degrees of damage to the seminiferous tubules. The extract reduced the fertility of the treated rats by reducing the litter size. Reversal of these changes, however, occurred after a period of time. Conclusion： The extract of Morinda lucida has reversible antispermatogenic properties.
