Nonalcoholic fatty liver disease(NAFLD) and type 2 Diabetes Mellitus(T2 DM) are highly prevalent diseases and are closely associated, with NAFLD being present in the majority of T2 DM patients. In Asian traditional me...Nonalcoholic fatty liver disease(NAFLD) and type 2 Diabetes Mellitus(T2 DM) are highly prevalent diseases and are closely associated, with NAFLD being present in the majority of T2 DM patients. In Asian traditional medicine, Mori Cortex is widely used for the treatment of diabetes and hyperlipidemia. However, whether it has a therapeutic effect on T2 DM associated with NAFLD is still unknown. The present study showed that the oral treatment with Mori Cortex extract(MCE; 10 g·kg-1·d-1) lowered the blood lipid levels and reversed insulin resistance(IR) in high fat-diet/streptozotocin-induced type 2 diabetes in rats. The expression levels of sterol receptor element-binding protein-1 c(SREBP-1 c) and carbohydrate-responsive element binding protein(Ch REBP), which are involved in steatosis in NAFLD rats, were measured in the liver samples. MCE decreased the protein and m RNA expression levels of SREBP-1 c and Ch REBP. In conclusion, down-regulation of SREBP-1 c and Ch REBP might contribute to the protective effect of MCE on hepatic injury and IR in the rats with T2 DM associated with NAFLD.展开更多
试验旨在优化桑白皮多糖(Cortex Mori polysaccharide,CMP)的提取工艺,为畜禽生产中合理利用CMP作为免疫增强剂提供参考。试验通过水提醇沉工艺提取CMP,在单因素试验的基础上,以CMP得率为评价指标设计正交试验,优选CMP提取条件。结果显...试验旨在优化桑白皮多糖(Cortex Mori polysaccharide,CMP)的提取工艺,为畜禽生产中合理利用CMP作为免疫增强剂提供参考。试验通过水提醇沉工艺提取CMP,在单因素试验的基础上,以CMP得率为评价指标设计正交试验,优选CMP提取条件。结果显示,影响CMP得率因素的主次顺序为提取温度>料液比>提取时间;CMP的最佳提取条件为提取温度90℃、液料比20 mL/g、提取时间3 h,此条件下测得桑白皮粗提物中多糖含量为59.29%,CMP得率为6.65%。研究表明,试验建立的工艺具有较高的提取效率,为CMP的进一步研究奠定了一定基础。展开更多
基金supported by Ningbo Science and Technology Innovation Team Program(No.2014B82002)the National Natural Science Foundation of China(Nos.81370165,81501421,and 31301068)+1 种基金the Natural Science Foundation of Ningbo(Nos.2013A610209 and 2015A610217)Fang Runhua Fund of Hong Kong and K.C.Wong Magna Fund of Ningbo University
文摘Nonalcoholic fatty liver disease(NAFLD) and type 2 Diabetes Mellitus(T2 DM) are highly prevalent diseases and are closely associated, with NAFLD being present in the majority of T2 DM patients. In Asian traditional medicine, Mori Cortex is widely used for the treatment of diabetes and hyperlipidemia. However, whether it has a therapeutic effect on T2 DM associated with NAFLD is still unknown. The present study showed that the oral treatment with Mori Cortex extract(MCE; 10 g·kg-1·d-1) lowered the blood lipid levels and reversed insulin resistance(IR) in high fat-diet/streptozotocin-induced type 2 diabetes in rats. The expression levels of sterol receptor element-binding protein-1 c(SREBP-1 c) and carbohydrate-responsive element binding protein(Ch REBP), which are involved in steatosis in NAFLD rats, were measured in the liver samples. MCE decreased the protein and m RNA expression levels of SREBP-1 c and Ch REBP. In conclusion, down-regulation of SREBP-1 c and Ch REBP might contribute to the protective effect of MCE on hepatic injury and IR in the rats with T2 DM associated with NAFLD.
文摘试验旨在优化桑白皮多糖(Cortex Mori polysaccharide,CMP)的提取工艺,为畜禽生产中合理利用CMP作为免疫增强剂提供参考。试验通过水提醇沉工艺提取CMP,在单因素试验的基础上,以CMP得率为评价指标设计正交试验,优选CMP提取条件。结果显示,影响CMP得率因素的主次顺序为提取温度>料液比>提取时间;CMP的最佳提取条件为提取温度90℃、液料比20 mL/g、提取时间3 h,此条件下测得桑白皮粗提物中多糖含量为59.29%,CMP得率为6.65%。研究表明,试验建立的工艺具有较高的提取效率,为CMP的进一步研究奠定了一定基础。
