为阐明克氏原螯虾(Procambarus clarkii)doublesex(PcDsx)的功能,采用RACE技术克隆获得PcDsx cDNA序列,利用qRT-PCR检测该基因的表达情况。生物信息学分析结果显示,该基因cDNA全长1584 bp,包括243 bp 5′UTR、765 bp ORF(编码254 aa)和5...为阐明克氏原螯虾(Procambarus clarkii)doublesex(PcDsx)的功能,采用RACE技术克隆获得PcDsx cDNA序列,利用qRT-PCR检测该基因的表达情况。生物信息学分析结果显示,该基因cDNA全长1584 bp,包括243 bp 5′UTR、765 bp ORF(编码254 aa)和576 bp 3′UTR;PcDsx蛋白包含1个保守的DM结构域,与东方刺龙虾(Sagmariasus verreauxi)SvDsx的DM结构域相似性较高。基因表达分析结果显示,PcDsx广泛表达于成年克氏原螯虾的各组织中,其中克氏原螯虾触角腺中该基因的相对表达量最高,性腺和肌肉中的相对表达量也较高,并且发现该基因在成年雌虾多种组织中的表达与相应雄虾组织中的表达存在显著性差异;克氏原螯虾早期发育不同时期表达分析结果表明,PcDsx的表达水平在出膜后3 d达到一个高峰,而幼虾中PcDsx的最高表达分别出现在出膜后41 d和115 d,此外,该基因在出膜后期雌雄幼虾中的表达亦表现出显著性差异。展开更多
The male giant freshwater prawn(Macrobrachium rosenbergii)generally exhibit faster growth rates compared to females of similar age.Consequently,the practice of culturing monosex(all-male)prawn can increase both the pr...The male giant freshwater prawn(Macrobrachium rosenbergii)generally exhibit faster growth rates compared to females of similar age.Consequently,the practice of culturing monosex(all-male)prawn can increase both the production yield and income.Manually selecting males for culture during the grow-out period has long been practiced in commercial prawn farming,but is labor-intensive and generally unsuccessful in producing a male monoculture.The androgenic gland(AG)is essential for sexual differentiation in prawn and development of male primary and secondary sexual characteristics.Microsurgical removal of the AG(andrectomy)from male prawn at an early development stage will transform the male into a fully-functioning neo-female.The M.rosenbergii androgenic gland-specific insulin-like hormone(Mr-IAG)plays critical roles in male spermatogenesis and male sexual development.RNA interference(RNAi)is a highly specific post-transcriptional interference or silencing of gene expression in cells.Silencing Mr-IAG had induced full and functional sex reversal of male to neo-female(genetic male with a female phenotype).Microsurgical removal of AG and RNAi are two different approaches to producing neo-females and both methods target the manipulation of AG for efficient sex-reversal.Neo-females can mate with normal males to produce all-male prawn progeny,which can be used for commercial prawn farming and to improve production efficiency and profitability.This article reviewed recent research related to the development and functions of AG and the testis and their interactions with morphological differentiation at various stages for male M.rosenbergii,aiming to increase understanding on this topic and facilitate discovery of new techniques for advancing M.rosenbergii aquaculture.展开更多
Establishing suitable salt tolerant all-male tilapia strains with fast growth rate is a crucial requirement for successful euryhaline farming required for controlling prolific breeding and as an adaptation to the effe...Establishing suitable salt tolerant all-male tilapia strains with fast growth rate is a crucial requirement for successful euryhaline farming required for controlling prolific breeding and as an adaptation to the effect of climateinduced drought.This study assessed the percentage of tilapia male hybrids,their growth performance and survival rate produced by crossing female Nile tilapia(Oreochromis niloticus)and male Rufiji tilapia(Oreochromis urolepis urolepis)at different salinities.The fingerlings were reared for 70 days in triplicate concrete tanks(1 m3),each stocked with 15 fish weighing 1.62±0.03 g,1.69±0.02 g and 1.41±0.06 g exposed to 2,15 and 35 ppt salinities,respectively.Acclimatization was done by adding seawater to freshwater at a rate of 2 ppt per day for 18 days.Fish sexing was done by visual assessment of the genital papilla followed by rearing the hybrids for six months to observe reproduction.Results showed that,the cross between O.niloticus(female)and O.urolepis urolepis(male)produced hybrids that were all-males without any reproduction within six months post hybridization.The hybrids reared at a salinity of 2 ppt had higher growth performance than those reared at 15 and 35 ppt.Similarly,hybrids cultured at 15 ppt had significantly higher growth performance than those reared at 35 ppt.All the hybrids produced had survival rates of 100%.This study indicated that,all-male tilapia produced by crossing between O.niloticus(female)and O.urolepis(male)can be used by fish farmers as alternative species for mariculture development to control prolific breeding and as an adaptation strategy to climate change.展开更多
文摘为阐明克氏原螯虾(Procambarus clarkii)doublesex(PcDsx)的功能,采用RACE技术克隆获得PcDsx cDNA序列,利用qRT-PCR检测该基因的表达情况。生物信息学分析结果显示,该基因cDNA全长1584 bp,包括243 bp 5′UTR、765 bp ORF(编码254 aa)和576 bp 3′UTR;PcDsx蛋白包含1个保守的DM结构域,与东方刺龙虾(Sagmariasus verreauxi)SvDsx的DM结构域相似性较高。基因表达分析结果显示,PcDsx广泛表达于成年克氏原螯虾的各组织中,其中克氏原螯虾触角腺中该基因的相对表达量最高,性腺和肌肉中的相对表达量也较高,并且发现该基因在成年雌虾多种组织中的表达与相应雄虾组织中的表达存在显著性差异;克氏原螯虾早期发育不同时期表达分析结果表明,PcDsx的表达水平在出膜后3 d达到一个高峰,而幼虾中PcDsx的最高表达分别出现在出膜后41 d和115 d,此外,该基因在出膜后期雌雄幼虾中的表达亦表现出显著性差异。
文摘The male giant freshwater prawn(Macrobrachium rosenbergii)generally exhibit faster growth rates compared to females of similar age.Consequently,the practice of culturing monosex(all-male)prawn can increase both the production yield and income.Manually selecting males for culture during the grow-out period has long been practiced in commercial prawn farming,but is labor-intensive and generally unsuccessful in producing a male monoculture.The androgenic gland(AG)is essential for sexual differentiation in prawn and development of male primary and secondary sexual characteristics.Microsurgical removal of the AG(andrectomy)from male prawn at an early development stage will transform the male into a fully-functioning neo-female.The M.rosenbergii androgenic gland-specific insulin-like hormone(Mr-IAG)plays critical roles in male spermatogenesis and male sexual development.RNA interference(RNAi)is a highly specific post-transcriptional interference or silencing of gene expression in cells.Silencing Mr-IAG had induced full and functional sex reversal of male to neo-female(genetic male with a female phenotype).Microsurgical removal of AG and RNAi are two different approaches to producing neo-females and both methods target the manipulation of AG for efficient sex-reversal.Neo-females can mate with normal males to produce all-male prawn progeny,which can be used for commercial prawn farming and to improve production efficiency and profitability.This article reviewed recent research related to the development and functions of AG and the testis and their interactions with morphological differentiation at various stages for male M.rosenbergii,aiming to increase understanding on this topic and facilitate discovery of new techniques for advancing M.rosenbergii aquaculture.
文摘Establishing suitable salt tolerant all-male tilapia strains with fast growth rate is a crucial requirement for successful euryhaline farming required for controlling prolific breeding and as an adaptation to the effect of climateinduced drought.This study assessed the percentage of tilapia male hybrids,their growth performance and survival rate produced by crossing female Nile tilapia(Oreochromis niloticus)and male Rufiji tilapia(Oreochromis urolepis urolepis)at different salinities.The fingerlings were reared for 70 days in triplicate concrete tanks(1 m3),each stocked with 15 fish weighing 1.62±0.03 g,1.69±0.02 g and 1.41±0.06 g exposed to 2,15 and 35 ppt salinities,respectively.Acclimatization was done by adding seawater to freshwater at a rate of 2 ppt per day for 18 days.Fish sexing was done by visual assessment of the genital papilla followed by rearing the hybrids for six months to observe reproduction.Results showed that,the cross between O.niloticus(female)and O.urolepis urolepis(male)produced hybrids that were all-males without any reproduction within six months post hybridization.The hybrids reared at a salinity of 2 ppt had higher growth performance than those reared at 15 and 35 ppt.Similarly,hybrids cultured at 15 ppt had significantly higher growth performance than those reared at 35 ppt.All the hybrids produced had survival rates of 100%.This study indicated that,all-male tilapia produced by crossing between O.niloticus(female)and O.urolepis(male)can be used by fish farmers as alternative species for mariculture development to control prolific breeding and as an adaptation strategy to climate change.
